Experiment: R2A with sodium pyruvate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1051 and Dsui_1052 are separated by 60 nucleotides Dsui_1052 and Dsui_1053 overlap by 4 nucleotides Dsui_1053 and Dsui_1054 are separated by 108 nucleotides
Dsui_1051: Dsui_1051 - nitrogen regulation protein NR(I), at 1,084,183 to 1,085,619
_1051
Dsui_1052: Dsui_1052 - birA, biotin-(acetyl-CoA-carboxylase) ligase, at 1,085,680 to 1,086,555
_1052
Dsui_1053: Dsui_1053 - pantothenate kinase, type III, at 1,086,552 to 1,087,310
_1053
Dsui_1054: Dsui_1054 - pyridoxal-phosphate dependent TrpB-like enzyme, at 1,087,419 to 1,088,783
_1054
Position (kb)
1085
1086
1087 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1084.727 kb on + strand, within Dsui_1051 at 1084.735 kb on - strand, within Dsui_1051 at 1084.738 kb on + strand, within Dsui_1051 at 1084.853 kb on + strand, within Dsui_1051 at 1084.863 kb on + strand, within Dsui_1051 at 1084.871 kb on - strand, within Dsui_1051 at 1085.073 kb on + strand, within Dsui_1051 at 1085.162 kb on + strand, within Dsui_1051 at 1085.169 kb on - strand, within Dsui_1051 at 1085.523 kb on + strand at 1085.575 kb on - strand at 1085.585 kb on - strand at 1085.593 kb on - strand at 1085.664 kb on + strand at 1085.671 kb on + strand at 1085.672 kb on - strand at 1085.674 kb on - strand at 1085.737 kb on - strand at 1085.748 kb on + strand at 1085.789 kb on - strand at 1086.557 kb on - strand at 1087.280 kb on - strand at 1087.388 kb on + strand at 1087.388 kb on + strand at 1087.388 kb on + strand at 1087.396 kb on - strand at 1087.429 kb on + strand at 1087.429 kb on + strand at 1087.429 kb on + strand at 1087.429 kb on + strand at 1087.463 kb on - strand at 1087.487 kb on + strand at 1087.495 kb on - strand at 1087.495 kb on - strand at 1087.547 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium pyruvate remove 1,084,727 + Dsui_1051 0.38 -0.3 1,084,735 - Dsui_1051 0.38 +0.7 1,084,738 + Dsui_1051 0.39 -0.4 1,084,853 + Dsui_1051 0.47 +2.1 1,084,863 + Dsui_1051 0.47 +1.7 1,084,871 - Dsui_1051 0.48 +0.3 1,085,073 + Dsui_1051 0.62 +1.7 1,085,162 + Dsui_1051 0.68 +0.1 1,085,169 - Dsui_1051 0.69 -0.1 1,085,523 + +1.2 1,085,575 - +0.3 1,085,585 - -1.0 1,085,593 - +0.0 1,085,664 + +0.7 1,085,671 + -0.7 1,085,672 - -0.2 1,085,674 - -0.6 1,085,737 - +0.4 1,085,748 + -1.0 1,085,789 - +2.0 1,086,557 - +3.0 1,087,280 - +3.0 1,087,388 + -0.0 1,087,388 + +0.3 1,087,388 + +0.2 1,087,396 - -0.4 1,087,429 + +2.4 1,087,429 + +0.8 1,087,429 + -0.4 1,087,429 + -0.9 1,087,463 - +1.4 1,087,487 + +0.5 1,087,495 - -0.2 1,087,495 - +0.4 1,087,547 + -0.2
Or see this region's nucleotide sequence