Experiment: Benzalkonium Chloride 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2306 and lolA are separated by 69 nucleotides lolA and SO2308 are separated by 2 nucleotides SO2308 and crcB overlap by 8 nucleotides crcB and serS are separated by 18 nucleotides
SO2306: SO2306 - cell division protein FtsK, putative (NCBI ptt file), at 2,417,093 to 2,419,828
SO2306
SO2307: lolA - outer membrane lipoprotein carrier protein LolA (NCBI ptt file), at 2,419,898 to 2,420,518
lolA
SO2308: SO2308 - ATPase, AAA family (NCBI ptt file), at 2,420,521 to 2,421,858
SO2308
SO2309: crcB - crcB protein (NCBI ptt file), at 2,421,851 to 2,422,225
crcB
SO2310: serS - seryl-tRNA synthetase (NCBI ptt file), at 2,422,244 to 2,423,530
serS
Position (kb)
2420
2421
2422 Strain fitness (log2 ratio)
-2
-1
0
1 at 2419.526 kb on + strand, within SO2306 at 2420.649 kb on - strand at 2421.046 kb on - strand, within SO2308 at 2421.190 kb on - strand, within SO2308 at 2421.549 kb on + strand, within SO2308 at 2421.612 kb on - strand, within SO2308 at 2421.689 kb on + strand, within SO2308 at 2421.699 kb on + strand, within SO2308 at 2421.826 kb on + strand at 2421.867 kb on - strand at 2421.874 kb on - strand at 2421.877 kb on + strand at 2421.995 kb on + strand, within crcB
Per-strain Table
Position Strand Gene LocusTag Fraction Benzalkonium Chloride 0.002 mg/ml remove 2,419,526 + SO2306 0.89 -1.8 2,420,649 - -0.7 2,421,046 - SO2308 0.39 +0.1 2,421,190 - SO2308 0.50 -0.4 2,421,549 + SO2308 0.77 +0.3 2,421,612 - SO2308 0.82 -0.4 2,421,689 + SO2308 0.87 -1.8 2,421,699 + SO2308 0.88 -0.3 2,421,826 + -0.0 2,421,867 - -0.7 2,421,874 - -0.4 2,421,877 + +0.4 2,421,995 + crcB SO2309 0.38 +1.4
Or see this region's nucleotide sequence