Experiment: m.b. Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c00440 and PGA1_c00450 are separated by 73 nucleotides PGA1_c00450 and PGA1_c00460 are separated by 57 nucleotides PGA1_c00460 and PGA1_c00470 are separated by 169 nucleotides PGA1_c00470 and PGA1_c00480 are separated by 36 nucleotides
PGA1_c00440: PGA1_c00440 - homogentisate 1,2-dioxygenase HmgA, at 48,878 to 50,239
_c00440
PGA1_c00450: PGA1_c00450 - transcriptional regulator, marR family, at 50,313 to 50,753
_c00450
PGA1_c00460: PGA1_c00460 - adenylate cyclase domain-containing protein, at 50,811 to 52,025
_c00460
PGA1_c00470: PGA1_c00470 - hypothetical protein, at 52,195 to 52,506
_c00470
PGA1_c00480: PGA1_c00480 - CAAX prenyl protease-related protein, at 52,543 to 54,171
_c00480
Position (kb) 50
51
52
53 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 49.833 kb on - strand, within PGA1_c00440 at 49.844 kb on + strand, within PGA1_c00440 at 49.852 kb on - strand, within PGA1_c00440 at 49.862 kb on + strand, within PGA1_c00440 at 49.878 kb on - strand, within PGA1_c00440 at 49.878 kb on - strand, within PGA1_c00440 at 49.878 kb on - strand, within PGA1_c00440 at 49.906 kb on + strand, within PGA1_c00440 at 49.909 kb on - strand, within PGA1_c00440 at 49.914 kb on - strand, within PGA1_c00440 at 49.914 kb on - strand, within PGA1_c00440 at 49.916 kb on + strand, within PGA1_c00440 at 49.919 kb on - strand, within PGA1_c00440 at 49.921 kb on + strand, within PGA1_c00440 at 49.924 kb on - strand, within PGA1_c00440 at 49.968 kb on - strand, within PGA1_c00440 at 49.968 kb on - strand, within PGA1_c00440 at 49.979 kb on + strand, within PGA1_c00440 at 50.000 kb on - strand, within PGA1_c00440 at 50.035 kb on + strand, within PGA1_c00440 at 50.044 kb on - strand, within PGA1_c00440 at 50.044 kb on - strand, within PGA1_c00440 at 50.045 kb on + strand, within PGA1_c00440 at 50.045 kb on + strand, within PGA1_c00440 at 50.045 kb on + strand, within PGA1_c00440 at 50.046 kb on + strand, within PGA1_c00440 at 50.053 kb on - strand, within PGA1_c00440 at 50.058 kb on + strand, within PGA1_c00440 at 50.058 kb on + strand, within PGA1_c00440 at 50.060 kb on + strand, within PGA1_c00440 at 50.060 kb on + strand, within PGA1_c00440 at 50.066 kb on - strand, within PGA1_c00440 at 50.066 kb on - strand, within PGA1_c00440 at 50.105 kb on + strand at 50.113 kb on - strand at 50.113 kb on - strand at 50.119 kb on + strand at 50.120 kb on - strand at 50.124 kb on - strand at 50.130 kb on + strand at 50.131 kb on - strand at 50.132 kb on - strand at 50.168 kb on + strand at 50.178 kb on + strand at 50.186 kb on - strand at 50.196 kb on - strand at 50.202 kb on + strand at 50.219 kb on + strand at 50.219 kb on + strand at 50.227 kb on - strand at 50.248 kb on + strand at 50.268 kb on - strand at 50.298 kb on + strand at 50.303 kb on + strand at 50.303 kb on + strand at 50.303 kb on + strand at 50.303 kb on + strand at 50.303 kb on + strand at 50.303 kb on + strand at 50.303 kb on + strand at 50.303 kb on + strand at 50.303 kb on + strand at 50.303 kb on + strand at 50.303 kb on + strand at 50.303 kb on + strand at 50.303 kb on + strand at 50.311 kb on - strand at 50.311 kb on - strand at 50.311 kb on - strand at 50.311 kb on - strand at 50.311 kb on - strand at 50.311 kb on - strand at 50.311 kb on - strand at 50.311 kb on - strand at 50.311 kb on - strand at 50.311 kb on - strand at 50.311 kb on - strand at 50.311 kb on - strand at 50.311 kb on - strand at 50.311 kb on - strand at 50.311 kb on - strand at 50.363 kb on + strand, within PGA1_c00450 at 50.363 kb on + strand, within PGA1_c00450 at 50.371 kb on + strand, within PGA1_c00450 at 50.384 kb on - strand, within PGA1_c00450 at 50.467 kb on - strand, within PGA1_c00450 at 50.482 kb on - strand, within PGA1_c00450 at 50.557 kb on + strand, within PGA1_c00450 at 50.562 kb on + strand, within PGA1_c00450 at 50.603 kb on + strand, within PGA1_c00450 at 50.629 kb on - strand, within PGA1_c00450 at 50.677 kb on - strand, within PGA1_c00450 at 50.704 kb on + strand, within PGA1_c00450 at 50.709 kb on + strand, within PGA1_c00450 at 50.722 kb on - strand at 50.734 kb on - strand at 50.776 kb on + strand at 50.784 kb on - strand at 50.794 kb on + strand at 50.802 kb on - strand at 50.857 kb on + strand at 50.857 kb on + strand at 50.859 kb on + strand at 50.865 kb on - strand at 50.867 kb on - strand at 50.867 kb on - strand at 50.867 kb on - strand at 50.875 kb on + strand at 50.881 kb on + strand at 50.881 kb on + strand at 50.883 kb on - strand at 50.889 kb on - strand at 50.916 kb on - strand at 50.934 kb on + strand, within PGA1_c00460 at 51.016 kb on - strand, within PGA1_c00460 at 51.049 kb on - strand, within PGA1_c00460 at 51.051 kb on - strand, within PGA1_c00460 at 51.084 kb on - strand, within PGA1_c00460 at 51.118 kb on - strand, within PGA1_c00460 at 51.118 kb on - strand, within PGA1_c00460 at 51.135 kb on + strand, within PGA1_c00460 at 51.163 kb on + strand, within PGA1_c00460 at 51.173 kb on + strand, within PGA1_c00460 at 51.173 kb on + strand, within PGA1_c00460 at 51.198 kb on - strand, within PGA1_c00460 at 51.233 kb on + strand, within PGA1_c00460 at 51.239 kb on - strand, within PGA1_c00460 at 51.239 kb on - strand, within PGA1_c00460 at 51.256 kb on + strand, within PGA1_c00460 at 51.268 kb on + strand, within PGA1_c00460 at 51.295 kb on + strand, within PGA1_c00460 at 51.295 kb on - strand, within PGA1_c00460 at 51.312 kb on - strand, within PGA1_c00460 at 51.387 kb on + strand, within PGA1_c00460 at 51.398 kb on + strand, within PGA1_c00460 at 51.398 kb on + strand, within PGA1_c00460 at 51.398 kb on + strand, within PGA1_c00460 at 51.420 kb on + strand, within PGA1_c00460 at 51.487 kb on + strand, within PGA1_c00460 at 51.500 kb on - strand, within PGA1_c00460 at 51.502 kb on + strand, within PGA1_c00460 at 51.510 kb on - strand, within PGA1_c00460 at 51.532 kb on + strand, within PGA1_c00460 at 51.590 kb on + strand, within PGA1_c00460 at 51.640 kb on + strand, within PGA1_c00460 at 51.663 kb on - strand, within PGA1_c00460 at 51.663 kb on - strand, within PGA1_c00460 at 51.673 kb on - strand, within PGA1_c00460 at 51.702 kb on - strand, within PGA1_c00460 at 51.726 kb on + strand, within PGA1_c00460 at 51.733 kb on + strand, within PGA1_c00460 at 51.734 kb on - strand, within PGA1_c00460 at 51.738 kb on + strand, within PGA1_c00460 at 51.738 kb on + strand, within PGA1_c00460 at 51.738 kb on + strand, within PGA1_c00460 at 51.738 kb on + strand, within PGA1_c00460 at 51.738 kb on + strand, within PGA1_c00460 at 51.741 kb on - strand, within PGA1_c00460 at 51.746 kb on - strand, within PGA1_c00460 at 51.754 kb on + strand, within PGA1_c00460 at 51.768 kb on + strand, within PGA1_c00460 at 51.774 kb on - strand, within PGA1_c00460 at 51.774 kb on - strand, within PGA1_c00460 at 51.891 kb on - strand, within PGA1_c00460 at 51.906 kb on - strand at 51.906 kb on - strand at 51.930 kb on + strand at 51.943 kb on + strand at 51.943 kb on + strand at 51.943 kb on + strand at 51.943 kb on + strand at 51.951 kb on - strand at 51.951 kb on - strand at 51.951 kb on - strand at 51.961 kb on - strand at 51.961 kb on - strand at 51.982 kb on + strand at 51.990 kb on - strand at 52.004 kb on - strand at 52.019 kb on + strand at 52.109 kb on - strand at 52.111 kb on + strand at 52.117 kb on + strand at 52.117 kb on + strand at 52.117 kb on + strand at 52.117 kb on + strand at 52.118 kb on + strand at 52.118 kb on + strand at 52.118 kb on + strand at 52.118 kb on + strand at 52.119 kb on - strand at 52.126 kb on - strand at 52.126 kb on - strand at 52.126 kb on - strand at 52.153 kb on + strand at 52.154 kb on + strand at 52.158 kb on + strand at 52.158 kb on + strand at 52.158 kb on + strand at 52.161 kb on - strand at 52.167 kb on - strand at 52.169 kb on + strand at 52.172 kb on - strand at 52.172 kb on - strand at 52.183 kb on + strand at 52.183 kb on + strand at 52.199 kb on + strand at 52.207 kb on - strand at 52.209 kb on + strand at 52.209 kb on + strand at 52.217 kb on - strand at 52.217 kb on - strand at 52.273 kb on - strand, within PGA1_c00470 at 52.321 kb on + strand, within PGA1_c00470 at 52.323 kb on - strand, within PGA1_c00470 at 52.329 kb on - strand, within PGA1_c00470 at 52.396 kb on - strand, within PGA1_c00470 at 52.421 kb on + strand, within PGA1_c00470 at 52.421 kb on + strand, within PGA1_c00470 at 52.421 kb on + strand, within PGA1_c00470 at 52.421 kb on + strand, within PGA1_c00470 at 52.426 kb on + strand, within PGA1_c00470 at 52.429 kb on - strand, within PGA1_c00470 at 52.429 kb on - strand, within PGA1_c00470 at 52.429 kb on - strand, within PGA1_c00470 at 52.464 kb on - strand, within PGA1_c00470 at 52.477 kb on - strand at 52.490 kb on + strand at 52.500 kb on + strand at 52.541 kb on + strand at 52.549 kb on - strand at 52.554 kb on - strand at 52.554 kb on - strand at 52.632 kb on - strand at 52.634 kb on + strand at 52.634 kb on + strand at 52.642 kb on + strand at 52.642 kb on - strand at 52.829 kb on - strand, within PGA1_c00480 at 52.832 kb on - strand, within PGA1_c00480 at 52.847 kb on - strand, within PGA1_c00480 at 52.850 kb on + strand, within PGA1_c00480 at 52.858 kb on - strand, within PGA1_c00480 at 52.869 kb on + strand, within PGA1_c00480 at 52.877 kb on - strand, within PGA1_c00480 at 52.882 kb on - strand, within PGA1_c00480 at 52.896 kb on + strand, within PGA1_c00480 at 52.953 kb on + strand, within PGA1_c00480 at 52.975 kb on + strand, within PGA1_c00480 at 52.980 kb on + strand, within PGA1_c00480 at 52.980 kb on + strand, within PGA1_c00480 at 52.980 kb on + strand, within PGA1_c00480 at 52.983 kb on - strand, within PGA1_c00480 at 52.983 kb on - strand, within PGA1_c00480 at 52.983 kb on - strand, within PGA1_c00480 at 52.985 kb on + strand, within PGA1_c00480
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Doxycycline hyclate 0.001 mg/ml remove 49,833 - PGA1_c00440 0.70 -1.4 49,844 + PGA1_c00440 0.71 +0.4 49,852 - PGA1_c00440 0.72 -0.2 49,862 + PGA1_c00440 0.72 -0.4 49,878 - PGA1_c00440 0.73 -0.1 49,878 - PGA1_c00440 0.73 -0.3 49,878 - PGA1_c00440 0.73 +0.8 49,906 + PGA1_c00440 0.75 +0.7 49,909 - PGA1_c00440 0.76 +0.4 49,914 - PGA1_c00440 0.76 +0.8 49,914 - PGA1_c00440 0.76 -1.8 49,916 + PGA1_c00440 0.76 -0.1 49,919 - PGA1_c00440 0.76 -4.0 49,921 + PGA1_c00440 0.77 -0.8 49,924 - PGA1_c00440 0.77 +0.1 49,968 - PGA1_c00440 0.80 -1.4 49,968 - PGA1_c00440 0.80 +1.7 49,979 + PGA1_c00440 0.81 -0.2 50,000 - PGA1_c00440 0.82 +0.9 50,035 + PGA1_c00440 0.85 -0.6 50,044 - PGA1_c00440 0.86 +0.5 50,044 - PGA1_c00440 0.86 -0.1 50,045 + PGA1_c00440 0.86 +0.6 50,045 + PGA1_c00440 0.86 -0.8 50,045 + PGA1_c00440 0.86 -1.6 50,046 + PGA1_c00440 0.86 +0.4 50,053 - PGA1_c00440 0.86 +1.0 50,058 + PGA1_c00440 0.87 +0.5 50,058 + PGA1_c00440 0.87 -0.3 50,060 + PGA1_c00440 0.87 -1.2 50,060 + PGA1_c00440 0.87 -0.6 50,066 - PGA1_c00440 0.87 -1.2 50,066 - PGA1_c00440 0.87 +0.0 50,105 + -0.1 50,113 - +0.1 50,113 - -0.1 50,119 + -0.1 50,120 - +1.1 50,124 - -1.5 50,130 + +0.6 50,131 - +2.0 50,132 - -0.5 50,168 + -0.3 50,178 + -0.6 50,186 - +0.3 50,196 - +0.9 50,202 + -0.1 50,219 + +0.8 50,219 + +0.2 50,227 - -0.2 50,248 + +0.3 50,268 - -1.2 50,298 + +0.3 50,303 + +0.9 50,303 + -1.8 50,303 + -1.0 50,303 + +0.4 50,303 + -0.6 50,303 + +0.7 50,303 + +1.0 50,303 + -0.5 50,303 + -2.6 50,303 + -0.2 50,303 + +0.8 50,303 + -0.2 50,303 + +1.9 50,311 - -1.7 50,311 - -0.3 50,311 - -2.4 50,311 - -0.1 50,311 - +2.0 50,311 - -0.4 50,311 - +0.8 50,311 - +1.4 50,311 - -0.3 50,311 - -0.2 50,311 - +0.6 50,311 - -0.0 50,311 - -0.6 50,311 - +0.5 50,311 - +1.8 50,363 + PGA1_c00450 0.11 +0.3 50,363 + PGA1_c00450 0.11 -0.4 50,371 + PGA1_c00450 0.13 +1.3 50,384 - PGA1_c00450 0.16 +0.0 50,467 - PGA1_c00450 0.35 -0.7 50,482 - PGA1_c00450 0.38 -1.3 50,557 + PGA1_c00450 0.55 -0.3 50,562 + PGA1_c00450 0.56 -1.0 50,603 + PGA1_c00450 0.66 -0.1 50,629 - PGA1_c00450 0.72 -0.6 50,677 - PGA1_c00450 0.83 +1.0 50,704 + PGA1_c00450 0.89 -0.3 50,709 + PGA1_c00450 0.90 -0.5 50,722 - -1.7 50,734 - +0.2 50,776 + +0.4 50,784 - -0.8 50,794 + +0.4 50,802 - -1.2 50,857 + +0.6 50,857 + -0.6 50,859 + -1.5 50,865 - -0.6 50,867 - -2.0 50,867 - -0.4 50,867 - -0.1 50,875 + -0.1 50,881 + -1.3 50,881 + -0.0 50,883 - -1.5 50,889 - -0.2 50,916 - -1.5 50,934 + PGA1_c00460 0.10 -2.6 51,016 - PGA1_c00460 0.17 -1.4 51,049 - PGA1_c00460 0.20 +0.4 51,051 - PGA1_c00460 0.20 -2.5 51,084 - PGA1_c00460 0.22 -0.7 51,118 - PGA1_c00460 0.25 -0.8 51,118 - PGA1_c00460 0.25 -0.2 51,135 + PGA1_c00460 0.27 -1.6 51,163 + PGA1_c00460 0.29 -0.5 51,173 + PGA1_c00460 0.30 -0.1 51,173 + PGA1_c00460 0.30 -0.5 51,198 - PGA1_c00460 0.32 -0.0 51,233 + PGA1_c00460 0.35 -0.8 51,239 - PGA1_c00460 0.35 +0.6 51,239 - PGA1_c00460 0.35 +0.0 51,256 + PGA1_c00460 0.37 -2.0 51,268 + PGA1_c00460 0.38 +0.5 51,295 + PGA1_c00460 0.40 -1.7 51,295 - PGA1_c00460 0.40 -1.1 51,312 - PGA1_c00460 0.41 +0.3 51,387 + PGA1_c00460 0.47 -0.5 51,398 + PGA1_c00460 0.48 -2.8 51,398 + PGA1_c00460 0.48 -0.4 51,398 + PGA1_c00460 0.48 -0.7 51,420 + PGA1_c00460 0.50 -0.9 51,487 + PGA1_c00460 0.56 -1.0 51,500 - PGA1_c00460 0.57 +0.5 51,502 + PGA1_c00460 0.57 -1.2 51,510 - PGA1_c00460 0.58 -1.5 51,532 + PGA1_c00460 0.59 +2.0 51,590 + PGA1_c00460 0.64 -0.4 51,640 + PGA1_c00460 0.68 +0.0 51,663 - PGA1_c00460 0.70 -1.7 51,663 - PGA1_c00460 0.70 +0.9 51,673 - PGA1_c00460 0.71 -0.2 51,702 - PGA1_c00460 0.73 +0.2 51,726 + PGA1_c00460 0.75 +0.6 51,733 + PGA1_c00460 0.76 -1.8 51,734 - PGA1_c00460 0.76 -0.4 51,738 + PGA1_c00460 0.76 -0.6 51,738 + PGA1_c00460 0.76 +0.4 51,738 + PGA1_c00460 0.76 -1.6 51,738 + PGA1_c00460 0.76 -0.8 51,738 + PGA1_c00460 0.76 -0.4 51,741 - PGA1_c00460 0.77 -1.2 51,746 - PGA1_c00460 0.77 +0.3 51,754 + PGA1_c00460 0.78 +0.3 51,768 + PGA1_c00460 0.79 -2.3 51,774 - PGA1_c00460 0.79 -0.7 51,774 - PGA1_c00460 0.79 -0.9 51,891 - PGA1_c00460 0.89 -0.3 51,906 - +0.6 51,906 - -0.3 51,930 + +1.2 51,943 + -1.7 51,943 + -0.6 51,943 + -0.8 51,943 + +0.6 51,951 - -1.4 51,951 - +2.9 51,951 - -0.1 51,961 - -0.8 51,961 - -0.6 51,982 + -1.1 51,990 - -0.3 52,004 - -1.2 52,019 + -1.8 52,109 - -2.8 52,111 + +2.3 52,117 + +0.0 52,117 + +0.3 52,117 + -0.1 52,117 + -1.2 52,118 + -2.3 52,118 + -1.7 52,118 + -0.5 52,118 + -0.3 52,119 - -0.1 52,126 - +0.1 52,126 - +1.1 52,126 - -1.2 52,153 + +0.0 52,154 + +0.2 52,158 + +0.0 52,158 + -0.8 52,158 + -0.3 52,161 - -0.4 52,167 - -0.3 52,169 + -1.7 52,172 - -1.5 52,172 - +0.9 52,183 + -2.7 52,183 + -2.5 52,199 + -1.8 52,207 - +0.2 52,209 + +0.4 52,209 + -0.8 52,217 - -2.4 52,217 - -0.8 52,273 - PGA1_c00470 0.25 +1.6 52,321 + PGA1_c00470 0.40 -2.8 52,323 - PGA1_c00470 0.41 +0.2 52,329 - PGA1_c00470 0.43 -0.6 52,396 - PGA1_c00470 0.64 +2.3 52,421 + PGA1_c00470 0.72 -0.3 52,421 + PGA1_c00470 0.72 -0.8 52,421 + PGA1_c00470 0.72 -1.7 52,421 + PGA1_c00470 0.72 -1.6 52,426 + PGA1_c00470 0.74 -0.2 52,429 - PGA1_c00470 0.75 -0.3 52,429 - PGA1_c00470 0.75 +0.1 52,429 - PGA1_c00470 0.75 +2.6 52,464 - PGA1_c00470 0.86 +0.3 52,477 - +0.0 52,490 + +0.7 52,500 + +0.1 52,541 + +0.0 52,549 - +0.4 52,554 - +0.2 52,554 - +0.4 52,632 - -0.9 52,634 + +0.3 52,634 + +0.4 52,642 + +0.3 52,642 - +0.8 52,829 - PGA1_c00480 0.18 +0.4 52,832 - PGA1_c00480 0.18 +0.5 52,847 - PGA1_c00480 0.19 +2.0 52,850 + PGA1_c00480 0.19 +1.4 52,858 - PGA1_c00480 0.19 -2.5 52,869 + PGA1_c00480 0.20 -0.8 52,877 - PGA1_c00480 0.21 -0.9 52,882 - PGA1_c00480 0.21 -1.9 52,896 + PGA1_c00480 0.22 +0.6 52,953 + PGA1_c00480 0.25 +1.5 52,975 + PGA1_c00480 0.27 +0.1 52,980 + PGA1_c00480 0.27 -0.8 52,980 + PGA1_c00480 0.27 +1.0 52,980 + PGA1_c00480 0.27 -0.5 52,983 - PGA1_c00480 0.27 -1.0 52,983 - PGA1_c00480 0.27 +0.4 52,983 - PGA1_c00480 0.27 -0.3 52,985 + PGA1_c00480 0.27 -1.4
Or see this region's nucleotide sequence