Experiment: m.b. Phosphomycin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_237 and HP15_238 overlap by 4 nucleotides HP15_238 and HP15_239 are separated by 8 nucleotides HP15_239 and HP15_240 overlap by 20 nucleotides
HP15_237: HP15_237 - HemY domain protein, at 222,307 to 223,548
_237
HP15_238: HP15_238 - protein of unknown function, HemX, at 223,545 to 224,669
_238
HP15_239: HP15_239 - uroporphyrinogen-III synthase, at 224,678 to 225,487
_239
HP15_240: HP15_240 - porphobilinogen deaminase, at 225,468 to 226,409
_240
Position (kb)
223
224
225 Strain fitness (log2 ratio)
-2
-1
0
1 at 222.640 kb on - strand, within HP15_237 at 222.651 kb on - strand, within HP15_237 at 222.665 kb on + strand, within HP15_237 at 222.715 kb on - strand, within HP15_237 at 222.777 kb on + strand, within HP15_237 at 222.843 kb on + strand, within HP15_237 at 222.848 kb on + strand, within HP15_237 at 222.920 kb on + strand, within HP15_237 at 223.035 kb on + strand, within HP15_237 at 223.071 kb on + strand, within HP15_237 at 223.103 kb on - strand, within HP15_237 at 223.108 kb on + strand, within HP15_237 at 223.108 kb on + strand, within HP15_237 at 223.108 kb on + strand, within HP15_237 at 223.189 kb on + strand, within HP15_237 at 223.281 kb on - strand, within HP15_237 at 223.426 kb on - strand at 223.464 kb on - strand at 223.472 kb on + strand at 223.497 kb on + strand at 223.500 kb on - strand at 223.640 kb on - strand at 223.700 kb on + strand, within HP15_238 at 223.710 kb on - strand, within HP15_238 at 223.893 kb on - strand, within HP15_238 at 223.898 kb on + strand, within HP15_238 at 223.908 kb on + strand, within HP15_238 at 223.939 kb on - strand, within HP15_238 at 223.939 kb on - strand, within HP15_238 at 223.953 kb on + strand, within HP15_238 at 224.038 kb on - strand, within HP15_238 at 224.040 kb on + strand, within HP15_238 at 224.048 kb on - strand, within HP15_238 at 224.054 kb on - strand, within HP15_238 at 224.523 kb on - strand, within HP15_238
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Phosphomycin 1 mg/ml remove 222,640 - HP15_237 0.27 +1.2 222,651 - HP15_237 0.28 -0.8 222,665 + HP15_237 0.29 +0.2 222,715 - HP15_237 0.33 -0.4 222,777 + HP15_237 0.38 -1.2 222,843 + HP15_237 0.43 -0.2 222,848 + HP15_237 0.44 +0.4 222,920 + HP15_237 0.49 -0.3 223,035 + HP15_237 0.59 -1.8 223,071 + HP15_237 0.62 +0.1 223,103 - HP15_237 0.64 +0.3 223,108 + HP15_237 0.64 -0.8 223,108 + HP15_237 0.64 -0.7 223,108 + HP15_237 0.64 -2.7 223,189 + HP15_237 0.71 -0.7 223,281 - HP15_237 0.78 -0.3 223,426 - -0.6 223,464 - -0.5 223,472 + +0.5 223,497 + +0.5 223,500 - -2.0 223,640 - +0.5 223,700 + HP15_238 0.14 -1.4 223,710 - HP15_238 0.15 -0.1 223,893 - HP15_238 0.31 +1.1 223,898 + HP15_238 0.31 -0.4 223,908 + HP15_238 0.32 -1.2 223,939 - HP15_238 0.35 -0.4 223,939 - HP15_238 0.35 -0.7 223,953 + HP15_238 0.36 -1.6 224,038 - HP15_238 0.44 -0.1 224,040 + HP15_238 0.44 -0.8 224,048 - HP15_238 0.45 -0.0 224,054 - HP15_238 0.45 +1.2 224,523 - HP15_238 0.87 -0.3
Or see this region's nucleotide sequence