Experiment: m.b. D-Cycloserine 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c03780 and PGA1_c03790 are separated by 212 nucleotides PGA1_c03790 and PGA1_c03800 are separated by 507 nucleotides PGA1_c03800 and PGA1_c03810 are separated by 105 nucleotides
PGA1_c03780: PGA1_c03780 - succinate dehydrogenase iron-sulfur subunit, at 363,120 to 363,899
_c03780
PGA1_c03790: PGA1_c03790 - hypothetical protein, at 364,112 to 364,303
_c03790
PGA1_c03800: PGA1_c03800 - putative lysine/ornithine decarboxylase, at 364,811 to 365,992
_c03800
PGA1_c03810: PGA1_c03810 - Uncharacterized conserved protein, at 366,098 to 366,973
_c03810
Position (kb)
364
365
366 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 363.906 kb on - strand at 363.928 kb on - strand at 363.933 kb on + strand at 363.933 kb on + strand at 363.970 kb on + strand at 364.001 kb on + strand at 364.006 kb on + strand at 364.083 kb on - strand at 364.109 kb on + strand at 364.175 kb on - strand, within PGA1_c03790 at 364.176 kb on - strand, within PGA1_c03790 at 364.213 kb on + strand, within PGA1_c03790 at 364.213 kb on + strand, within PGA1_c03790 at 364.213 kb on + strand, within PGA1_c03790 at 364.213 kb on + strand, within PGA1_c03790 at 364.213 kb on + strand, within PGA1_c03790 at 364.213 kb on + strand, within PGA1_c03790 at 364.213 kb on + strand, within PGA1_c03790 at 364.213 kb on + strand, within PGA1_c03790 at 364.219 kb on + strand, within PGA1_c03790 at 364.221 kb on - strand, within PGA1_c03790 at 364.221 kb on - strand, within PGA1_c03790 at 364.221 kb on - strand, within PGA1_c03790 at 364.221 kb on - strand, within PGA1_c03790 at 364.221 kb on - strand, within PGA1_c03790 at 364.221 kb on - strand, within PGA1_c03790 at 364.221 kb on - strand, within PGA1_c03790 at 364.221 kb on - strand, within PGA1_c03790 at 364.292 kb on + strand at 364.350 kb on - strand at 364.441 kb on - strand at 364.441 kb on - strand at 364.483 kb on - strand at 364.488 kb on + strand at 364.488 kb on - strand at 364.488 kb on - strand at 364.528 kb on - strand at 364.598 kb on + strand at 364.601 kb on - strand at 364.606 kb on - strand at 364.677 kb on + strand at 364.724 kb on + strand at 364.731 kb on + strand at 364.733 kb on - strand at 364.882 kb on + strand at 364.882 kb on + strand at 364.882 kb on + strand at 364.882 kb on + strand at 364.890 kb on - strand at 364.890 kb on - strand at 364.890 kb on - strand at 364.901 kb on + strand at 364.970 kb on + strand, within PGA1_c03800 at 364.970 kb on + strand, within PGA1_c03800 at 364.970 kb on + strand, within PGA1_c03800 at 364.970 kb on + strand, within PGA1_c03800 at 364.970 kb on + strand, within PGA1_c03800 at 364.970 kb on + strand, within PGA1_c03800 at 364.970 kb on + strand, within PGA1_c03800 at 364.970 kb on + strand, within PGA1_c03800 at 364.970 kb on + strand, within PGA1_c03800 at 364.970 kb on + strand, within PGA1_c03800 at 364.970 kb on + strand, within PGA1_c03800 at 364.970 kb on + strand, within PGA1_c03800 at 364.970 kb on + strand, within PGA1_c03800 at 364.970 kb on + strand, within PGA1_c03800 at 364.978 kb on - strand, within PGA1_c03800 at 364.978 kb on - strand, within PGA1_c03800 at 364.978 kb on - strand, within PGA1_c03800 at 364.978 kb on - strand, within PGA1_c03800 at 364.978 kb on - strand, within PGA1_c03800 at 365.008 kb on - strand, within PGA1_c03800 at 365.041 kb on - strand, within PGA1_c03800 at 365.129 kb on + strand, within PGA1_c03800 at 365.215 kb on + strand, within PGA1_c03800 at 365.248 kb on + strand, within PGA1_c03800 at 365.308 kb on + strand, within PGA1_c03800 at 365.321 kb on + strand, within PGA1_c03800 at 365.327 kb on + strand, within PGA1_c03800 at 365.327 kb on + strand, within PGA1_c03800 at 365.328 kb on + strand, within PGA1_c03800 at 365.335 kb on - strand, within PGA1_c03800 at 365.335 kb on - strand, within PGA1_c03800 at 365.355 kb on + strand, within PGA1_c03800 at 365.355 kb on + strand, within PGA1_c03800 at 365.355 kb on + strand, within PGA1_c03800 at 365.355 kb on + strand, within PGA1_c03800 at 365.363 kb on - strand, within PGA1_c03800 at 365.365 kb on + strand, within PGA1_c03800 at 365.403 kb on + strand, within PGA1_c03800 at 365.411 kb on - strand, within PGA1_c03800 at 365.438 kb on - strand, within PGA1_c03800 at 365.449 kb on + strand, within PGA1_c03800 at 365.449 kb on + strand, within PGA1_c03800 at 365.449 kb on + strand, within PGA1_c03800 at 365.449 kb on + strand, within PGA1_c03800 at 365.449 kb on + strand, within PGA1_c03800 at 365.457 kb on - strand, within PGA1_c03800 at 365.457 kb on - strand, within PGA1_c03800 at 365.457 kb on - strand, within PGA1_c03800 at 365.457 kb on - strand, within PGA1_c03800 at 365.457 kb on - strand, within PGA1_c03800 at 365.526 kb on + strand, within PGA1_c03800 at 365.540 kb on + strand, within PGA1_c03800 at 365.671 kb on - strand, within PGA1_c03800 at 365.701 kb on + strand, within PGA1_c03800 at 365.701 kb on + strand, within PGA1_c03800 at 365.706 kb on + strand, within PGA1_c03800 at 365.740 kb on + strand, within PGA1_c03800 at 365.748 kb on - strand, within PGA1_c03800 at 365.778 kb on + strand, within PGA1_c03800 at 365.778 kb on + strand, within PGA1_c03800 at 365.790 kb on + strand, within PGA1_c03800 at 365.790 kb on + strand, within PGA1_c03800 at 365.817 kb on - strand, within PGA1_c03800 at 365.838 kb on + strand, within PGA1_c03800 at 365.843 kb on + strand, within PGA1_c03800 at 365.846 kb on - strand, within PGA1_c03800 at 365.846 kb on - strand, within PGA1_c03800 at 365.853 kb on + strand, within PGA1_c03800 at 365.981 kb on + strand at 365.982 kb on - strand at 365.993 kb on + strand at 366.018 kb on + strand at 366.026 kb on - strand at 366.079 kb on + strand at 366.129 kb on - strand at 366.131 kb on + strand at 366.139 kb on + strand at 366.157 kb on - strand at 366.223 kb on - strand, within PGA1_c03810 at 366.232 kb on + strand, within PGA1_c03810 at 366.232 kb on + strand, within PGA1_c03810 at 366.232 kb on + strand, within PGA1_c03810 at 366.232 kb on + strand, within PGA1_c03810 at 366.240 kb on - strand, within PGA1_c03810 at 366.240 kb on - strand, within PGA1_c03810 at 366.267 kb on - strand, within PGA1_c03810 at 366.328 kb on + strand, within PGA1_c03810 at 366.350 kb on + strand, within PGA1_c03810 at 366.350 kb on + strand, within PGA1_c03810 at 366.358 kb on - strand, within PGA1_c03810 at 366.358 kb on - strand, within PGA1_c03810 at 366.358 kb on - strand, within PGA1_c03810 at 366.369 kb on + strand, within PGA1_c03810 at 366.369 kb on + strand, within PGA1_c03810 at 366.369 kb on + strand, within PGA1_c03810 at 366.369 kb on + strand, within PGA1_c03810 at 366.369 kb on + strand, within PGA1_c03810 at 366.372 kb on - strand, within PGA1_c03810 at 366.377 kb on - strand, within PGA1_c03810 at 366.377 kb on - strand, within PGA1_c03810 at 366.377 kb on - strand, within PGA1_c03810 at 366.377 kb on - strand, within PGA1_c03810 at 366.415 kb on + strand, within PGA1_c03810 at 366.420 kb on + strand, within PGA1_c03810 at 366.420 kb on + strand, within PGA1_c03810 at 366.428 kb on - strand, within PGA1_c03810 at 366.432 kb on + strand, within PGA1_c03810 at 366.434 kb on + strand, within PGA1_c03810 at 366.434 kb on + strand, within PGA1_c03810 at 366.440 kb on - strand, within PGA1_c03810 at 366.442 kb on - strand, within PGA1_c03810 at 366.443 kb on + strand, within PGA1_c03810 at 366.443 kb on + strand, within PGA1_c03810 at 366.443 kb on + strand, within PGA1_c03810 at 366.447 kb on - strand, within PGA1_c03810 at 366.453 kb on - strand, within PGA1_c03810 at 366.458 kb on - strand, within PGA1_c03810 at 366.458 kb on - strand, within PGA1_c03810 at 366.497 kb on + strand, within PGA1_c03810 at 366.497 kb on + strand, within PGA1_c03810 at 366.497 kb on + strand, within PGA1_c03810 at 366.497 kb on + strand, within PGA1_c03810 at 366.497 kb on + strand, within PGA1_c03810 at 366.541 kb on + strand, within PGA1_c03810 at 366.612 kb on - strand, within PGA1_c03810 at 366.620 kb on + strand, within PGA1_c03810 at 366.620 kb on + strand, within PGA1_c03810 at 366.623 kb on - strand, within PGA1_c03810 at 366.628 kb on - strand, within PGA1_c03810 at 366.646 kb on + strand, within PGA1_c03810 at 366.731 kb on + strand, within PGA1_c03810 at 366.745 kb on - strand, within PGA1_c03810 at 366.753 kb on + strand, within PGA1_c03810 at 366.757 kb on + strand, within PGA1_c03810 at 366.810 kb on - strand, within PGA1_c03810 at 366.810 kb on - strand, within PGA1_c03810 at 366.845 kb on + strand, within PGA1_c03810 at 366.845 kb on + strand, within PGA1_c03810 at 366.853 kb on - strand, within PGA1_c03810 at 366.853 kb on - strand, within PGA1_c03810 at 366.867 kb on + strand, within PGA1_c03810 at 366.891 kb on - strand at 366.892 kb on + strand at 366.906 kb on + strand at 366.911 kb on + strand at 366.911 kb on + strand at 366.919 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. D-Cycloserine 0.0625 mg/ml remove 363,906 - +1.9 363,928 - +0.2 363,933 + +0.2 363,933 + +1.5 363,970 + -0.1 364,001 + -1.2 364,006 + +1.2 364,083 - -2.0 364,109 + -0.8 364,175 - PGA1_c03790 0.33 -2.8 364,176 - PGA1_c03790 0.33 -1.1 364,213 + PGA1_c03790 0.53 -1.1 364,213 + PGA1_c03790 0.53 -0.8 364,213 + PGA1_c03790 0.53 +0.1 364,213 + PGA1_c03790 0.53 -0.3 364,213 + PGA1_c03790 0.53 -1.7 364,213 + PGA1_c03790 0.53 -0.2 364,213 + PGA1_c03790 0.53 -1.1 364,213 + PGA1_c03790 0.53 -0.9 364,219 + PGA1_c03790 0.56 -0.4 364,221 - PGA1_c03790 0.57 +0.4 364,221 - PGA1_c03790 0.57 +0.1 364,221 - PGA1_c03790 0.57 -1.1 364,221 - PGA1_c03790 0.57 -1.7 364,221 - PGA1_c03790 0.57 -0.3 364,221 - PGA1_c03790 0.57 +1.1 364,221 - PGA1_c03790 0.57 +0.2 364,221 - PGA1_c03790 0.57 -0.8 364,292 + +0.7 364,350 - +0.6 364,441 - +0.3 364,441 - +0.5 364,483 - -1.8 364,488 + -3.4 364,488 - -0.0 364,488 - +0.4 364,528 - +0.6 364,598 + +0.6 364,601 - -1.1 364,606 - -0.2 364,677 + -0.7 364,724 + -2.5 364,731 + +0.4 364,733 - -2.1 364,882 + -1.1 364,882 + -1.5 364,882 + -1.3 364,882 + +0.8 364,890 - +1.0 364,890 - +0.8 364,890 - +0.2 364,901 + -1.0 364,970 + PGA1_c03800 0.13 -1.4 364,970 + PGA1_c03800 0.13 -0.5 364,970 + PGA1_c03800 0.13 -0.0 364,970 + PGA1_c03800 0.13 +0.8 364,970 + PGA1_c03800 0.13 -0.5 364,970 + PGA1_c03800 0.13 -0.9 364,970 + PGA1_c03800 0.13 -0.5 364,970 + PGA1_c03800 0.13 +0.5 364,970 + PGA1_c03800 0.13 -0.4 364,970 + PGA1_c03800 0.13 -0.2 364,970 + PGA1_c03800 0.13 +0.5 364,970 + PGA1_c03800 0.13 -0.2 364,970 + PGA1_c03800 0.13 -3.8 364,970 + PGA1_c03800 0.13 +0.2 364,978 - PGA1_c03800 0.14 +1.0 364,978 - PGA1_c03800 0.14 +0.8 364,978 - PGA1_c03800 0.14 +0.6 364,978 - PGA1_c03800 0.14 +0.6 364,978 - PGA1_c03800 0.14 -0.8 365,008 - PGA1_c03800 0.17 +1.1 365,041 - PGA1_c03800 0.19 -1.5 365,129 + PGA1_c03800 0.27 +0.0 365,215 + PGA1_c03800 0.34 -0.4 365,248 + PGA1_c03800 0.37 +0.6 365,308 + PGA1_c03800 0.42 +0.2 365,321 + PGA1_c03800 0.43 -0.2 365,327 + PGA1_c03800 0.44 -0.9 365,327 + PGA1_c03800 0.44 -2.4 365,328 + PGA1_c03800 0.44 +0.2 365,335 - PGA1_c03800 0.44 -1.8 365,335 - PGA1_c03800 0.44 +1.2 365,355 + PGA1_c03800 0.46 -1.1 365,355 + PGA1_c03800 0.46 -0.2 365,355 + PGA1_c03800 0.46 -1.8 365,355 + PGA1_c03800 0.46 -1.9 365,363 - PGA1_c03800 0.47 +0.0 365,365 + PGA1_c03800 0.47 -2.5 365,403 + PGA1_c03800 0.50 -0.4 365,411 - PGA1_c03800 0.51 -0.5 365,438 - PGA1_c03800 0.53 -2.4 365,449 + PGA1_c03800 0.54 -1.8 365,449 + PGA1_c03800 0.54 +1.2 365,449 + PGA1_c03800 0.54 -0.2 365,449 + PGA1_c03800 0.54 -0.5 365,449 + PGA1_c03800 0.54 +0.2 365,457 - PGA1_c03800 0.55 -0.7 365,457 - PGA1_c03800 0.55 -0.0 365,457 - PGA1_c03800 0.55 +0.3 365,457 - PGA1_c03800 0.55 -0.2 365,457 - PGA1_c03800 0.55 -1.1 365,526 + PGA1_c03800 0.60 -2.4 365,540 + PGA1_c03800 0.62 +1.0 365,671 - PGA1_c03800 0.73 +0.6 365,701 + PGA1_c03800 0.75 +0.4 365,701 + PGA1_c03800 0.75 -0.3 365,706 + PGA1_c03800 0.76 +0.4 365,740 + PGA1_c03800 0.79 -0.2 365,748 - PGA1_c03800 0.79 -0.4 365,778 + PGA1_c03800 0.82 -0.5 365,778 + PGA1_c03800 0.82 -0.8 365,790 + PGA1_c03800 0.83 +0.6 365,790 + PGA1_c03800 0.83 -0.0 365,817 - PGA1_c03800 0.85 -1.2 365,838 + PGA1_c03800 0.87 -1.5 365,843 + PGA1_c03800 0.87 -0.9 365,846 - PGA1_c03800 0.88 -1.2 365,846 - PGA1_c03800 0.88 +1.2 365,853 + PGA1_c03800 0.88 +0.8 365,981 + +0.5 365,982 - -0.2 365,993 + -2.9 366,018 + -0.6 366,026 - -1.0 366,079 + +0.4 366,129 - -0.4 366,131 + -2.5 366,139 + -0.1 366,157 - -1.0 366,223 - PGA1_c03810 0.14 +1.7 366,232 + PGA1_c03810 0.15 +0.6 366,232 + PGA1_c03810 0.15 +0.5 366,232 + PGA1_c03810 0.15 -3.8 366,232 + PGA1_c03810 0.15 -0.0 366,240 - PGA1_c03810 0.16 -1.4 366,240 - PGA1_c03810 0.16 -0.4 366,267 - PGA1_c03810 0.19 +1.1 366,328 + PGA1_c03810 0.26 +1.1 366,350 + PGA1_c03810 0.29 -1.3 366,350 + PGA1_c03810 0.29 -2.8 366,358 - PGA1_c03810 0.30 -0.2 366,358 - PGA1_c03810 0.30 -1.8 366,358 - PGA1_c03810 0.30 +0.3 366,369 + PGA1_c03810 0.31 -0.4 366,369 + PGA1_c03810 0.31 +0.2 366,369 + PGA1_c03810 0.31 -0.3 366,369 + PGA1_c03810 0.31 -0.1 366,369 + PGA1_c03810 0.31 +1.1 366,372 - PGA1_c03810 0.31 -2.2 366,377 - PGA1_c03810 0.32 -0.3 366,377 - PGA1_c03810 0.32 -0.7 366,377 - PGA1_c03810 0.32 +0.5 366,377 - PGA1_c03810 0.32 -0.3 366,415 + PGA1_c03810 0.36 -2.1 366,420 + PGA1_c03810 0.37 +0.6 366,420 + PGA1_c03810 0.37 -0.2 366,428 - PGA1_c03810 0.38 +0.5 366,432 + PGA1_c03810 0.38 -0.4 366,434 + PGA1_c03810 0.38 -0.7 366,434 + PGA1_c03810 0.38 -1.8 366,440 - PGA1_c03810 0.39 -0.4 366,442 - PGA1_c03810 0.39 -0.4 366,443 + PGA1_c03810 0.39 -0.1 366,443 + PGA1_c03810 0.39 -2.9 366,443 + PGA1_c03810 0.39 +0.5 366,447 - PGA1_c03810 0.40 -0.8 366,453 - PGA1_c03810 0.41 +0.2 366,458 - PGA1_c03810 0.41 +0.2 366,458 - PGA1_c03810 0.41 +1.2 366,497 + PGA1_c03810 0.46 -1.4 366,497 + PGA1_c03810 0.46 -1.2 366,497 + PGA1_c03810 0.46 -1.8 366,497 + PGA1_c03810 0.46 -1.9 366,497 + PGA1_c03810 0.46 -0.0 366,541 + PGA1_c03810 0.51 -0.9 366,612 - PGA1_c03810 0.59 +0.6 366,620 + PGA1_c03810 0.60 -1.0 366,620 + PGA1_c03810 0.60 +0.9 366,623 - PGA1_c03810 0.60 +0.3 366,628 - PGA1_c03810 0.61 -0.2 366,646 + PGA1_c03810 0.63 +1.1 366,731 + PGA1_c03810 0.72 -2.2 366,745 - PGA1_c03810 0.74 +1.1 366,753 + PGA1_c03810 0.75 -0.0 366,757 + PGA1_c03810 0.75 -2.7 366,810 - PGA1_c03810 0.81 +0.7 366,810 - PGA1_c03810 0.81 +0.1 366,845 + PGA1_c03810 0.85 -0.6 366,845 + PGA1_c03810 0.85 +0.3 366,853 - PGA1_c03810 0.86 -0.5 366,853 - PGA1_c03810 0.86 +1.1 366,867 + PGA1_c03810 0.88 -2.2 366,891 - +0.2 366,892 + -0.2 366,906 + +1.6 366,911 + -1.3 366,911 + +1.0 366,919 - +0.4
Or see this region's nucleotide sequence