Experiment: R2A with sodium pyruvate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0434 and Dsui_0435 are separated by 64 nucleotides Dsui_0435 and Dsui_0436 are separated by 227 nucleotides
Dsui_0434: Dsui_0434 - ribonuclease, Rne/Rng family, at 440,658 to 442,139
_0434
Dsui_0435: Dsui_0435 - Sel1 repeat protein, at 442,204 to 442,791
_0435
Dsui_0436: Dsui_0436 - dienelactone hydrolase-like enzyme, at 443,019 to 443,903
_0436
Position (kb) 442
443 Strain fitness (log2 ratio) -1
0
1
2
3 at 441.408 kb on + strand, within Dsui_0434 at 441.416 kb on - strand at 441.454 kb on - strand at 441.504 kb on + strand, within Dsui_0434 at 441.745 kb on - strand at 441.798 kb on + strand, within Dsui_0434 at 441.882 kb on + strand, within Dsui_0434 at 441.923 kb on - strand, within Dsui_0434 at 442.077 kb on + strand at 442.133 kb on + strand at 442.285 kb on + strand, within Dsui_0435 at 442.346 kb on - strand, within Dsui_0435 at 442.453 kb on - strand, within Dsui_0435 at 442.491 kb on + strand, within Dsui_0435 at 442.572 kb on - strand, within Dsui_0435 at 442.594 kb on + strand, within Dsui_0435 at 442.599 kb on + strand at 442.747 kb on + strand at 442.747 kb on + strand at 442.771 kb on + strand at 442.802 kb on + strand at 442.810 kb on - strand at 442.957 kb on + strand at 443.130 kb on - strand, within Dsui_0436 at 443.135 kb on - strand, within Dsui_0436 at 443.264 kb on + strand at 443.331 kb on + strand, within Dsui_0436 at 443.340 kb on + strand, within Dsui_0436 at 443.401 kb on - strand, within Dsui_0436 at 443.469 kb on + strand, within Dsui_0436 at 443.545 kb on - strand, within Dsui_0436 at 443.729 kb on - strand, within Dsui_0436 at 443.753 kb on + strand, within Dsui_0436 at 443.753 kb on + strand, within Dsui_0436
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium pyruvate remove 441,408 + Dsui_0434 0.51 +0.4 441,416 - +0.6 441,454 - +0.6 441,504 + Dsui_0434 0.57 +0.5 441,745 - +3.0 441,798 + Dsui_0434 0.77 +0.1 441,882 + Dsui_0434 0.83 -0.5 441,923 - Dsui_0434 0.85 -1.0 442,077 + +0.8 442,133 + -0.5 442,285 + Dsui_0435 0.14 -0.8 442,346 - Dsui_0435 0.24 -0.3 442,453 - Dsui_0435 0.42 -0.1 442,491 + Dsui_0435 0.49 +0.2 442,572 - Dsui_0435 0.63 +0.5 442,594 + Dsui_0435 0.66 +0.1 442,599 + +1.4 442,747 + +0.1 442,747 + +1.4 442,771 + +1.6 442,802 + +1.9 442,810 - +0.6 442,957 + +1.4 443,130 - Dsui_0436 0.13 +0.1 443,135 - Dsui_0436 0.13 +0.7 443,264 + +1.4 443,331 + Dsui_0436 0.35 +0.0 443,340 + Dsui_0436 0.36 -1.1 443,401 - Dsui_0436 0.43 -1.0 443,469 + Dsui_0436 0.51 +0.5 443,545 - Dsui_0436 0.59 -0.3 443,729 - Dsui_0436 0.80 -0.3 443,753 + Dsui_0436 0.83 -0.3 443,753 + Dsui_0436 0.83 -0.5
Or see this region's nucleotide sequence