Experiment: m.b. Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c11360 and PGA1_c11370 are separated by 10 nucleotides PGA1_c11370 and PGA1_c11380 are separated by 107 nucleotides
PGA1_c11360: PGA1_c11360 - Uncharacterized conserved protein, at 1,175,865 to 1,177,304
_c11360
PGA1_c11370: PGA1_c11370 - putative integral membrane protein, at 1,177,315 to 1,178,226
_c11370
PGA1_c11380: PGA1_c11380 - endonuclease-like protein, at 1,178,334 to 1,179,110
_c11380
Position (kb)
1177
1178
1179 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1176.338 kb on + strand, within PGA1_c11360 at 1176.343 kb on + strand, within PGA1_c11360 at 1176.372 kb on + strand, within PGA1_c11360 at 1176.375 kb on - strand, within PGA1_c11360 at 1176.375 kb on - strand, within PGA1_c11360 at 1176.380 kb on - strand, within PGA1_c11360 at 1176.387 kb on + strand, within PGA1_c11360 at 1176.460 kb on - strand, within PGA1_c11360 at 1176.469 kb on + strand, within PGA1_c11360 at 1176.571 kb on + strand, within PGA1_c11360 at 1176.670 kb on - strand, within PGA1_c11360 at 1176.674 kb on + strand, within PGA1_c11360 at 1176.680 kb on - strand, within PGA1_c11360 at 1176.682 kb on + strand, within PGA1_c11360 at 1176.682 kb on - strand, within PGA1_c11360 at 1176.749 kb on + strand, within PGA1_c11360 at 1176.764 kb on + strand, within PGA1_c11360 at 1176.824 kb on - strand, within PGA1_c11360 at 1176.836 kb on + strand, within PGA1_c11360 at 1176.836 kb on + strand, within PGA1_c11360 at 1176.836 kb on + strand, within PGA1_c11360 at 1177.141 kb on + strand, within PGA1_c11360 at 1177.167 kb on - strand at 1177.204 kb on - strand at 1177.277 kb on + strand at 1177.299 kb on + strand at 1177.359 kb on + strand at 1177.364 kb on + strand at 1177.364 kb on + strand at 1177.364 kb on + strand at 1177.364 kb on + strand at 1177.364 kb on + strand at 1177.364 kb on + strand at 1177.371 kb on + strand at 1177.372 kb on - strand at 1177.372 kb on - strand at 1177.372 kb on - strand at 1177.372 kb on - strand at 1177.372 kb on - strand at 1177.546 kb on - strand, within PGA1_c11370 at 1177.605 kb on + strand, within PGA1_c11370 at 1177.613 kb on - strand, within PGA1_c11370 at 1177.613 kb on - strand, within PGA1_c11370 at 1177.651 kb on - strand, within PGA1_c11370 at 1177.688 kb on - strand, within PGA1_c11370 at 1177.691 kb on + strand, within PGA1_c11370 at 1177.724 kb on + strand, within PGA1_c11370 at 1177.786 kb on + strand, within PGA1_c11370 at 1177.797 kb on + strand, within PGA1_c11370 at 1177.799 kb on - strand, within PGA1_c11370 at 1177.862 kb on - strand, within PGA1_c11370 at 1177.876 kb on + strand, within PGA1_c11370 at 1178.015 kb on + strand, within PGA1_c11370 at 1178.023 kb on - strand, within PGA1_c11370 at 1178.080 kb on + strand, within PGA1_c11370 at 1178.115 kb on + strand, within PGA1_c11370 at 1178.123 kb on - strand, within PGA1_c11370 at 1178.349 kb on - strand at 1178.377 kb on - strand at 1178.402 kb on - strand at 1178.418 kb on - strand, within PGA1_c11380 at 1178.452 kb on - strand, within PGA1_c11380 at 1178.463 kb on - strand, within PGA1_c11380 at 1178.463 kb on - strand, within PGA1_c11380 at 1178.478 kb on + strand, within PGA1_c11380 at 1178.504 kb on + strand, within PGA1_c11380 at 1178.524 kb on + strand, within PGA1_c11380 at 1178.570 kb on + strand, within PGA1_c11380 at 1178.585 kb on + strand, within PGA1_c11380 at 1178.603 kb on - strand, within PGA1_c11380 at 1178.679 kb on + strand, within PGA1_c11380 at 1178.687 kb on - strand, within PGA1_c11380 at 1178.710 kb on + strand, within PGA1_c11380 at 1178.752 kb on + strand, within PGA1_c11380 at 1178.767 kb on + strand, within PGA1_c11380 at 1178.770 kb on - strand, within PGA1_c11380 at 1178.863 kb on - strand, within PGA1_c11380 at 1178.899 kb on + strand, within PGA1_c11380 at 1178.937 kb on + strand, within PGA1_c11380 at 1178.997 kb on - strand, within PGA1_c11380 at 1179.028 kb on + strand, within PGA1_c11380 at 1179.059 kb on - strand at 1179.104 kb on + strand at 1179.112 kb on - strand at 1179.112 kb on - strand at 1179.135 kb on + strand at 1179.148 kb on + strand at 1179.174 kb on + strand at 1179.203 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.00625 mg/ml remove 1,176,338 + PGA1_c11360 0.33 -0.0 1,176,343 + PGA1_c11360 0.33 +1.0 1,176,372 + PGA1_c11360 0.35 +0.3 1,176,375 - PGA1_c11360 0.35 -1.2 1,176,375 - PGA1_c11360 0.35 +1.6 1,176,380 - PGA1_c11360 0.36 -0.0 1,176,387 + PGA1_c11360 0.36 -0.0 1,176,460 - PGA1_c11360 0.41 -1.2 1,176,469 + PGA1_c11360 0.42 +1.0 1,176,571 + PGA1_c11360 0.49 +2.6 1,176,670 - PGA1_c11360 0.56 -0.0 1,176,674 + PGA1_c11360 0.56 -1.1 1,176,680 - PGA1_c11360 0.57 +0.6 1,176,682 + PGA1_c11360 0.57 +0.2 1,176,682 - PGA1_c11360 0.57 +2.2 1,176,749 + PGA1_c11360 0.61 -1.2 1,176,764 + PGA1_c11360 0.62 +0.6 1,176,824 - PGA1_c11360 0.67 -1.4 1,176,836 + PGA1_c11360 0.67 +1.6 1,176,836 + PGA1_c11360 0.67 -2.0 1,176,836 + PGA1_c11360 0.67 -0.8 1,177,141 + PGA1_c11360 0.89 -0.8 1,177,167 - +1.6 1,177,204 - -0.8 1,177,277 + -0.4 1,177,299 + -0.8 1,177,359 + +1.6 1,177,364 + +1.6 1,177,364 + +1.6 1,177,364 + +0.6 1,177,364 + +1.6 1,177,364 + -1.4 1,177,364 + -0.8 1,177,371 + +0.6 1,177,372 - -0.2 1,177,372 - +1.6 1,177,372 - -0.8 1,177,372 - +1.0 1,177,372 - +0.2 1,177,546 - PGA1_c11370 0.25 +0.6 1,177,605 + PGA1_c11370 0.32 -0.8 1,177,613 - PGA1_c11370 0.33 -0.2 1,177,613 - PGA1_c11370 0.33 -0.4 1,177,651 - PGA1_c11370 0.37 +1.0 1,177,688 - PGA1_c11370 0.41 +0.6 1,177,691 + PGA1_c11370 0.41 -1.0 1,177,724 + PGA1_c11370 0.45 +3.6 1,177,786 + PGA1_c11370 0.52 +1.6 1,177,797 + PGA1_c11370 0.53 -0.8 1,177,799 - PGA1_c11370 0.53 -1.2 1,177,862 - PGA1_c11370 0.60 -1.6 1,177,876 + PGA1_c11370 0.62 +2.6 1,178,015 + PGA1_c11370 0.77 +1.6 1,178,023 - PGA1_c11370 0.78 -0.8 1,178,080 + PGA1_c11370 0.84 +0.2 1,178,115 + PGA1_c11370 0.88 +1.6 1,178,123 - PGA1_c11370 0.89 -1.0 1,178,349 - -0.0 1,178,377 - -0.0 1,178,402 - -1.8 1,178,418 - PGA1_c11380 0.11 +0.6 1,178,452 - PGA1_c11380 0.15 +0.3 1,178,463 - PGA1_c11380 0.17 -0.8 1,178,463 - PGA1_c11380 0.17 -0.4 1,178,478 + PGA1_c11380 0.19 +2.6 1,178,504 + PGA1_c11380 0.22 +1.6 1,178,524 + PGA1_c11380 0.24 +1.0 1,178,570 + PGA1_c11380 0.30 +0.6 1,178,585 + PGA1_c11380 0.32 +1.6 1,178,603 - PGA1_c11380 0.35 -0.0 1,178,679 + PGA1_c11380 0.44 +2.6 1,178,687 - PGA1_c11380 0.45 -1.4 1,178,710 + PGA1_c11380 0.48 -0.4 1,178,752 + PGA1_c11380 0.54 -0.4 1,178,767 + PGA1_c11380 0.56 +2.6 1,178,770 - PGA1_c11380 0.56 -0.4 1,178,863 - PGA1_c11380 0.68 +0.6 1,178,899 + PGA1_c11380 0.73 +1.6 1,178,937 + PGA1_c11380 0.78 +1.6 1,178,997 - PGA1_c11380 0.85 -0.0 1,179,028 + PGA1_c11380 0.89 +0.6 1,179,059 - -0.4 1,179,104 + +0.6 1,179,112 - -0.4 1,179,112 - -1.0 1,179,135 + +1.6 1,179,148 + +1.6 1,179,174 + -0.0 1,179,203 - +2.2
Or see this region's nucleotide sequence