Experiment: m.b. Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c02380 and PGA1_c02390 are separated by 89 nucleotides PGA1_c02390 and PGA1_c02400 are separated by 79 nucleotides PGA1_c02400 and PGA1_c02420 are separated by 457 nucleotides
PGA1_c02380: PGA1_c02380 - hypothetical protein, at 215,183 to 216,454
_c02380
PGA1_c02390: PGA1_c02390 - transcriptional regulator, MerR family, at 216,544 to 216,948
_c02390
PGA1_c02400: PGA1_c02400 - putative major facilitator superfamily transporter, at 217,028 to 218,269
_c02400
PGA1_c02420: PGA1_c02420 - V8-like Glu-specific endopeptidase, at 218,727 to 219,440
_c02420
Position (kb) 217
218
219 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 216.057 kb on + strand, within PGA1_c02380 at 216.075 kb on - strand, within PGA1_c02380 at 216.075 kb on - strand, within PGA1_c02380 at 216.090 kb on - strand, within PGA1_c02380 at 216.104 kb on + strand, within PGA1_c02380 at 216.152 kb on + strand, within PGA1_c02380 at 216.171 kb on + strand, within PGA1_c02380 at 216.171 kb on + strand, within PGA1_c02380 at 216.211 kb on + strand, within PGA1_c02380 at 216.316 kb on - strand, within PGA1_c02380 at 216.327 kb on - strand at 216.385 kb on + strand at 216.400 kb on + strand at 216.420 kb on + strand at 216.425 kb on - strand at 216.450 kb on + strand at 216.450 kb on + strand at 216.450 kb on + strand at 216.450 kb on + strand at 216.450 kb on + strand at 216.450 kb on + strand at 216.450 kb on + strand at 216.458 kb on - strand at 216.458 kb on - strand at 216.458 kb on - strand at 216.458 kb on - strand at 216.494 kb on + strand at 216.573 kb on - strand at 216.601 kb on + strand, within PGA1_c02390 at 216.610 kb on + strand, within PGA1_c02390 at 216.623 kb on - strand, within PGA1_c02390 at 216.623 kb on - strand, within PGA1_c02390 at 216.646 kb on + strand, within PGA1_c02390 at 216.665 kb on - strand, within PGA1_c02390 at 216.708 kb on - strand, within PGA1_c02390 at 216.779 kb on + strand, within PGA1_c02390 at 216.858 kb on - strand, within PGA1_c02390 at 216.879 kb on + strand, within PGA1_c02390 at 216.887 kb on - strand, within PGA1_c02390 at 216.924 kb on - strand at 216.926 kb on + strand at 216.934 kb on - strand at 216.990 kb on + strand at 216.990 kb on + strand at 216.990 kb on + strand at 216.990 kb on + strand at 216.990 kb on + strand at 216.990 kb on + strand at 216.990 kb on + strand at 216.990 kb on + strand at 216.992 kb on + strand at 216.998 kb on - strand at 216.998 kb on - strand at 216.998 kb on - strand at 217.000 kb on - strand at 217.000 kb on - strand at 217.039 kb on + strand at 217.039 kb on + strand at 217.039 kb on + strand at 217.047 kb on - strand at 217.047 kb on - strand at 217.047 kb on - strand at 217.234 kb on - strand, within PGA1_c02400 at 217.243 kb on + strand, within PGA1_c02400 at 217.306 kb on - strand, within PGA1_c02400 at 217.336 kb on - strand, within PGA1_c02400 at 217.404 kb on - strand, within PGA1_c02400 at 217.450 kb on + strand, within PGA1_c02400 at 217.450 kb on + strand, within PGA1_c02400 at 217.455 kb on + strand, within PGA1_c02400 at 217.455 kb on + strand, within PGA1_c02400 at 217.458 kb on - strand, within PGA1_c02400 at 217.463 kb on - strand, within PGA1_c02400 at 217.463 kb on - strand, within PGA1_c02400 at 217.463 kb on - strand, within PGA1_c02400 at 217.509 kb on + strand, within PGA1_c02400 at 217.656 kb on + strand, within PGA1_c02400 at 217.708 kb on + strand, within PGA1_c02400 at 217.723 kb on + strand, within PGA1_c02400 at 217.775 kb on - strand, within PGA1_c02400 at 217.797 kb on + strand, within PGA1_c02400 at 217.797 kb on + strand, within PGA1_c02400 at 217.805 kb on - strand, within PGA1_c02400 at 217.805 kb on - strand, within PGA1_c02400 at 217.805 kb on - strand, within PGA1_c02400 at 217.822 kb on + strand, within PGA1_c02400 at 217.864 kb on + strand, within PGA1_c02400 at 217.966 kb on + strand, within PGA1_c02400 at 218.013 kb on + strand, within PGA1_c02400 at 218.065 kb on + strand, within PGA1_c02400 at 218.072 kb on + strand, within PGA1_c02400 at 218.092 kb on + strand, within PGA1_c02400 at 218.249 kb on + strand at 218.249 kb on + strand at 218.249 kb on + strand at 218.249 kb on + strand at 218.257 kb on - strand at 218.267 kb on - strand at 218.267 kb on - strand at 218.282 kb on + strand at 218.283 kb on + strand at 218.283 kb on + strand at 218.283 kb on + strand at 218.290 kb on - strand at 218.291 kb on - strand at 218.291 kb on - strand at 218.291 kb on - strand at 218.293 kb on - strand at 218.327 kb on - strand at 218.367 kb on - strand at 218.439 kb on + strand at 218.440 kb on + strand at 218.447 kb on - strand at 218.454 kb on - strand at 218.454 kb on - strand at 218.454 kb on - strand at 218.479 kb on + strand at 218.479 kb on + strand at 218.479 kb on + strand at 218.479 kb on + strand at 218.479 kb on + strand at 218.479 kb on + strand at 218.479 kb on + strand at 218.479 kb on + strand at 218.479 kb on + strand at 218.479 kb on + strand at 218.485 kb on - strand at 218.487 kb on - strand at 218.487 kb on - strand at 218.551 kb on + strand at 218.571 kb on - strand at 218.596 kb on + strand at 218.596 kb on + strand at 218.596 kb on + strand at 218.596 kb on + strand at 218.597 kb on + strand at 218.604 kb on - strand at 218.615 kb on - strand at 218.649 kb on + strand at 218.650 kb on - strand at 218.673 kb on + strand at 218.686 kb on + strand at 218.686 kb on + strand at 218.686 kb on + strand at 218.686 kb on + strand at 218.686 kb on + strand at 218.686 kb on + strand at 218.686 kb on + strand at 218.686 kb on + strand at 218.686 kb on + strand at 218.686 kb on + strand at 218.686 kb on + strand at 218.686 kb on + strand at 218.686 kb on + strand at 218.691 kb on + strand at 218.691 kb on + strand at 218.694 kb on - strand at 218.694 kb on - strand at 218.694 kb on - strand at 218.694 kb on - strand at 218.698 kb on + strand at 218.698 kb on + strand at 218.699 kb on - strand at 218.699 kb on - strand at 218.706 kb on - strand at 218.706 kb on - strand at 218.707 kb on - strand at 218.774 kb on + strand at 218.774 kb on + strand at 218.774 kb on + strand at 218.774 kb on + strand at 218.774 kb on + strand at 218.782 kb on - strand at 218.782 kb on - strand at 218.812 kb on - strand, within PGA1_c02420 at 218.835 kb on + strand, within PGA1_c02420 at 218.872 kb on - strand, within PGA1_c02420 at 218.996 kb on + strand, within PGA1_c02420 at 219.115 kb on + strand, within PGA1_c02420 at 219.115 kb on - strand, within PGA1_c02420 at 219.156 kb on - strand, within PGA1_c02420 at 219.176 kb on + strand, within PGA1_c02420 at 219.177 kb on + strand, within PGA1_c02420 at 219.185 kb on - strand, within PGA1_c02420 at 219.185 kb on - strand, within PGA1_c02420 at 219.185 kb on - strand, within PGA1_c02420 at 219.232 kb on - strand, within PGA1_c02420 at 219.269 kb on - strand, within PGA1_c02420
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Doxycycline hyclate 0.001 mg/ml remove 216,057 + PGA1_c02380 0.69 +0.1 216,075 - PGA1_c02380 0.70 -1.1 216,075 - PGA1_c02380 0.70 -1.9 216,090 - PGA1_c02380 0.71 -0.2 216,104 + PGA1_c02380 0.72 +2.5 216,152 + PGA1_c02380 0.76 -0.6 216,171 + PGA1_c02380 0.78 -0.2 216,171 + PGA1_c02380 0.78 +0.4 216,211 + PGA1_c02380 0.81 +0.9 216,316 - PGA1_c02380 0.89 -0.1 216,327 - -0.1 216,385 + -1.3 216,400 + -2.4 216,420 + -0.3 216,425 - +0.8 216,450 + -0.1 216,450 + -0.6 216,450 + -1.5 216,450 + +0.6 216,450 + +0.8 216,450 + +1.4 216,450 + +1.0 216,458 - -0.5 216,458 - -0.6 216,458 - +0.9 216,458 - +0.1 216,494 + +0.3 216,573 - +1.0 216,601 + PGA1_c02390 0.14 -1.6 216,610 + PGA1_c02390 0.16 -3.3 216,623 - PGA1_c02390 0.20 -0.9 216,623 - PGA1_c02390 0.20 +0.5 216,646 + PGA1_c02390 0.25 +1.2 216,665 - PGA1_c02390 0.30 -1.0 216,708 - PGA1_c02390 0.40 -0.6 216,779 + PGA1_c02390 0.58 -0.2 216,858 - PGA1_c02390 0.78 -0.7 216,879 + PGA1_c02390 0.83 +1.2 216,887 - PGA1_c02390 0.85 -2.2 216,924 - +0.6 216,926 + +0.3 216,934 - +0.2 216,990 + -1.1 216,990 + -0.1 216,990 + -1.0 216,990 + +1.3 216,990 + +0.2 216,990 + +1.5 216,990 + +0.6 216,990 + -0.6 216,992 + +1.5 216,998 - +0.6 216,998 - -0.6 216,998 - -0.2 217,000 - +0.3 217,000 - -2.3 217,039 + -1.6 217,039 + -0.2 217,039 + +1.4 217,047 - -2.0 217,047 - +0.4 217,047 - +0.2 217,234 - PGA1_c02400 0.17 -0.1 217,243 + PGA1_c02400 0.17 +0.6 217,306 - PGA1_c02400 0.22 -0.3 217,336 - PGA1_c02400 0.25 +0.4 217,404 - PGA1_c02400 0.30 +1.3 217,450 + PGA1_c02400 0.34 +0.8 217,450 + PGA1_c02400 0.34 +1.6 217,455 + PGA1_c02400 0.34 -1.0 217,455 + PGA1_c02400 0.34 +0.2 217,458 - PGA1_c02400 0.35 -0.1 217,463 - PGA1_c02400 0.35 +0.4 217,463 - PGA1_c02400 0.35 -0.5 217,463 - PGA1_c02400 0.35 +0.8 217,509 + PGA1_c02400 0.39 -1.8 217,656 + PGA1_c02400 0.51 +1.1 217,708 + PGA1_c02400 0.55 -0.2 217,723 + PGA1_c02400 0.56 +0.7 217,775 - PGA1_c02400 0.60 -2.8 217,797 + PGA1_c02400 0.62 -0.9 217,797 + PGA1_c02400 0.62 -0.3 217,805 - PGA1_c02400 0.63 +1.0 217,805 - PGA1_c02400 0.63 +0.1 217,805 - PGA1_c02400 0.63 -0.1 217,822 + PGA1_c02400 0.64 +0.6 217,864 + PGA1_c02400 0.67 +0.0 217,966 + PGA1_c02400 0.76 -0.3 218,013 + PGA1_c02400 0.79 +1.0 218,065 + PGA1_c02400 0.83 +0.8 218,072 + PGA1_c02400 0.84 +1.6 218,092 + PGA1_c02400 0.86 -1.9 218,249 + -1.2 218,249 + -0.8 218,249 + +1.2 218,249 + -0.1 218,257 - -0.3 218,267 - +0.6 218,267 - +0.2 218,282 + +0.0 218,283 + -1.3 218,283 + -0.2 218,283 + +0.6 218,290 - -1.0 218,291 - -2.7 218,291 - -1.8 218,291 - +1.1 218,293 - -0.4 218,327 - +1.6 218,367 - +0.7 218,439 + -1.0 218,440 + +0.6 218,447 - -0.1 218,454 - +0.4 218,454 - -0.4 218,454 - -1.3 218,479 + +0.2 218,479 + +1.0 218,479 + -1.6 218,479 + +0.8 218,479 + -2.2 218,479 + +1.9 218,479 + +0.5 218,479 + -0.6 218,479 + -0.1 218,479 + -0.1 218,485 - +0.2 218,487 - +1.0 218,487 - -1.4 218,551 + -0.4 218,571 - -2.9 218,596 + +0.4 218,596 + +1.1 218,596 + +0.4 218,596 + +0.2 218,597 + +0.9 218,604 - -0.9 218,615 - +0.3 218,649 + -0.9 218,650 - -1.0 218,673 + -0.1 218,686 + -0.6 218,686 + +0.8 218,686 + +1.3 218,686 + -0.6 218,686 + +0.6 218,686 + -0.2 218,686 + +1.4 218,686 + -1.6 218,686 + -1.3 218,686 + +1.0 218,686 + +0.1 218,686 + -0.4 218,686 + -0.6 218,691 + -0.2 218,691 + +0.3 218,694 - -0.8 218,694 - +1.0 218,694 - -1.2 218,694 - +0.4 218,698 + +0.7 218,698 + +0.4 218,699 - -0.3 218,699 - -1.3 218,706 - +0.4 218,706 - -0.2 218,707 - +1.9 218,774 + +0.3 218,774 + -0.6 218,774 + +0.6 218,774 + -0.6 218,774 + +0.7 218,782 - -2.0 218,782 - +1.2 218,812 - PGA1_c02420 0.12 +0.0 218,835 + PGA1_c02420 0.15 +0.6 218,872 - PGA1_c02420 0.20 -0.5 218,996 + PGA1_c02420 0.38 +0.2 219,115 + PGA1_c02420 0.54 -1.4 219,115 - PGA1_c02420 0.54 -1.0 219,156 - PGA1_c02420 0.60 +0.7 219,176 + PGA1_c02420 0.63 -1.0 219,177 + PGA1_c02420 0.63 -0.6 219,185 - PGA1_c02420 0.64 -0.9 219,185 - PGA1_c02420 0.64 +0.0 219,185 - PGA1_c02420 0.64 -0.1 219,232 - PGA1_c02420 0.71 -1.0 219,269 - PGA1_c02420 0.76 -0.4
Or see this region's nucleotide sequence