Experiment: m.b. D-Cycloserine 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c03410 and PGA1_c03420 are separated by 121 nucleotides PGA1_c03420 and PGA1_c03430 overlap by 8 nucleotides PGA1_c03430 and PGA1_c03440 are separated by 119 nucleotides
PGA1_c03410: PGA1_c03410 - EAL domain-containing protein, at 327,954 to 328,796
_c03410
PGA1_c03420: PGA1_c03420 - putative C-5 sterol desaturase, at 328,918 to 329,847
_c03420
PGA1_c03430: PGA1_c03430 - hypothetical protein, at 329,840 to 330,280
_c03430
PGA1_c03440: PGA1_c03440 - disulfide interchange protein tlpA-like protein, at 330,400 to 330,987
_c03440
Position (kb) 328
329
330 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 327.921 kb on - strand at 327.940 kb on + strand at 327.940 kb on - strand at 327.942 kb on + strand at 327.943 kb on - strand at 327.950 kb on - strand at 327.958 kb on - strand at 327.960 kb on - strand at 327.961 kb on + strand at 327.991 kb on - strand at 328.002 kb on + strand at 328.010 kb on - strand at 328.010 kb on - strand at 328.010 kb on - strand at 328.010 kb on - strand at 328.012 kb on + strand at 328.026 kb on + strand at 328.034 kb on - strand at 328.070 kb on + strand, within PGA1_c03410 at 328.316 kb on + strand, within PGA1_c03410 at 328.321 kb on + strand, within PGA1_c03410 at 328.321 kb on + strand, within PGA1_c03410 at 328.324 kb on - strand, within PGA1_c03410 at 328.347 kb on + strand, within PGA1_c03410 at 328.350 kb on - strand, within PGA1_c03410 at 328.350 kb on - strand, within PGA1_c03410 at 328.441 kb on + strand, within PGA1_c03410 at 328.453 kb on - strand, within PGA1_c03410 at 328.463 kb on + strand, within PGA1_c03410 at 328.502 kb on + strand, within PGA1_c03410 at 328.504 kb on - strand, within PGA1_c03410 at 328.507 kb on + strand, within PGA1_c03410 at 328.517 kb on + strand, within PGA1_c03410 at 328.599 kb on + strand, within PGA1_c03410 at 328.614 kb on - strand, within PGA1_c03410 at 328.616 kb on + strand, within PGA1_c03410 at 328.633 kb on + strand, within PGA1_c03410 at 328.730 kb on + strand at 328.738 kb on - strand at 328.798 kb on + strand at 328.798 kb on + strand at 328.798 kb on + strand at 328.798 kb on + strand at 328.798 kb on + strand at 328.798 kb on + strand at 328.806 kb on - strand at 328.806 kb on - strand at 328.806 kb on - strand at 328.806 kb on - strand at 328.806 kb on - strand at 328.807 kb on - strand at 328.816 kb on + strand at 328.816 kb on + strand at 328.816 kb on + strand at 328.816 kb on + strand at 328.816 kb on + strand at 328.824 kb on - strand at 328.824 kb on - strand at 328.824 kb on - strand at 328.824 kb on - strand at 328.824 kb on - strand at 328.824 kb on - strand at 328.824 kb on - strand at 328.824 kb on - strand at 328.829 kb on - strand at 328.829 kb on - strand at 328.829 kb on - strand at 328.834 kb on - strand at 328.839 kb on - strand at 328.841 kb on + strand at 328.841 kb on + strand at 328.841 kb on + strand at 328.841 kb on + strand at 328.849 kb on - strand at 328.849 kb on - strand at 328.915 kb on + strand at 328.916 kb on - strand at 328.918 kb on - strand at 328.918 kb on - strand at 328.918 kb on - strand at 328.918 kb on - strand at 328.921 kb on + strand at 328.923 kb on - strand at 328.923 kb on - strand at 328.923 kb on - strand at 328.923 kb on - strand at 328.923 kb on - strand at 328.942 kb on - strand at 328.944 kb on + strand at 328.949 kb on + strand at 328.952 kb on - strand at 328.980 kb on + strand at 328.980 kb on + strand at 328.988 kb on - strand at 329.011 kb on + strand, within PGA1_c03420 at 329.099 kb on - strand, within PGA1_c03420 at 329.115 kb on + strand, within PGA1_c03420 at 329.135 kb on - strand, within PGA1_c03420 at 329.139 kb on + strand, within PGA1_c03420 at 329.143 kb on - strand, within PGA1_c03420 at 329.154 kb on - strand, within PGA1_c03420 at 329.169 kb on - strand, within PGA1_c03420 at 329.169 kb on - strand, within PGA1_c03420 at 329.203 kb on - strand, within PGA1_c03420 at 329.217 kb on + strand, within PGA1_c03420 at 329.217 kb on + strand, within PGA1_c03420 at 329.269 kb on + strand, within PGA1_c03420 at 329.274 kb on + strand, within PGA1_c03420 at 329.274 kb on + strand, within PGA1_c03420 at 329.274 kb on + strand, within PGA1_c03420 at 329.284 kb on + strand, within PGA1_c03420 at 329.295 kb on + strand, within PGA1_c03420 at 329.295 kb on + strand, within PGA1_c03420 at 329.303 kb on - strand, within PGA1_c03420 at 329.305 kb on + strand, within PGA1_c03420 at 329.324 kb on + strand, within PGA1_c03420 at 329.344 kb on - strand, within PGA1_c03420 at 329.358 kb on - strand, within PGA1_c03420 at 329.388 kb on + strand, within PGA1_c03420 at 329.404 kb on + strand, within PGA1_c03420 at 329.412 kb on - strand, within PGA1_c03420 at 329.412 kb on - strand, within PGA1_c03420 at 329.441 kb on - strand, within PGA1_c03420 at 329.447 kb on + strand, within PGA1_c03420 at 329.514 kb on + strand, within PGA1_c03420 at 329.520 kb on - strand, within PGA1_c03420 at 329.522 kb on + strand, within PGA1_c03420 at 329.547 kb on + strand, within PGA1_c03420 at 329.552 kb on + strand, within PGA1_c03420 at 329.558 kb on - strand, within PGA1_c03420 at 329.558 kb on - strand, within PGA1_c03420 at 329.606 kb on + strand, within PGA1_c03420 at 329.629 kb on - strand, within PGA1_c03420 at 329.682 kb on + strand, within PGA1_c03420 at 329.714 kb on - strand, within PGA1_c03420 at 329.721 kb on - strand, within PGA1_c03420 at 329.833 kb on - strand at 329.857 kb on - strand at 329.871 kb on - strand at 329.871 kb on - strand at 329.871 kb on - strand at 329.882 kb on - strand at 329.884 kb on + strand, within PGA1_c03430 at 329.892 kb on - strand, within PGA1_c03430 at 329.921 kb on + strand, within PGA1_c03430 at 329.994 kb on + strand, within PGA1_c03430 at 329.994 kb on + strand, within PGA1_c03430 at 330.007 kb on + strand, within PGA1_c03430 at 330.020 kb on - strand, within PGA1_c03430 at 330.022 kb on + strand, within PGA1_c03430 at 330.030 kb on - strand, within PGA1_c03430 at 330.057 kb on + strand, within PGA1_c03430 at 330.065 kb on - strand, within PGA1_c03430 at 330.065 kb on - strand, within PGA1_c03430 at 330.067 kb on + strand, within PGA1_c03430 at 330.082 kb on - strand, within PGA1_c03430 at 330.094 kb on + strand, within PGA1_c03430 at 330.097 kb on - strand, within PGA1_c03430 at 330.103 kb on + strand, within PGA1_c03430 at 330.155 kb on + strand, within PGA1_c03430 at 330.171 kb on - strand, within PGA1_c03430 at 330.172 kb on - strand, within PGA1_c03430 at 330.196 kb on + strand, within PGA1_c03430 at 330.198 kb on - strand, within PGA1_c03430 at 330.250 kb on + strand at 330.287 kb on + strand at 330.332 kb on - strand at 330.333 kb on - strand at 330.367 kb on + strand at 330.375 kb on - strand at 330.385 kb on + strand at 330.393 kb on - strand at 330.402 kb on + strand at 330.402 kb on + strand at 330.405 kb on - strand at 330.801 kb on + strand, within PGA1_c03440
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. D-Cycloserine 0.0625 mg/ml remove 327,921 - -0.0 327,940 + +0.1 327,940 - -0.1 327,942 + -0.1 327,943 - +0.8 327,950 - -0.7 327,958 - +1.5 327,960 - +0.1 327,961 + -1.2 327,991 - +0.4 328,002 + -1.5 328,010 - +2.0 328,010 - -2.0 328,010 - +0.8 328,010 - -1.5 328,012 + -0.9 328,026 + +0.4 328,034 - -1.3 328,070 + PGA1_c03410 0.14 +1.1 328,316 + PGA1_c03410 0.43 -0.3 328,321 + PGA1_c03410 0.44 -3.1 328,321 + PGA1_c03410 0.44 +0.8 328,324 - PGA1_c03410 0.44 -1.1 328,347 + PGA1_c03410 0.47 -2.4 328,350 - PGA1_c03410 0.47 -2.4 328,350 - PGA1_c03410 0.47 -0.9 328,441 + PGA1_c03410 0.58 -1.8 328,453 - PGA1_c03410 0.59 +0.4 328,463 + PGA1_c03410 0.60 +0.4 328,502 + PGA1_c03410 0.65 +0.8 328,504 - PGA1_c03410 0.65 -1.7 328,507 + PGA1_c03410 0.66 -2.4 328,517 + PGA1_c03410 0.67 -3.9 328,599 + PGA1_c03410 0.77 +0.9 328,614 - PGA1_c03410 0.78 -1.4 328,616 + PGA1_c03410 0.79 -1.6 328,633 + PGA1_c03410 0.81 +0.7 328,730 + -0.4 328,738 - -0.6 328,798 + -0.7 328,798 + -0.8 328,798 + -0.3 328,798 + -0.9 328,798 + +1.2 328,798 + -0.6 328,806 - -0.5 328,806 - -1.2 328,806 - +0.0 328,806 - -2.2 328,806 - +0.2 328,807 - +1.1 328,816 + +0.9 328,816 + +0.3 328,816 + -0.0 328,816 + -0.2 328,816 + +0.3 328,824 - -1.3 328,824 - -3.7 328,824 - +0.1 328,824 - +0.1 328,824 - +2.4 328,824 - +1.4 328,824 - +0.3 328,824 - -0.7 328,829 - -2.5 328,829 - -0.1 328,829 - +0.6 328,834 - -0.0 328,839 - +0.1 328,841 + -0.3 328,841 + +0.8 328,841 + -0.9 328,841 + +0.1 328,849 - -1.1 328,849 - -0.7 328,915 + +0.0 328,916 - -0.1 328,918 - -0.5 328,918 - -2.2 328,918 - -2.7 328,918 - -0.8 328,921 + +1.5 328,923 - +1.8 328,923 - +0.7 328,923 - +1.8 328,923 - +1.1 328,923 - -1.0 328,942 - +0.9 328,944 + -1.4 328,949 + -1.4 328,952 - -0.3 328,980 + +0.2 328,980 + +1.5 328,988 - -0.5 329,011 + PGA1_c03420 0.10 -0.8 329,099 - PGA1_c03420 0.19 +0.5 329,115 + PGA1_c03420 0.21 +2.6 329,135 - PGA1_c03420 0.23 -0.6 329,139 + PGA1_c03420 0.24 +0.8 329,143 - PGA1_c03420 0.24 -0.2 329,154 - PGA1_c03420 0.25 +0.4 329,169 - PGA1_c03420 0.27 -3.0 329,169 - PGA1_c03420 0.27 -0.0 329,203 - PGA1_c03420 0.31 +1.2 329,217 + PGA1_c03420 0.32 -2.2 329,217 + PGA1_c03420 0.32 -2.0 329,269 + PGA1_c03420 0.38 +0.1 329,274 + PGA1_c03420 0.38 +1.2 329,274 + PGA1_c03420 0.38 +0.2 329,274 + PGA1_c03420 0.38 +1.6 329,284 + PGA1_c03420 0.39 -1.1 329,295 + PGA1_c03420 0.41 -0.5 329,295 + PGA1_c03420 0.41 +1.5 329,303 - PGA1_c03420 0.41 +1.2 329,305 + PGA1_c03420 0.42 -0.4 329,324 + PGA1_c03420 0.44 +0.3 329,344 - PGA1_c03420 0.46 +1.0 329,358 - PGA1_c03420 0.47 +0.4 329,388 + PGA1_c03420 0.51 +0.5 329,404 + PGA1_c03420 0.52 -0.9 329,412 - PGA1_c03420 0.53 +0.3 329,412 - PGA1_c03420 0.53 +1.0 329,441 - PGA1_c03420 0.56 +0.2 329,447 + PGA1_c03420 0.57 -0.1 329,514 + PGA1_c03420 0.64 -1.0 329,520 - PGA1_c03420 0.65 +0.6 329,522 + PGA1_c03420 0.65 +0.7 329,547 + PGA1_c03420 0.68 +0.6 329,552 + PGA1_c03420 0.68 +0.6 329,558 - PGA1_c03420 0.69 +0.4 329,558 - PGA1_c03420 0.69 -0.3 329,606 + PGA1_c03420 0.74 -0.5 329,629 - PGA1_c03420 0.76 +0.8 329,682 + PGA1_c03420 0.82 -0.0 329,714 - PGA1_c03420 0.86 +0.6 329,721 - PGA1_c03420 0.86 +1.0 329,833 - -1.1 329,857 - -0.9 329,871 - -2.7 329,871 - -1.4 329,871 - -0.9 329,882 - +0.0 329,884 + PGA1_c03430 0.10 +1.4 329,892 - PGA1_c03430 0.12 +0.3 329,921 + PGA1_c03430 0.18 +0.4 329,994 + PGA1_c03430 0.35 +0.3 329,994 + PGA1_c03430 0.35 +2.6 330,007 + PGA1_c03430 0.38 +0.7 330,020 - PGA1_c03430 0.41 -1.2 330,022 + PGA1_c03430 0.41 +0.9 330,030 - PGA1_c03430 0.43 -0.3 330,057 + PGA1_c03430 0.49 +1.2 330,065 - PGA1_c03430 0.51 -0.0 330,065 - PGA1_c03430 0.51 +0.2 330,067 + PGA1_c03430 0.51 +0.3 330,082 - PGA1_c03430 0.55 -0.3 330,094 + PGA1_c03430 0.58 +1.6 330,097 - PGA1_c03430 0.58 -0.5 330,103 + PGA1_c03430 0.60 -0.8 330,155 + PGA1_c03430 0.71 +1.2 330,171 - PGA1_c03430 0.75 -1.5 330,172 - PGA1_c03430 0.75 -0.2 330,196 + PGA1_c03430 0.81 +2.1 330,198 - PGA1_c03430 0.81 +0.8 330,250 + +0.8 330,287 + -1.5 330,332 - +0.4 330,333 - +0.4 330,367 + +1.8 330,375 - -2.8 330,385 + +1.8 330,393 - -0.3 330,402 + -0.1 330,402 + -1.2 330,405 - -0.1 330,801 + PGA1_c03440 0.68 -0.8
Or see this region's nucleotide sequence