Experiment: R2A with sodium pyruvate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1052 and Dsui_1053 overlap by 4 nucleotides Dsui_1053 and Dsui_1054 are separated by 108 nucleotides Dsui_1054 and Dsui_1055 are separated by 31 nucleotides Dsui_1055 and Dsui_1056 are separated by 1 nucleotides
Dsui_1052: Dsui_1052 - birA, biotin-(acetyl-CoA-carboxylase) ligase, at 1,085,680 to 1,086,555
_1052
Dsui_1053: Dsui_1053 - pantothenate kinase, type III, at 1,086,552 to 1,087,310
_1053
Dsui_1054: Dsui_1054 - pyridoxal-phosphate dependent TrpB-like enzyme, at 1,087,419 to 1,088,783
_1054
Dsui_1055: Dsui_1055 - sporulation related protein, at 1,088,815 to 1,089,660
_1055
Dsui_1056: Dsui_1056 - dephospho-CoA kinase, at 1,089,662 to 1,090,279
_1056
Position (kb)
1087
1088
1089 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1086.557 kb on - strand at 1087.280 kb on - strand at 1087.388 kb on + strand at 1087.388 kb on + strand at 1087.388 kb on + strand at 1087.396 kb on - strand at 1087.429 kb on + strand at 1087.429 kb on + strand at 1087.429 kb on + strand at 1087.429 kb on + strand at 1087.463 kb on - strand at 1087.487 kb on + strand at 1087.495 kb on - strand at 1087.495 kb on - strand at 1087.547 kb on + strand at 1087.669 kb on - strand, within Dsui_1054 at 1087.891 kb on - strand, within Dsui_1054 at 1087.906 kb on - strand, within Dsui_1054 at 1087.943 kb on - strand, within Dsui_1054 at 1087.948 kb on - strand at 1088.092 kb on + strand, within Dsui_1054 at 1088.110 kb on + strand, within Dsui_1054 at 1088.110 kb on + strand, within Dsui_1054 at 1088.121 kb on - strand, within Dsui_1054 at 1088.142 kb on - strand at 1088.316 kb on - strand, within Dsui_1054 at 1088.327 kb on - strand, within Dsui_1054 at 1088.367 kb on - strand, within Dsui_1054 at 1088.384 kb on + strand, within Dsui_1054 at 1088.392 kb on - strand, within Dsui_1054 at 1088.405 kb on - strand, within Dsui_1054 at 1088.636 kb on + strand, within Dsui_1054 at 1088.644 kb on - strand, within Dsui_1054 at 1088.644 kb on - strand, within Dsui_1054 at 1088.649 kb on - strand at 1088.713 kb on + strand at 1088.755 kb on + strand at 1088.755 kb on + strand at 1089.162 kb on + strand, within Dsui_1055 at 1089.255 kb on + strand, within Dsui_1055 at 1089.383 kb on + strand, within Dsui_1055 at 1089.472 kb on - strand, within Dsui_1055 at 1089.492 kb on + strand, within Dsui_1055 at 1089.492 kb on + strand, within Dsui_1055 at 1089.500 kb on - strand, within Dsui_1055 at 1089.630 kb on - strand at 1089.649 kb on + strand at 1089.654 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium pyruvate remove 1,086,557 - +3.0 1,087,280 - +3.0 1,087,388 + -0.0 1,087,388 + +0.3 1,087,388 + +0.2 1,087,396 - -0.4 1,087,429 + +2.4 1,087,429 + +0.8 1,087,429 + -0.4 1,087,429 + -0.9 1,087,463 - +1.4 1,087,487 + +0.5 1,087,495 - -0.2 1,087,495 - +0.4 1,087,547 + -0.2 1,087,669 - Dsui_1054 0.18 +0.6 1,087,891 - Dsui_1054 0.35 +0.8 1,087,906 - Dsui_1054 0.36 +1.3 1,087,943 - Dsui_1054 0.38 +1.3 1,087,948 - +2.0 1,088,092 + Dsui_1054 0.49 +0.2 1,088,110 + Dsui_1054 0.51 +1.1 1,088,110 + Dsui_1054 0.51 +0.8 1,088,121 - Dsui_1054 0.51 -0.3 1,088,142 - +1.0 1,088,316 - Dsui_1054 0.66 -0.1 1,088,327 - Dsui_1054 0.67 +3.3 1,088,367 - Dsui_1054 0.69 -1.6 1,088,384 + Dsui_1054 0.71 +0.1 1,088,392 - Dsui_1054 0.71 -1.2 1,088,405 - Dsui_1054 0.72 +0.4 1,088,636 + Dsui_1054 0.89 +0.4 1,088,644 - Dsui_1054 0.90 -0.3 1,088,644 - Dsui_1054 0.90 +0.7 1,088,649 - -0.0 1,088,713 + -0.1 1,088,755 + +0.5 1,088,755 + +1.1 1,089,162 + Dsui_1055 0.41 +0.4 1,089,255 + Dsui_1055 0.52 +0.5 1,089,383 + Dsui_1055 0.67 -1.8 1,089,472 - Dsui_1055 0.78 -0.0 1,089,492 + Dsui_1055 0.80 -0.1 1,089,492 + Dsui_1055 0.80 -0.5 1,089,500 - Dsui_1055 0.81 +0.0 1,089,630 - +1.0 1,089,649 + +0.9 1,089,654 + -0.7
Or see this region's nucleotide sequence