Experiment: m.b. D-Cycloserine 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c07080 and PGA1_c07090 are separated by 129 nucleotides PGA1_c07090 and PGA1_c07100 are separated by 409 nucleotides PGA1_c07100 and PGA1_c07110 are separated by 53 nucleotides
PGA1_c07080: PGA1_c07080 - small-conductance mechanosensitive channel, at 726,925 to 727,731
_c07080
PGA1_c07090: PGA1_c07090 - large-conductance mechanosensitive channel, at 727,861 to 728,295
_c07090
PGA1_c07100: PGA1_c07100 - RTX toxins and related Ca2+-binding proteins, at 728,705 to 731,062
_c07100
PGA1_c07110: PGA1_c07110 - putative sodium/alanine symporter, at 731,116 to 732,660
_c07110
Position (kb) 728
729
730
731
732 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 727.713 kb on + strand at 727.721 kb on - strand at 727.830 kb on - strand at 727.839 kb on + strand at 727.958 kb on - strand, within PGA1_c07090 at 727.992 kb on - strand, within PGA1_c07090 at 728.045 kb on - strand, within PGA1_c07090 at 728.086 kb on + strand, within PGA1_c07090 at 728.111 kb on + strand, within PGA1_c07090 at 728.111 kb on + strand, within PGA1_c07090 at 728.111 kb on + strand, within PGA1_c07090 at 728.111 kb on + strand, within PGA1_c07090 at 728.111 kb on + strand, within PGA1_c07090 at 728.111 kb on + strand, within PGA1_c07090 at 728.220 kb on - strand, within PGA1_c07090 at 728.307 kb on - strand at 728.342 kb on + strand at 728.347 kb on + strand at 728.349 kb on + strand at 728.349 kb on + strand at 728.349 kb on + strand at 728.357 kb on - strand at 728.466 kb on - strand at 728.510 kb on + strand at 728.512 kb on - strand at 728.513 kb on - strand at 728.515 kb on + strand at 728.562 kb on + strand at 728.562 kb on + strand at 728.569 kb on + strand at 728.570 kb on - strand at 728.570 kb on - strand at 728.570 kb on - strand at 728.577 kb on - strand at 728.602 kb on + strand at 728.624 kb on + strand at 728.634 kb on + strand at 728.639 kb on + strand at 728.664 kb on - strand at 728.674 kb on - strand at 728.685 kb on + strand at 728.698 kb on - strand at 728.698 kb on - strand at 728.748 kb on - strand at 728.753 kb on + strand at 728.756 kb on - strand at 728.763 kb on + strand at 728.843 kb on - strand at 728.847 kb on + strand at 728.847 kb on + strand at 728.855 kb on - strand at 728.855 kb on - strand at 728.930 kb on + strand at 729.014 kb on + strand, within PGA1_c07100 at 729.022 kb on - strand, within PGA1_c07100 at 729.022 kb on - strand, within PGA1_c07100 at 729.029 kb on + strand, within PGA1_c07100 at 729.052 kb on - strand, within PGA1_c07100 at 729.062 kb on - strand, within PGA1_c07100 at 729.078 kb on - strand, within PGA1_c07100 at 729.095 kb on + strand, within PGA1_c07100 at 729.125 kb on - strand, within PGA1_c07100 at 729.197 kb on - strand, within PGA1_c07100 at 729.202 kb on - strand, within PGA1_c07100 at 729.242 kb on - strand, within PGA1_c07100 at 729.253 kb on + strand, within PGA1_c07100 at 729.315 kb on - strand, within PGA1_c07100 at 729.489 kb on - strand, within PGA1_c07100 at 729.495 kb on + strand, within PGA1_c07100 at 729.512 kb on + strand, within PGA1_c07100 at 729.529 kb on - strand, within PGA1_c07100 at 729.532 kb on + strand, within PGA1_c07100 at 729.552 kb on + strand, within PGA1_c07100 at 729.566 kb on - strand, within PGA1_c07100 at 729.566 kb on - strand, within PGA1_c07100 at 729.575 kb on + strand, within PGA1_c07100 at 729.592 kb on + strand, within PGA1_c07100 at 729.602 kb on + strand, within PGA1_c07100 at 729.610 kb on - strand, within PGA1_c07100 at 729.624 kb on - strand, within PGA1_c07100 at 729.640 kb on + strand, within PGA1_c07100 at 729.672 kb on - strand, within PGA1_c07100 at 729.697 kb on + strand, within PGA1_c07100 at 729.697 kb on + strand, within PGA1_c07100 at 729.705 kb on - strand, within PGA1_c07100 at 729.705 kb on - strand, within PGA1_c07100 at 729.751 kb on - strand, within PGA1_c07100 at 729.822 kb on + strand, within PGA1_c07100 at 729.906 kb on - strand, within PGA1_c07100 at 729.932 kb on + strand, within PGA1_c07100 at 730.037 kb on + strand, within PGA1_c07100 at 730.085 kb on + strand, within PGA1_c07100 at 730.087 kb on + strand, within PGA1_c07100 at 730.087 kb on + strand, within PGA1_c07100 at 730.087 kb on + strand, within PGA1_c07100 at 730.095 kb on - strand, within PGA1_c07100 at 730.095 kb on - strand, within PGA1_c07100 at 730.095 kb on - strand, within PGA1_c07100 at 730.104 kb on + strand, within PGA1_c07100 at 730.127 kb on + strand, within PGA1_c07100 at 730.135 kb on - strand, within PGA1_c07100 at 730.173 kb on - strand, within PGA1_c07100 at 730.192 kb on + strand, within PGA1_c07100 at 730.219 kb on + strand, within PGA1_c07100 at 730.219 kb on + strand, within PGA1_c07100 at 730.324 kb on + strand, within PGA1_c07100 at 730.332 kb on - strand, within PGA1_c07100 at 730.349 kb on + strand, within PGA1_c07100 at 730.354 kb on + strand, within PGA1_c07100 at 730.357 kb on - strand, within PGA1_c07100 at 730.357 kb on - strand, within PGA1_c07100 at 730.357 kb on - strand, within PGA1_c07100 at 730.357 kb on - strand, within PGA1_c07100 at 730.419 kb on + strand, within PGA1_c07100 at 730.426 kb on - strand, within PGA1_c07100 at 730.452 kb on - strand, within PGA1_c07100 at 730.463 kb on - strand, within PGA1_c07100 at 730.463 kb on - strand, within PGA1_c07100 at 730.478 kb on - strand, within PGA1_c07100 at 730.517 kb on - strand, within PGA1_c07100 at 730.569 kb on - strand, within PGA1_c07100 at 730.623 kb on - strand, within PGA1_c07100 at 730.687 kb on + strand, within PGA1_c07100 at 730.762 kb on + strand, within PGA1_c07100 at 730.826 kb on - strand, within PGA1_c07100 at 730.873 kb on + strand at 730.876 kb on - strand at 730.910 kb on - strand at 730.928 kb on + strand at 730.936 kb on - strand at 730.936 kb on - strand at 730.936 kb on - strand at 730.961 kb on + strand at 730.969 kb on - strand at 731.013 kb on + strand at 731.013 kb on + strand at 731.021 kb on - strand at 731.021 kb on - strand at 731.021 kb on - strand at 731.045 kb on - strand at 731.073 kb on + strand at 731.073 kb on + strand at 731.073 kb on + strand at 731.073 kb on + strand at 731.073 kb on - strand at 731.081 kb on - strand at 731.081 kb on - strand at 731.210 kb on + strand at 731.330 kb on - strand, within PGA1_c07110 at 731.353 kb on + strand, within PGA1_c07110 at 731.353 kb on + strand, within PGA1_c07110 at 731.354 kb on + strand, within PGA1_c07110 at 731.354 kb on + strand, within PGA1_c07110 at 731.354 kb on + strand, within PGA1_c07110 at 731.361 kb on - strand, within PGA1_c07110 at 731.362 kb on - strand, within PGA1_c07110 at 731.362 kb on - strand, within PGA1_c07110 at 731.362 kb on - strand, within PGA1_c07110 at 731.362 kb on - strand, within PGA1_c07110 at 731.362 kb on - strand, within PGA1_c07110 at 731.367 kb on + strand, within PGA1_c07110 at 731.435 kb on - strand, within PGA1_c07110 at 731.480 kb on + strand, within PGA1_c07110 at 731.510 kb on + strand, within PGA1_c07110 at 731.524 kb on + strand, within PGA1_c07110 at 731.586 kb on + strand, within PGA1_c07110 at 731.632 kb on - strand, within PGA1_c07110 at 731.638 kb on + strand, within PGA1_c07110 at 731.869 kb on + strand, within PGA1_c07110 at 731.876 kb on + strand, within PGA1_c07110 at 731.876 kb on + strand, within PGA1_c07110 at 731.884 kb on - strand, within PGA1_c07110
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. D-Cycloserine 0.0625 mg/ml remove 727,713 + -0.2 727,721 - -1.7 727,830 - -0.4 727,839 + -0.2 727,958 - PGA1_c07090 0.22 -3.6 727,992 - PGA1_c07090 0.30 +1.6 728,045 - PGA1_c07090 0.42 -0.9 728,086 + PGA1_c07090 0.52 +1.2 728,111 + PGA1_c07090 0.57 -0.5 728,111 + PGA1_c07090 0.57 -4.4 728,111 + PGA1_c07090 0.57 -0.1 728,111 + PGA1_c07090 0.57 -3.3 728,111 + PGA1_c07090 0.57 +0.9 728,111 + PGA1_c07090 0.57 +1.9 728,220 - PGA1_c07090 0.83 -0.5 728,307 - -1.1 728,342 + +0.6 728,347 + -0.7 728,349 + -0.1 728,349 + +1.8 728,349 + -0.6 728,357 - -1.2 728,466 - -0.1 728,510 + -0.7 728,512 - -0.9 728,513 - -0.4 728,515 + +0.0 728,562 + +1.0 728,562 + +0.9 728,569 + -1.3 728,570 - +0.5 728,570 - +1.2 728,570 - -3.7 728,577 - -2.1 728,602 + +0.4 728,624 + +1.3 728,634 + +1.0 728,639 + -1.7 728,664 - -0.7 728,674 - -1.9 728,685 + +0.2 728,698 - +0.2 728,698 - -1.4 728,748 - +0.8 728,753 + -0.7 728,756 - -1.7 728,763 + +3.2 728,843 - -0.8 728,847 + +0.5 728,847 + -1.2 728,855 - -0.4 728,855 - +1.2 728,930 + +0.4 729,014 + PGA1_c07100 0.13 -2.0 729,022 - PGA1_c07100 0.13 -0.6 729,022 - PGA1_c07100 0.13 +0.9 729,029 + PGA1_c07100 0.14 -0.9 729,052 - PGA1_c07100 0.15 -1.9 729,062 - PGA1_c07100 0.15 +0.1 729,078 - PGA1_c07100 0.16 -2.3 729,095 + PGA1_c07100 0.17 -1.2 729,125 - PGA1_c07100 0.18 -0.8 729,197 - PGA1_c07100 0.21 -1.3 729,202 - PGA1_c07100 0.21 -0.7 729,242 - PGA1_c07100 0.23 -1.0 729,253 + PGA1_c07100 0.23 +0.2 729,315 - PGA1_c07100 0.26 -2.9 729,489 - PGA1_c07100 0.33 +1.1 729,495 + PGA1_c07100 0.34 +0.1 729,512 + PGA1_c07100 0.34 +1.2 729,529 - PGA1_c07100 0.35 +0.2 729,532 + PGA1_c07100 0.35 -1.1 729,552 + PGA1_c07100 0.36 -1.2 729,566 - PGA1_c07100 0.37 -0.8 729,566 - PGA1_c07100 0.37 +0.9 729,575 + PGA1_c07100 0.37 +0.4 729,592 + PGA1_c07100 0.38 +0.7 729,602 + PGA1_c07100 0.38 -1.5 729,610 - PGA1_c07100 0.38 +0.4 729,624 - PGA1_c07100 0.39 -2.9 729,640 + PGA1_c07100 0.40 +0.9 729,672 - PGA1_c07100 0.41 -0.1 729,697 + PGA1_c07100 0.42 +0.3 729,697 + PGA1_c07100 0.42 -1.1 729,705 - PGA1_c07100 0.42 +0.4 729,705 - PGA1_c07100 0.42 -1.7 729,751 - PGA1_c07100 0.44 +0.4 729,822 + PGA1_c07100 0.47 +0.4 729,906 - PGA1_c07100 0.51 -1.1 729,932 + PGA1_c07100 0.52 +0.7 730,037 + PGA1_c07100 0.56 -0.6 730,085 + PGA1_c07100 0.59 -0.3 730,087 + PGA1_c07100 0.59 -0.8 730,087 + PGA1_c07100 0.59 -0.9 730,087 + PGA1_c07100 0.59 -0.7 730,095 - PGA1_c07100 0.59 -0.2 730,095 - PGA1_c07100 0.59 -0.7 730,095 - PGA1_c07100 0.59 +0.0 730,104 + PGA1_c07100 0.59 +0.2 730,127 + PGA1_c07100 0.60 -0.3 730,135 - PGA1_c07100 0.61 -0.6 730,173 - PGA1_c07100 0.62 -0.1 730,192 + PGA1_c07100 0.63 -0.9 730,219 + PGA1_c07100 0.64 -0.7 730,219 + PGA1_c07100 0.64 -0.6 730,324 + PGA1_c07100 0.69 -0.3 730,332 - PGA1_c07100 0.69 -0.5 730,349 + PGA1_c07100 0.70 +0.5 730,354 + PGA1_c07100 0.70 +0.2 730,357 - PGA1_c07100 0.70 -2.2 730,357 - PGA1_c07100 0.70 -2.9 730,357 - PGA1_c07100 0.70 -0.2 730,357 - PGA1_c07100 0.70 -1.2 730,419 + PGA1_c07100 0.73 -2.5 730,426 - PGA1_c07100 0.73 +1.0 730,452 - PGA1_c07100 0.74 +1.3 730,463 - PGA1_c07100 0.75 -0.4 730,463 - PGA1_c07100 0.75 +0.7 730,478 - PGA1_c07100 0.75 -0.1 730,517 - PGA1_c07100 0.77 +1.8 730,569 - PGA1_c07100 0.79 -1.1 730,623 - PGA1_c07100 0.81 +1.6 730,687 + PGA1_c07100 0.84 -1.2 730,762 + PGA1_c07100 0.87 +0.8 730,826 - PGA1_c07100 0.90 -1.7 730,873 + -1.8 730,876 - +0.4 730,910 - +0.0 730,928 + +0.8 730,936 - -0.2 730,936 - -0.3 730,936 - -0.3 730,961 + -0.9 730,969 - +0.2 731,013 + -0.1 731,013 + -2.2 731,021 - +0.3 731,021 - +0.7 731,021 - -3.2 731,045 - -2.3 731,073 + +1.3 731,073 + -0.1 731,073 + -0.4 731,073 + +0.0 731,073 - +0.3 731,081 - +0.1 731,081 - -0.2 731,210 + +1.9 731,330 - PGA1_c07110 0.14 +3.1 731,353 + PGA1_c07110 0.15 -0.7 731,353 + PGA1_c07110 0.15 -3.2 731,354 + PGA1_c07110 0.15 +1.2 731,354 + PGA1_c07110 0.15 +0.4 731,354 + PGA1_c07110 0.15 -0.3 731,361 - PGA1_c07110 0.16 +0.0 731,362 - PGA1_c07110 0.16 +1.4 731,362 - PGA1_c07110 0.16 +0.1 731,362 - PGA1_c07110 0.16 +0.5 731,362 - PGA1_c07110 0.16 -0.9 731,362 - PGA1_c07110 0.16 +1.5 731,367 + PGA1_c07110 0.16 +0.3 731,435 - PGA1_c07110 0.21 +0.2 731,480 + PGA1_c07110 0.24 +1.0 731,510 + PGA1_c07110 0.26 -0.1 731,524 + PGA1_c07110 0.26 +0.4 731,586 + PGA1_c07110 0.30 +0.1 731,632 - PGA1_c07110 0.33 -2.0 731,638 + PGA1_c07110 0.34 +0.7 731,869 + PGA1_c07110 0.49 -1.6 731,876 + PGA1_c07110 0.49 -0.3 731,876 + PGA1_c07110 0.49 +0.7 731,884 - PGA1_c07110 0.50 +1.1
Or see this region's nucleotide sequence