Experiment: Thymidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_0060 and Sama_0061 are separated by 273 nucleotides Sama_0061 and Sama_0062 are separated by 219 nucleotides Sama_0062 and gpsA are separated by 6 nucleotides
Sama_0060: Sama_0060 - phosphoglyceromutase (RefSeq), at 65,149 to 66,690
_0060
Sama_0061: Sama_0061 - rhodanese-like protein (RefSeq), at 66,964 to 67,356
_0061
Sama_0062: Sama_0062 - preprotein translocase subunit SecB (RefSeq), at 67,576 to 68,055
_0062
Sama_0063: gpsA - NAD(P)H-dependent glycerol-3-phosphate dehydrogenase (RefSeq), at 68,062 to 69,081
gpsA
Position (kb) 67
68
69 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 66.715 kb on - strand at 66.752 kb on + strand at 66.752 kb on + strand at 66.752 kb on + strand at 66.752 kb on + strand at 66.752 kb on + strand at 66.753 kb on - strand at 66.753 kb on - strand at 66.755 kb on + strand at 66.755 kb on + strand at 66.756 kb on - strand at 66.756 kb on - strand at 66.758 kb on + strand at 66.758 kb on + strand at 66.759 kb on - strand at 66.759 kb on - strand at 66.832 kb on + strand at 66.833 kb on - strand at 66.833 kb on - strand at 66.833 kb on - strand at 66.876 kb on + strand at 66.880 kb on + strand at 66.880 kb on + strand at 66.880 kb on + strand at 66.880 kb on + strand at 66.880 kb on + strand at 66.880 kb on + strand at 66.880 kb on + strand at 66.880 kb on + strand at 66.880 kb on + strand at 66.881 kb on - strand at 66.881 kb on - strand at 66.881 kb on - strand at 66.881 kb on - strand at 66.881 kb on - strand at 66.881 kb on - strand at 66.882 kb on + strand at 66.882 kb on + strand at 66.883 kb on - strand at 66.883 kb on - strand at 66.883 kb on - strand at 66.907 kb on + strand at 66.908 kb on - strand at 66.913 kb on + strand at 66.914 kb on - strand at 66.914 kb on - strand at 66.924 kb on + strand at 66.924 kb on + strand at 66.937 kb on + strand at 66.937 kb on + strand at 66.937 kb on + strand at 66.938 kb on - strand at 66.938 kb on - strand at 67.008 kb on + strand, within Sama_0061 at 67.008 kb on + strand, within Sama_0061 at 67.008 kb on + strand, within Sama_0061 at 67.009 kb on - strand, within Sama_0061 at 67.009 kb on - strand, within Sama_0061 at 67.017 kb on + strand, within Sama_0061 at 67.017 kb on + strand, within Sama_0061 at 67.023 kb on + strand, within Sama_0061 at 67.024 kb on - strand, within Sama_0061 at 67.024 kb on - strand, within Sama_0061 at 67.024 kb on - strand, within Sama_0061 at 67.024 kb on - strand, within Sama_0061 at 67.097 kb on + strand, within Sama_0061 at 67.098 kb on - strand, within Sama_0061 at 67.098 kb on - strand, within Sama_0061 at 67.111 kb on - strand, within Sama_0061 at 67.218 kb on + strand, within Sama_0061 at 67.218 kb on + strand, within Sama_0061 at 67.218 kb on + strand, within Sama_0061 at 67.218 kb on + strand, within Sama_0061 at 67.218 kb on + strand, within Sama_0061 at 67.218 kb on + strand, within Sama_0061 at 67.218 kb on + strand, within Sama_0061 at 67.218 kb on + strand, within Sama_0061 at 67.218 kb on + strand, within Sama_0061 at 67.220 kb on + strand, within Sama_0061 at 67.220 kb on + strand, within Sama_0061 at 67.220 kb on + strand, within Sama_0061 at 67.220 kb on + strand, within Sama_0061 at 67.220 kb on + strand, within Sama_0061 at 67.220 kb on + strand, within Sama_0061 at 67.220 kb on + strand, within Sama_0061 at 67.292 kb on + strand, within Sama_0061 at 67.292 kb on + strand, within Sama_0061 at 67.292 kb on + strand, within Sama_0061 at 67.292 kb on + strand, within Sama_0061 at 67.292 kb on + strand, within Sama_0061 at 67.292 kb on + strand, within Sama_0061 at 67.294 kb on + strand, within Sama_0061 at 67.294 kb on + strand, within Sama_0061 at 67.294 kb on + strand, within Sama_0061 at 67.294 kb on + strand, within Sama_0061 at 67.294 kb on + strand, within Sama_0061 at 67.311 kb on + strand, within Sama_0061 at 67.354 kb on + strand at 67.408 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Thymidine remove 66,715 - +1.2 66,752 + -0.8 66,752 + +0.9 66,752 + -1.6 66,752 + -1.3 66,752 + -2.2 66,753 - +2.7 66,753 - +0.5 66,755 + -3.3 66,755 + -3.1 66,756 - -0.4 66,756 - -1.3 66,758 + -1.7 66,758 + -2.3 66,759 - -1.3 66,759 - -1.3 66,832 + +0.6 66,833 - +1.4 66,833 - -0.1 66,833 - +0.3 66,876 + +0.5 66,880 + -1.0 66,880 + +0.4 66,880 + -0.4 66,880 + -1.3 66,880 + -0.7 66,880 + -0.1 66,880 + +0.2 66,880 + -1.3 66,880 + -0.1 66,881 - +0.4 66,881 - +0.5 66,881 - -0.5 66,881 - -0.1 66,881 - +1.5 66,881 - +1.2 66,882 + +0.7 66,882 + +0.3 66,883 - -0.9 66,883 - +0.7 66,883 - -0.3 66,907 + +0.7 66,908 - -0.3 66,913 + -0.1 66,914 - -0.8 66,914 - -0.7 66,924 + -0.3 66,924 + +0.4 66,937 + -2.3 66,937 + -0.1 66,937 + -0.6 66,938 - -0.8 66,938 - -1.1 67,008 + Sama_0061 0.11 +1.3 67,008 + Sama_0061 0.11 -1.6 67,008 + Sama_0061 0.11 -1.3 67,009 - Sama_0061 0.11 -0.8 67,009 - Sama_0061 0.11 -1.6 67,017 + Sama_0061 0.13 -0.0 67,017 + Sama_0061 0.13 -2.1 67,023 + Sama_0061 0.15 -1.1 67,024 - Sama_0061 0.15 +0.0 67,024 - Sama_0061 0.15 -0.3 67,024 - Sama_0061 0.15 -0.8 67,024 - Sama_0061 0.15 +0.2 67,097 + Sama_0061 0.34 +1.0 67,098 - Sama_0061 0.34 +0.7 67,098 - Sama_0061 0.34 -2.3 67,111 - Sama_0061 0.37 -0.2 67,218 + Sama_0061 0.65 +0.2 67,218 + Sama_0061 0.65 +0.1 67,218 + Sama_0061 0.65 +0.1 67,218 + Sama_0061 0.65 -0.2 67,218 + Sama_0061 0.65 -0.1 67,218 + Sama_0061 0.65 -1.0 67,218 + Sama_0061 0.65 -2.3 67,218 + Sama_0061 0.65 -1.5 67,218 + Sama_0061 0.65 +0.3 67,220 + Sama_0061 0.65 -0.7 67,220 + Sama_0061 0.65 +1.7 67,220 + Sama_0061 0.65 -0.2 67,220 + Sama_0061 0.65 -0.3 67,220 + Sama_0061 0.65 -0.6 67,220 + Sama_0061 0.65 -0.3 67,220 + Sama_0061 0.65 +1.1 67,292 + Sama_0061 0.83 +0.2 67,292 + Sama_0061 0.83 -0.3 67,292 + Sama_0061 0.83 -0.1 67,292 + Sama_0061 0.83 -0.0 67,292 + Sama_0061 0.83 -0.3 67,292 + Sama_0061 0.83 +0.2 67,294 + Sama_0061 0.84 +0.2 67,294 + Sama_0061 0.84 -1.4 67,294 + Sama_0061 0.84 -1.1 67,294 + Sama_0061 0.84 -1.6 67,294 + Sama_0061 0.84 +0.7 67,311 + Sama_0061 0.88 -0.8 67,354 + -0.6 67,408 + -1.8
Or see this region's nucleotide sequence