Experiment: Adenine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1196 and Psest_1197 are separated by 87 nucleotides Psest_1197 and Psest_1198 are separated by 110 nucleotides Psest_1198 and Psest_1199 are separated by 60 nucleotides Psest_1199 and Psest_1200 overlap by 4 nucleotides
Psest_1196: Psest_1196 - Predicted membrane protein, at 1,299,735 to 1,300,961
_1196
Psest_1197: Psest_1197 - Ureidoglycolate hydrolase, at 1,301,049 to 1,301,561
_1197
Psest_1198: Psest_1198 - allantoicase, at 1,301,672 to 1,302,667
_1198
Psest_1199: Psest_1199 - OHCU decarboxylase, at 1,302,728 to 1,303,243
_1199
Psest_1200: Psest_1200 - putative urate catabolism protein, at 1,303,240 to 1,304,172
_1200
Position (kb)
1301
1302
1303 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1300.711 kb on + strand, within Psest_1196 at 1300.768 kb on + strand, within Psest_1196 at 1300.769 kb on - strand, within Psest_1196 at 1300.783 kb on + strand, within Psest_1196 at 1300.783 kb on + strand, within Psest_1196 at 1300.783 kb on + strand, within Psest_1196 at 1300.783 kb on + strand, within Psest_1196 at 1300.784 kb on - strand, within Psest_1196 at 1300.784 kb on - strand, within Psest_1196 at 1300.873 kb on + strand at 1300.874 kb on - strand at 1300.874 kb on - strand at 1300.874 kb on - strand at 1300.968 kb on + strand at 1300.968 kb on + strand at 1301.009 kb on + strand at 1301.010 kb on - strand at 1301.104 kb on + strand, within Psest_1197 at 1301.104 kb on + strand, within Psest_1197 at 1301.104 kb on + strand, within Psest_1197 at 1301.104 kb on + strand, within Psest_1197 at 1301.105 kb on - strand, within Psest_1197 at 1301.105 kb on - strand, within Psest_1197 at 1301.105 kb on - strand, within Psest_1197 at 1301.203 kb on + strand, within Psest_1197 at 1301.310 kb on + strand, within Psest_1197 at 1301.311 kb on - strand, within Psest_1197 at 1301.311 kb on - strand, within Psest_1197 at 1301.311 kb on - strand, within Psest_1197 at 1301.479 kb on + strand, within Psest_1197 at 1301.479 kb on + strand, within Psest_1197 at 1301.479 kb on + strand, within Psest_1197 at 1301.480 kb on - strand, within Psest_1197 at 1301.630 kb on + strand at 1301.820 kb on - strand, within Psest_1198 at 1301.820 kb on - strand, within Psest_1198 at 1301.878 kb on - strand, within Psest_1198 at 1301.878 kb on - strand, within Psest_1198 at 1301.878 kb on - strand, within Psest_1198 at 1302.348 kb on + strand, within Psest_1198 at 1302.348 kb on + strand, within Psest_1198 at 1302.348 kb on + strand, within Psest_1198 at 1302.349 kb on - strand, within Psest_1198 at 1302.349 kb on - strand, within Psest_1198 at 1302.349 kb on - strand, within Psest_1198 at 1302.349 kb on - strand, within Psest_1198 at 1302.349 kb on - strand, within Psest_1198 at 1302.349 kb on - strand, within Psest_1198 at 1302.460 kb on - strand, within Psest_1198 at 1302.636 kb on + strand at 1302.700 kb on + strand at 1302.700 kb on + strand at 1302.700 kb on + strand at 1302.701 kb on - strand at 1302.701 kb on - strand at 1302.701 kb on - strand at 1302.729 kb on + strand at 1302.729 kb on + strand at 1302.729 kb on + strand at 1302.729 kb on + strand at 1302.729 kb on + strand at 1302.729 kb on + strand at 1302.730 kb on - strand at 1302.894 kb on + strand, within Psest_1199 at 1302.895 kb on - strand, within Psest_1199 at 1302.895 kb on - strand, within Psest_1199 at 1302.895 kb on - strand, within Psest_1199 at 1302.895 kb on - strand, within Psest_1199 at 1303.107 kb on + strand, within Psest_1199 at 1303.170 kb on + strand, within Psest_1199 at 1303.170 kb on + strand, within Psest_1199 at 1303.170 kb on + strand, within Psest_1199 at 1303.443 kb on - strand, within Psest_1200 at 1303.469 kb on + strand, within Psest_1200 at 1303.470 kb on - strand, within Psest_1200 at 1303.541 kb on + strand, within Psest_1200 at 1303.541 kb on + strand, within Psest_1200 at 1303.607 kb on + strand, within Psest_1200 at 1303.607 kb on + strand, within Psest_1200 at 1303.608 kb on - strand, within Psest_1200 at 1303.608 kb on - strand, within Psest_1200 at 1303.623 kb on - strand, within Psest_1200
Per-strain Table
Position Strand Gene LocusTag Fraction Adenine (N) remove 1,300,711 + Psest_1196 0.80 -2.6 1,300,768 + Psest_1196 0.84 -1.0 1,300,769 - Psest_1196 0.84 -1.5 1,300,783 + Psest_1196 0.85 -2.4 1,300,783 + Psest_1196 0.85 -0.1 1,300,783 + Psest_1196 0.85 -2.6 1,300,783 + Psest_1196 0.85 -1.5 1,300,784 - Psest_1196 0.85 -2.6 1,300,784 - Psest_1196 0.85 -0.5 1,300,873 + -1.1 1,300,874 - -1.9 1,300,874 - -0.5 1,300,874 - +0.9 1,300,968 + -0.4 1,300,968 + -1.0 1,301,009 + -2.3 1,301,010 - +0.0 1,301,104 + Psest_1197 0.11 -1.6 1,301,104 + Psest_1197 0.11 +0.1 1,301,104 + Psest_1197 0.11 -1.4 1,301,104 + Psest_1197 0.11 -2.0 1,301,105 - Psest_1197 0.11 -2.8 1,301,105 - Psest_1197 0.11 -1.5 1,301,105 - Psest_1197 0.11 -1.9 1,301,203 + Psest_1197 0.30 -0.8 1,301,310 + Psest_1197 0.51 -1.3 1,301,311 - Psest_1197 0.51 -0.3 1,301,311 - Psest_1197 0.51 +1.1 1,301,311 - Psest_1197 0.51 -0.4 1,301,479 + Psest_1197 0.84 -0.8 1,301,479 + Psest_1197 0.84 +0.1 1,301,479 + Psest_1197 0.84 -2.1 1,301,480 - Psest_1197 0.84 -0.8 1,301,630 + -0.3 1,301,820 - Psest_1198 0.15 +0.2 1,301,820 - Psest_1198 0.15 -0.8 1,301,878 - Psest_1198 0.21 +0.1 1,301,878 - Psest_1198 0.21 +1.2 1,301,878 - Psest_1198 0.21 +0.8 1,302,348 + Psest_1198 0.68 -0.3 1,302,348 + Psest_1198 0.68 -0.5 1,302,348 + Psest_1198 0.68 -0.6 1,302,349 - Psest_1198 0.68 -0.2 1,302,349 - Psest_1198 0.68 +2.6 1,302,349 - Psest_1198 0.68 -1.9 1,302,349 - Psest_1198 0.68 -0.2 1,302,349 - Psest_1198 0.68 -0.0 1,302,349 - Psest_1198 0.68 -0.2 1,302,460 - Psest_1198 0.79 -0.7 1,302,636 + -2.1 1,302,700 + -0.1 1,302,700 + -0.6 1,302,700 + -0.3 1,302,701 - -0.6 1,302,701 - +0.5 1,302,701 - -0.4 1,302,729 + +0.1 1,302,729 + -0.7 1,302,729 + +1.1 1,302,729 + +0.3 1,302,729 + +0.1 1,302,729 + +0.3 1,302,730 - -0.3 1,302,894 + Psest_1199 0.32 -3.3 1,302,895 - Psest_1199 0.32 -3.7 1,302,895 - Psest_1199 0.32 -0.5 1,302,895 - Psest_1199 0.32 -4.9 1,302,895 - Psest_1199 0.32 -2.5 1,303,107 + Psest_1199 0.73 -3.8 1,303,170 + Psest_1199 0.86 -4.2 1,303,170 + Psest_1199 0.86 -2.8 1,303,170 + Psest_1199 0.86 -2.0 1,303,443 - Psest_1200 0.22 -0.9 1,303,469 + Psest_1200 0.25 +0.1 1,303,470 - Psest_1200 0.25 -2.3 1,303,541 + Psest_1200 0.32 -2.3 1,303,541 + Psest_1200 0.32 -3.9 1,303,607 + Psest_1200 0.39 -3.5 1,303,607 + Psest_1200 0.39 -4.1 1,303,608 - Psest_1200 0.39 -0.3 1,303,608 - Psest_1200 0.39 -1.8 1,303,623 - Psest_1200 0.41 -0.5
Or see this region's nucleotide sequence