Experiment: R2A with sodium pyruvate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3529 and Dsui_3530 are separated by 198 nucleotides Dsui_3530 and Dsui_3531 overlap by 4 nucleotides Dsui_3531 and Dsui_3532 overlap by 4 nucleotides Dsui_3532 and Dsui_3533 overlap by 1 nucleotides
Dsui_3529: Dsui_3529 - acetolactate synthase, large subunit, biosynthetic type, at 3,795,117 to 3,796,817
_3529
Dsui_3530: Dsui_3530 - DNA-directed RNA polymerase specialized sigma subunit, sigma24, at 3,797,016 to 3,797,573
_3530
Dsui_3531: Dsui_3531 - Protein of unknown function (DUF3619), at 3,797,570 to 3,797,941
_3531
Dsui_3532: Dsui_3532 - Protein of unknown function (DUF3106), at 3,797,938 to 3,798,663
_3532
Dsui_3533: Dsui_3533 - putative membrane protein/domain protein, at 3,798,663 to 3,799,142
_3533
Position (kb)
3797
3798 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 3796.878 kb on + strand at 3796.893 kb on - strand at 3796.904 kb on - strand at 3796.907 kb on + strand at 3796.907 kb on + strand at 3796.931 kb on + strand at 3796.951 kb on - strand at 3796.951 kb on - strand at 3796.952 kb on - strand at 3797.002 kb on + strand at 3797.076 kb on - strand, within Dsui_3530 at 3797.078 kb on + strand, within Dsui_3530 at 3797.109 kb on + strand, within Dsui_3530 at 3797.117 kb on - strand, within Dsui_3530 at 3797.118 kb on - strand, within Dsui_3530 at 3797.138 kb on - strand, within Dsui_3530 at 3797.197 kb on - strand, within Dsui_3530 at 3797.314 kb on + strand, within Dsui_3530 at 3797.322 kb on - strand, within Dsui_3530 at 3797.322 kb on - strand, within Dsui_3530 at 3797.322 kb on - strand at 3797.356 kb on + strand, within Dsui_3530 at 3797.356 kb on + strand, within Dsui_3530 at 3797.364 kb on - strand at 3797.374 kb on - strand, within Dsui_3530 at 3797.380 kb on - strand at 3797.421 kb on - strand at 3797.438 kb on - strand, within Dsui_3530 at 3797.527 kb on + strand at 3797.527 kb on + strand at 3797.527 kb on + strand at 3797.527 kb on + strand at 3797.560 kb on + strand at 3797.567 kb on - strand at 3797.761 kb on + strand, within Dsui_3531 at 3797.775 kb on + strand, within Dsui_3531 at 3797.779 kb on + strand, within Dsui_3531 at 3797.787 kb on - strand, within Dsui_3531 at 3797.916 kb on + strand at 3798.249 kb on + strand, within Dsui_3532 at 3798.366 kb on + strand, within Dsui_3532 at 3798.374 kb on - strand, within Dsui_3532 at 3798.478 kb on - strand, within Dsui_3532 at 3798.532 kb on + strand, within Dsui_3532 at 3798.569 kb on - strand, within Dsui_3532 at 3798.684 kb on + strand at 3798.715 kb on + strand, within Dsui_3533 at 3798.750 kb on + strand, within Dsui_3533 at 3798.856 kb on + strand, within Dsui_3533 at 3798.927 kb on + strand, within Dsui_3533 at 3798.928 kb on + strand, within Dsui_3533
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium pyruvate remove 3,796,878 + -0.3 3,796,893 - -0.2 3,796,904 - +0.8 3,796,907 + -0.5 3,796,907 + -0.7 3,796,931 + -0.1 3,796,951 - -1.6 3,796,951 - +0.9 3,796,952 - -1.5 3,797,002 + +0.1 3,797,076 - Dsui_3530 0.11 -0.1 3,797,078 + Dsui_3530 0.11 +0.3 3,797,109 + Dsui_3530 0.17 +0.0 3,797,117 - Dsui_3530 0.18 +0.2 3,797,118 - Dsui_3530 0.18 -1.0 3,797,138 - Dsui_3530 0.22 -0.2 3,797,197 - Dsui_3530 0.32 +0.6 3,797,314 + Dsui_3530 0.53 -0.7 3,797,322 - Dsui_3530 0.55 -1.1 3,797,322 - Dsui_3530 0.55 +1.2 3,797,322 - +1.8 3,797,356 + Dsui_3530 0.61 +1.8 3,797,356 + Dsui_3530 0.61 -0.6 3,797,364 - +2.2 3,797,374 - Dsui_3530 0.64 -1.4 3,797,380 - +2.8 3,797,421 - +1.2 3,797,438 - Dsui_3530 0.76 +0.8 3,797,527 + +0.5 3,797,527 + +0.5 3,797,527 + -0.4 3,797,527 + -0.8 3,797,560 + -0.3 3,797,567 - +0.0 3,797,761 + Dsui_3531 0.51 +0.2 3,797,775 + Dsui_3531 0.55 +2.2 3,797,779 + Dsui_3531 0.56 -0.7 3,797,787 - Dsui_3531 0.58 -1.6 3,797,916 + +4.1 3,798,249 + Dsui_3532 0.43 +0.8 3,798,366 + Dsui_3532 0.59 -0.2 3,798,374 - Dsui_3532 0.60 -0.7 3,798,478 - Dsui_3532 0.74 -0.9 3,798,532 + Dsui_3532 0.82 -0.7 3,798,569 - Dsui_3532 0.87 +0.7 3,798,684 + +0.0 3,798,715 + Dsui_3533 0.11 +0.1 3,798,750 + Dsui_3533 0.18 -0.2 3,798,856 + Dsui_3533 0.40 -0.7 3,798,927 + Dsui_3533 0.55 -0.3 3,798,928 + Dsui_3533 0.55 +0.5
Or see this region's nucleotide sequence