Experiment: m.b. Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c18960 and PGA1_c18970 are separated by 89 nucleotides PGA1_c18970 and PGA1_c18980 overlap by 4 nucleotides
PGA1_c18960: PGA1_c18960 - seryl-tRNA synthetase SerS, at 1,966,428 to 1,967,720
_c18960
PGA1_c18970: PGA1_c18970 - hypothetical protein, at 1,967,810 to 1,968,532
_c18970
PGA1_c18980: PGA1_c18980 - putative fatty acid desaturase, at 1,968,529 to 1,969,542
_c18980
Position (kb)
1967
1968
1969 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1967.796 kb on + strand at 1967.820 kb on + strand at 1967.870 kb on - strand at 1967.872 kb on + strand at 1967.880 kb on - strand at 1967.987 kb on - strand, within PGA1_c18970 at 1967.991 kb on + strand, within PGA1_c18970 at 1967.991 kb on + strand, within PGA1_c18970 at 1967.997 kb on + strand, within PGA1_c18970 at 1967.999 kb on - strand, within PGA1_c18970 at 1967.999 kb on - strand, within PGA1_c18970 at 1968.032 kb on - strand, within PGA1_c18970 at 1968.044 kb on - strand, within PGA1_c18970 at 1968.054 kb on - strand, within PGA1_c18970 at 1968.155 kb on - strand, within PGA1_c18970 at 1968.155 kb on - strand, within PGA1_c18970 at 1968.155 kb on - strand, within PGA1_c18970 at 1968.155 kb on - strand, within PGA1_c18970 at 1968.221 kb on + strand, within PGA1_c18970 at 1968.337 kb on + strand, within PGA1_c18970 at 1968.337 kb on + strand, within PGA1_c18970 at 1968.345 kb on - strand, within PGA1_c18970 at 1968.345 kb on - strand, within PGA1_c18970 at 1968.347 kb on + strand, within PGA1_c18970 at 1968.382 kb on + strand, within PGA1_c18970 at 1968.405 kb on + strand, within PGA1_c18970 at 1968.501 kb on - strand at 1968.537 kb on + strand at 1968.582 kb on + strand at 1968.615 kb on + strand at 1968.814 kb on + strand, within PGA1_c18980 at 1968.818 kb on + strand, within PGA1_c18980 at 1968.829 kb on - strand, within PGA1_c18980 at 1968.946 kb on + strand, within PGA1_c18980 at 1968.974 kb on + strand, within PGA1_c18980 at 1968.974 kb on + strand, within PGA1_c18980 at 1968.974 kb on + strand, within PGA1_c18980 at 1968.974 kb on + strand, within PGA1_c18980 at 1968.982 kb on - strand, within PGA1_c18980 at 1968.982 kb on - strand, within PGA1_c18980 at 1968.982 kb on - strand, within PGA1_c18980 at 1969.007 kb on + strand, within PGA1_c18980 at 1969.012 kb on + strand, within PGA1_c18980 at 1969.020 kb on - strand, within PGA1_c18980 at 1969.042 kb on - strand, within PGA1_c18980 at 1969.078 kb on + strand, within PGA1_c18980 at 1969.086 kb on - strand, within PGA1_c18980 at 1969.148 kb on - strand, within PGA1_c18980 at 1969.173 kb on + strand, within PGA1_c18980 at 1969.173 kb on + strand, within PGA1_c18980 at 1969.181 kb on + strand, within PGA1_c18980 at 1969.189 kb on - strand, within PGA1_c18980 at 1969.228 kb on - strand, within PGA1_c18980 at 1969.233 kb on - strand, within PGA1_c18980 at 1969.236 kb on + strand, within PGA1_c18980 at 1969.265 kb on - strand, within PGA1_c18980 at 1969.266 kb on + strand, within PGA1_c18980 at 1969.266 kb on + strand, within PGA1_c18980 at 1969.266 kb on + strand, within PGA1_c18980 at 1969.274 kb on - strand, within PGA1_c18980 at 1969.281 kb on - strand, within PGA1_c18980 at 1969.381 kb on + strand, within PGA1_c18980 at 1969.417 kb on + strand, within PGA1_c18980 at 1969.425 kb on - strand, within PGA1_c18980 at 1969.453 kb on + strand at 1969.457 kb on - strand at 1969.461 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Doxycycline hyclate 0.001 mg/ml remove 1,967,796 + +1.2 1,967,820 + +0.7 1,967,870 - -1.3 1,967,872 + +0.7 1,967,880 - -0.3 1,967,987 - PGA1_c18970 0.24 -0.9 1,967,991 + PGA1_c18970 0.25 +0.5 1,967,991 + PGA1_c18970 0.25 -0.2 1,967,997 + PGA1_c18970 0.26 +0.8 1,967,999 - PGA1_c18970 0.26 +0.5 1,967,999 - PGA1_c18970 0.26 +0.4 1,968,032 - PGA1_c18970 0.31 +1.7 1,968,044 - PGA1_c18970 0.32 +0.7 1,968,054 - PGA1_c18970 0.34 -0.2 1,968,155 - PGA1_c18970 0.48 +0.5 1,968,155 - PGA1_c18970 0.48 -1.9 1,968,155 - PGA1_c18970 0.48 +1.7 1,968,155 - PGA1_c18970 0.48 +0.3 1,968,221 + PGA1_c18970 0.57 +1.4 1,968,337 + PGA1_c18970 0.73 -0.3 1,968,337 + PGA1_c18970 0.73 -0.3 1,968,345 - PGA1_c18970 0.74 -2.2 1,968,345 - PGA1_c18970 0.74 +2.1 1,968,347 + PGA1_c18970 0.74 +0.9 1,968,382 + PGA1_c18970 0.79 +0.9 1,968,405 + PGA1_c18970 0.82 +0.7 1,968,501 - +1.4 1,968,537 + -1.2 1,968,582 + +0.6 1,968,615 + -0.1 1,968,814 + PGA1_c18980 0.28 -0.2 1,968,818 + PGA1_c18980 0.29 +0.1 1,968,829 - PGA1_c18980 0.30 -0.3 1,968,946 + PGA1_c18980 0.41 +0.1 1,968,974 + PGA1_c18980 0.44 -0.2 1,968,974 + PGA1_c18980 0.44 +0.2 1,968,974 + PGA1_c18980 0.44 +3.0 1,968,974 + PGA1_c18980 0.44 +0.5 1,968,982 - PGA1_c18980 0.45 -0.6 1,968,982 - PGA1_c18980 0.45 -2.3 1,968,982 - PGA1_c18980 0.45 +0.5 1,969,007 + PGA1_c18980 0.47 +0.5 1,969,012 + PGA1_c18980 0.48 -0.0 1,969,020 - PGA1_c18980 0.48 +0.3 1,969,042 - PGA1_c18980 0.51 +0.8 1,969,078 + PGA1_c18980 0.54 +2.2 1,969,086 - PGA1_c18980 0.55 -0.2 1,969,148 - PGA1_c18980 0.61 +0.9 1,969,173 + PGA1_c18980 0.64 -2.5 1,969,173 + PGA1_c18980 0.64 -1.3 1,969,181 + PGA1_c18980 0.64 +2.0 1,969,189 - PGA1_c18980 0.65 +0.1 1,969,228 - PGA1_c18980 0.69 -0.2 1,969,233 - PGA1_c18980 0.69 -0.9 1,969,236 + PGA1_c18980 0.70 -0.3 1,969,265 - PGA1_c18980 0.73 -0.3 1,969,266 + PGA1_c18980 0.73 +2.0 1,969,266 + PGA1_c18980 0.73 -0.5 1,969,266 + PGA1_c18980 0.73 +0.1 1,969,274 - PGA1_c18980 0.73 +1.1 1,969,281 - PGA1_c18980 0.74 +0.3 1,969,381 + PGA1_c18980 0.84 -0.3 1,969,417 + PGA1_c18980 0.88 -1.2 1,969,425 - PGA1_c18980 0.88 -1.1 1,969,453 + -0.2 1,969,457 - -0.6 1,969,461 - +0.7
Or see this region's nucleotide sequence