Experiment: R2A with sodium pyruvate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1593 and Dsui_1594 are separated by 84 nucleotides Dsui_1594 and Dsui_1595 are separated by 65 nucleotides Dsui_1595 and Dsui_1596 are separated by 85 nucleotides
Dsui_1593: Dsui_1593 - putative TIM-barrel protein, nifR3 family, at 1,673,766 to 1,674,797
_1593
Dsui_1594: Dsui_1594 - Factor for inversion stimulation Fis, transcriptional activator, at 1,674,882 to 1,675,121
_1594
Dsui_1595: Dsui_1595 - phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase, at 1,675,187 to 1,676,752
_1595
Dsui_1596: Dsui_1596 - phosphoribosylamine--glycine ligase, at 1,676,838 to 1,678,121
_1596
Position (kb)
1675
1676
1677 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1674.293 kb on + strand, within Dsui_1593 at 1674.296 kb on + strand, within Dsui_1593 at 1674.314 kb on + strand, within Dsui_1593 at 1674.330 kb on - strand at 1674.394 kb on - strand at 1674.411 kb on - strand at 1674.447 kb on + strand, within Dsui_1593 at 1674.622 kb on - strand, within Dsui_1593 at 1674.724 kb on + strand at 1674.736 kb on - strand at 1674.752 kb on - strand at 1674.812 kb on + strand at 1674.834 kb on + strand at 1675.106 kb on - strand at 1676.801 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium pyruvate remove 1,674,293 + Dsui_1593 0.51 -0.3 1,674,296 + Dsui_1593 0.51 +0.2 1,674,314 + Dsui_1593 0.53 -2.2 1,674,330 - +1.3 1,674,394 - +0.0 1,674,411 - +1.3 1,674,447 + Dsui_1593 0.66 -0.2 1,674,622 - Dsui_1593 0.83 +0.4 1,674,724 + +0.6 1,674,736 - -1.0 1,674,752 - -0.6 1,674,812 + -0.0 1,674,834 + +0.4 1,675,106 - +2.8 1,676,801 + -0.3
Or see this region's nucleotide sequence