Experiment: R2A with sodium pyruvate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1050 and Dsui_1051 are separated by 79 nucleotides Dsui_1051 and Dsui_1052 are separated by 60 nucleotides Dsui_1052 and Dsui_1053 overlap by 4 nucleotides
Dsui_1050: Dsui_1050 - signal transduction histidine kinase, nitrogen specific, at 1,083,048 to 1,084,103
_1050
Dsui_1051: Dsui_1051 - nitrogen regulation protein NR(I), at 1,084,183 to 1,085,619
_1051
Dsui_1052: Dsui_1052 - birA, biotin-(acetyl-CoA-carboxylase) ligase, at 1,085,680 to 1,086,555
_1052
Dsui_1053: Dsui_1053 - pantothenate kinase, type III, at 1,086,552 to 1,087,310
_1053
Position (kb)
1084
1085
1086 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1083.193 kb on + strand, within Dsui_1050 at 1083.234 kb on - strand, within Dsui_1050 at 1083.245 kb on - strand, within Dsui_1050 at 1083.249 kb on + strand, within Dsui_1050 at 1083.249 kb on + strand at 1083.249 kb on + strand, within Dsui_1050 at 1083.257 kb on - strand, within Dsui_1050 at 1083.266 kb on + strand, within Dsui_1050 at 1083.266 kb on + strand, within Dsui_1050 at 1083.274 kb on - strand, within Dsui_1050 at 1083.395 kb on - strand, within Dsui_1050 at 1083.406 kb on + strand at 1083.406 kb on + strand, within Dsui_1050 at 1083.442 kb on + strand, within Dsui_1050 at 1083.450 kb on - strand, within Dsui_1050 at 1083.535 kb on + strand, within Dsui_1050 at 1083.535 kb on + strand, within Dsui_1050 at 1083.538 kb on - strand, within Dsui_1050 at 1083.539 kb on - strand, within Dsui_1050 at 1083.541 kb on + strand, within Dsui_1050 at 1083.543 kb on - strand, within Dsui_1050 at 1083.543 kb on - strand, within Dsui_1050 at 1083.543 kb on - strand, within Dsui_1050 at 1083.555 kb on + strand, within Dsui_1050 at 1083.579 kb on - strand, within Dsui_1050 at 1083.657 kb on + strand, within Dsui_1050 at 1083.666 kb on + strand, within Dsui_1050 at 1083.723 kb on + strand, within Dsui_1050 at 1083.775 kb on - strand, within Dsui_1050 at 1083.775 kb on - strand, within Dsui_1050 at 1083.787 kb on - strand, within Dsui_1050 at 1083.806 kb on + strand, within Dsui_1050 at 1083.822 kb on + strand, within Dsui_1050 at 1083.927 kb on + strand, within Dsui_1050 at 1084.012 kb on - strand at 1084.136 kb on + strand at 1084.138 kb on + strand at 1084.151 kb on - strand at 1084.183 kb on + strand at 1084.270 kb on + strand at 1084.363 kb on + strand, within Dsui_1051 at 1084.371 kb on - strand, within Dsui_1051 at 1084.371 kb on - strand, within Dsui_1051 at 1084.372 kb on - strand, within Dsui_1051 at 1084.397 kb on + strand, within Dsui_1051 at 1084.411 kb on - strand, within Dsui_1051 at 1084.443 kb on - strand at 1084.511 kb on + strand, within Dsui_1051 at 1084.516 kb on + strand, within Dsui_1051 at 1084.521 kb on + strand, within Dsui_1051 at 1084.602 kb on + strand, within Dsui_1051 at 1084.610 kb on - strand at 1084.727 kb on + strand, within Dsui_1051 at 1084.735 kb on - strand, within Dsui_1051 at 1084.738 kb on + strand, within Dsui_1051 at 1084.853 kb on + strand, within Dsui_1051 at 1084.863 kb on + strand, within Dsui_1051 at 1084.871 kb on - strand, within Dsui_1051 at 1085.073 kb on + strand, within Dsui_1051 at 1085.162 kb on + strand, within Dsui_1051 at 1085.169 kb on - strand, within Dsui_1051 at 1085.523 kb on + strand at 1085.575 kb on - strand at 1085.585 kb on - strand at 1085.593 kb on - strand at 1085.664 kb on + strand at 1085.671 kb on + strand at 1085.672 kb on - strand at 1085.674 kb on - strand at 1085.737 kb on - strand at 1085.748 kb on + strand at 1085.789 kb on - strand at 1086.557 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium pyruvate remove 1,083,193 + Dsui_1050 0.14 -1.1 1,083,234 - Dsui_1050 0.18 +0.9 1,083,245 - Dsui_1050 0.19 +0.9 1,083,249 + Dsui_1050 0.19 +0.2 1,083,249 + +2.7 1,083,249 + Dsui_1050 0.19 +0.4 1,083,257 - Dsui_1050 0.20 -0.1 1,083,266 + Dsui_1050 0.21 -0.2 1,083,266 + Dsui_1050 0.21 +0.4 1,083,274 - Dsui_1050 0.21 -0.5 1,083,395 - Dsui_1050 0.33 -0.7 1,083,406 + +2.4 1,083,406 + Dsui_1050 0.34 +0.4 1,083,442 + Dsui_1050 0.37 -0.3 1,083,450 - Dsui_1050 0.38 +0.0 1,083,535 + Dsui_1050 0.46 -0.7 1,083,535 + Dsui_1050 0.46 -0.4 1,083,538 - Dsui_1050 0.46 +0.6 1,083,539 - Dsui_1050 0.46 -0.7 1,083,541 + Dsui_1050 0.47 -2.0 1,083,543 - Dsui_1050 0.47 +0.6 1,083,543 - Dsui_1050 0.47 -0.2 1,083,543 - Dsui_1050 0.47 -0.1 1,083,555 + Dsui_1050 0.48 -1.4 1,083,579 - Dsui_1050 0.50 +1.1 1,083,657 + Dsui_1050 0.58 +0.7 1,083,666 + Dsui_1050 0.59 +0.2 1,083,723 + Dsui_1050 0.64 -0.6 1,083,775 - Dsui_1050 0.69 +0.0 1,083,775 - Dsui_1050 0.69 -1.3 1,083,787 - Dsui_1050 0.70 +0.6 1,083,806 + Dsui_1050 0.72 -0.1 1,083,822 + Dsui_1050 0.73 +0.2 1,083,927 + Dsui_1050 0.83 +0.3 1,084,012 - +0.4 1,084,136 + +0.6 1,084,138 + +1.3 1,084,151 - +0.0 1,084,183 + +0.7 1,084,270 + -0.3 1,084,363 + Dsui_1051 0.13 +0.1 1,084,371 - Dsui_1051 0.13 +0.0 1,084,371 - Dsui_1051 0.13 +0.3 1,084,372 - Dsui_1051 0.13 +0.8 1,084,397 + Dsui_1051 0.15 +0.7 1,084,411 - Dsui_1051 0.16 +2.7 1,084,443 - +1.6 1,084,511 + Dsui_1051 0.23 +2.0 1,084,516 + Dsui_1051 0.23 -0.3 1,084,521 + Dsui_1051 0.24 +0.5 1,084,602 + Dsui_1051 0.29 -0.8 1,084,610 - +1.0 1,084,727 + Dsui_1051 0.38 -0.3 1,084,735 - Dsui_1051 0.38 +0.7 1,084,738 + Dsui_1051 0.39 -0.4 1,084,853 + Dsui_1051 0.47 +2.1 1,084,863 + Dsui_1051 0.47 +1.7 1,084,871 - Dsui_1051 0.48 +0.3 1,085,073 + Dsui_1051 0.62 +1.7 1,085,162 + Dsui_1051 0.68 +0.1 1,085,169 - Dsui_1051 0.69 -0.1 1,085,523 + +1.2 1,085,575 - +0.3 1,085,585 - -1.0 1,085,593 - +0.0 1,085,664 + +0.7 1,085,671 + -0.7 1,085,672 - -0.2 1,085,674 - -0.6 1,085,737 - +0.4 1,085,748 + -1.0 1,085,789 - +2.0 1,086,557 - +3.0
Or see this region's nucleotide sequence