Experiment: Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt panC and panB are separated by 7 nucleotides panB and Sama_0777 are separated by 13 nucleotides Sama_0777 and Sama_0778 are separated by 2 nucleotides
Sama_0775: panC - pantoate--beta-alanine ligase (RefSeq), at 957,957 to 958,802
panC
Sama_0776: panB - 3-methyl-2-oxobutanoate hydroxymethyltransferase (RefSeq), at 958,810 to 959,649
panB
Sama_0777: Sama_0777 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase (RefSeq), at 959,663 to 960,151
_0777
Sama_0778: Sama_0778 - tRNA nucleotidyltransferase (CCA-adding enzyme) (RefSeq), at 960,154 to 961,428
_0778
Position (kb) 959
960
961 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 958.799 kb on - strand at 958.799 kb on - strand at 958.822 kb on - strand at 958.822 kb on - strand at 958.822 kb on - strand at 958.822 kb on - strand at 958.822 kb on - strand at 959.641 kb on - strand at 959.643 kb on - strand at 959.643 kb on - strand at 959.643 kb on - strand at 959.643 kb on - strand at 959.643 kb on - strand at 960.139 kb on - strand at 960.139 kb on - strand at 960.139 kb on - strand at 960.139 kb on - strand at 960.152 kb on - strand at 960.305 kb on - strand, within Sama_0778 at 960.353 kb on - strand, within Sama_0778 at 960.466 kb on - strand, within Sama_0778 at 960.549 kb on - strand, within Sama_0778 at 960.549 kb on - strand, within Sama_0778 at 960.558 kb on - strand, within Sama_0778 at 960.843 kb on - strand, within Sama_0778 at 960.843 kb on - strand, within Sama_0778 at 960.843 kb on - strand, within Sama_0778 at 960.904 kb on + strand, within Sama_0778 at 960.905 kb on - strand, within Sama_0778 at 960.905 kb on - strand, within Sama_0778 at 961.009 kb on + strand, within Sama_0778 at 961.009 kb on + strand, within Sama_0778 at 961.010 kb on - strand, within Sama_0778 at 961.010 kb on - strand, within Sama_0778 at 961.010 kb on - strand, within Sama_0778 at 961.074 kb on - strand, within Sama_0778 at 961.074 kb on - strand, within Sama_0778 at 961.074 kb on - strand, within Sama_0778 at 961.077 kb on - strand, within Sama_0778 at 961.077 kb on - strand, within Sama_0778 at 961.131 kb on - strand, within Sama_0778
Per-strain Table
Position Strand Gene LocusTag Fraction Putrescine remove 958,799 - -0.9 958,799 - -1.3 958,822 - -2.9 958,822 - -1.1 958,822 - -3.3 958,822 - -1.9 958,822 - -2.6 959,641 - +0.1 959,643 - +0.1 959,643 - +1.2 959,643 - -0.7 959,643 - -0.8 959,643 - -0.0 960,139 - -0.5 960,139 - -1.9 960,139 - -2.6 960,139 - -0.6 960,152 - -2.8 960,305 - Sama_0778 0.12 -0.5 960,353 - Sama_0778 0.16 -0.5 960,466 - Sama_0778 0.24 -0.5 960,549 - Sama_0778 0.31 +0.1 960,549 - Sama_0778 0.31 -0.5 960,558 - Sama_0778 0.32 +0.1 960,843 - Sama_0778 0.54 -2.7 960,843 - Sama_0778 0.54 -1.5 960,843 - Sama_0778 0.54 +0.1 960,904 + Sama_0778 0.59 -0.9 960,905 - Sama_0778 0.59 -1.5 960,905 - Sama_0778 0.59 -2.5 961,009 + Sama_0778 0.67 -2.5 961,009 + Sama_0778 0.67 -0.8 961,010 - Sama_0778 0.67 +0.4 961,010 - Sama_0778 0.67 -1.0 961,010 - Sama_0778 0.67 -2.0 961,074 - Sama_0778 0.72 -2.0 961,074 - Sama_0778 0.72 -1.7 961,074 - Sama_0778 0.72 -2.9 961,077 - Sama_0778 0.72 -1.9 961,077 - Sama_0778 0.72 -1.5 961,131 - Sama_0778 0.77 -2.0
Or see this region's nucleotide sequence