Experiment: m.b. D-Cycloserine 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c05830 and PGA1_c05840 are separated by 206 nucleotides PGA1_c05840 and PGA1_c05850 are separated by 361 nucleotides PGA1_c05850 and PGA1_c05860 overlap by 4 nucleotides PGA1_c05860 and PGA1_c05870 are separated by 99 nucleotides
PGA1_c05830: PGA1_c05830 - hypothetical protein, at 598,178 to 598,522
_c05830
PGA1_c05840: PGA1_c05840 - Uncharacterized conserved small protein, at 598,729 to 598,947
_c05840
PGA1_c05850: PGA1_c05850 - crossover junction endodeoxyribonuclease RuvC, at 599,309 to 599,806
_c05850
PGA1_c05860: PGA1_c05860 - holliday junction ATP-dependent DNA helicase RuvA, at 599,803 to 600,492
_c05860
PGA1_c05870: PGA1_c05870 - holliday junction ATP-dependent DNA helicase RuvB, at 600,592 to 601,641
_c05870
Position (kb)
599
600 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 598.360 kb on + strand, within PGA1_c05830 at 598.360 kb on + strand, within PGA1_c05830 at 598.368 kb on - strand, within PGA1_c05830 at 598.368 kb on - strand, within PGA1_c05830 at 598.369 kb on - strand, within PGA1_c05830 at 598.426 kb on + strand, within PGA1_c05830 at 598.466 kb on - strand, within PGA1_c05830 at 598.494 kb on - strand at 598.506 kb on + strand at 598.509 kb on - strand at 598.509 kb on - strand at 598.509 kb on - strand at 598.514 kb on - strand at 598.576 kb on + strand at 598.586 kb on + strand at 598.586 kb on + strand at 598.594 kb on - strand at 598.594 kb on - strand at 598.594 kb on - strand at 598.594 kb on - strand at 598.605 kb on + strand at 598.613 kb on - strand at 598.633 kb on - strand at 598.680 kb on + strand at 598.712 kb on + strand at 598.739 kb on + strand at 598.774 kb on + strand, within PGA1_c05840 at 598.782 kb on - strand, within PGA1_c05840 at 598.782 kb on - strand, within PGA1_c05840 at 598.787 kb on - strand, within PGA1_c05840 at 598.787 kb on - strand, within PGA1_c05840 at 598.787 kb on - strand, within PGA1_c05840 at 598.943 kb on + strand at 599.009 kb on - strand at 599.103 kb on + strand at 599.111 kb on - strand at 599.144 kb on - strand at 599.291 kb on + strand at 599.296 kb on + strand at 599.304 kb on - strand at 599.309 kb on - strand at 599.309 kb on - strand at 599.357 kb on - strand at 599.562 kb on + strand, within PGA1_c05850 at 599.562 kb on - strand, within PGA1_c05850 at 599.577 kb on + strand, within PGA1_c05850 at 599.759 kb on - strand at 599.784 kb on + strand at 599.812 kb on + strand at 599.894 kb on - strand, within PGA1_c05860 at 599.921 kb on + strand, within PGA1_c05860 at 599.941 kb on + strand, within PGA1_c05860 at 599.976 kb on - strand, within PGA1_c05860 at 600.197 kb on - strand, within PGA1_c05860 at 600.203 kb on - strand, within PGA1_c05860 at 600.461 kb on - strand at 600.515 kb on - strand at 600.518 kb on + strand at 600.556 kb on + strand at 600.726 kb on - strand, within PGA1_c05870
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. D-Cycloserine 0.0625 mg/ml remove 598,360 + PGA1_c05830 0.53 -0.4 598,360 + PGA1_c05830 0.53 +1.1 598,368 - PGA1_c05830 0.55 -0.2 598,368 - PGA1_c05830 0.55 -2.8 598,369 - PGA1_c05830 0.55 +1.3 598,426 + PGA1_c05830 0.72 +1.5 598,466 - PGA1_c05830 0.83 +0.7 598,494 - +0.8 598,506 + -2.9 598,509 - +0.6 598,509 - +0.5 598,509 - +1.6 598,514 - -1.6 598,576 + +0.8 598,586 + +1.1 598,586 + -0.5 598,594 - -0.5 598,594 - +0.1 598,594 - -0.1 598,594 - -2.0 598,605 + -1.6 598,613 - +0.6 598,633 - -0.2 598,680 + +0.2 598,712 + +0.8 598,739 + -0.2 598,774 + PGA1_c05840 0.21 -1.0 598,782 - PGA1_c05840 0.24 +1.9 598,782 - PGA1_c05840 0.24 +0.6 598,787 - PGA1_c05840 0.26 +1.0 598,787 - PGA1_c05840 0.26 -2.0 598,787 - PGA1_c05840 0.26 -1.0 598,943 + -0.8 599,009 - +1.8 599,103 + +0.4 599,111 - -0.7 599,144 - -2.2 599,291 + +0.1 599,296 + +0.0 599,304 - -0.2 599,309 - -0.8 599,309 - -1.4 599,357 - -0.2 599,562 + PGA1_c05850 0.51 +0.8 599,562 - PGA1_c05850 0.51 -0.8 599,577 + PGA1_c05850 0.54 -1.8 599,759 - -3.9 599,784 + -0.8 599,812 + -2.4 599,894 - PGA1_c05860 0.13 -1.2 599,921 + PGA1_c05860 0.17 -2.2 599,941 + PGA1_c05860 0.20 -0.2 599,976 - PGA1_c05860 0.25 -1.6 600,197 - PGA1_c05860 0.57 -3.2 600,203 - PGA1_c05860 0.58 -0.5 600,461 - -1.2 600,515 - -2.4 600,518 + -1.5 600,556 + +2.1 600,726 - PGA1_c05870 0.13 -1.8
Or see this region's nucleotide sequence