Experiment: R2A with sodium pyruvate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1445 and Dsui_1446 are separated by 11 nucleotides Dsui_1446 and Dsui_1447 are separated by 212 nucleotides Dsui_1447 and Dsui_1448 are separated by 2 nucleotides
Dsui_1445: Dsui_1445 - succinyl-CoA synthetase, beta subunit, at 1,500,292 to 1,501,452
_1445
Dsui_1446: Dsui_1446 - succinyl-CoA synthetase, alpha subunit, at 1,501,464 to 1,502,360
_1446
Dsui_1447: Dsui_1447 - integral membrane protein, YjbE family, at 1,502,573 to 1,503,274
_1447
Dsui_1448: Dsui_1448 - putative ornithine cyclodeaminase, mu-crystallin, at 1,503,277 to 1,504,248
_1448
Position (kb)
1501
1502
1503 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1500.643 kb on + strand, within Dsui_1445 at 1500.643 kb on + strand, within Dsui_1445 at 1500.643 kb on + strand, within Dsui_1445 at 1500.651 kb on - strand, within Dsui_1445 at 1500.770 kb on + strand, within Dsui_1445 at 1500.923 kb on - strand, within Dsui_1445 at 1501.115 kb on + strand, within Dsui_1445 at 1501.123 kb on - strand, within Dsui_1445 at 1501.229 kb on - strand, within Dsui_1445 at 1501.412 kb on + strand at 1501.448 kb on + strand at 1501.448 kb on + strand at 1501.456 kb on - strand at 1501.493 kb on + strand at 1501.493 kb on + strand at 1501.628 kb on + strand, within Dsui_1446 at 1501.861 kb on + strand, within Dsui_1446 at 1501.869 kb on - strand, within Dsui_1446 at 1502.006 kb on + strand, within Dsui_1446 at 1502.052 kb on - strand, within Dsui_1446 at 1502.261 kb on - strand, within Dsui_1446 at 1502.502 kb on - strand at 1502.523 kb on - strand at 1502.540 kb on + strand at 1502.628 kb on - strand at 1502.686 kb on + strand, within Dsui_1447 at 1502.744 kb on - strand, within Dsui_1447 at 1502.761 kb on - strand, within Dsui_1447 at 1502.793 kb on + strand, within Dsui_1447 at 1502.898 kb on - strand, within Dsui_1447 at 1502.898 kb on - strand, within Dsui_1447
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium pyruvate remove 1,500,643 + Dsui_1445 0.30 -1.1 1,500,643 + Dsui_1445 0.30 -0.3 1,500,643 + Dsui_1445 0.30 -0.7 1,500,651 - Dsui_1445 0.31 +0.6 1,500,770 + Dsui_1445 0.41 -0.4 1,500,923 - Dsui_1445 0.54 -1.1 1,501,115 + Dsui_1445 0.71 -2.5 1,501,123 - Dsui_1445 0.72 -1.3 1,501,229 - Dsui_1445 0.81 -1.1 1,501,412 + -0.6 1,501,448 + -1.2 1,501,448 + -2.2 1,501,456 - -0.6 1,501,493 + -0.4 1,501,493 + -2.5 1,501,628 + Dsui_1446 0.18 -3.0 1,501,861 + Dsui_1446 0.44 -0.6 1,501,869 - Dsui_1446 0.45 -0.6 1,502,006 + Dsui_1446 0.60 -2.5 1,502,052 - Dsui_1446 0.66 -0.1 1,502,261 - Dsui_1446 0.89 -1.4 1,502,502 - -0.1 1,502,523 - -0.3 1,502,540 + -1.3 1,502,628 - -0.4 1,502,686 + Dsui_1447 0.16 +0.3 1,502,744 - Dsui_1447 0.24 -0.2 1,502,761 - Dsui_1447 0.27 -1.0 1,502,793 + Dsui_1447 0.31 -0.6 1,502,898 - Dsui_1447 0.46 -1.7 1,502,898 - Dsui_1447 0.46 -1.2
Or see this region's nucleotide sequence