Experiment: benzoic 0.4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiS and thiF overlap by 4 nucleotides thiF and thiDE overlap by 4 nucleotides thiDE and thiC overlap by 1 nucleotides
SO2442: thiS - thiS protein, putative (NCBI ptt file), at 2,555,858 to 2,556,061
thiS
SO2443: thiF - thiF protein, putative (NCBI ptt file), at 2,556,058 to 2,556,960
thiF
SO2444: thiDE - phosphomethylpyrimidine kinase/thiamin-phosphate pyrophosphorylase, putative (NCBI ptt file), at 2,556,957 to 2,558,537
thiDE
SO2445: thiC - thiamin biosynthesis protein ThiC (NCBI ptt file), at 2,558,537 to 2,560,702
thiC
Position (kb)
2556
2557
2558
2559 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2556.483 kb on + strand, within thiF at 2556.680 kb on + strand, within thiF at 2556.680 kb on + strand, within thiF at 2556.680 kb on + strand, within thiF at 2556.738 kb on + strand, within thiF at 2556.738 kb on + strand, within thiF at 2556.918 kb on - strand at 2556.971 kb on - strand at 2557.138 kb on - strand, within thiDE at 2557.138 kb on - strand, within thiDE at 2557.214 kb on - strand, within thiDE at 2557.280 kb on + strand, within thiDE at 2557.413 kb on - strand, within thiDE at 2557.483 kb on + strand, within thiDE at 2557.552 kb on + strand, within thiDE at 2557.555 kb on - strand, within thiDE at 2557.676 kb on + strand, within thiDE at 2557.690 kb on + strand, within thiDE at 2557.723 kb on + strand, within thiDE at 2557.729 kb on + strand, within thiDE at 2557.742 kb on + strand, within thiDE at 2557.760 kb on - strand, within thiDE at 2557.770 kb on + strand, within thiDE at 2557.778 kb on - strand, within thiDE at 2557.782 kb on + strand, within thiDE at 2557.790 kb on - strand, within thiDE at 2557.794 kb on + strand, within thiDE at 2557.874 kb on + strand, within thiDE at 2557.874 kb on + strand, within thiDE at 2558.275 kb on - strand, within thiDE at 2558.316 kb on - strand, within thiDE at 2558.535 kb on + strand at 2558.543 kb on - strand at 2558.580 kb on + strand at 2558.613 kb on + strand at 2558.615 kb on - strand at 2558.863 kb on - strand, within thiC at 2558.898 kb on - strand, within thiC at 2558.905 kb on - strand, within thiC at 2559.185 kb on + strand, within thiC at 2559.193 kb on - strand, within thiC at 2559.193 kb on - strand, within thiC at 2559.278 kb on - strand, within thiC at 2559.302 kb on - strand, within thiC at 2559.362 kb on + strand, within thiC at 2559.408 kb on + strand, within thiC
Per-strain Table
Position Strand Gene LocusTag Fraction benzoic 0.4 mg/ml remove 2,556,483 + thiF SO2443 0.47 +0.6 2,556,680 + thiF SO2443 0.69 +0.4 2,556,680 + thiF SO2443 0.69 -0.2 2,556,680 + thiF SO2443 0.69 -2.9 2,556,738 + thiF SO2443 0.75 -0.3 2,556,738 + thiF SO2443 0.75 +0.5 2,556,918 - -0.1 2,556,971 - +0.5 2,557,138 - thiDE SO2444 0.11 +0.2 2,557,138 - thiDE SO2444 0.11 +0.4 2,557,214 - thiDE SO2444 0.16 +0.6 2,557,280 + thiDE SO2444 0.20 +0.4 2,557,413 - thiDE SO2444 0.29 +0.2 2,557,483 + thiDE SO2444 0.33 -1.0 2,557,552 + thiDE SO2444 0.38 +0.6 2,557,555 - thiDE SO2444 0.38 +0.6 2,557,676 + thiDE SO2444 0.45 -0.5 2,557,690 + thiDE SO2444 0.46 -0.0 2,557,723 + thiDE SO2444 0.48 +0.1 2,557,729 + thiDE SO2444 0.49 +0.8 2,557,742 + thiDE SO2444 0.50 +0.5 2,557,760 - thiDE SO2444 0.51 +1.1 2,557,770 + thiDE SO2444 0.51 +0.7 2,557,778 - thiDE SO2444 0.52 -0.5 2,557,782 + thiDE SO2444 0.52 +0.6 2,557,790 - thiDE SO2444 0.53 -0.4 2,557,794 + thiDE SO2444 0.53 +0.4 2,557,874 + thiDE SO2444 0.58 +0.5 2,557,874 + thiDE SO2444 0.58 +0.0 2,558,275 - thiDE SO2444 0.83 +1.0 2,558,316 - thiDE SO2444 0.86 -0.1 2,558,535 + +0.6 2,558,543 - +0.4 2,558,580 + +0.5 2,558,613 + +1.1 2,558,615 - -0.6 2,558,863 - thiC SO2445 0.15 +1.3 2,558,898 - thiC SO2445 0.17 +0.9 2,558,905 - thiC SO2445 0.17 +0.8 2,559,185 + thiC SO2445 0.30 -0.3 2,559,193 - thiC SO2445 0.30 -0.2 2,559,193 - thiC SO2445 0.30 +0.3 2,559,278 - thiC SO2445 0.34 +0.4 2,559,302 - thiC SO2445 0.35 +0.0 2,559,362 + thiC SO2445 0.38 -0.2 2,559,408 + thiC SO2445 0.40 +0.4
Or see this region's nucleotide sequence