Experiment: m.b. D-Cycloserine 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c03230 and PGA1_c03240 are separated by 62 nucleotides PGA1_c03240 and PGA1_c03250 overlap by 4 nucleotides PGA1_c03250 and PGA1_c03260 overlap by 4 nucleotides
PGA1_c03230: PGA1_c03230 - putative formate dehydrogenase, subunit FdhD, at 309,491 to 310,393
_c03230
PGA1_c03240: PGA1_c03240 - probable molybdopterin-guanine dinucleotide biosynthesis protein A, at 310,456 to 311,097
_c03240
PGA1_c03250: PGA1_c03250 - molybdopterin-guanine dinucleotide biosynthesis protein B, at 311,094 to 311,585
_c03250
PGA1_c03260: PGA1_c03260 - hypothetical protein, at 311,582 to 312,394
_c03260
Position (kb) 310
311
312 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 309.459 kb on - strand at 309.460 kb on + strand at 309.460 kb on + strand at 309.468 kb on - strand at 309.468 kb on - strand at 309.468 kb on - strand at 309.468 kb on - strand at 309.515 kb on - strand at 309.520 kb on - strand at 309.739 kb on + strand, within PGA1_c03230 at 309.749 kb on + strand, within PGA1_c03230 at 309.782 kb on + strand, within PGA1_c03230 at 309.827 kb on - strand, within PGA1_c03230 at 309.874 kb on - strand, within PGA1_c03230 at 309.927 kb on + strand, within PGA1_c03230 at 309.927 kb on + strand, within PGA1_c03230 at 309.929 kb on + strand, within PGA1_c03230 at 309.929 kb on + strand, within PGA1_c03230 at 309.937 kb on - strand, within PGA1_c03230 at 309.937 kb on - strand, within PGA1_c03230 at 309.937 kb on - strand, within PGA1_c03230 at 309.986 kb on - strand, within PGA1_c03230 at 309.990 kb on + strand, within PGA1_c03230 at 309.990 kb on + strand, within PGA1_c03230 at 309.998 kb on - strand, within PGA1_c03230 at 309.998 kb on - strand, within PGA1_c03230 at 310.026 kb on + strand, within PGA1_c03230 at 310.045 kb on + strand, within PGA1_c03230 at 310.069 kb on + strand, within PGA1_c03230 at 310.153 kb on + strand, within PGA1_c03230 at 310.158 kb on + strand, within PGA1_c03230 at 310.166 kb on - strand, within PGA1_c03230 at 310.299 kb on - strand, within PGA1_c03230 at 310.348 kb on - strand at 310.440 kb on + strand at 310.639 kb on + strand, within PGA1_c03240 at 310.675 kb on - strand, within PGA1_c03240 at 310.707 kb on - strand, within PGA1_c03240 at 310.721 kb on - strand, within PGA1_c03240 at 310.811 kb on + strand, within PGA1_c03240 at 310.816 kb on + strand, within PGA1_c03240 at 310.922 kb on + strand, within PGA1_c03240 at 310.971 kb on - strand, within PGA1_c03240 at 310.981 kb on - strand, within PGA1_c03240 at 310.990 kb on + strand, within PGA1_c03240 at 310.998 kb on - strand, within PGA1_c03240 at 311.024 kb on - strand, within PGA1_c03240 at 311.024 kb on - strand, within PGA1_c03240 at 311.049 kb on + strand at 311.057 kb on - strand at 311.076 kb on + strand at 311.099 kb on + strand at 311.099 kb on + strand at 311.107 kb on - strand at 311.107 kb on - strand at 311.107 kb on - strand at 311.107 kb on - strand at 311.107 kb on - strand at 311.109 kb on + strand at 311.124 kb on - strand at 311.179 kb on + strand, within PGA1_c03250 at 311.208 kb on + strand, within PGA1_c03250 at 311.221 kb on - strand, within PGA1_c03250 at 311.270 kb on + strand, within PGA1_c03250 at 311.331 kb on + strand, within PGA1_c03250 at 311.376 kb on + strand, within PGA1_c03250 at 311.411 kb on - strand, within PGA1_c03250 at 311.411 kb on - strand, within PGA1_c03250 at 311.420 kb on + strand, within PGA1_c03250 at 311.422 kb on - strand, within PGA1_c03250 at 311.452 kb on + strand, within PGA1_c03250 at 311.499 kb on + strand, within PGA1_c03250 at 311.508 kb on + strand, within PGA1_c03250 at 311.537 kb on - strand at 311.598 kb on + strand at 311.617 kb on - strand at 311.630 kb on + strand at 311.637 kb on + strand at 311.637 kb on + strand at 311.668 kb on - strand, within PGA1_c03260 at 311.707 kb on - strand, within PGA1_c03260 at 311.757 kb on - strand, within PGA1_c03260 at 311.841 kb on + strand, within PGA1_c03260 at 311.871 kb on + strand, within PGA1_c03260 at 311.871 kb on + strand, within PGA1_c03260 at 311.871 kb on + strand, within PGA1_c03260 at 311.913 kb on + strand, within PGA1_c03260 at 311.952 kb on + strand, within PGA1_c03260 at 311.962 kb on + strand, within PGA1_c03260 at 311.975 kb on - strand, within PGA1_c03260 at 311.984 kb on - strand, within PGA1_c03260 at 311.990 kb on + strand, within PGA1_c03260 at 311.998 kb on - strand, within PGA1_c03260 at 312.016 kb on + strand, within PGA1_c03260 at 312.048 kb on + strand, within PGA1_c03260 at 312.050 kb on + strand, within PGA1_c03260 at 312.050 kb on + strand, within PGA1_c03260 at 312.050 kb on + strand, within PGA1_c03260 at 312.050 kb on + strand, within PGA1_c03260 at 312.056 kb on - strand, within PGA1_c03260 at 312.058 kb on - strand, within PGA1_c03260
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. D-Cycloserine 0.0625 mg/ml remove 309,459 - +2.2 309,460 + -1.7 309,460 + -2.2 309,468 - +0.6 309,468 - -0.2 309,468 - -3.1 309,468 - -3.0 309,515 - +0.3 309,520 - +1.6 309,739 + PGA1_c03230 0.27 -1.5 309,749 + PGA1_c03230 0.29 -0.6 309,782 + PGA1_c03230 0.32 -0.5 309,827 - PGA1_c03230 0.37 -0.0 309,874 - PGA1_c03230 0.42 +0.2 309,927 + PGA1_c03230 0.48 -0.0 309,927 + PGA1_c03230 0.48 +0.2 309,929 + PGA1_c03230 0.49 -0.8 309,929 + PGA1_c03230 0.49 +1.0 309,937 - PGA1_c03230 0.49 -0.4 309,937 - PGA1_c03230 0.49 -2.2 309,937 - PGA1_c03230 0.49 +1.9 309,986 - PGA1_c03230 0.55 -1.8 309,990 + PGA1_c03230 0.55 +0.8 309,990 + PGA1_c03230 0.55 -2.0 309,998 - PGA1_c03230 0.56 +0.6 309,998 - PGA1_c03230 0.56 +1.8 310,026 + PGA1_c03230 0.59 -2.9 310,045 + PGA1_c03230 0.61 +1.1 310,069 + PGA1_c03230 0.64 +1.0 310,153 + PGA1_c03230 0.73 +0.2 310,158 + PGA1_c03230 0.74 -1.5 310,166 - PGA1_c03230 0.75 +0.6 310,299 - PGA1_c03230 0.89 -3.2 310,348 - -1.3 310,440 + +0.5 310,639 + PGA1_c03240 0.29 +0.6 310,675 - PGA1_c03240 0.34 -2.4 310,707 - PGA1_c03240 0.39 -1.8 310,721 - PGA1_c03240 0.41 +0.5 310,811 + PGA1_c03240 0.55 -1.5 310,816 + PGA1_c03240 0.56 -0.9 310,922 + PGA1_c03240 0.73 -1.1 310,971 - PGA1_c03240 0.80 +0.2 310,981 - PGA1_c03240 0.82 -0.8 310,990 + PGA1_c03240 0.83 -2.0 310,998 - PGA1_c03240 0.84 +0.5 311,024 - PGA1_c03240 0.88 -1.3 311,024 - PGA1_c03240 0.88 -1.3 311,049 + -1.6 311,057 - -0.6 311,076 + -0.1 311,099 + +2.3 311,099 + +2.4 311,107 - -2.0 311,107 - -0.5 311,107 - -0.2 311,107 - +1.3 311,107 - -1.5 311,109 + +0.7 311,124 - -1.2 311,179 + PGA1_c03250 0.17 +0.5 311,208 + PGA1_c03250 0.23 -3.0 311,221 - PGA1_c03250 0.26 +1.4 311,270 + PGA1_c03250 0.36 -0.8 311,331 + PGA1_c03250 0.48 +0.2 311,376 + PGA1_c03250 0.57 -0.8 311,411 - PGA1_c03250 0.64 +1.2 311,411 - PGA1_c03250 0.64 -2.1 311,420 + PGA1_c03250 0.66 +1.5 311,422 - PGA1_c03250 0.67 -0.4 311,452 + PGA1_c03250 0.73 +0.9 311,499 + PGA1_c03250 0.82 +0.7 311,508 + PGA1_c03250 0.84 +0.2 311,537 - +0.1 311,598 + +0.8 311,617 - -1.0 311,630 + +0.5 311,637 + +1.3 311,637 + +0.3 311,668 - PGA1_c03260 0.11 -1.2 311,707 - PGA1_c03260 0.15 -1.1 311,757 - PGA1_c03260 0.22 -1.5 311,841 + PGA1_c03260 0.32 -0.2 311,871 + PGA1_c03260 0.36 -1.0 311,871 + PGA1_c03260 0.36 +1.1 311,871 + PGA1_c03260 0.36 +1.4 311,913 + PGA1_c03260 0.41 -0.1 311,952 + PGA1_c03260 0.46 +0.4 311,962 + PGA1_c03260 0.47 -3.2 311,975 - PGA1_c03260 0.48 +0.2 311,984 - PGA1_c03260 0.49 +0.0 311,990 + PGA1_c03260 0.50 +0.7 311,998 - PGA1_c03260 0.51 -0.4 312,016 + PGA1_c03260 0.53 +0.8 312,048 + PGA1_c03260 0.57 +1.7 312,050 + PGA1_c03260 0.58 +0.0 312,050 + PGA1_c03260 0.58 -1.5 312,050 + PGA1_c03260 0.58 -0.8 312,050 + PGA1_c03260 0.58 +0.3 312,056 - PGA1_c03260 0.58 -1.8 312,058 - PGA1_c03260 0.59 +0.3
Or see this region's nucleotide sequence