Experiment: Aluminum chloride 4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt secD-1 and secF-1 are separated by 2 nucleotides secF-1 and SO1195 are separated by 56 nucleotides SO1195 and rrmJ are separated by 90 nucleotides rrmJ and ftsH are separated by 53 nucleotides
SO1193: secD-1 - protein-export membrane protein SecD (NCBI ptt file), at 1,235,992 to 1,237,848
secD-1
SO1194: secF-1 - protein-export membrane protein SecF (NCBI ptt file), at 1,237,851 to 1,238,735
secF-1
SO1195: SO1195 - conserved hypothetical protein TIGR00253 (NCBI ptt file), at 1,238,792 to 1,239,091
SO1195
SO1196: rrmJ - ribosomal RNA large subunit methyltransferase J (NCBI ptt file), at 1,239,182 to 1,239,811
rrmJ
SO1197: ftsH - cell division protein FtsH (NCBI ptt file), at 1,239,865 to 1,241,814
ftsH
Position (kb)
1238
1239
1240 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1237.849 kb on - strand at 1237.858 kb on + strand at 1237.858 kb on + strand at 1237.906 kb on + strand at 1238.143 kb on + strand, within secF-1 at 1238.151 kb on - strand, within secF-1 at 1238.151 kb on - strand, within secF-1 at 1238.199 kb on - strand, within secF-1 at 1238.227 kb on + strand, within secF-1 at 1238.237 kb on - strand, within secF-1 at 1238.245 kb on - strand, within secF-1 at 1238.402 kb on - strand, within secF-1 at 1238.550 kb on + strand, within secF-1 at 1238.603 kb on - strand, within secF-1 at 1238.611 kb on - strand, within secF-1 at 1238.976 kb on + strand, within SO1195 at 1239.139 kb on - strand at 1239.139 kb on - strand at 1239.141 kb on + strand at 1239.343 kb on - strand, within rrmJ at 1239.660 kb on + strand, within rrmJ at 1239.771 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Aluminum chloride 4 mM remove 1,237,849 - -1.3 1,237,858 + -4.4 1,237,858 + +0.3 1,237,906 + +0.6 1,238,143 + secF-1 SO1194 0.33 +0.0 1,238,151 - secF-1 SO1194 0.34 -0.0 1,238,151 - secF-1 SO1194 0.34 +0.5 1,238,199 - secF-1 SO1194 0.39 +0.3 1,238,227 + secF-1 SO1194 0.42 -0.1 1,238,237 - secF-1 SO1194 0.44 +0.6 1,238,245 - secF-1 SO1194 0.45 +0.1 1,238,402 - secF-1 SO1194 0.62 +0.3 1,238,550 + secF-1 SO1194 0.79 +0.8 1,238,603 - secF-1 SO1194 0.85 +0.3 1,238,611 - secF-1 SO1194 0.86 -1.8 1,238,976 + SO1195 0.61 -0.1 1,239,139 - -1.9 1,239,139 - -0.7 1,239,141 + -2.5 1,239,343 - rrmJ SO1196 0.26 -2.8 1,239,660 + rrmJ SO1196 0.76 -1.1 1,239,771 + -1.9
Or see this region's nucleotide sequence