Experiment: m.b. D-Cycloserine 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c05850 and PGA1_c05860 overlap by 4 nucleotides PGA1_c05860 and PGA1_c05870 are separated by 99 nucleotides PGA1_c05870 and PGA1_c05880 are separated by 309 nucleotides
PGA1_c05850: PGA1_c05850 - crossover junction endodeoxyribonuclease RuvC, at 599,309 to 599,806
_c05850
PGA1_c05860: PGA1_c05860 - holliday junction ATP-dependent DNA helicase RuvA, at 599,803 to 600,492
_c05860
PGA1_c05870: PGA1_c05870 - holliday junction ATP-dependent DNA helicase RuvB, at 600,592 to 601,641
_c05870
PGA1_c05880: PGA1_c05880 - hypothetical protein, at 601,951 to 602,619
_c05880
Position (kb) 600
601
602 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 599.759 kb on - strand at 599.784 kb on + strand at 599.812 kb on + strand at 599.894 kb on - strand, within PGA1_c05860 at 599.921 kb on + strand, within PGA1_c05860 at 599.941 kb on + strand, within PGA1_c05860 at 599.976 kb on - strand, within PGA1_c05860 at 600.197 kb on - strand, within PGA1_c05860 at 600.203 kb on - strand, within PGA1_c05860 at 600.461 kb on - strand at 600.515 kb on - strand at 600.518 kb on + strand at 600.556 kb on + strand at 600.726 kb on - strand, within PGA1_c05870 at 600.881 kb on - strand, within PGA1_c05870 at 600.891 kb on - strand, within PGA1_c05870 at 601.124 kb on - strand, within PGA1_c05870 at 601.351 kb on - strand, within PGA1_c05870 at 601.378 kb on + strand, within PGA1_c05870 at 601.461 kb on - strand, within PGA1_c05870 at 601.593 kb on - strand at 601.595 kb on + strand at 601.595 kb on + strand at 601.603 kb on - strand at 601.603 kb on - strand at 601.686 kb on + strand at 601.723 kb on + strand at 601.770 kb on - strand at 601.770 kb on - strand at 601.780 kb on + strand at 601.780 kb on - strand at 601.803 kb on - strand at 601.877 kb on + strand at 601.910 kb on + strand at 601.910 kb on + strand at 601.918 kb on - strand at 601.986 kb on - strand at 602.118 kb on + strand, within PGA1_c05880 at 602.158 kb on - strand, within PGA1_c05880 at 602.177 kb on + strand, within PGA1_c05880 at 602.195 kb on - strand, within PGA1_c05880 at 602.276 kb on - strand, within PGA1_c05880 at 602.338 kb on + strand, within PGA1_c05880 at 602.383 kb on + strand, within PGA1_c05880 at 602.383 kb on + strand, within PGA1_c05880 at 602.405 kb on - strand, within PGA1_c05880 at 602.500 kb on + strand, within PGA1_c05880 at 602.548 kb on - strand, within PGA1_c05880 at 602.613 kb on + strand at 602.627 kb on + strand at 602.634 kb on + strand at 602.634 kb on + strand at 602.634 kb on + strand at 602.634 kb on + strand at 602.634 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. D-Cycloserine 0.0625 mg/ml remove 599,759 - -3.9 599,784 + -0.8 599,812 + -2.4 599,894 - PGA1_c05860 0.13 -1.2 599,921 + PGA1_c05860 0.17 -2.2 599,941 + PGA1_c05860 0.20 -0.2 599,976 - PGA1_c05860 0.25 -1.6 600,197 - PGA1_c05860 0.57 -3.2 600,203 - PGA1_c05860 0.58 -0.5 600,461 - -1.2 600,515 - -2.4 600,518 + -1.5 600,556 + +2.1 600,726 - PGA1_c05870 0.13 -1.8 600,881 - PGA1_c05870 0.28 -1.8 600,891 - PGA1_c05870 0.28 -2.0 601,124 - PGA1_c05870 0.51 -1.8 601,351 - PGA1_c05870 0.72 -3.9 601,378 + PGA1_c05870 0.75 -1.2 601,461 - PGA1_c05870 0.83 -2.0 601,593 - -0.2 601,595 + -2.4 601,595 + +1.4 601,603 - -0.2 601,603 - -1.2 601,686 + -0.6 601,723 + -0.4 601,770 - -1.6 601,770 - -0.0 601,780 + +1.6 601,780 - -0.1 601,803 - +0.8 601,877 + +0.4 601,910 + +0.4 601,910 + +0.4 601,918 - +0.8 601,986 - -0.2 602,118 + PGA1_c05880 0.25 +0.8 602,158 - PGA1_c05880 0.31 +1.2 602,177 + PGA1_c05880 0.34 +1.3 602,195 - PGA1_c05880 0.36 +0.6 602,276 - PGA1_c05880 0.49 +0.0 602,338 + PGA1_c05880 0.58 +0.8 602,383 + PGA1_c05880 0.65 +0.8 602,383 + PGA1_c05880 0.65 +1.1 602,405 - PGA1_c05880 0.68 +1.1 602,500 + PGA1_c05880 0.82 +0.1 602,548 - PGA1_c05880 0.89 -0.5 602,613 + -0.1 602,627 + -0.0 602,634 + -0.4 602,634 + +0.1 602,634 + +0.8 602,634 + +0.8 602,634 + -0.2
Or see this region's nucleotide sequence