Experiment: m.b. Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c01440 and PGA1_c01460 are separated by 569 nucleotides PGA1_c01460 and PGA1_c01470 overlap by 4 nucleotides PGA1_c01470 and PGA1_c01480 are separated by 6 nucleotides
PGA1_c01440: PGA1_c01440 - DNA-directed RNA polymerase subunit beta', at 137,926 to 142,167
_c01440
PGA1_c01460: PGA1_c01460 - Protein of unknown function (DUF3118)., at 142,737 to 143,561
_c01460
PGA1_c01470: PGA1_c01470 - Permeases of the drug/metabolite transporter (DMT) superfamily, at 143,558 to 144,460
_c01470
PGA1_c01480: PGA1_c01480 - Protein of unknown function (DUF3118)., at 144,467 to 145,243
_c01480
Position (kb)
142
143
144 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 142.079 kb on + strand at 142.148 kb on + strand at 142.203 kb on - strand at 142.250 kb on + strand at 142.251 kb on - strand at 142.273 kb on - strand at 142.344 kb on - strand at 142.367 kb on + strand at 142.375 kb on - strand at 142.375 kb on - strand at 142.376 kb on + strand at 142.376 kb on + strand at 142.384 kb on - strand at 142.384 kb on - strand at 142.392 kb on - strand at 142.438 kb on - strand at 142.438 kb on - strand at 142.516 kb on - strand at 142.584 kb on - strand at 142.623 kb on - strand at 142.630 kb on - strand at 142.649 kb on - strand at 142.720 kb on + strand at 142.720 kb on + strand at 142.720 kb on + strand at 142.723 kb on - strand at 142.728 kb on - strand at 142.761 kb on + strand at 142.774 kb on + strand at 142.774 kb on + strand at 142.782 kb on - strand at 142.787 kb on - strand at 142.806 kb on - strand at 142.900 kb on + strand, within PGA1_c01460 at 142.991 kb on + strand, within PGA1_c01460 at 142.991 kb on + strand, within PGA1_c01460 at 143.008 kb on + strand, within PGA1_c01460 at 143.016 kb on - strand, within PGA1_c01460 at 143.070 kb on - strand, within PGA1_c01460 at 143.123 kb on - strand, within PGA1_c01460 at 143.172 kb on + strand, within PGA1_c01460 at 143.180 kb on - strand, within PGA1_c01460 at 143.180 kb on - strand, within PGA1_c01460 at 143.181 kb on + strand, within PGA1_c01460 at 143.210 kb on - strand, within PGA1_c01460 at 143.227 kb on + strand, within PGA1_c01460 at 143.227 kb on + strand, within PGA1_c01460 at 143.227 kb on + strand, within PGA1_c01460 at 143.235 kb on - strand, within PGA1_c01460 at 143.235 kb on - strand, within PGA1_c01460 at 143.235 kb on - strand, within PGA1_c01460 at 143.235 kb on - strand, within PGA1_c01460 at 143.245 kb on + strand, within PGA1_c01460 at 143.372 kb on - strand, within PGA1_c01460 at 143.374 kb on + strand, within PGA1_c01460 at 143.382 kb on + strand, within PGA1_c01460 at 143.405 kb on + strand, within PGA1_c01460 at 143.410 kb on + strand, within PGA1_c01460 at 143.413 kb on - strand, within PGA1_c01460 at 143.462 kb on + strand, within PGA1_c01460 at 143.470 kb on - strand, within PGA1_c01460 at 143.505 kb on + strand at 143.505 kb on + strand at 143.505 kb on + strand at 143.505 kb on + strand at 143.505 kb on + strand at 143.505 kb on + strand at 143.513 kb on - strand at 143.513 kb on - strand at 143.604 kb on - strand at 143.618 kb on - strand at 143.641 kb on + strand at 143.641 kb on + strand at 143.649 kb on - strand, within PGA1_c01470 at 143.649 kb on - strand, within PGA1_c01470 at 143.679 kb on + strand, within PGA1_c01470 at 143.687 kb on - strand, within PGA1_c01470 at 143.778 kb on + strand, within PGA1_c01470 at 143.786 kb on + strand, within PGA1_c01470 at 143.787 kb on + strand, within PGA1_c01470 at 143.788 kb on + strand, within PGA1_c01470 at 143.831 kb on + strand, within PGA1_c01470 at 143.834 kb on + strand, within PGA1_c01470 at 143.842 kb on - strand, within PGA1_c01470 at 143.842 kb on - strand, within PGA1_c01470 at 143.842 kb on - strand, within PGA1_c01470 at 143.842 kb on - strand, within PGA1_c01470 at 143.956 kb on - strand, within PGA1_c01470 at 143.983 kb on - strand, within PGA1_c01470 at 144.020 kb on + strand, within PGA1_c01470 at 144.022 kb on - strand, within PGA1_c01470 at 144.028 kb on - strand, within PGA1_c01470 at 144.054 kb on + strand, within PGA1_c01470 at 144.120 kb on - strand, within PGA1_c01470 at 144.136 kb on - strand, within PGA1_c01470 at 144.136 kb on - strand, within PGA1_c01470 at 144.147 kb on + strand, within PGA1_c01470 at 144.155 kb on - strand, within PGA1_c01470 at 144.155 kb on - strand, within PGA1_c01470 at 144.165 kb on - strand, within PGA1_c01470 at 144.173 kb on - strand, within PGA1_c01470 at 144.173 kb on - strand, within PGA1_c01470 at 144.209 kb on + strand, within PGA1_c01470 at 144.210 kb on - strand, within PGA1_c01470 at 144.216 kb on + strand, within PGA1_c01470 at 144.435 kb on + strand at 144.435 kb on + strand at 144.548 kb on + strand, within PGA1_c01480
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Doxycycline hyclate 0.001 mg/ml remove 142,079 + +0.0 142,148 + -1.6 142,203 - +0.1 142,250 + +0.0 142,251 - -0.4 142,273 - -0.8 142,344 - -1.1 142,367 + +0.0 142,375 - -1.1 142,375 - +0.8 142,376 + +0.3 142,376 + -0.7 142,384 - +0.2 142,384 - +0.2 142,392 - -0.4 142,438 - +1.0 142,438 - +1.4 142,516 - +0.4 142,584 - +0.0 142,623 - -3.2 142,630 - -0.9 142,649 - -2.5 142,720 + +0.0 142,720 + -1.2 142,720 + -0.6 142,723 - +0.2 142,728 - +0.4 142,761 + +0.0 142,774 + -0.3 142,774 + -0.4 142,782 - +1.1 142,787 - -1.3 142,806 - -1.6 142,900 + PGA1_c01460 0.20 +0.4 142,991 + PGA1_c01460 0.31 -0.1 142,991 + PGA1_c01460 0.31 -2.4 143,008 + PGA1_c01460 0.33 +0.2 143,016 - PGA1_c01460 0.34 -0.8 143,070 - PGA1_c01460 0.40 -0.8 143,123 - PGA1_c01460 0.47 -1.6 143,172 + PGA1_c01460 0.53 +2.0 143,180 - PGA1_c01460 0.54 -1.1 143,180 - PGA1_c01460 0.54 -2.3 143,181 + PGA1_c01460 0.54 -1.7 143,210 - PGA1_c01460 0.57 -0.2 143,227 + PGA1_c01460 0.59 -0.3 143,227 + PGA1_c01460 0.59 -0.6 143,227 + PGA1_c01460 0.59 -2.5 143,235 - PGA1_c01460 0.60 +0.2 143,235 - PGA1_c01460 0.60 -0.9 143,235 - PGA1_c01460 0.60 +0.4 143,235 - PGA1_c01460 0.60 +1.1 143,245 + PGA1_c01460 0.62 -0.5 143,372 - PGA1_c01460 0.77 -0.8 143,374 + PGA1_c01460 0.77 +0.7 143,382 + PGA1_c01460 0.78 +1.8 143,405 + PGA1_c01460 0.81 +0.8 143,410 + PGA1_c01460 0.82 -0.3 143,413 - PGA1_c01460 0.82 -1.0 143,462 + PGA1_c01460 0.88 -0.8 143,470 - PGA1_c01460 0.89 +0.2 143,505 + +1.6 143,505 + +0.9 143,505 + +0.2 143,505 + -0.1 143,505 + +0.0 143,505 + -0.1 143,513 - +2.1 143,513 - -1.2 143,604 - -0.3 143,618 - +0.0 143,641 + +0.7 143,641 + -1.3 143,649 - PGA1_c01470 0.10 +0.9 143,649 - PGA1_c01470 0.10 +0.3 143,679 + PGA1_c01470 0.13 -0.3 143,687 - PGA1_c01470 0.14 -0.9 143,778 + PGA1_c01470 0.24 -1.7 143,786 + PGA1_c01470 0.25 -2.1 143,787 + PGA1_c01470 0.25 -0.1 143,788 + PGA1_c01470 0.25 +0.7 143,831 + PGA1_c01470 0.30 -0.8 143,834 + PGA1_c01470 0.31 -0.2 143,842 - PGA1_c01470 0.31 -0.6 143,842 - PGA1_c01470 0.31 -0.3 143,842 - PGA1_c01470 0.31 -1.5 143,842 - PGA1_c01470 0.31 +0.2 143,956 - PGA1_c01470 0.44 +1.4 143,983 - PGA1_c01470 0.47 -2.1 144,020 + PGA1_c01470 0.51 -0.0 144,022 - PGA1_c01470 0.51 +0.1 144,028 - PGA1_c01470 0.52 +0.1 144,054 + PGA1_c01470 0.55 -0.4 144,120 - PGA1_c01470 0.62 +0.7 144,136 - PGA1_c01470 0.64 -1.2 144,136 - PGA1_c01470 0.64 +0.4 144,147 + PGA1_c01470 0.65 -0.3 144,155 - PGA1_c01470 0.66 -1.4 144,155 - PGA1_c01470 0.66 -1.6 144,165 - PGA1_c01470 0.67 +0.1 144,173 - PGA1_c01470 0.68 -0.2 144,173 - PGA1_c01470 0.68 +0.5 144,209 + PGA1_c01470 0.72 +0.2 144,210 - PGA1_c01470 0.72 -0.1 144,216 + PGA1_c01470 0.73 -1.9 144,435 + +0.4 144,435 + -0.6 144,548 + PGA1_c01480 0.10 +0.5
Or see this region's nucleotide sequence