Experiment: m.b. Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_262p00150 and PGA1_262p00160 are separated by 95 nucleotides PGA1_262p00160 and PGA1_262p00170 overlap by 4 nucleotides PGA1_262p00170 and PGA1_262p00180 are separated by 88 nucleotides
PGA1_262p00150: PGA1_262p00150 - methyl-accepting chemotaxis protein, at 17,990 to 20,662
_262p00150
PGA1_262p00160: PGA1_262p00160 - Branched-chain amino acid transport protein (AzlD)., at 20,758 to 21,057
_262p00160
PGA1_262p00170: PGA1_262p00170 - Predicted branched-chain amino acid permease (azaleucine resistance), at 21,054 to 21,770
_262p00170
PGA1_262p00180: PGA1_262p00180 - transcriptional regulator, AsnC family, at 21,859 to 22,332
_262p00180
Position (kb)
20
21
22 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 19.769 kb on - strand, within PGA1_262p00150 at 19.772 kb on - strand, within PGA1_262p00150 at 19.774 kb on - strand, within PGA1_262p00150 at 19.796 kb on + strand, within PGA1_262p00150 at 19.802 kb on + strand, within PGA1_262p00150 at 19.817 kb on - strand, within PGA1_262p00150 at 19.870 kb on - strand, within PGA1_262p00150 at 20.035 kb on + strand, within PGA1_262p00150 at 20.140 kb on + strand, within PGA1_262p00150 at 20.329 kb on + strand, within PGA1_262p00150 at 20.329 kb on + strand, within PGA1_262p00150 at 20.337 kb on - strand, within PGA1_262p00150 at 20.416 kb on + strand at 20.416 kb on + strand at 20.416 kb on + strand at 20.416 kb on + strand at 20.418 kb on + strand at 20.424 kb on - strand at 20.424 kb on - strand at 20.424 kb on - strand at 20.426 kb on - strand at 20.426 kb on - strand at 20.426 kb on - strand at 20.511 kb on + strand at 20.511 kb on + strand at 20.519 kb on - strand at 20.519 kb on - strand at 20.519 kb on - strand at 20.771 kb on + strand at 20.846 kb on - strand, within PGA1_262p00160 at 20.950 kb on + strand, within PGA1_262p00160 at 21.010 kb on + strand, within PGA1_262p00160 at 21.010 kb on + strand, within PGA1_262p00160 at 21.010 kb on + strand, within PGA1_262p00160 at 21.010 kb on + strand, within PGA1_262p00160 at 21.025 kb on + strand, within PGA1_262p00160 at 21.025 kb on + strand, within PGA1_262p00160 at 21.033 kb on - strand at 21.033 kb on - strand at 21.068 kb on - strand at 21.126 kb on + strand, within PGA1_262p00170 at 21.218 kb on + strand, within PGA1_262p00170 at 21.220 kb on + strand, within PGA1_262p00170 at 21.228 kb on - strand, within PGA1_262p00170 at 21.278 kb on - strand, within PGA1_262p00170 at 21.314 kb on - strand, within PGA1_262p00170 at 21.356 kb on - strand, within PGA1_262p00170 at 21.356 kb on - strand, within PGA1_262p00170 at 21.378 kb on - strand, within PGA1_262p00170 at 21.413 kb on - strand, within PGA1_262p00170 at 21.420 kb on - strand, within PGA1_262p00170 at 21.531 kb on + strand, within PGA1_262p00170 at 21.546 kb on + strand, within PGA1_262p00170 at 21.687 kb on - strand, within PGA1_262p00170 at 21.786 kb on + strand at 21.914 kb on - strand, within PGA1_262p00180 at 21.914 kb on - strand, within PGA1_262p00180 at 21.939 kb on - strand, within PGA1_262p00180 at 21.968 kb on - strand, within PGA1_262p00180 at 21.981 kb on - strand, within PGA1_262p00180 at 21.981 kb on - strand, within PGA1_262p00180 at 22.001 kb on + strand, within PGA1_262p00180 at 22.036 kb on + strand, within PGA1_262p00180 at 22.052 kb on + strand, within PGA1_262p00180 at 22.052 kb on + strand, within PGA1_262p00180 at 22.052 kb on + strand, within PGA1_262p00180 at 22.052 kb on + strand, within PGA1_262p00180 at 22.052 kb on + strand, within PGA1_262p00180
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Doxycycline hyclate 0.001 mg/ml remove 19,769 - PGA1_262p00150 0.67 -0.6 19,772 - PGA1_262p00150 0.67 -3.2 19,774 - PGA1_262p00150 0.67 +1.3 19,796 + PGA1_262p00150 0.68 +0.4 19,802 + PGA1_262p00150 0.68 +0.7 19,817 - PGA1_262p00150 0.68 +0.3 19,870 - PGA1_262p00150 0.70 -0.1 20,035 + PGA1_262p00150 0.77 -0.1 20,140 + PGA1_262p00150 0.80 -2.7 20,329 + PGA1_262p00150 0.88 +0.7 20,329 + PGA1_262p00150 0.88 -0.3 20,337 - PGA1_262p00150 0.88 -0.2 20,416 + +0.7 20,416 + +1.1 20,416 + +0.7 20,416 + -0.8 20,418 + +0.1 20,424 - +1.9 20,424 - +1.0 20,424 - -1.2 20,426 - +1.4 20,426 - -0.1 20,426 - -0.7 20,511 + -0.3 20,511 + -0.4 20,519 - +2.2 20,519 - +0.7 20,519 - +0.6 20,771 + -0.6 20,846 - PGA1_262p00160 0.29 +0.1 20,950 + PGA1_262p00160 0.64 +0.1 21,010 + PGA1_262p00160 0.84 +1.9 21,010 + PGA1_262p00160 0.84 +1.8 21,010 + PGA1_262p00160 0.84 -0.1 21,010 + PGA1_262p00160 0.84 +0.3 21,025 + PGA1_262p00160 0.89 +2.1 21,025 + PGA1_262p00160 0.89 +0.4 21,033 - -0.2 21,033 - -0.4 21,068 - -0.5 21,126 + PGA1_262p00170 0.10 -0.2 21,218 + PGA1_262p00170 0.23 +1.5 21,220 + PGA1_262p00170 0.23 -1.7 21,228 - PGA1_262p00170 0.24 +1.7 21,278 - PGA1_262p00170 0.31 +0.5 21,314 - PGA1_262p00170 0.36 +0.1 21,356 - PGA1_262p00170 0.42 -0.8 21,356 - PGA1_262p00170 0.42 -0.7 21,378 - PGA1_262p00170 0.45 +0.3 21,413 - PGA1_262p00170 0.50 -0.6 21,420 - PGA1_262p00170 0.51 -0.2 21,531 + PGA1_262p00170 0.67 -0.4 21,546 + PGA1_262p00170 0.69 +0.2 21,687 - PGA1_262p00170 0.88 +0.3 21,786 + -0.5 21,914 - PGA1_262p00180 0.12 -0.7 21,914 - PGA1_262p00180 0.12 +0.5 21,939 - PGA1_262p00180 0.17 +2.4 21,968 - PGA1_262p00180 0.23 -0.7 21,981 - PGA1_262p00180 0.26 -0.9 21,981 - PGA1_262p00180 0.26 +0.1 22,001 + PGA1_262p00180 0.30 +0.1 22,036 + PGA1_262p00180 0.37 +0.8 22,052 + PGA1_262p00180 0.41 -1.4 22,052 + PGA1_262p00180 0.41 +1.1 22,052 + PGA1_262p00180 0.41 +0.1 22,052 + PGA1_262p00180 0.41 +1.1 22,052 + PGA1_262p00180 0.41 +0.9
Or see this region's nucleotide sequence