Experiment: m.b. Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c09230 and PGA1_c09240 overlap by 4 nucleotides PGA1_c09240 and PGA1_c09250 are separated by 34 nucleotides PGA1_c09250 and PGA1_c09260 are separated by 225 nucleotides PGA1_c09260 and PGA1_c09270 are separated by 144 nucleotides
PGA1_c09230: PGA1_c09230 - phosphoribosyl-ATP pyrophosphatase HisE, at 946,905 to 947,222
_c09230
PGA1_c09240: PGA1_c09240 - imidazole glycerol phosphate synthase subunit HisF, at 947,219 to 947,980
_c09240
PGA1_c09250: PGA1_c09250 - Protein of unknown function (DUF2867)., at 948,015 to 948,497
_c09250
PGA1_c09260: PGA1_c09260 - Uncharacterized conserved protein, at 948,723 to 949,169
_c09260
PGA1_c09270: PGA1_c09270 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase HisA, at 949,314 to 950,036
_c09270
Position (kb) 948
949 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 948.010 kb on + strand at 948.020 kb on - strand at 948.054 kb on - strand at 948.054 kb on - strand at 948.093 kb on - strand, within PGA1_c09250 at 948.115 kb on + strand, within PGA1_c09250 at 948.160 kb on + strand, within PGA1_c09250 at 948.227 kb on + strand, within PGA1_c09250 at 948.235 kb on - strand, within PGA1_c09250 at 948.235 kb on - strand, within PGA1_c09250 at 948.245 kb on + strand, within PGA1_c09250 at 948.245 kb on + strand, within PGA1_c09250 at 948.252 kb on + strand, within PGA1_c09250 at 948.252 kb on + strand, within PGA1_c09250 at 948.253 kb on - strand, within PGA1_c09250 at 948.344 kb on - strand, within PGA1_c09250 at 948.436 kb on + strand, within PGA1_c09250 at 948.510 kb on + strand at 948.516 kb on - strand at 948.523 kb on - strand at 948.532 kb on + strand at 948.532 kb on + strand at 948.540 kb on - strand at 948.551 kb on - strand at 948.593 kb on + strand at 948.616 kb on + strand at 948.618 kb on - strand at 948.618 kb on - strand at 948.624 kb on - strand at 948.635 kb on + strand at 948.662 kb on + strand at 948.669 kb on + strand at 948.669 kb on + strand at 948.669 kb on + strand at 948.669 kb on + strand at 948.674 kb on + strand at 948.674 kb on + strand at 948.674 kb on + strand at 948.674 kb on + strand at 948.677 kb on - strand at 948.677 kb on - strand at 948.677 kb on - strand at 948.677 kb on - strand at 948.679 kb on + strand at 948.679 kb on + strand at 948.682 kb on - strand at 948.682 kb on - strand at 948.682 kb on - strand at 948.707 kb on + strand at 948.759 kb on + strand at 948.781 kb on + strand, within PGA1_c09260 at 948.781 kb on + strand, within PGA1_c09260 at 948.845 kb on - strand, within PGA1_c09260 at 948.870 kb on + strand, within PGA1_c09260 at 948.870 kb on + strand, within PGA1_c09260 at 948.975 kb on - strand, within PGA1_c09260 at 948.975 kb on - strand, within PGA1_c09260 at 949.091 kb on + strand, within PGA1_c09260 at 949.153 kb on - strand at 949.235 kb on - strand at 949.282 kb on + strand at 949.285 kb on - strand at 949.290 kb on - strand at 949.329 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Doxycycline hyclate 0.001 mg/ml remove 948,010 + -0.5 948,020 - +2.1 948,054 - +0.8 948,054 - +1.4 948,093 - PGA1_c09250 0.16 -0.8 948,115 + PGA1_c09250 0.21 +1.0 948,160 + PGA1_c09250 0.30 -0.5 948,227 + PGA1_c09250 0.44 +1.8 948,235 - PGA1_c09250 0.46 -3.0 948,235 - PGA1_c09250 0.46 +0.5 948,245 + PGA1_c09250 0.48 +1.4 948,245 + PGA1_c09250 0.48 -1.5 948,252 + PGA1_c09250 0.49 -0.5 948,252 + PGA1_c09250 0.49 +0.4 948,253 - PGA1_c09250 0.49 +0.9 948,344 - PGA1_c09250 0.68 +0.6 948,436 + PGA1_c09250 0.87 -0.2 948,510 + -1.0 948,516 - -0.4 948,523 - +1.4 948,532 + -0.8 948,532 + +1.0 948,540 - -0.4 948,551 - +0.0 948,593 + -1.3 948,616 + -0.3 948,618 - -2.8 948,618 - -0.2 948,624 - +0.5 948,635 + +2.2 948,662 + +1.0 948,669 + +1.0 948,669 + +2.2 948,669 + +0.8 948,669 + +0.3 948,674 + +1.2 948,674 + -1.1 948,674 + -1.0 948,674 + -0.1 948,677 - -1.3 948,677 - -0.5 948,677 - +1.2 948,677 - +0.8 948,679 + -0.5 948,679 + +1.2 948,682 - -0.7 948,682 - +0.4 948,682 - -0.2 948,707 + -0.8 948,759 + -1.5 948,781 + PGA1_c09260 0.13 -0.5 948,781 + PGA1_c09260 0.13 -0.9 948,845 - PGA1_c09260 0.27 -0.2 948,870 + PGA1_c09260 0.33 -0.4 948,870 + PGA1_c09260 0.33 +1.0 948,975 - PGA1_c09260 0.56 -1.0 948,975 - PGA1_c09260 0.56 +1.8 949,091 + PGA1_c09260 0.82 -1.0 949,153 - -0.0 949,235 - -0.4 949,282 + +1.1 949,285 - +1.1 949,290 - -0.4 949,329 + -0.4
Or see this region's nucleotide sequence