Experiment: marine broth
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_262p01500 and PGA1_262p01510 overlap by 4 nucleotides PGA1_262p01510 and PGA1_262p01520 are separated by 12 nucleotides PGA1_262p01520 and PGA1_262p01530 are separated by 219 nucleotides
PGA1_262p01500: PGA1_262p01500 - sarcosine oxidase subunit alpha, at 163,125 to 166,091
_262p01500
PGA1_262p01510: PGA1_262p01510 - sarcosine oxidase, delta subunit, at 166,088 to 166,396
_262p01510
PGA1_262p01520: PGA1_262p01520 - sarcosine oxidase 3 subunit beta, at 166,409 to 167,659
_262p01520
PGA1_262p01530: PGA1_262p01530 - transcriptional regulator, GntR family, at 167,879 to 169,348
_262p01530
Position (kb) 166
167
168 Strain fitness (log2 ratio) -2
-1
0
1 at 165.429 kb on + strand, within PGA1_262p01500 at 165.690 kb on + strand, within PGA1_262p01500 at 165.690 kb on + strand, within PGA1_262p01500 at 165.698 kb on - strand, within PGA1_262p01500 at 165.698 kb on - strand, within PGA1_262p01500 at 165.905 kb on - strand at 165.952 kb on + strand at 165.952 kb on + strand at 166.158 kb on - strand, within PGA1_262p01510 at 166.200 kb on - strand, within PGA1_262p01510 at 166.200 kb on - strand, within PGA1_262p01510 at 166.283 kb on - strand, within PGA1_262p01510 at 166.402 kb on - strand at 166.554 kb on + strand, within PGA1_262p01520 at 166.554 kb on + strand, within PGA1_262p01520 at 166.758 kb on + strand, within PGA1_262p01520 at 166.924 kb on - strand, within PGA1_262p01520 at 166.947 kb on - strand, within PGA1_262p01520 at 166.972 kb on + strand, within PGA1_262p01520 at 167.015 kb on - strand, within PGA1_262p01520 at 167.104 kb on - strand, within PGA1_262p01520 at 167.216 kb on + strand, within PGA1_262p01520 at 167.476 kb on + strand, within PGA1_262p01520 at 167.513 kb on + strand, within PGA1_262p01520 at 167.520 kb on - strand, within PGA1_262p01520 at 167.628 kb on + strand at 167.628 kb on + strand at 167.628 kb on + strand at 167.628 kb on + strand at 167.631 kb on - strand at 167.636 kb on - strand at 167.704 kb on - strand at 167.729 kb on + strand at 167.737 kb on - strand at 167.750 kb on + strand at 167.758 kb on - strand at 167.897 kb on + strand at 167.974 kb on - strand at 168.106 kb on + strand, within PGA1_262p01530 at 168.274 kb on - strand, within PGA1_262p01530 at 168.276 kb on + strand, within PGA1_262p01530 at 168.333 kb on - strand, within PGA1_262p01530 at 168.596 kb on - strand, within PGA1_262p01530
Per-strain Table
Position Strand Gene LocusTag Fraction marine broth remove 165,429 + PGA1_262p01500 0.78 -1.3 165,690 + PGA1_262p01500 0.86 -1.2 165,690 + PGA1_262p01500 0.86 -0.5 165,698 - PGA1_262p01500 0.87 -0.7 165,698 - PGA1_262p01500 0.87 -0.1 165,905 - +0.4 165,952 + +0.6 165,952 + +0.6 166,158 - PGA1_262p01510 0.23 +1.4 166,200 - PGA1_262p01510 0.36 +0.2 166,200 - PGA1_262p01510 0.36 -0.6 166,283 - PGA1_262p01510 0.63 +0.5 166,402 - -0.3 166,554 + PGA1_262p01520 0.12 -0.9 166,554 + PGA1_262p01520 0.12 -0.8 166,758 + PGA1_262p01520 0.28 -0.2 166,924 - PGA1_262p01520 0.41 -0.2 166,947 - PGA1_262p01520 0.43 +1.2 166,972 + PGA1_262p01520 0.45 -2.4 167,015 - PGA1_262p01520 0.48 -0.7 167,104 - PGA1_262p01520 0.56 -0.3 167,216 + PGA1_262p01520 0.65 -0.3 167,476 + PGA1_262p01520 0.85 -1.3 167,513 + PGA1_262p01520 0.88 -0.7 167,520 - PGA1_262p01520 0.89 -0.8 167,628 + +0.3 167,628 + +1.0 167,628 + -0.1 167,628 + +0.9 167,631 - -0.4 167,636 - -0.3 167,704 - +1.1 167,729 + +0.3 167,737 - -0.7 167,750 + -1.8 167,758 - -0.2 167,897 + +0.2 167,974 - -0.4 168,106 + PGA1_262p01530 0.15 -0.1 168,274 - PGA1_262p01530 0.27 +0.8 168,276 + PGA1_262p01530 0.27 +0.6 168,333 - PGA1_262p01530 0.31 -1.1 168,596 - PGA1_262p01530 0.49 +1.1
Or see this region's nucleotide sequence