Experiment: m.b. Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c05610 and PGA1_c05620 overlap by 4 nucleotides PGA1_c05620 and PGA1_c05630 are separated by 25 nucleotides PGA1_c05630 and PGA1_c05640 are separated by 37 nucleotides PGA1_c05640 and PGA1_c05650 are separated by 155 nucleotides
PGA1_c05610: PGA1_c05610 - putative queuosine biosynthesis protein QueD, at 574,896 to 575,249
_c05610
PGA1_c05620: PGA1_c05620 - putative 7-cyano-7-deazaguanosine biosynthesis protein QueE, at 575,246 to 576,043
_c05620
PGA1_c05630: PGA1_c05630 - NADPH-dependent 7-cyano-7-deazaguanine reductase QueF, at 576,069 to 576,533
_c05630
PGA1_c05640: PGA1_c05640 - hypothetical protein, at 576,571 to 577,242
_c05640
PGA1_c05650: PGA1_c05650 - tRNA pseudouridine synthase A, at 577,398 to 578,171
_c05650
Position (kb)
576
577 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 575.106 kb on + strand, within PGA1_c05610 at 575.124 kb on - strand, within PGA1_c05610 at 575.139 kb on + strand, within PGA1_c05610 at 575.139 kb on + strand, within PGA1_c05610 at 575.139 kb on + strand, within PGA1_c05610 at 575.147 kb on - strand, within PGA1_c05610 at 575.147 kb on - strand, within PGA1_c05610 at 575.187 kb on + strand, within PGA1_c05610 at 575.237 kb on + strand at 575.322 kb on + strand at 575.377 kb on + strand, within PGA1_c05620 at 575.408 kb on + strand, within PGA1_c05620 at 575.432 kb on + strand, within PGA1_c05620 at 575.433 kb on - strand, within PGA1_c05620 at 575.465 kb on - strand, within PGA1_c05620 at 575.465 kb on - strand, within PGA1_c05620 at 575.520 kb on + strand, within PGA1_c05620 at 575.520 kb on + strand, within PGA1_c05620 at 575.525 kb on + strand, within PGA1_c05620 at 575.617 kb on + strand, within PGA1_c05620 at 575.617 kb on + strand, within PGA1_c05620 at 575.617 kb on + strand, within PGA1_c05620 at 575.651 kb on + strand, within PGA1_c05620 at 575.671 kb on + strand, within PGA1_c05620 at 575.684 kb on - strand, within PGA1_c05620 at 575.702 kb on - strand, within PGA1_c05620 at 575.761 kb on + strand, within PGA1_c05620 at 575.761 kb on + strand, within PGA1_c05620 at 575.779 kb on - strand, within PGA1_c05620 at 575.822 kb on + strand, within PGA1_c05620 at 575.884 kb on + strand, within PGA1_c05620 at 575.890 kb on + strand, within PGA1_c05620 at 575.979 kb on + strand at 575.987 kb on - strand at 576.016 kb on + strand at 576.070 kb on + strand at 576.101 kb on + strand at 576.109 kb on + strand at 576.117 kb on - strand, within PGA1_c05630 at 576.117 kb on - strand, within PGA1_c05630 at 576.117 kb on - strand, within PGA1_c05630 at 576.117 kb on - strand, within PGA1_c05630 at 576.117 kb on - strand, within PGA1_c05630 at 576.117 kb on - strand, within PGA1_c05630 at 576.122 kb on - strand, within PGA1_c05630 at 576.122 kb on - strand, within PGA1_c05630 at 576.138 kb on - strand, within PGA1_c05630 at 576.148 kb on - strand, within PGA1_c05630 at 576.174 kb on + strand, within PGA1_c05630 at 576.226 kb on - strand, within PGA1_c05630 at 576.226 kb on - strand, within PGA1_c05630 at 576.239 kb on + strand, within PGA1_c05630 at 576.350 kb on + strand, within PGA1_c05630 at 576.466 kb on + strand, within PGA1_c05630 at 576.474 kb on - strand, within PGA1_c05630 at 576.476 kb on + strand, within PGA1_c05630 at 576.484 kb on - strand, within PGA1_c05630 at 576.484 kb on - strand, within PGA1_c05630 at 576.506 kb on - strand at 576.506 kb on - strand at 576.512 kb on + strand at 576.538 kb on + strand at 576.676 kb on + strand, within PGA1_c05640 at 576.714 kb on + strand, within PGA1_c05640 at 576.748 kb on + strand, within PGA1_c05640 at 576.756 kb on - strand, within PGA1_c05640 at 576.756 kb on - strand, within PGA1_c05640 at 576.865 kb on + strand, within PGA1_c05640 at 576.865 kb on + strand, within PGA1_c05640 at 576.873 kb on - strand, within PGA1_c05640 at 576.873 kb on - strand, within PGA1_c05640 at 576.873 kb on - strand, within PGA1_c05640 at 576.873 kb on - strand, within PGA1_c05640 at 577.048 kb on - strand, within PGA1_c05640 at 577.121 kb on - strand, within PGA1_c05640 at 577.121 kb on - strand, within PGA1_c05640 at 577.349 kb on + strand at 577.357 kb on - strand at 577.357 kb on - strand at 577.357 kb on - strand at 577.359 kb on + strand at 577.367 kb on - strand at 577.374 kb on + strand at 577.467 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Doxycycline hyclate 0.001 mg/ml remove 575,106 + PGA1_c05610 0.59 +1.2 575,124 - PGA1_c05610 0.64 +1.9 575,139 + PGA1_c05610 0.69 +0.9 575,139 + PGA1_c05610 0.69 +2.3 575,139 + PGA1_c05610 0.69 -0.1 575,147 - PGA1_c05610 0.71 +1.6 575,147 - PGA1_c05610 0.71 +1.2 575,187 + PGA1_c05610 0.82 +2.1 575,237 + +0.1 575,322 + -0.1 575,377 + PGA1_c05620 0.16 -0.1 575,408 + PGA1_c05620 0.20 -1.1 575,432 + PGA1_c05620 0.23 +2.5 575,433 - PGA1_c05620 0.23 +1.9 575,465 - PGA1_c05620 0.27 +0.8 575,465 - PGA1_c05620 0.27 +0.1 575,520 + PGA1_c05620 0.34 -2.2 575,520 + PGA1_c05620 0.34 -0.6 575,525 + PGA1_c05620 0.35 -0.2 575,617 + PGA1_c05620 0.46 -0.8 575,617 + PGA1_c05620 0.46 +2.5 575,617 + PGA1_c05620 0.46 +1.9 575,651 + PGA1_c05620 0.51 +0.2 575,671 + PGA1_c05620 0.53 +1.4 575,684 - PGA1_c05620 0.55 -0.1 575,702 - PGA1_c05620 0.57 +1.4 575,761 + PGA1_c05620 0.65 +1.9 575,761 + PGA1_c05620 0.65 +0.5 575,779 - PGA1_c05620 0.67 -0.6 575,822 + PGA1_c05620 0.72 -0.2 575,884 + PGA1_c05620 0.80 -1.4 575,890 + PGA1_c05620 0.81 -0.6 575,979 + +0.2 575,987 - +2.1 576,016 + -1.6 576,070 + +0.4 576,101 + -0.1 576,109 + -1.0 576,117 - PGA1_c05630 0.10 +0.1 576,117 - PGA1_c05630 0.10 +0.2 576,117 - PGA1_c05630 0.10 +0.4 576,117 - PGA1_c05630 0.10 -0.0 576,117 - PGA1_c05630 0.10 +0.5 576,117 - PGA1_c05630 0.10 +0.5 576,122 - PGA1_c05630 0.11 +0.7 576,122 - PGA1_c05630 0.11 +1.8 576,138 - PGA1_c05630 0.15 +1.4 576,148 - PGA1_c05630 0.17 +1.0 576,174 + PGA1_c05630 0.23 +1.3 576,226 - PGA1_c05630 0.34 -1.6 576,226 - PGA1_c05630 0.34 -0.0 576,239 + PGA1_c05630 0.37 -0.0 576,350 + PGA1_c05630 0.60 +1.2 576,466 + PGA1_c05630 0.85 +1.1 576,474 - PGA1_c05630 0.87 -0.3 576,476 + PGA1_c05630 0.88 +0.1 576,484 - PGA1_c05630 0.89 +1.4 576,484 - PGA1_c05630 0.89 +0.1 576,506 - +0.5 576,506 - +2.5 576,512 + +0.4 576,538 + +1.0 576,676 + PGA1_c05640 0.16 +1.3 576,714 + PGA1_c05640 0.21 +2.1 576,748 + PGA1_c05640 0.26 +1.0 576,756 - PGA1_c05640 0.28 -0.8 576,756 - PGA1_c05640 0.28 +0.4 576,865 + PGA1_c05640 0.44 +2.0 576,865 + PGA1_c05640 0.44 +0.4 576,873 - PGA1_c05640 0.45 +1.2 576,873 - PGA1_c05640 0.45 +0.3 576,873 - PGA1_c05640 0.45 +0.6 576,873 - PGA1_c05640 0.45 +0.4 577,048 - PGA1_c05640 0.71 -0.5 577,121 - PGA1_c05640 0.82 +1.5 577,121 - PGA1_c05640 0.82 +2.2 577,349 + +0.7 577,357 - +0.5 577,357 - +2.2 577,357 - +1.4 577,359 + +1.5 577,367 - -0.0 577,374 + -1.0 577,467 - -1.0
Or see this region's nucleotide sequence