Experiment: Putrescine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2024 and Psest_2025 are separated by 61 nucleotides Psest_2025 and Psest_2026 are separated by 218 nucleotides Psest_2026 and Psest_2027 are separated by 29 nucleotides
Psest_2024: Psest_2024 - isocitrate dehydrogenase, NADP-dependent, prokaryotic type, at 2,144,486 to 2,145,742
_2024
Psest_2025: Psest_2025 - cold shock domain protein CspD, at 2,145,804 to 2,146,064
_2025
Psest_2026: Psest_2026 - Uncharacterized conserved protein, at 2,146,283 to 2,146,645
_2026
Psest_2027: Psest_2027 - ATP-dependent Clp protease ATP-binding subunit clpA, at 2,146,675 to 2,148,945
_2027
Position (kb)
2146
2147 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2145.313 kb on + strand, within Psest_2024 at 2145.413 kb on + strand, within Psest_2024 at 2145.413 kb on + strand, within Psest_2024 at 2145.413 kb on + strand, within Psest_2024 at 2145.414 kb on - strand, within Psest_2024 at 2145.414 kb on - strand, within Psest_2024 at 2145.538 kb on + strand, within Psest_2024 at 2145.538 kb on + strand, within Psest_2024 at 2145.538 kb on + strand, within Psest_2024 at 2145.538 kb on + strand, within Psest_2024 at 2145.538 kb on + strand, within Psest_2024 at 2145.539 kb on - strand, within Psest_2024 at 2145.539 kb on - strand, within Psest_2024 at 2145.539 kb on - strand, within Psest_2024 at 2145.600 kb on + strand, within Psest_2024 at 2145.601 kb on - strand, within Psest_2024 at 2145.662 kb on + strand at 2145.663 kb on - strand at 2145.736 kb on + strand at 2145.736 kb on + strand at 2145.736 kb on + strand at 2145.736 kb on + strand at 2145.736 kb on + strand at 2145.736 kb on + strand at 2145.736 kb on + strand at 2145.736 kb on + strand at 2145.737 kb on - strand at 2145.737 kb on - strand at 2145.737 kb on - strand at 2145.737 kb on - strand at 2145.737 kb on - strand at 2145.737 kb on - strand at 2145.740 kb on + strand at 2145.741 kb on - strand at 2146.008 kb on + strand, within Psest_2025 at 2146.059 kb on + strand at 2146.059 kb on + strand at 2146.060 kb on - strand at 2146.060 kb on - strand at 2146.101 kb on + strand at 2146.101 kb on + strand at 2146.101 kb on + strand at 2146.124 kb on + strand at 2146.167 kb on + strand at 2146.168 kb on - strand at 2146.175 kb on + strand at 2146.175 kb on + strand at 2146.176 kb on - strand at 2146.176 kb on - strand at 2146.184 kb on + strand at 2146.185 kb on - strand at 2146.241 kb on - strand at 2146.245 kb on - strand at 2146.245 kb on - strand at 2146.245 kb on - strand at 2146.245 kb on - strand at 2146.268 kb on - strand at 2146.308 kb on + strand at 2146.308 kb on + strand at 2146.308 kb on + strand at 2146.403 kb on + strand, within Psest_2026 at 2146.404 kb on - strand, within Psest_2026 at 2146.404 kb on - strand, within Psest_2026 at 2146.404 kb on - strand, within Psest_2026 at 2146.404 kb on - strand, within Psest_2026 at 2146.404 kb on - strand, within Psest_2026 at 2146.404 kb on - strand, within Psest_2026 at 2146.405 kb on + strand, within Psest_2026 at 2146.405 kb on + strand, within Psest_2026 at 2146.405 kb on + strand, within Psest_2026 at 2146.405 kb on + strand, within Psest_2026 at 2146.405 kb on + strand, within Psest_2026 at 2146.405 kb on + strand, within Psest_2026 at 2146.405 kb on + strand, within Psest_2026 at 2146.406 kb on - strand, within Psest_2026 at 2146.406 kb on - strand, within Psest_2026 at 2146.406 kb on - strand, within Psest_2026 at 2146.406 kb on - strand, within Psest_2026 at 2146.406 kb on - strand, within Psest_2026 at 2146.406 kb on - strand, within Psest_2026 at 2146.406 kb on - strand, within Psest_2026 at 2146.406 kb on - strand, within Psest_2026 at 2146.406 kb on - strand, within Psest_2026 at 2146.420 kb on + strand, within Psest_2026 at 2146.421 kb on - strand, within Psest_2026 at 2146.431 kb on - strand, within Psest_2026 at 2146.547 kb on + strand, within Psest_2026 at 2146.547 kb on + strand, within Psest_2026 at 2146.548 kb on - strand, within Psest_2026 at 2146.592 kb on + strand, within Psest_2026 at 2146.592 kb on + strand, within Psest_2026 at 2146.593 kb on - strand, within Psest_2026 at 2146.674 kb on + strand at 2146.674 kb on + strand at 2146.674 kb on + strand at 2146.675 kb on - strand at 2146.675 kb on - strand at 2146.675 kb on - strand at 2146.675 kb on - strand at 2146.675 kb on - strand at 2146.675 kb on - strand at 2146.728 kb on + strand at 2146.728 kb on + strand at 2146.879 kb on - strand at 2147.083 kb on + strand, within Psest_2027 at 2147.084 kb on - strand, within Psest_2027 at 2147.084 kb on - strand, within Psest_2027 at 2147.084 kb on - strand, within Psest_2027 at 2147.084 kb on - strand, within Psest_2027 at 2147.084 kb on - strand, within Psest_2027 at 2147.084 kb on - strand, within Psest_2027 at 2147.200 kb on + strand, within Psest_2027 at 2147.200 kb on + strand, within Psest_2027 at 2147.200 kb on + strand, within Psest_2027 at 2147.201 kb on - strand, within Psest_2027 at 2147.201 kb on - strand, within Psest_2027 at 2147.201 kb on - strand, within Psest_2027 at 2147.253 kb on - strand, within Psest_2027 at 2147.423 kb on - strand, within Psest_2027 at 2147.447 kb on + strand, within Psest_2027 at 2147.454 kb on + strand, within Psest_2027 at 2147.550 kb on + strand, within Psest_2027 at 2147.603 kb on + strand, within Psest_2027
Per-strain Table
Position Strand Gene LocusTag Fraction Putrescine (N) remove 2,145,313 + Psest_2024 0.66 +1.3 2,145,413 + Psest_2024 0.74 +0.9 2,145,413 + Psest_2024 0.74 +0.1 2,145,413 + Psest_2024 0.74 +0.6 2,145,414 - Psest_2024 0.74 +0.2 2,145,414 - Psest_2024 0.74 -0.9 2,145,538 + Psest_2024 0.84 +0.7 2,145,538 + Psest_2024 0.84 +0.9 2,145,538 + Psest_2024 0.84 -1.2 2,145,538 + Psest_2024 0.84 +1.7 2,145,538 + Psest_2024 0.84 +0.9 2,145,539 - Psest_2024 0.84 +0.5 2,145,539 - Psest_2024 0.84 -0.4 2,145,539 - Psest_2024 0.84 +0.2 2,145,600 + Psest_2024 0.89 +0.5 2,145,601 - Psest_2024 0.89 +1.0 2,145,662 + +0.3 2,145,663 - +0.2 2,145,736 + -2.0 2,145,736 + +0.5 2,145,736 + -0.6 2,145,736 + -2.1 2,145,736 + +0.7 2,145,736 + -1.2 2,145,736 + -2.5 2,145,736 + -0.5 2,145,737 - +1.0 2,145,737 - -0.7 2,145,737 - +0.1 2,145,737 - -0.4 2,145,737 - -1.1 2,145,737 - -1.1 2,145,740 + +0.9 2,145,741 - -0.8 2,146,008 + Psest_2025 0.78 +0.3 2,146,059 + +0.0 2,146,059 + +0.2 2,146,060 - +0.7 2,146,060 - +0.5 2,146,101 + +0.3 2,146,101 + +0.8 2,146,101 + -1.5 2,146,124 + +0.7 2,146,167 + +0.6 2,146,168 - +0.4 2,146,175 + +1.4 2,146,175 + +0.6 2,146,176 - -0.4 2,146,176 - -0.3 2,146,184 + -0.5 2,146,185 - -2.0 2,146,241 - -1.5 2,146,245 - +0.6 2,146,245 - -2.1 2,146,245 - -1.9 2,146,245 - -2.3 2,146,268 - -1.1 2,146,308 + +1.9 2,146,308 + +0.2 2,146,308 + +0.5 2,146,403 + Psest_2026 0.33 +0.3 2,146,404 - Psest_2026 0.33 -1.5 2,146,404 - Psest_2026 0.33 -0.2 2,146,404 - Psest_2026 0.33 -0.9 2,146,404 - Psest_2026 0.33 -1.5 2,146,404 - Psest_2026 0.33 -1.3 2,146,404 - Psest_2026 0.33 +0.2 2,146,405 + Psest_2026 0.34 +0.8 2,146,405 + Psest_2026 0.34 +0.0 2,146,405 + Psest_2026 0.34 -0.2 2,146,405 + Psest_2026 0.34 -0.3 2,146,405 + Psest_2026 0.34 +0.5 2,146,405 + Psest_2026 0.34 +0.9 2,146,405 + Psest_2026 0.34 +0.2 2,146,406 - Psest_2026 0.34 -2.3 2,146,406 - Psest_2026 0.34 -1.1 2,146,406 - Psest_2026 0.34 +0.3 2,146,406 - Psest_2026 0.34 -1.2 2,146,406 - Psest_2026 0.34 -0.4 2,146,406 - Psest_2026 0.34 -0.4 2,146,406 - Psest_2026 0.34 -1.1 2,146,406 - Psest_2026 0.34 -2.5 2,146,406 - Psest_2026 0.34 -0.9 2,146,420 + Psest_2026 0.38 -0.3 2,146,421 - Psest_2026 0.38 -0.7 2,146,431 - Psest_2026 0.41 -0.7 2,146,547 + Psest_2026 0.73 -0.2 2,146,547 + Psest_2026 0.73 +0.1 2,146,548 - Psest_2026 0.73 -1.0 2,146,592 + Psest_2026 0.85 +0.6 2,146,592 + Psest_2026 0.85 +0.2 2,146,593 - Psest_2026 0.85 -1.8 2,146,674 + +0.7 2,146,674 + -0.4 2,146,674 + +0.0 2,146,675 - -1.4 2,146,675 - -0.8 2,146,675 - -3.3 2,146,675 - -1.4 2,146,675 - -0.9 2,146,675 - -0.7 2,146,728 + -0.1 2,146,728 + +0.2 2,146,879 - -1.6 2,147,083 + Psest_2027 0.18 -1.9 2,147,084 - Psest_2027 0.18 -2.2 2,147,084 - Psest_2027 0.18 -1.0 2,147,084 - Psest_2027 0.18 -2.9 2,147,084 - Psest_2027 0.18 -1.2 2,147,084 - Psest_2027 0.18 -2.0 2,147,084 - Psest_2027 0.18 -1.5 2,147,200 + Psest_2027 0.23 -0.9 2,147,200 + Psest_2027 0.23 -0.5 2,147,200 + Psest_2027 0.23 -0.2 2,147,201 - Psest_2027 0.23 -2.5 2,147,201 - Psest_2027 0.23 -0.6 2,147,201 - Psest_2027 0.23 -2.2 2,147,253 - Psest_2027 0.25 -1.1 2,147,423 - Psest_2027 0.33 +1.1 2,147,447 + Psest_2027 0.34 -1.5 2,147,454 + Psest_2027 0.34 -0.4 2,147,550 + Psest_2027 0.39 +0.1 2,147,603 + Psest_2027 0.41 -1.0
Or see this region's nucleotide sequence