Experiment: benzoic 0.4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiDE and thiC overlap by 1 nucleotides thiC and SO2446 are separated by 435 nucleotides
SO2444: thiDE - phosphomethylpyrimidine kinase/thiamin-phosphate pyrophosphorylase, putative (NCBI ptt file), at 2,556,957 to 2,558,537
thiDE
SO2445: thiC - thiamin biosynthesis protein ThiC (NCBI ptt file), at 2,558,537 to 2,560,702
thiC
SO2446: SO2446 - hypothetical protein (NCBI ptt file), at 2,561,138 to 2,562,205
SO2446
Position (kb)
2558
2559
2560
2561 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2557.552 kb on + strand, within thiDE at 2557.555 kb on - strand, within thiDE at 2557.676 kb on + strand, within thiDE at 2557.690 kb on + strand, within thiDE at 2557.723 kb on + strand, within thiDE at 2557.729 kb on + strand, within thiDE at 2557.742 kb on + strand, within thiDE at 2557.760 kb on - strand, within thiDE at 2557.770 kb on + strand, within thiDE at 2557.778 kb on - strand, within thiDE at 2557.782 kb on + strand, within thiDE at 2557.790 kb on - strand, within thiDE at 2557.794 kb on + strand, within thiDE at 2557.874 kb on + strand, within thiDE at 2557.874 kb on + strand, within thiDE at 2558.275 kb on - strand, within thiDE at 2558.316 kb on - strand, within thiDE at 2558.535 kb on + strand at 2558.543 kb on - strand at 2558.580 kb on + strand at 2558.613 kb on + strand at 2558.615 kb on - strand at 2558.863 kb on - strand, within thiC at 2558.898 kb on - strand, within thiC at 2558.905 kb on - strand, within thiC at 2559.185 kb on + strand, within thiC at 2559.193 kb on - strand, within thiC at 2559.193 kb on - strand, within thiC at 2559.278 kb on - strand, within thiC at 2559.302 kb on - strand, within thiC at 2559.362 kb on + strand, within thiC at 2559.408 kb on + strand, within thiC at 2559.578 kb on + strand, within thiC at 2559.870 kb on - strand, within thiC at 2559.992 kb on - strand, within thiC at 2560.056 kb on - strand, within thiC at 2560.207 kb on - strand, within thiC at 2560.379 kb on - strand, within thiC at 2560.430 kb on - strand, within thiC at 2560.439 kb on + strand, within thiC at 2560.502 kb on - strand at 2560.614 kb on + strand at 2560.687 kb on + strand at 2560.687 kb on + strand at 2560.687 kb on + strand at 2560.687 kb on + strand at 2560.695 kb on - strand at 2560.750 kb on + strand at 2560.829 kb on - strand at 2560.937 kb on + strand at 2560.967 kb on + strand at 2561.166 kb on + strand at 2561.174 kb on - strand at 2561.246 kb on - strand, within SO2446 at 2561.251 kb on - strand, within SO2446 at 2561.343 kb on + strand, within SO2446 at 2561.367 kb on + strand, within SO2446 at 2561.402 kb on + strand, within SO2446 at 2561.553 kb on + strand, within SO2446 at 2561.553 kb on + strand, within SO2446 at 2561.553 kb on + strand, within SO2446 at 2561.553 kb on + strand, within SO2446 at 2561.553 kb on + strand, within SO2446 at 2561.553 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction benzoic 0.4 mg/ml remove 2,557,552 + thiDE SO2444 0.38 +0.6 2,557,555 - thiDE SO2444 0.38 +0.6 2,557,676 + thiDE SO2444 0.45 -0.5 2,557,690 + thiDE SO2444 0.46 -0.0 2,557,723 + thiDE SO2444 0.48 +0.1 2,557,729 + thiDE SO2444 0.49 +0.8 2,557,742 + thiDE SO2444 0.50 +0.5 2,557,760 - thiDE SO2444 0.51 +1.1 2,557,770 + thiDE SO2444 0.51 +0.7 2,557,778 - thiDE SO2444 0.52 -0.5 2,557,782 + thiDE SO2444 0.52 +0.6 2,557,790 - thiDE SO2444 0.53 -0.4 2,557,794 + thiDE SO2444 0.53 +0.4 2,557,874 + thiDE SO2444 0.58 +0.5 2,557,874 + thiDE SO2444 0.58 +0.0 2,558,275 - thiDE SO2444 0.83 +1.0 2,558,316 - thiDE SO2444 0.86 -0.1 2,558,535 + +0.6 2,558,543 - +0.4 2,558,580 + +0.5 2,558,613 + +1.1 2,558,615 - -0.6 2,558,863 - thiC SO2445 0.15 +1.3 2,558,898 - thiC SO2445 0.17 +0.9 2,558,905 - thiC SO2445 0.17 +0.8 2,559,185 + thiC SO2445 0.30 -0.3 2,559,193 - thiC SO2445 0.30 -0.2 2,559,193 - thiC SO2445 0.30 +0.3 2,559,278 - thiC SO2445 0.34 +0.4 2,559,302 - thiC SO2445 0.35 +0.0 2,559,362 + thiC SO2445 0.38 -0.2 2,559,408 + thiC SO2445 0.40 +0.4 2,559,578 + thiC SO2445 0.48 +0.4 2,559,870 - thiC SO2445 0.62 +0.4 2,559,992 - thiC SO2445 0.67 +0.0 2,560,056 - thiC SO2445 0.70 -0.8 2,560,207 - thiC SO2445 0.77 +0.6 2,560,379 - thiC SO2445 0.85 +0.7 2,560,430 - thiC SO2445 0.87 +1.0 2,560,439 + thiC SO2445 0.88 -0.3 2,560,502 - -0.0 2,560,614 + +0.3 2,560,687 + -0.4 2,560,687 + -0.0 2,560,687 + -0.1 2,560,687 + -0.5 2,560,695 - +1.1 2,560,750 + -0.3 2,560,829 - -1.5 2,560,937 + -0.8 2,560,967 + -0.6 2,561,166 + -0.3 2,561,174 - +0.0 2,561,246 - SO2446 0.10 +2.1 2,561,251 - SO2446 0.11 -0.1 2,561,343 + SO2446 0.19 +0.3 2,561,367 + SO2446 0.21 +0.7 2,561,402 + SO2446 0.25 +0.3 2,561,553 + SO2446 0.39 +0.2 2,561,553 + SO2446 0.39 +0.2 2,561,553 + SO2446 0.39 -0.1 2,561,553 + SO2446 0.39 -2.3 2,561,553 + SO2446 0.39 -0.8 2,561,553 + +1.6
Or see this region's nucleotide sequence