Experiment: m.b. Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c29070 and PGA1_c29080 are separated by 55 nucleotides PGA1_c29080 and PGA1_c29090 are separated by 39 nucleotides PGA1_c29090 and PGA1_c29100 are separated by 156 nucleotides
PGA1_c29070: PGA1_c29070 - integral membrane protein PecM, at 3,046,638 to 3,047,507
_c29070
PGA1_c29080: PGA1_c29080 - hypothetical protein, at 3,047,563 to 3,048,066
_c29080
PGA1_c29090: PGA1_c29090 - RNA pyrophosphohydrolase RppH, at 3,048,106 to 3,048,588
_c29090
PGA1_c29100: PGA1_c29100 - 2,4-dienoyl-CoA reductase FadH, at 3,048,745 to 3,050,772
_c29100
Position (kb)
3047
3048
3049 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3046.638 kb on + strand at 3046.638 kb on + strand at 3046.638 kb on + strand at 3046.645 kb on + strand at 3046.645 kb on + strand at 3046.645 kb on + strand at 3046.646 kb on - strand at 3046.651 kb on + strand at 3046.653 kb on - strand at 3046.663 kb on - strand at 3046.688 kb on - strand at 3046.698 kb on - strand at 3046.705 kb on + strand at 3046.816 kb on - strand, within PGA1_c29070 at 3046.981 kb on - strand, within PGA1_c29070 at 3047.030 kb on + strand, within PGA1_c29070 at 3047.044 kb on + strand, within PGA1_c29070 at 3047.120 kb on - strand, within PGA1_c29070 at 3047.136 kb on + strand, within PGA1_c29070 at 3047.263 kb on + strand, within PGA1_c29070 at 3047.264 kb on + strand, within PGA1_c29070 at 3047.384 kb on - strand, within PGA1_c29070 at 3047.384 kb on - strand, within PGA1_c29070 at 3047.428 kb on + strand at 3047.434 kb on + strand at 3047.444 kb on + strand at 3047.452 kb on - strand at 3047.452 kb on - strand at 3047.568 kb on + strand at 3047.568 kb on - strand at 3047.640 kb on + strand, within PGA1_c29080 at 3047.684 kb on - strand, within PGA1_c29080 at 3047.697 kb on + strand, within PGA1_c29080 at 3047.730 kb on - strand, within PGA1_c29080 at 3047.743 kb on + strand, within PGA1_c29080 at 3047.789 kb on - strand, within PGA1_c29080 at 3047.808 kb on - strand, within PGA1_c29080 at 3047.818 kb on - strand, within PGA1_c29080 at 3047.872 kb on + strand, within PGA1_c29080 at 3047.882 kb on + strand, within PGA1_c29080 at 3047.882 kb on + strand, within PGA1_c29080 at 3047.890 kb on - strand, within PGA1_c29080 at 3047.890 kb on - strand, within PGA1_c29080 at 3047.890 kb on - strand, within PGA1_c29080 at 3047.890 kb on - strand, within PGA1_c29080 at 3047.890 kb on - strand, within PGA1_c29080 at 3047.890 kb on - strand, within PGA1_c29080 at 3047.901 kb on + strand, within PGA1_c29080 at 3047.901 kb on + strand, within PGA1_c29080 at 3047.901 kb on + strand, within PGA1_c29080 at 3047.909 kb on - strand, within PGA1_c29080 at 3047.926 kb on - strand, within PGA1_c29080 at 3047.928 kb on + strand, within PGA1_c29080 at 3047.986 kb on - strand, within PGA1_c29080 at 3047.986 kb on - strand, within PGA1_c29080 at 3047.986 kb on - strand, within PGA1_c29080 at 3048.021 kb on - strand at 3048.064 kb on + strand at 3048.080 kb on + strand at 3048.090 kb on + strand at 3048.095 kb on + strand at 3048.098 kb on - strand at 3048.098 kb on - strand at 3048.238 kb on + strand, within PGA1_c29090 at 3048.529 kb on + strand, within PGA1_c29090 at 3048.542 kb on - strand at 3048.548 kb on - strand at 3048.595 kb on + strand at 3048.595 kb on + strand at 3048.595 kb on + strand at 3048.636 kb on - strand at 3048.701 kb on + strand at 3048.709 kb on - strand at 3048.720 kb on + strand at 3048.728 kb on + strand at 3048.821 kb on - strand at 3048.833 kb on + strand at 3048.852 kb on - strand at 3048.901 kb on + strand at 3048.959 kb on - strand, within PGA1_c29100 at 3049.003 kb on + strand, within PGA1_c29100
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Doxycycline hyclate 0.001 mg/ml remove 3,046,638 + +0.3 3,046,638 + +0.6 3,046,638 + -0.1 3,046,645 + -1.3 3,046,645 + +0.9 3,046,645 + -0.8 3,046,646 - +1.2 3,046,651 + +1.5 3,046,653 - -0.9 3,046,663 - +0.6 3,046,688 - +2.9 3,046,698 - -0.1 3,046,705 + -3.0 3,046,816 - PGA1_c29070 0.20 +0.6 3,046,981 - PGA1_c29070 0.39 +0.3 3,047,030 + PGA1_c29070 0.45 -1.1 3,047,044 + PGA1_c29070 0.47 +0.9 3,047,120 - PGA1_c29070 0.55 +1.9 3,047,136 + PGA1_c29070 0.57 -0.9 3,047,263 + PGA1_c29070 0.72 +2.0 3,047,264 + PGA1_c29070 0.72 -2.3 3,047,384 - PGA1_c29070 0.86 +1.3 3,047,384 - PGA1_c29070 0.86 +0.9 3,047,428 + -0.1 3,047,434 + +1.2 3,047,444 + +1.1 3,047,452 - +2.4 3,047,452 - -0.1 3,047,568 + -1.5 3,047,568 - -0.3 3,047,640 + PGA1_c29080 0.15 +1.4 3,047,684 - PGA1_c29080 0.24 +3.4 3,047,697 + PGA1_c29080 0.27 -0.2 3,047,730 - PGA1_c29080 0.33 +0.1 3,047,743 + PGA1_c29080 0.36 -0.1 3,047,789 - PGA1_c29080 0.45 +0.9 3,047,808 - PGA1_c29080 0.49 +1.1 3,047,818 - PGA1_c29080 0.51 -1.5 3,047,872 + PGA1_c29080 0.61 -0.5 3,047,882 + PGA1_c29080 0.63 +1.3 3,047,882 + PGA1_c29080 0.63 +1.9 3,047,890 - PGA1_c29080 0.65 -1.3 3,047,890 - PGA1_c29080 0.65 +0.6 3,047,890 - PGA1_c29080 0.65 +0.2 3,047,890 - PGA1_c29080 0.65 -0.1 3,047,890 - PGA1_c29080 0.65 +0.7 3,047,890 - PGA1_c29080 0.65 -0.9 3,047,901 + PGA1_c29080 0.67 -0.1 3,047,901 + PGA1_c29080 0.67 +1.3 3,047,901 + PGA1_c29080 0.67 +1.7 3,047,909 - PGA1_c29080 0.69 +1.6 3,047,926 - PGA1_c29080 0.72 +0.3 3,047,928 + PGA1_c29080 0.72 +0.7 3,047,986 - PGA1_c29080 0.84 +0.9 3,047,986 - PGA1_c29080 0.84 +2.3 3,047,986 - PGA1_c29080 0.84 -0.3 3,048,021 - +2.7 3,048,064 + -0.9 3,048,080 + +1.7 3,048,090 + +1.4 3,048,095 + -0.7 3,048,098 - +0.9 3,048,098 - +0.1 3,048,238 + PGA1_c29090 0.27 +2.1 3,048,529 + PGA1_c29090 0.88 +1.1 3,048,542 - -0.1 3,048,548 - +1.4 3,048,595 + +1.3 3,048,595 + -0.4 3,048,595 + -1.7 3,048,636 - -0.9 3,048,701 + -2.3 3,048,709 - -0.7 3,048,720 + +0.9 3,048,728 + +1.4 3,048,821 - +2.5 3,048,833 + +1.3 3,048,852 - -1.4 3,048,901 + +3.7 3,048,959 - PGA1_c29100 0.11 +3.2 3,049,003 + PGA1_c29100 0.13 +0.9
Or see this region's nucleotide sequence