Experiment: m.b. Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c21150 and PGA1_c21160 are separated by 81 nucleotides PGA1_c21160 and PGA1_c21170 are separated by 72 nucleotides PGA1_c21170 and PGA1_c21180 are separated by 12 nucleotides
PGA1_c21150: PGA1_c21150 - Asp/Glu/hydantoin racemase-like protein, at 2,190,090 to 2,190,884
_c21150
PGA1_c21160: PGA1_c21160 - putative metallopeptidase, at 2,190,966 to 2,192,123
_c21160
PGA1_c21170: PGA1_c21170 - dihydrofolate reductase FolA, at 2,192,196 to 2,192,672
_c21170
PGA1_c21180: PGA1_c21180 - thymidylate synthase ThyA, at 2,192,685 to 2,193,518
_c21180
Position (kb)
2190
2191
2192
2193 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2190.075 kb on - strand at 2190.092 kb on + strand at 2190.111 kb on + strand at 2190.111 kb on + strand at 2190.119 kb on - strand at 2190.119 kb on - strand at 2190.146 kb on + strand at 2190.177 kb on + strand, within PGA1_c21150 at 2190.192 kb on + strand, within PGA1_c21150 at 2190.192 kb on + strand, within PGA1_c21150 at 2190.200 kb on - strand, within PGA1_c21150 at 2190.200 kb on - strand, within PGA1_c21150 at 2190.205 kb on - strand, within PGA1_c21150 at 2190.257 kb on + strand, within PGA1_c21150 at 2190.265 kb on - strand, within PGA1_c21150 at 2190.265 kb on - strand, within PGA1_c21150 at 2190.344 kb on + strand, within PGA1_c21150 at 2190.349 kb on + strand, within PGA1_c21150 at 2190.352 kb on - strand, within PGA1_c21150 at 2190.357 kb on - strand, within PGA1_c21150 at 2190.357 kb on - strand, within PGA1_c21150 at 2190.376 kb on + strand, within PGA1_c21150 at 2190.384 kb on - strand, within PGA1_c21150 at 2190.439 kb on + strand, within PGA1_c21150 at 2190.533 kb on + strand, within PGA1_c21150 at 2190.571 kb on + strand, within PGA1_c21150 at 2190.629 kb on + strand, within PGA1_c21150 at 2190.629 kb on + strand, within PGA1_c21150 at 2190.629 kb on + strand, within PGA1_c21150 at 2190.629 kb on + strand, within PGA1_c21150 at 2190.629 kb on + strand, within PGA1_c21150 at 2190.629 kb on + strand, within PGA1_c21150 at 2190.629 kb on + strand, within PGA1_c21150 at 2190.629 kb on + strand, within PGA1_c21150 at 2190.629 kb on + strand, within PGA1_c21150 at 2190.637 kb on - strand, within PGA1_c21150 at 2190.637 kb on - strand, within PGA1_c21150 at 2190.674 kb on + strand, within PGA1_c21150 at 2190.718 kb on + strand, within PGA1_c21150 at 2190.718 kb on + strand, within PGA1_c21150 at 2190.718 kb on + strand, within PGA1_c21150 at 2190.726 kb on - strand, within PGA1_c21150 at 2190.726 kb on - strand, within PGA1_c21150 at 2190.726 kb on - strand, within PGA1_c21150 at 2190.726 kb on - strand, within PGA1_c21150 at 2190.793 kb on - strand, within PGA1_c21150 at 2190.852 kb on + strand at 2190.898 kb on + strand at 2190.903 kb on + strand at 2190.937 kb on - strand at 2190.975 kb on + strand at 2190.976 kb on + strand at 2190.976 kb on + strand at 2190.976 kb on + strand at 2190.976 kb on + strand at 2190.978 kb on - strand at 2190.984 kb on - strand at 2190.984 kb on - strand at 2190.984 kb on - strand at 2190.984 kb on - strand at 2190.984 kb on - strand at 2190.984 kb on - strand at 2190.984 kb on - strand at 2190.984 kb on - strand at 2190.984 kb on - strand at 2190.984 kb on - strand at 2190.984 kb on - strand at 2190.984 kb on - strand at 2190.984 kb on - strand at 2190.991 kb on + strand at 2190.994 kb on - strand at 2191.029 kb on + strand at 2191.081 kb on + strand at 2191.081 kb on + strand at 2191.116 kb on - strand, within PGA1_c21160 at 2191.118 kb on + strand, within PGA1_c21160 at 2191.137 kb on - strand, within PGA1_c21160 at 2191.203 kb on + strand, within PGA1_c21160 at 2191.248 kb on + strand, within PGA1_c21160 at 2191.346 kb on - strand, within PGA1_c21160 at 2191.495 kb on + strand, within PGA1_c21160 at 2191.495 kb on + strand, within PGA1_c21160 at 2191.503 kb on - strand, within PGA1_c21160 at 2191.617 kb on + strand, within PGA1_c21160 at 2191.625 kb on - strand, within PGA1_c21160 at 2191.643 kb on - strand, within PGA1_c21160 at 2191.654 kb on + strand, within PGA1_c21160 at 2191.661 kb on + strand, within PGA1_c21160 at 2191.699 kb on + strand, within PGA1_c21160 at 2191.715 kb on + strand, within PGA1_c21160 at 2191.812 kb on - strand, within PGA1_c21160 at 2191.921 kb on + strand, within PGA1_c21160 at 2191.927 kb on + strand, within PGA1_c21160 at 2191.935 kb on - strand, within PGA1_c21160 at 2191.954 kb on - strand, within PGA1_c21160 at 2191.954 kb on - strand, within PGA1_c21160 at 2191.995 kb on - strand, within PGA1_c21160 at 2192.023 kb on - strand at 2192.133 kb on + strand at 2192.672 kb on + strand at 2192.680 kb on - strand at 2192.680 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Doxycycline hyclate 0.001 mg/ml remove 2,190,075 - -1.1 2,190,092 + -1.3 2,190,111 + +1.9 2,190,111 + +0.0 2,190,119 - +0.4 2,190,119 - -1.1 2,190,146 + +0.4 2,190,177 + PGA1_c21150 0.11 -0.9 2,190,192 + PGA1_c21150 0.13 +2.7 2,190,192 + PGA1_c21150 0.13 -1.1 2,190,200 - PGA1_c21150 0.14 +0.1 2,190,200 - PGA1_c21150 0.14 +0.4 2,190,205 - PGA1_c21150 0.14 -1.8 2,190,257 + PGA1_c21150 0.21 +1.3 2,190,265 - PGA1_c21150 0.22 -0.9 2,190,265 - PGA1_c21150 0.22 -0.3 2,190,344 + PGA1_c21150 0.32 +0.1 2,190,349 + PGA1_c21150 0.33 +0.9 2,190,352 - PGA1_c21150 0.33 +0.2 2,190,357 - PGA1_c21150 0.34 -0.3 2,190,357 - PGA1_c21150 0.34 -0.5 2,190,376 + PGA1_c21150 0.36 +1.0 2,190,384 - PGA1_c21150 0.37 -0.7 2,190,439 + PGA1_c21150 0.44 +0.4 2,190,533 + PGA1_c21150 0.56 +1.3 2,190,571 + PGA1_c21150 0.61 +0.9 2,190,629 + PGA1_c21150 0.68 -0.0 2,190,629 + PGA1_c21150 0.68 +4.0 2,190,629 + PGA1_c21150 0.68 -0.3 2,190,629 + PGA1_c21150 0.68 +0.4 2,190,629 + PGA1_c21150 0.68 +0.7 2,190,629 + PGA1_c21150 0.68 +0.5 2,190,629 + PGA1_c21150 0.68 +0.2 2,190,629 + PGA1_c21150 0.68 +0.7 2,190,629 + PGA1_c21150 0.68 +2.1 2,190,637 - PGA1_c21150 0.69 +1.1 2,190,637 - PGA1_c21150 0.69 +0.3 2,190,674 + PGA1_c21150 0.73 +0.7 2,190,718 + PGA1_c21150 0.79 +0.0 2,190,718 + PGA1_c21150 0.79 +2.3 2,190,718 + PGA1_c21150 0.79 +0.1 2,190,726 - PGA1_c21150 0.80 +0.3 2,190,726 - PGA1_c21150 0.80 +2.3 2,190,726 - PGA1_c21150 0.80 +0.6 2,190,726 - PGA1_c21150 0.80 +0.1 2,190,793 - PGA1_c21150 0.88 +0.7 2,190,852 + -0.3 2,190,898 + -0.9 2,190,903 + +1.3 2,190,937 - +0.1 2,190,975 + +0.3 2,190,976 + +0.6 2,190,976 + +1.9 2,190,976 + +0.7 2,190,976 + -1.3 2,190,978 - +0.4 2,190,984 - -0.1 2,190,984 - +0.3 2,190,984 - +2.5 2,190,984 - +0.5 2,190,984 - -0.9 2,190,984 - -2.1 2,190,984 - -0.6 2,190,984 - -0.9 2,190,984 - +1.0 2,190,984 - -1.3 2,190,984 - +2.2 2,190,984 - -0.1 2,190,984 - +0.9 2,190,991 + -0.7 2,190,994 - +0.7 2,191,029 + +0.3 2,191,081 + +0.7 2,191,081 + -1.3 2,191,116 - PGA1_c21160 0.13 +1.2 2,191,118 + PGA1_c21160 0.13 +1.0 2,191,137 - PGA1_c21160 0.15 -1.3 2,191,203 + PGA1_c21160 0.20 +1.3 2,191,248 + PGA1_c21160 0.24 +1.4 2,191,346 - PGA1_c21160 0.33 +1.1 2,191,495 + PGA1_c21160 0.46 +0.1 2,191,495 + PGA1_c21160 0.46 +1.3 2,191,503 - PGA1_c21160 0.46 +0.7 2,191,617 + PGA1_c21160 0.56 -1.9 2,191,625 - PGA1_c21160 0.57 +0.1 2,191,643 - PGA1_c21160 0.58 -2.8 2,191,654 + PGA1_c21160 0.59 +0.7 2,191,661 + PGA1_c21160 0.60 -1.3 2,191,699 + PGA1_c21160 0.63 +1.0 2,191,715 + PGA1_c21160 0.65 +1.5 2,191,812 - PGA1_c21160 0.73 -0.1 2,191,921 + PGA1_c21160 0.82 -0.5 2,191,927 + PGA1_c21160 0.83 +1.3 2,191,935 - PGA1_c21160 0.84 +2.3 2,191,954 - PGA1_c21160 0.85 -0.6 2,191,954 - PGA1_c21160 0.85 +1.3 2,191,995 - PGA1_c21160 0.89 -0.1 2,192,023 - -1.7 2,192,133 + +0.3 2,192,672 + +0.7 2,192,680 - +1.7 2,192,680 - +0.7
Or see this region's nucleotide sequence