Experiment: m.b. Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c05110 and PGA1_c05120 overlap by 10 nucleotides PGA1_c05120 and PGA1_c05130 overlap by 1 nucleotides PGA1_c05130 and PGA1_c05140 are separated by 53 nucleotides
PGA1_c05110: PGA1_c05110 - dihydrodipicolinate synthase domain-containing protein, at 503,564 to 504,523
_c05110
PGA1_c05120: PGA1_c05120 - 4-hydroxyproline epimerase, at 504,514 to 505,458
_c05120
PGA1_c05130: PGA1_c05130 - aldehyde dehydrogenase, at 505,458 to 506,888
_c05130
PGA1_c05140: PGA1_c05140 - D-amino acid dehydrogenase small subunit, at 506,942 to 508,192
_c05140
Position (kb)
505
506
507 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 504.471 kb on - strand at 504.474 kb on + strand at 504.474 kb on + strand at 504.482 kb on + strand at 504.490 kb on - strand at 504.490 kb on - strand at 504.554 kb on + strand at 504.562 kb on - strand at 504.644 kb on + strand, within PGA1_c05120 at 504.652 kb on - strand, within PGA1_c05120 at 504.652 kb on - strand, within PGA1_c05120 at 504.717 kb on - strand, within PGA1_c05120 at 504.787 kb on - strand, within PGA1_c05120 at 504.805 kb on + strand, within PGA1_c05120 at 504.813 kb on - strand, within PGA1_c05120 at 504.815 kb on + strand, within PGA1_c05120 at 504.857 kb on + strand, within PGA1_c05120 at 504.983 kb on - strand, within PGA1_c05120 at 505.004 kb on + strand, within PGA1_c05120 at 505.010 kb on - strand, within PGA1_c05120 at 505.012 kb on - strand, within PGA1_c05120 at 505.022 kb on + strand, within PGA1_c05120 at 505.029 kb on - strand, within PGA1_c05120 at 505.032 kb on + strand, within PGA1_c05120 at 505.073 kb on + strand, within PGA1_c05120 at 505.075 kb on - strand, within PGA1_c05120 at 505.078 kb on + strand, within PGA1_c05120 at 505.085 kb on - strand, within PGA1_c05120 at 505.085 kb on - strand, within PGA1_c05120 at 505.101 kb on - strand, within PGA1_c05120 at 505.118 kb on + strand, within PGA1_c05120 at 505.142 kb on + strand, within PGA1_c05120 at 505.147 kb on + strand, within PGA1_c05120 at 505.147 kb on + strand, within PGA1_c05120 at 505.147 kb on + strand, within PGA1_c05120 at 505.147 kb on + strand, within PGA1_c05120 at 505.150 kb on - strand, within PGA1_c05120 at 505.155 kb on - strand, within PGA1_c05120 at 505.155 kb on - strand, within PGA1_c05120 at 505.155 kb on - strand, within PGA1_c05120 at 505.155 kb on - strand, within PGA1_c05120 at 505.155 kb on - strand, within PGA1_c05120 at 505.155 kb on - strand, within PGA1_c05120 at 505.155 kb on - strand, within PGA1_c05120 at 505.198 kb on + strand, within PGA1_c05120 at 505.198 kb on + strand, within PGA1_c05120 at 505.206 kb on - strand, within PGA1_c05120 at 505.206 kb on - strand, within PGA1_c05120 at 505.206 kb on - strand, within PGA1_c05120 at 505.206 kb on - strand, within PGA1_c05120 at 505.206 kb on - strand, within PGA1_c05120 at 505.286 kb on + strand, within PGA1_c05120 at 505.286 kb on + strand, within PGA1_c05120 at 505.286 kb on + strand, within PGA1_c05120 at 505.313 kb on + strand, within PGA1_c05120 at 505.331 kb on + strand, within PGA1_c05120 at 505.420 kb on - strand at 505.442 kb on + strand at 505.447 kb on + strand at 505.450 kb on - strand at 505.497 kb on - strand at 505.497 kb on - strand at 505.498 kb on - strand at 505.550 kb on - strand at 505.611 kb on + strand, within PGA1_c05130 at 505.613 kb on + strand, within PGA1_c05130 at 505.619 kb on - strand, within PGA1_c05130 at 505.619 kb on - strand, within PGA1_c05130 at 505.645 kb on + strand, within PGA1_c05130 at 505.655 kb on + strand, within PGA1_c05130 at 505.698 kb on - strand, within PGA1_c05130 at 505.698 kb on - strand, within PGA1_c05130 at 505.724 kb on + strand, within PGA1_c05130 at 505.724 kb on + strand, within PGA1_c05130 at 505.732 kb on - strand, within PGA1_c05130 at 505.732 kb on - strand, within PGA1_c05130 at 505.792 kb on + strand, within PGA1_c05130 at 505.813 kb on + strand, within PGA1_c05130 at 505.830 kb on + strand, within PGA1_c05130 at 505.838 kb on - strand, within PGA1_c05130 at 505.881 kb on + strand, within PGA1_c05130 at 505.938 kb on + strand, within PGA1_c05130 at 505.938 kb on + strand, within PGA1_c05130 at 505.943 kb on + strand, within PGA1_c05130 at 505.943 kb on + strand, within PGA1_c05130 at 505.946 kb on - strand, within PGA1_c05130 at 505.946 kb on - strand, within PGA1_c05130 at 506.074 kb on + strand, within PGA1_c05130 at 506.077 kb on - strand, within PGA1_c05130 at 506.088 kb on - strand, within PGA1_c05130 at 506.106 kb on - strand, within PGA1_c05130 at 506.187 kb on + strand, within PGA1_c05130 at 506.189 kb on - strand, within PGA1_c05130 at 506.235 kb on + strand, within PGA1_c05130 at 506.243 kb on - strand, within PGA1_c05130 at 506.243 kb on - strand, within PGA1_c05130 at 506.243 kb on - strand, within PGA1_c05130 at 506.286 kb on + strand, within PGA1_c05130 at 506.286 kb on + strand, within PGA1_c05130 at 506.291 kb on + strand, within PGA1_c05130 at 506.294 kb on - strand, within PGA1_c05130 at 506.294 kb on - strand, within PGA1_c05130 at 506.299 kb on - strand, within PGA1_c05130 at 506.299 kb on - strand, within PGA1_c05130 at 506.299 kb on - strand, within PGA1_c05130 at 506.299 kb on - strand, within PGA1_c05130 at 506.299 kb on - strand, within PGA1_c05130 at 506.300 kb on - strand, within PGA1_c05130 at 506.374 kb on - strand, within PGA1_c05130 at 506.391 kb on + strand, within PGA1_c05130 at 506.416 kb on + strand, within PGA1_c05130 at 506.497 kb on - strand, within PGA1_c05130 at 506.508 kb on + strand, within PGA1_c05130 at 506.516 kb on - strand, within PGA1_c05130 at 506.518 kb on + strand, within PGA1_c05130 at 506.526 kb on - strand, within PGA1_c05130 at 506.526 kb on - strand, within PGA1_c05130 at 506.535 kb on - strand, within PGA1_c05130 at 506.684 kb on + strand, within PGA1_c05130 at 506.797 kb on + strand at 506.829 kb on - strand at 506.871 kb on + strand at 506.872 kb on + strand at 506.962 kb on - strand at 506.962 kb on - strand at 507.093 kb on - strand, within PGA1_c05140 at 507.193 kb on + strand, within PGA1_c05140 at 507.193 kb on + strand, within PGA1_c05140 at 507.193 kb on + strand, within PGA1_c05140 at 507.201 kb on - strand, within PGA1_c05140 at 507.263 kb on - strand, within PGA1_c05140 at 507.281 kb on - strand, within PGA1_c05140 at 507.457 kb on + strand, within PGA1_c05140 at 507.457 kb on + strand, within PGA1_c05140 at 507.457 kb on + strand, within PGA1_c05140 at 507.465 kb on - strand, within PGA1_c05140 at 507.518 kb on + strand, within PGA1_c05140 at 507.525 kb on + strand, within PGA1_c05140 at 507.528 kb on - strand, within PGA1_c05140 at 507.528 kb on - strand, within PGA1_c05140 at 507.533 kb on - strand, within PGA1_c05140 at 507.602 kb on + strand, within PGA1_c05140 at 507.620 kb on - strand, within PGA1_c05140 at 507.647 kb on + strand, within PGA1_c05140 at 507.656 kb on + strand, within PGA1_c05140 at 507.727 kb on + strand, within PGA1_c05140 at 507.734 kb on + strand, within PGA1_c05140 at 507.806 kb on - strand, within PGA1_c05140 at 507.806 kb on - strand, within PGA1_c05140 at 507.806 kb on - strand, within PGA1_c05140 at 507.815 kb on + strand, within PGA1_c05140 at 507.815 kb on + strand, within PGA1_c05140 at 507.869 kb on - strand, within PGA1_c05140 at 507.871 kb on + strand, within PGA1_c05140
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Doxycycline hyclate 0.001 mg/ml remove 504,471 - +0.6 504,474 + -0.3 504,474 + +2.0 504,482 + -0.4 504,490 - +1.5 504,490 - -0.8 504,554 + -1.0 504,562 - +0.6 504,644 + PGA1_c05120 0.14 +0.5 504,652 - PGA1_c05120 0.15 +0.2 504,652 - PGA1_c05120 0.15 +0.7 504,717 - PGA1_c05120 0.21 +0.7 504,787 - PGA1_c05120 0.29 +1.2 504,805 + PGA1_c05120 0.31 +0.5 504,813 - PGA1_c05120 0.32 +0.6 504,815 + PGA1_c05120 0.32 -2.0 504,857 + PGA1_c05120 0.36 +1.4 504,983 - PGA1_c05120 0.50 +1.5 505,004 + PGA1_c05120 0.52 -0.8 505,010 - PGA1_c05120 0.52 -0.8 505,012 - PGA1_c05120 0.53 +0.1 505,022 + PGA1_c05120 0.54 -0.0 505,029 - PGA1_c05120 0.54 -0.7 505,032 + PGA1_c05120 0.55 +0.3 505,073 + PGA1_c05120 0.59 -0.4 505,075 - PGA1_c05120 0.59 +0.5 505,078 + PGA1_c05120 0.60 -0.0 505,085 - PGA1_c05120 0.60 -1.4 505,085 - PGA1_c05120 0.60 -0.6 505,101 - PGA1_c05120 0.62 -0.7 505,118 + PGA1_c05120 0.64 -0.0 505,142 + PGA1_c05120 0.66 +0.7 505,147 + PGA1_c05120 0.67 -1.3 505,147 + PGA1_c05120 0.67 -0.6 505,147 + PGA1_c05120 0.67 -0.1 505,147 + PGA1_c05120 0.67 +0.3 505,150 - PGA1_c05120 0.67 +0.5 505,155 - PGA1_c05120 0.68 +1.3 505,155 - PGA1_c05120 0.68 -0.8 505,155 - PGA1_c05120 0.68 -2.2 505,155 - PGA1_c05120 0.68 +0.3 505,155 - PGA1_c05120 0.68 -0.3 505,155 - PGA1_c05120 0.68 -0.6 505,155 - PGA1_c05120 0.68 -2.1 505,198 + PGA1_c05120 0.72 +1.0 505,198 + PGA1_c05120 0.72 +0.3 505,206 - PGA1_c05120 0.73 +0.0 505,206 - PGA1_c05120 0.73 +0.8 505,206 - PGA1_c05120 0.73 +0.5 505,206 - PGA1_c05120 0.73 -1.8 505,206 - PGA1_c05120 0.73 -2.6 505,286 + PGA1_c05120 0.82 -1.0 505,286 + PGA1_c05120 0.82 +0.7 505,286 + PGA1_c05120 0.82 -0.1 505,313 + PGA1_c05120 0.85 -0.6 505,331 + PGA1_c05120 0.86 -0.9 505,420 - +0.9 505,442 + +0.5 505,447 + +0.8 505,450 - +1.0 505,497 - +0.1 505,497 - -0.9 505,498 - +0.4 505,550 - -1.1 505,611 + PGA1_c05130 0.11 +0.5 505,613 + PGA1_c05130 0.11 +2.2 505,619 - PGA1_c05130 0.11 -0.4 505,619 - PGA1_c05130 0.11 +0.6 505,645 + PGA1_c05130 0.13 -0.5 505,655 + PGA1_c05130 0.14 +0.0 505,698 - PGA1_c05130 0.17 -0.2 505,698 - PGA1_c05130 0.17 -0.6 505,724 + PGA1_c05130 0.19 -0.4 505,724 + PGA1_c05130 0.19 -0.1 505,732 - PGA1_c05130 0.19 -1.4 505,732 - PGA1_c05130 0.19 -1.1 505,792 + PGA1_c05130 0.23 -0.9 505,813 + PGA1_c05130 0.25 -0.4 505,830 + PGA1_c05130 0.26 -0.6 505,838 - PGA1_c05130 0.27 -2.0 505,881 + PGA1_c05130 0.30 -0.6 505,938 + PGA1_c05130 0.34 -1.5 505,938 + PGA1_c05130 0.34 +2.4 505,943 + PGA1_c05130 0.34 +0.1 505,943 + PGA1_c05130 0.34 -0.2 505,946 - PGA1_c05130 0.34 +0.3 505,946 - PGA1_c05130 0.34 -0.8 506,074 + PGA1_c05130 0.43 -1.0 506,077 - PGA1_c05130 0.43 -0.0 506,088 - PGA1_c05130 0.44 -0.7 506,106 - PGA1_c05130 0.45 +0.4 506,187 + PGA1_c05130 0.51 +0.1 506,189 - PGA1_c05130 0.51 -0.2 506,235 + PGA1_c05130 0.54 +0.4 506,243 - PGA1_c05130 0.55 +0.2 506,243 - PGA1_c05130 0.55 +0.2 506,243 - PGA1_c05130 0.55 -1.6 506,286 + PGA1_c05130 0.58 -1.9 506,286 + PGA1_c05130 0.58 -1.4 506,291 + PGA1_c05130 0.58 -0.6 506,294 - PGA1_c05130 0.58 -2.6 506,294 - PGA1_c05130 0.58 -0.4 506,299 - PGA1_c05130 0.59 +0.3 506,299 - PGA1_c05130 0.59 -1.0 506,299 - PGA1_c05130 0.59 +0.8 506,299 - PGA1_c05130 0.59 -0.4 506,299 - PGA1_c05130 0.59 -0.5 506,300 - PGA1_c05130 0.59 +0.1 506,374 - PGA1_c05130 0.64 -0.0 506,391 + PGA1_c05130 0.65 -0.7 506,416 + PGA1_c05130 0.67 -1.0 506,497 - PGA1_c05130 0.73 +0.2 506,508 + PGA1_c05130 0.73 +2.4 506,516 - PGA1_c05130 0.74 -0.8 506,518 + PGA1_c05130 0.74 -0.0 506,526 - PGA1_c05130 0.75 -0.7 506,526 - PGA1_c05130 0.75 +0.1 506,535 - PGA1_c05130 0.75 +0.9 506,684 + PGA1_c05130 0.86 +0.3 506,797 + -0.4 506,829 - -0.4 506,871 + -0.4 506,872 + -0.4 506,962 - +0.4 506,962 - -0.6 507,093 - PGA1_c05140 0.12 -0.4 507,193 + PGA1_c05140 0.20 +0.5 507,193 + PGA1_c05140 0.20 -0.5 507,193 + PGA1_c05140 0.20 +0.4 507,201 - PGA1_c05140 0.21 +1.4 507,263 - PGA1_c05140 0.26 -2.7 507,281 - PGA1_c05140 0.27 -1.2 507,457 + PGA1_c05140 0.41 -0.0 507,457 + PGA1_c05140 0.41 -1.5 507,457 + PGA1_c05140 0.41 -1.6 507,465 - PGA1_c05140 0.42 -0.8 507,518 + PGA1_c05140 0.46 -1.0 507,525 + PGA1_c05140 0.47 +0.2 507,528 - PGA1_c05140 0.47 +0.5 507,528 - PGA1_c05140 0.47 -0.6 507,533 - PGA1_c05140 0.47 -0.5 507,602 + PGA1_c05140 0.53 -0.2 507,620 - PGA1_c05140 0.54 -0.8 507,647 + PGA1_c05140 0.56 -1.4 507,656 + PGA1_c05140 0.57 +0.5 507,727 + PGA1_c05140 0.63 +0.6 507,734 + PGA1_c05140 0.63 -0.4 507,806 - PGA1_c05140 0.69 +0.6 507,806 - PGA1_c05140 0.69 +0.2 507,806 - PGA1_c05140 0.69 -0.6 507,815 + PGA1_c05140 0.70 -0.3 507,815 + PGA1_c05140 0.70 -0.5 507,869 - PGA1_c05140 0.74 -1.6 507,871 + PGA1_c05140 0.74 -0.3
Or see this region's nucleotide sequence