Experiment: Aluminum chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pfs and SO1323 overlap by 2 nucleotides SO1323 and gltD are separated by 62 nucleotides gltD and gltB are separated by 6 nucleotides
SO1322: pfs - 5-methylthioadenosine nucleosidase/S-adenosylhomocysteine nucleosidase (NCBI ptt file), at 1,375,956 to 1,376,666
pfs
SO1323: SO1323 - hypothetical protein (NCBI ptt file), at 1,376,665 to 1,376,871
SO1323
SO1324: gltD - glutamate synthase, small subunit (NCBI ptt file), at 1,376,934 to 1,378,340
gltD
SO1325: gltB - glutamate synthase, large subunit (NCBI ptt file), at 1,378,347 to 1,382,795
gltB
Position (kb)
1376
1377
1378
1379 Strain fitness (log2 ratio)
-2
-1
0
1 at 1376.761 kb on + strand, within SO1323 at 1376.816 kb on - strand, within SO1323 at 1376.823 kb on + strand, within SO1323 at 1376.823 kb on + strand, within SO1323 at 1376.830 kb on - strand, within SO1323 at 1376.873 kb on - strand at 1376.895 kb on + strand at 1376.999 kb on + strand at 1377.008 kb on - strand at 1377.165 kb on - strand, within gltD at 1377.203 kb on - strand, within gltD at 1377.343 kb on - strand, within gltD at 1377.381 kb on - strand, within gltD at 1377.383 kb on + strand, within gltD at 1377.419 kb on - strand, within gltD at 1377.502 kb on - strand, within gltD at 1377.502 kb on - strand, within gltD at 1377.502 kb on - strand, within gltD at 1377.558 kb on + strand, within gltD at 1377.723 kb on + strand, within gltD at 1377.723 kb on + strand, within gltD at 1377.731 kb on - strand, within gltD at 1377.731 kb on - strand, within gltD at 1377.762 kb on - strand, within gltD at 1377.772 kb on - strand, within gltD at 1377.787 kb on - strand, within gltD at 1377.864 kb on + strand, within gltD at 1377.864 kb on + strand, within gltD at 1377.864 kb on + strand, within gltD at 1377.864 kb on + strand, within gltD at 1377.872 kb on - strand, within gltD at 1377.872 kb on - strand, within gltD at 1377.872 kb on - strand, within gltD at 1377.872 kb on - strand, within gltD at 1377.872 kb on - strand, within gltD at 1377.914 kb on + strand, within gltD at 1377.932 kb on - strand, within gltD at 1377.942 kb on + strand, within gltD at 1378.035 kb on - strand, within gltD at 1378.048 kb on + strand, within gltD at 1378.076 kb on + strand, within gltD at 1378.084 kb on - strand, within gltD at 1378.100 kb on - strand, within gltD at 1378.178 kb on - strand, within gltD at 1378.180 kb on + strand, within gltD at 1378.180 kb on + strand, within gltD at 1378.180 kb on + strand, within gltD at 1378.180 kb on + strand, within gltD at 1378.180 kb on + strand, within gltD at 1378.180 kb on + strand, within gltD at 1378.188 kb on - strand, within gltD at 1378.188 kb on - strand, within gltD at 1378.188 kb on - strand, within gltD at 1378.188 kb on - strand, within gltD at 1378.237 kb on + strand at 1378.382 kb on + strand at 1378.390 kb on - strand at 1378.390 kb on - strand at 1378.439 kb on + strand at 1378.719 kb on + strand at 1378.727 kb on - strand at 1378.744 kb on + strand at 1378.770 kb on + strand at 1378.770 kb on + strand at 1378.863 kb on - strand, within gltB at 1378.940 kb on + strand, within gltB at 1378.940 kb on - strand, within gltB at 1378.959 kb on - strand, within gltB at 1378.971 kb on + strand, within gltB at 1378.979 kb on - strand, within gltB at 1379.021 kb on + strand, within gltB at 1379.057 kb on + strand, within gltB at 1379.080 kb on + strand, within gltB
Per-strain Table
Position Strand Gene LocusTag Fraction Aluminum chloride 1 mM remove 1,376,761 + SO1323 0.46 -0.1 1,376,816 - SO1323 0.73 -0.1 1,376,823 + SO1323 0.76 -0.3 1,376,823 + SO1323 0.76 +0.2 1,376,830 - SO1323 0.80 -0.2 1,376,873 - +0.8 1,376,895 + -0.5 1,376,999 + -0.3 1,377,008 - +0.4 1,377,165 - gltD SO1324 0.16 +0.3 1,377,203 - gltD SO1324 0.19 +0.2 1,377,343 - gltD SO1324 0.29 +0.5 1,377,381 - gltD SO1324 0.32 -2.4 1,377,383 + gltD SO1324 0.32 +1.0 1,377,419 - gltD SO1324 0.34 +0.4 1,377,502 - gltD SO1324 0.40 -0.1 1,377,502 - gltD SO1324 0.40 -0.4 1,377,502 - gltD SO1324 0.40 +0.1 1,377,558 + gltD SO1324 0.44 -0.0 1,377,723 + gltD SO1324 0.56 +0.1 1,377,723 + gltD SO1324 0.56 -0.3 1,377,731 - gltD SO1324 0.57 +0.2 1,377,731 - gltD SO1324 0.57 +0.4 1,377,762 - gltD SO1324 0.59 -0.2 1,377,772 - gltD SO1324 0.60 +0.1 1,377,787 - gltD SO1324 0.61 -0.9 1,377,864 + gltD SO1324 0.66 -0.5 1,377,864 + gltD SO1324 0.66 -0.2 1,377,864 + gltD SO1324 0.66 +0.3 1,377,864 + gltD SO1324 0.66 +0.3 1,377,872 - gltD SO1324 0.67 +0.5 1,377,872 - gltD SO1324 0.67 -0.2 1,377,872 - gltD SO1324 0.67 +0.1 1,377,872 - gltD SO1324 0.67 +1.2 1,377,872 - gltD SO1324 0.67 -0.2 1,377,914 + gltD SO1324 0.70 +0.4 1,377,932 - gltD SO1324 0.71 +0.6 1,377,942 + gltD SO1324 0.72 +0.4 1,378,035 - gltD SO1324 0.78 +0.5 1,378,048 + gltD SO1324 0.79 +0.1 1,378,076 + gltD SO1324 0.81 +0.4 1,378,084 - gltD SO1324 0.82 +0.9 1,378,100 - gltD SO1324 0.83 +0.4 1,378,178 - gltD SO1324 0.88 +0.2 1,378,180 + gltD SO1324 0.89 +0.2 1,378,180 + gltD SO1324 0.89 +0.5 1,378,180 + gltD SO1324 0.89 -0.2 1,378,180 + gltD SO1324 0.89 +0.2 1,378,180 + gltD SO1324 0.89 +0.9 1,378,180 + gltD SO1324 0.89 +0.1 1,378,188 - gltD SO1324 0.89 +0.1 1,378,188 - gltD SO1324 0.89 +0.0 1,378,188 - gltD SO1324 0.89 -0.2 1,378,188 - gltD SO1324 0.89 +0.3 1,378,237 + +0.1 1,378,382 + -0.1 1,378,390 - +0.0 1,378,390 - +0.0 1,378,439 + +0.3 1,378,719 + -0.6 1,378,727 - +0.3 1,378,744 + -0.3 1,378,770 + -0.2 1,378,770 + +0.6 1,378,863 - gltB SO1325 0.12 +0.0 1,378,940 + gltB SO1325 0.13 -0.2 1,378,940 - gltB SO1325 0.13 -0.3 1,378,959 - gltB SO1325 0.14 -0.2 1,378,971 + gltB SO1325 0.14 +0.5 1,378,979 - gltB SO1325 0.14 +0.5 1,379,021 + gltB SO1325 0.15 +0.1 1,379,057 + gltB SO1325 0.16 -0.2 1,379,080 + gltB SO1325 0.16 +0.5
Or see this region's nucleotide sequence