Experiment: m.b. Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c13180 and PGA1_c13190 are separated by 11 nucleotides PGA1_c13190 and PGA1_c13200 are separated by 9 nucleotides PGA1_c13200 and PGA1_c13210 are separated by 116 nucleotides
PGA1_c13180: PGA1_c13180 - ABC transporter for D-Sorbitol, ATPase component (from data) , at 1,363,835 to 1,364,839
_c13180
PGA1_c13190: PGA1_c13190 - ABC transporter for D-Sorbitol, permease component 1 (from data) , at 1,364,851 to 1,365,681
_c13190
PGA1_c13200: PGA1_c13200 - ABC transporter for D-Sorbitol, permease component 2 (from data) , at 1,365,691 to 1,366,557
_c13200
PGA1_c13210: PGA1_c13210 - ABC transporter for D-Sorbitol, periplasmic substrate-binding component (from data) , at 1,366,674 to 1,367,975
_c13210
Position (kb)
1364
1365
1366 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1363.890 kb on - strand at 1364.093 kb on - strand, within PGA1_c13180 at 1364.098 kb on - strand, within PGA1_c13180 at 1364.169 kb on + strand, within PGA1_c13180 at 1364.190 kb on + strand, within PGA1_c13180 at 1364.190 kb on + strand, within PGA1_c13180 at 1364.258 kb on - strand, within PGA1_c13180 at 1364.349 kb on + strand, within PGA1_c13180 at 1364.433 kb on + strand, within PGA1_c13180 at 1364.433 kb on + strand, within PGA1_c13180 at 1364.436 kb on - strand, within PGA1_c13180 at 1364.441 kb on - strand, within PGA1_c13180 at 1364.441 kb on - strand, within PGA1_c13180 at 1364.482 kb on - strand, within PGA1_c13180 at 1364.665 kb on + strand, within PGA1_c13180 at 1364.681 kb on + strand, within PGA1_c13180 at 1364.823 kb on + strand at 1364.831 kb on - strand at 1364.831 kb on - strand at 1364.833 kb on + strand at 1364.841 kb on - strand at 1364.842 kb on - strand at 1364.869 kb on + strand at 1364.869 kb on + strand at 1364.869 kb on + strand at 1364.877 kb on - strand at 1364.877 kb on - strand at 1364.902 kb on - strand at 1364.919 kb on - strand at 1364.953 kb on - strand, within PGA1_c13190 at 1364.957 kb on + strand, within PGA1_c13190 at 1364.957 kb on + strand, within PGA1_c13190 at 1364.960 kb on - strand, within PGA1_c13190 at 1364.965 kb on - strand, within PGA1_c13190 at 1364.970 kb on - strand, within PGA1_c13190 at 1365.061 kb on - strand, within PGA1_c13190 at 1365.082 kb on + strand, within PGA1_c13190 at 1365.083 kb on + strand, within PGA1_c13190 at 1365.119 kb on + strand, within PGA1_c13190 at 1365.141 kb on + strand, within PGA1_c13190 at 1365.145 kb on + strand, within PGA1_c13190 at 1365.149 kb on + strand, within PGA1_c13190 at 1365.172 kb on - strand, within PGA1_c13190 at 1365.199 kb on + strand, within PGA1_c13190 at 1365.199 kb on + strand, within PGA1_c13190 at 1365.199 kb on + strand, within PGA1_c13190 at 1365.207 kb on - strand, within PGA1_c13190 at 1365.310 kb on + strand, within PGA1_c13190 at 1365.364 kb on - strand, within PGA1_c13190 at 1365.551 kb on + strand, within PGA1_c13190 at 1365.559 kb on - strand, within PGA1_c13190 at 1365.561 kb on + strand, within PGA1_c13190 at 1365.569 kb on + strand, within PGA1_c13190 at 1365.580 kb on + strand, within PGA1_c13190 at 1365.580 kb on + strand, within PGA1_c13190 at 1365.590 kb on + strand, within PGA1_c13190 at 1365.590 kb on + strand, within PGA1_c13190 at 1365.626 kb on + strand at 1365.626 kb on + strand at 1365.626 kb on + strand at 1365.626 kb on + strand at 1365.626 kb on + strand at 1365.626 kb on + strand at 1365.626 kb on + strand at 1365.626 kb on + strand at 1365.626 kb on + strand at 1365.634 kb on - strand at 1365.634 kb on - strand at 1365.634 kb on - strand at 1365.634 kb on - strand at 1365.634 kb on - strand at 1365.634 kb on - strand at 1365.634 kb on - strand at 1365.639 kb on - strand at 1365.649 kb on - strand at 1365.690 kb on + strand at 1365.826 kb on + strand, within PGA1_c13200 at 1365.849 kb on + strand, within PGA1_c13200 at 1365.995 kb on + strand, within PGA1_c13200 at 1365.995 kb on + strand, within PGA1_c13200 at 1366.003 kb on - strand, within PGA1_c13200 at 1366.003 kb on - strand, within PGA1_c13200 at 1366.003 kb on - strand, within PGA1_c13200 at 1366.039 kb on + strand, within PGA1_c13200 at 1366.047 kb on - strand, within PGA1_c13200 at 1366.100 kb on + strand, within PGA1_c13200 at 1366.100 kb on + strand, within PGA1_c13200 at 1366.103 kb on + strand, within PGA1_c13200 at 1366.117 kb on + strand, within PGA1_c13200 at 1366.197 kb on + strand, within PGA1_c13200 at 1366.311 kb on - strand, within PGA1_c13200 at 1366.344 kb on - strand, within PGA1_c13200 at 1366.347 kb on + strand, within PGA1_c13200 at 1366.355 kb on - strand, within PGA1_c13200 at 1366.401 kb on + strand, within PGA1_c13200 at 1366.411 kb on + strand, within PGA1_c13200 at 1366.411 kb on + strand, within PGA1_c13200 at 1366.424 kb on - strand, within PGA1_c13200 at 1366.427 kb on + strand, within PGA1_c13200 at 1366.480 kb on - strand at 1366.629 kb on + strand at 1366.633 kb on - strand at 1366.653 kb on + strand at 1366.661 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.00625 mg/ml remove 1,363,890 - +0.6 1,364,093 - PGA1_c13180 0.26 +0.5 1,364,098 - PGA1_c13180 0.26 +1.3 1,364,169 + PGA1_c13180 0.33 -0.4 1,364,190 + PGA1_c13180 0.35 +0.6 1,364,190 + PGA1_c13180 0.35 +0.1 1,364,258 - PGA1_c13180 0.42 -0.5 1,364,349 + PGA1_c13180 0.51 +1.2 1,364,433 + PGA1_c13180 0.60 -0.9 1,364,433 + PGA1_c13180 0.60 -1.7 1,364,436 - PGA1_c13180 0.60 -0.2 1,364,441 - PGA1_c13180 0.60 +0.1 1,364,441 - PGA1_c13180 0.60 -2.6 1,364,482 - PGA1_c13180 0.64 +0.2 1,364,665 + PGA1_c13180 0.83 -0.6 1,364,681 + PGA1_c13180 0.84 -0.9 1,364,823 + +0.3 1,364,831 - +0.7 1,364,831 - +0.3 1,364,833 + +1.2 1,364,841 - +0.2 1,364,842 - -2.5 1,364,869 + +0.0 1,364,869 + -1.2 1,364,869 + +0.5 1,364,877 - -0.1 1,364,877 - -1.7 1,364,902 - -1.1 1,364,919 - -0.5 1,364,953 - PGA1_c13190 0.12 -0.2 1,364,957 + PGA1_c13190 0.13 +0.7 1,364,957 + PGA1_c13190 0.13 +0.1 1,364,960 - PGA1_c13190 0.13 -0.5 1,364,965 - PGA1_c13190 0.14 -0.9 1,364,970 - PGA1_c13190 0.14 -0.9 1,365,061 - PGA1_c13190 0.25 +0.5 1,365,082 + PGA1_c13190 0.28 +0.2 1,365,083 + PGA1_c13190 0.28 +0.5 1,365,119 + PGA1_c13190 0.32 -1.3 1,365,141 + PGA1_c13190 0.35 -0.2 1,365,145 + PGA1_c13190 0.35 -0.9 1,365,149 + PGA1_c13190 0.36 -0.6 1,365,172 - PGA1_c13190 0.39 -1.7 1,365,199 + PGA1_c13190 0.42 -0.3 1,365,199 + PGA1_c13190 0.42 +0.0 1,365,199 + PGA1_c13190 0.42 +0.6 1,365,207 - PGA1_c13190 0.43 +0.7 1,365,310 + PGA1_c13190 0.55 -0.0 1,365,364 - PGA1_c13190 0.62 -0.7 1,365,551 + PGA1_c13190 0.84 +0.5 1,365,559 - PGA1_c13190 0.85 -0.4 1,365,561 + PGA1_c13190 0.85 -1.5 1,365,569 + PGA1_c13190 0.86 -0.9 1,365,580 + PGA1_c13190 0.88 -0.5 1,365,580 + PGA1_c13190 0.88 -1.4 1,365,590 + PGA1_c13190 0.89 -1.0 1,365,590 + PGA1_c13190 0.89 -2.5 1,365,626 + +0.5 1,365,626 + -0.9 1,365,626 + -1.5 1,365,626 + -0.2 1,365,626 + +0.6 1,365,626 + +0.0 1,365,626 + -0.5 1,365,626 + -0.4 1,365,626 + +0.6 1,365,634 - +0.5 1,365,634 - -0.7 1,365,634 - +0.1 1,365,634 - -0.4 1,365,634 - -0.4 1,365,634 - -0.4 1,365,634 - -0.9 1,365,639 - -0.6 1,365,649 - +0.5 1,365,690 + -1.2 1,365,826 + PGA1_c13200 0.16 -1.1 1,365,849 + PGA1_c13200 0.18 -0.5 1,365,995 + PGA1_c13200 0.35 +0.3 1,365,995 + PGA1_c13200 0.35 -1.1 1,366,003 - PGA1_c13200 0.36 -1.1 1,366,003 - PGA1_c13200 0.36 -1.5 1,366,003 - PGA1_c13200 0.36 -0.4 1,366,039 + PGA1_c13200 0.40 -0.5 1,366,047 - PGA1_c13200 0.41 +0.2 1,366,100 + PGA1_c13200 0.47 +0.3 1,366,100 + PGA1_c13200 0.47 -0.3 1,366,103 + PGA1_c13200 0.48 +0.1 1,366,117 + PGA1_c13200 0.49 -0.1 1,366,197 + PGA1_c13200 0.58 -0.6 1,366,311 - PGA1_c13200 0.72 -2.1 1,366,344 - PGA1_c13200 0.75 +0.5 1,366,347 + PGA1_c13200 0.76 -1.7 1,366,355 - PGA1_c13200 0.77 +1.9 1,366,401 + PGA1_c13200 0.82 +1.5 1,366,411 + PGA1_c13200 0.83 +0.9 1,366,411 + PGA1_c13200 0.83 +1.0 1,366,424 - PGA1_c13200 0.85 +1.2 1,366,427 + PGA1_c13200 0.85 -0.8 1,366,480 - -0.3 1,366,629 + -0.9 1,366,633 - +0.3 1,366,653 + -0.7 1,366,661 - -0.6
Or see this region's nucleotide sequence