Experiment: R2A with sodium pyruvate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0551 and Dsui_0552 are separated by 22 nucleotides Dsui_0552 and Dsui_0553 overlap by 8 nucleotides Dsui_0553 and Dsui_0554 are separated by 153 nucleotides Dsui_0554 and Dsui_0555 are separated by 8 nucleotides Dsui_0555 and Dsui_0556 are separated by 21 nucleotides
Dsui_0551: Dsui_0551 - nitrogenase molybdenum-iron cofactor biosynthesis protein NifN, at 554,745 to 556,130
_0551
Dsui_0552: Dsui_0552 - nitrogen fixation protein NifX, at 556,153 to 556,581
_0552
Dsui_0553: Dsui_0553 - putative nitrogen fixation protein, at 556,574 to 557,044
_0553
Dsui_0554: Dsui_0554 - uncharacterized conserved small protein containing a coiled-coil domain, at 557,198 to 557,395
_0554
Dsui_0555: Dsui_0555 - hypothetical protein, at 557,404 to 557,784
_0555
Dsui_0556: Dsui_0556 - ferredoxin III, nif-specific, at 557,806 to 558,105
_0556
Position (kb) 556
557
558 Strain fitness (log2 ratio) -2
-1
0
1
2 at 555.578 kb on - strand, within Dsui_0551 at 555.640 kb on - strand, within Dsui_0551 at 555.769 kb on - strand at 555.790 kb on - strand, within Dsui_0551 at 555.974 kb on + strand at 556.039 kb on - strand at 556.152 kb on - strand at 556.193 kb on + strand at 556.209 kb on - strand, within Dsui_0552 at 556.259 kb on + strand, within Dsui_0552 at 556.272 kb on - strand, within Dsui_0552 at 556.351 kb on - strand, within Dsui_0552 at 556.355 kb on + strand, within Dsui_0552 at 556.478 kb on - strand at 556.485 kb on - strand at 556.534 kb on - strand, within Dsui_0552 at 556.597 kb on - strand at 556.717 kb on + strand, within Dsui_0553 at 556.784 kb on + strand, within Dsui_0553 at 556.839 kb on - strand, within Dsui_0553 at 556.876 kb on - strand, within Dsui_0553 at 556.931 kb on + strand at 557.008 kb on - strand at 557.055 kb on - strand at 557.056 kb on - strand at 557.056 kb on - strand at 557.056 kb on - strand at 557.073 kb on + strand at 557.168 kb on - strand at 557.254 kb on - strand, within Dsui_0554 at 557.259 kb on - strand, within Dsui_0554 at 557.266 kb on - strand, within Dsui_0554 at 557.335 kb on - strand, within Dsui_0554 at 557.534 kb on - strand, within Dsui_0555 at 557.641 kb on + strand, within Dsui_0555 at 557.753 kb on + strand at 557.770 kb on + strand at 557.829 kb on + strand at 557.848 kb on + strand, within Dsui_0556 at 558.041 kb on + strand, within Dsui_0556
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium pyruvate remove 555,578 - Dsui_0551 0.60 +1.5 555,640 - Dsui_0551 0.65 +1.9 555,769 - +0.8 555,790 - Dsui_0551 0.75 +0.7 555,974 + +1.9 556,039 - +0.4 556,152 - -0.7 556,193 + +0.6 556,209 - Dsui_0552 0.13 +0.4 556,259 + Dsui_0552 0.25 -0.1 556,272 - Dsui_0552 0.28 +1.9 556,351 - Dsui_0552 0.46 -1.1 556,355 + Dsui_0552 0.47 -0.5 556,478 - +0.4 556,485 - +1.4 556,534 - Dsui_0552 0.89 +0.1 556,597 - -2.0 556,717 + Dsui_0553 0.30 -0.9 556,784 + Dsui_0553 0.45 -1.0 556,839 - Dsui_0553 0.56 -1.8 556,876 - Dsui_0553 0.64 -0.1 556,931 + +0.9 557,008 - -0.6 557,055 - -0.5 557,056 - +0.9 557,056 - +1.4 557,056 - -0.2 557,073 + +1.1 557,168 - -2.5 557,254 - Dsui_0554 0.28 -0.5 557,259 - Dsui_0554 0.31 -0.1 557,266 - Dsui_0554 0.34 -0.1 557,335 - Dsui_0554 0.69 -0.9 557,534 - Dsui_0555 0.34 +0.4 557,641 + Dsui_0555 0.62 +0.5 557,753 + -0.1 557,770 + +1.4 557,829 + -0.1 557,848 + Dsui_0556 0.14 -0.5 558,041 + Dsui_0556 0.78 -0.9
Or see this region's nucleotide sequence