Experiment: m.b. Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c11255 and PGA1_c11260 are separated by 219 nucleotides PGA1_c11260 and PGA1_c11270 are separated by 87 nucleotides PGA1_c11270 and PGA1_c11280 are separated by 211 nucleotides
PGA1_c11255: PGA1_c11255 - 50S ribosomal protein L32, at 1,164,635 to 1,164,841
_c11255
PGA1_c11260: PGA1_c11260 - Predicted metal-binding, possibly nucleic acid-binding protein, at 1,165,061 to 1,165,606
_c11260
PGA1_c11270: PGA1_c11270 - Small protein A (tmRNA-binding), at 1,165,694 to 1,166,161
_c11270
PGA1_c11280: PGA1_c11280 - Putative hemolysin, at 1,166,373 to 1,167,236
_c11280
Position (kb)
1165
1166
1167 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1165.695 kb on - strand at 1166.174 kb on + strand at 1166.182 kb on - strand at 1166.192 kb on + strand at 1166.192 kb on + strand at 1166.200 kb on - strand at 1166.244 kb on - strand at 1166.249 kb on - strand at 1166.333 kb on + strand at 1166.354 kb on + strand at 1166.354 kb on + strand at 1166.359 kb on + strand at 1166.364 kb on + strand at 1166.364 kb on + strand at 1166.367 kb on - strand at 1166.372 kb on - strand at 1166.429 kb on - strand at 1166.436 kb on - strand at 1166.436 kb on - strand at 1166.437 kb on + strand at 1166.444 kb on + strand at 1166.552 kb on + strand, within PGA1_c11280 at 1166.575 kb on + strand, within PGA1_c11280 at 1166.575 kb on + strand, within PGA1_c11280 at 1166.575 kb on + strand, within PGA1_c11280 at 1166.575 kb on + strand, within PGA1_c11280 at 1166.626 kb on + strand, within PGA1_c11280 at 1166.638 kb on - strand, within PGA1_c11280 at 1166.689 kb on + strand, within PGA1_c11280 at 1166.732 kb on + strand, within PGA1_c11280 at 1166.740 kb on - strand, within PGA1_c11280 at 1166.740 kb on - strand, within PGA1_c11280 at 1166.740 kb on - strand, within PGA1_c11280 at 1166.749 kb on + strand, within PGA1_c11280 at 1166.835 kb on + strand, within PGA1_c11280 at 1166.841 kb on - strand, within PGA1_c11280 at 1166.849 kb on + strand, within PGA1_c11280 at 1166.851 kb on - strand, within PGA1_c11280 at 1166.903 kb on - strand, within PGA1_c11280 at 1166.931 kb on - strand, within PGA1_c11280 at 1166.938 kb on - strand, within PGA1_c11280 at 1166.978 kb on + strand, within PGA1_c11280 at 1166.995 kb on + strand, within PGA1_c11280 at 1167.042 kb on - strand, within PGA1_c11280
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.00625 mg/ml remove 1,165,695 - -1.2 1,166,174 + -1.8 1,166,182 - -1.0 1,166,192 + -2.4 1,166,192 + +0.5 1,166,200 - +0.4 1,166,244 - -1.4 1,166,249 - +0.8 1,166,333 + +2.2 1,166,354 + +0.8 1,166,354 + +1.6 1,166,359 + +0.3 1,166,364 + +0.6 1,166,364 + +1.6 1,166,367 - -0.1 1,166,372 - -0.1 1,166,429 - -0.9 1,166,436 - -2.4 1,166,436 - +0.6 1,166,437 + -0.7 1,166,444 + +0.8 1,166,552 + PGA1_c11280 0.21 +0.6 1,166,575 + PGA1_c11280 0.23 -0.5 1,166,575 + PGA1_c11280 0.23 -1.4 1,166,575 + PGA1_c11280 0.23 -0.0 1,166,575 + PGA1_c11280 0.23 -0.6 1,166,626 + PGA1_c11280 0.29 +1.3 1,166,638 - PGA1_c11280 0.31 -0.7 1,166,689 + PGA1_c11280 0.37 -0.3 1,166,732 + PGA1_c11280 0.42 +1.3 1,166,740 - PGA1_c11280 0.42 -0.2 1,166,740 - PGA1_c11280 0.42 +1.0 1,166,740 - PGA1_c11280 0.42 -0.8 1,166,749 + PGA1_c11280 0.44 -2.1 1,166,835 + PGA1_c11280 0.53 -0.0 1,166,841 - PGA1_c11280 0.54 -1.5 1,166,849 + PGA1_c11280 0.55 -1.3 1,166,851 - PGA1_c11280 0.55 -1.4 1,166,903 - PGA1_c11280 0.61 +0.3 1,166,931 - PGA1_c11280 0.65 +1.6 1,166,938 - PGA1_c11280 0.65 -0.4 1,166,978 + PGA1_c11280 0.70 -1.7 1,166,995 + PGA1_c11280 0.72 -0.7 1,167,042 - PGA1_c11280 0.77 -1.0
Or see this region's nucleotide sequence