Experiment: m.b. D-Cycloserine 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c02100 and PGA1_c02110 are separated by 66 nucleotides PGA1_c02110 and PGA1_c02120 are separated by 164 nucleotides PGA1_c02120 and PGA1_c02130 are separated by 5 nucleotides
PGA1_c02100: PGA1_c02100 - diguanylate cyclase (GGDEF) domain-containing protein, at 192,908 to 194,452
_c02100
PGA1_c02110: PGA1_c02110 - tRNA 2-thiocytidine biosynthesis protein TtcA, at 194,519 to 195,406
_c02110
PGA1_c02120: PGA1_c02120 - hypothetical protein, at 195,571 to 196,143
_c02120
PGA1_c02130: PGA1_c02130 - conserved hypothetical protein YidD, at 196,149 to 196,421
_c02130
Position (kb) 194
195
196 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 193.524 kb on + strand, within PGA1_c02100 at 193.591 kb on + strand, within PGA1_c02100 at 193.599 kb on - strand, within PGA1_c02100 at 193.649 kb on - strand, within PGA1_c02100 at 193.795 kb on + strand, within PGA1_c02100 at 193.800 kb on + strand, within PGA1_c02100 at 193.834 kb on + strand, within PGA1_c02100 at 193.834 kb on + strand, within PGA1_c02100 at 193.903 kb on - strand, within PGA1_c02100 at 193.930 kb on - strand, within PGA1_c02100 at 193.985 kb on - strand, within PGA1_c02100 at 194.019 kb on + strand, within PGA1_c02100 at 194.027 kb on - strand, within PGA1_c02100 at 194.072 kb on - strand, within PGA1_c02100 at 194.151 kb on - strand, within PGA1_c02100 at 194.174 kb on + strand, within PGA1_c02100 at 194.174 kb on + strand, within PGA1_c02100 at 194.174 kb on + strand, within PGA1_c02100 at 194.174 kb on + strand, within PGA1_c02100 at 194.182 kb on - strand, within PGA1_c02100 at 194.182 kb on - strand, within PGA1_c02100 at 194.182 kb on - strand, within PGA1_c02100 at 194.182 kb on - strand, within PGA1_c02100 at 194.182 kb on - strand, within PGA1_c02100 at 194.204 kb on - strand, within PGA1_c02100 at 194.240 kb on + strand, within PGA1_c02100 at 194.248 kb on - strand, within PGA1_c02100 at 194.248 kb on - strand, within PGA1_c02100 at 194.430 kb on - strand at 194.453 kb on + strand at 194.453 kb on + strand at 194.461 kb on - strand at 194.468 kb on - strand at 194.494 kb on - strand at 194.500 kb on - strand at 194.596 kb on + strand at 194.604 kb on - strand at 194.625 kb on + strand, within PGA1_c02110 at 194.633 kb on - strand, within PGA1_c02110 at 194.659 kb on + strand, within PGA1_c02110 at 194.669 kb on + strand, within PGA1_c02110 at 194.719 kb on + strand, within PGA1_c02110 at 194.946 kb on - strand, within PGA1_c02110 at 194.946 kb on - strand, within PGA1_c02110 at 194.946 kb on - strand, within PGA1_c02110 at 194.946 kb on - strand, within PGA1_c02110 at 194.975 kb on - strand, within PGA1_c02110 at 194.991 kb on + strand, within PGA1_c02110 at 194.992 kb on - strand, within PGA1_c02110 at 195.025 kb on - strand, within PGA1_c02110 at 195.128 kb on - strand, within PGA1_c02110 at 195.208 kb on + strand, within PGA1_c02110 at 195.208 kb on + strand, within PGA1_c02110 at 195.208 kb on + strand, within PGA1_c02110 at 195.208 kb on + strand, within PGA1_c02110 at 195.208 kb on + strand, within PGA1_c02110 at 195.208 kb on + strand, within PGA1_c02110 at 195.208 kb on + strand, within PGA1_c02110 at 195.208 kb on + strand, within PGA1_c02110 at 195.208 kb on + strand, within PGA1_c02110 at 195.208 kb on + strand, within PGA1_c02110 at 195.208 kb on + strand, within PGA1_c02110 at 195.208 kb on + strand, within PGA1_c02110 at 195.208 kb on + strand, within PGA1_c02110 at 195.208 kb on + strand, within PGA1_c02110 at 195.208 kb on + strand, within PGA1_c02110 at 195.208 kb on + strand, within PGA1_c02110 at 195.208 kb on + strand, within PGA1_c02110 at 195.208 kb on + strand, within PGA1_c02110 at 195.208 kb on + strand, within PGA1_c02110 at 195.208 kb on + strand, within PGA1_c02110 at 195.208 kb on + strand, within PGA1_c02110 at 195.210 kb on + strand, within PGA1_c02110 at 195.210 kb on + strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.218 kb on + strand, within PGA1_c02110 at 195.255 kb on - strand, within PGA1_c02110 at 195.272 kb on + strand, within PGA1_c02110 at 195.415 kb on - strand at 195.475 kb on - strand at 195.486 kb on - strand at 195.530 kb on + strand at 195.556 kb on + strand at 195.556 kb on + strand at 195.562 kb on + strand at 195.562 kb on + strand at 195.564 kb on - strand at 195.570 kb on - strand at 195.617 kb on + strand at 195.672 kb on + strand, within PGA1_c02120 at 195.768 kb on + strand, within PGA1_c02120 at 195.768 kb on + strand, within PGA1_c02120 at 195.776 kb on - strand, within PGA1_c02120 at 195.776 kb on - strand, within PGA1_c02120 at 195.776 kb on - strand, within PGA1_c02120 at 195.778 kb on + strand, within PGA1_c02120 at 195.778 kb on + strand, within PGA1_c02120 at 195.786 kb on - strand, within PGA1_c02120 at 195.786 kb on - strand, within PGA1_c02120 at 195.788 kb on + strand, within PGA1_c02120 at 195.796 kb on - strand, within PGA1_c02120 at 195.821 kb on + strand, within PGA1_c02120 at 195.821 kb on + strand, within PGA1_c02120 at 195.821 kb on + strand, within PGA1_c02120 at 195.829 kb on - strand, within PGA1_c02120 at 195.829 kb on - strand, within PGA1_c02120 at 195.829 kb on - strand, within PGA1_c02120 at 195.857 kb on - strand, within PGA1_c02120 at 195.866 kb on + strand, within PGA1_c02120 at 195.891 kb on + strand, within PGA1_c02120 at 195.942 kb on - strand, within PGA1_c02120 at 195.971 kb on - strand, within PGA1_c02120 at 195.979 kb on - strand, within PGA1_c02120 at 196.060 kb on + strand, within PGA1_c02120 at 196.084 kb on + strand, within PGA1_c02120 at 196.100 kb on + strand at 196.108 kb on - strand at 196.118 kb on - strand at 196.127 kb on + strand at 196.147 kb on + strand at 196.198 kb on - strand, within PGA1_c02130 at 196.211 kb on - strand, within PGA1_c02130 at 196.360 kb on + strand, within PGA1_c02130 at 196.373 kb on + strand, within PGA1_c02130
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. D-Cycloserine 0.0625 mg/ml remove 193,524 + PGA1_c02100 0.40 +0.3 193,591 + PGA1_c02100 0.44 -0.2 193,599 - PGA1_c02100 0.45 -1.5 193,649 - PGA1_c02100 0.48 -0.2 193,795 + PGA1_c02100 0.57 -1.5 193,800 + PGA1_c02100 0.58 +0.4 193,834 + PGA1_c02100 0.60 -0.0 193,834 + PGA1_c02100 0.60 +0.4 193,903 - PGA1_c02100 0.64 -1.7 193,930 - PGA1_c02100 0.66 -0.4 193,985 - PGA1_c02100 0.70 -2.5 194,019 + PGA1_c02100 0.72 -2.0 194,027 - PGA1_c02100 0.72 +0.1 194,072 - PGA1_c02100 0.75 -1.7 194,151 - PGA1_c02100 0.80 -1.5 194,174 + PGA1_c02100 0.82 -1.5 194,174 + PGA1_c02100 0.82 -1.0 194,174 + PGA1_c02100 0.82 -1.7 194,174 + PGA1_c02100 0.82 +0.3 194,182 - PGA1_c02100 0.82 -1.0 194,182 - PGA1_c02100 0.82 -0.4 194,182 - PGA1_c02100 0.82 +1.2 194,182 - PGA1_c02100 0.82 -2.6 194,182 - PGA1_c02100 0.82 +0.4 194,204 - PGA1_c02100 0.84 +1.3 194,240 + PGA1_c02100 0.86 -0.3 194,248 - PGA1_c02100 0.87 -1.5 194,248 - PGA1_c02100 0.87 -3.2 194,430 - -1.0 194,453 + -0.0 194,453 + +0.2 194,461 - +0.8 194,468 - +0.5 194,494 - -0.4 194,500 - +0.3 194,596 + +0.3 194,604 - +1.4 194,625 + PGA1_c02110 0.12 +0.2 194,633 - PGA1_c02110 0.13 +1.4 194,659 + PGA1_c02110 0.16 +0.5 194,669 + PGA1_c02110 0.17 +0.4 194,719 + PGA1_c02110 0.23 +0.8 194,946 - PGA1_c02110 0.48 +0.8 194,946 - PGA1_c02110 0.48 +1.7 194,946 - PGA1_c02110 0.48 +0.5 194,946 - PGA1_c02110 0.48 +0.1 194,975 - PGA1_c02110 0.51 -0.2 194,991 + PGA1_c02110 0.53 +1.2 194,992 - PGA1_c02110 0.53 +0.5 195,025 - PGA1_c02110 0.57 -1.7 195,128 - PGA1_c02110 0.69 -0.6 195,208 + PGA1_c02110 0.78 -0.7 195,208 + PGA1_c02110 0.78 -0.4 195,208 + PGA1_c02110 0.78 +0.4 195,208 + PGA1_c02110 0.78 +1.2 195,208 + PGA1_c02110 0.78 +2.0 195,208 + PGA1_c02110 0.78 +1.3 195,208 + PGA1_c02110 0.78 +1.0 195,208 + PGA1_c02110 0.78 -1.5 195,208 + PGA1_c02110 0.78 +1.3 195,208 + PGA1_c02110 0.78 -2.5 195,208 + PGA1_c02110 0.78 +0.3 195,208 + PGA1_c02110 0.78 -0.3 195,208 + PGA1_c02110 0.78 -1.9 195,208 + PGA1_c02110 0.78 +0.0 195,208 + PGA1_c02110 0.78 -0.7 195,208 + PGA1_c02110 0.78 -2.3 195,208 + PGA1_c02110 0.78 +1.4 195,208 + PGA1_c02110 0.78 -1.0 195,208 + PGA1_c02110 0.78 -1.9 195,208 + PGA1_c02110 0.78 +1.5 195,208 + PGA1_c02110 0.78 -0.7 195,210 + PGA1_c02110 0.78 +0.3 195,210 + PGA1_c02110 0.78 -0.3 195,216 - PGA1_c02110 0.78 +2.8 195,216 - PGA1_c02110 0.78 +1.2 195,216 - PGA1_c02110 0.78 +2.4 195,216 - PGA1_c02110 0.78 -0.5 195,216 - PGA1_c02110 0.78 -0.4 195,216 - PGA1_c02110 0.78 -0.9 195,216 - PGA1_c02110 0.78 +1.0 195,216 - PGA1_c02110 0.78 -0.3 195,216 - PGA1_c02110 0.78 +1.3 195,216 - PGA1_c02110 0.78 -1.5 195,216 - PGA1_c02110 0.78 -0.7 195,216 - PGA1_c02110 0.78 -1.6 195,216 - PGA1_c02110 0.78 -0.6 195,216 - PGA1_c02110 0.78 +0.5 195,216 - PGA1_c02110 0.78 -0.0 195,216 - PGA1_c02110 0.78 +0.8 195,216 - PGA1_c02110 0.78 +1.0 195,216 - PGA1_c02110 0.78 +0.8 195,216 - PGA1_c02110 0.78 -1.6 195,216 - PGA1_c02110 0.78 -1.7 195,216 - PGA1_c02110 0.78 -0.4 195,216 - PGA1_c02110 0.78 -0.7 195,216 - PGA1_c02110 0.78 +2.1 195,216 - PGA1_c02110 0.78 -1.0 195,216 - PGA1_c02110 0.78 -1.2 195,216 - PGA1_c02110 0.78 +1.6 195,216 - PGA1_c02110 0.78 +0.8 195,216 - PGA1_c02110 0.78 -0.3 195,216 - PGA1_c02110 0.78 +1.7 195,216 - PGA1_c02110 0.78 +1.6 195,218 + PGA1_c02110 0.79 -0.2 195,255 - PGA1_c02110 0.83 +0.3 195,272 + PGA1_c02110 0.85 +0.3 195,415 - +0.0 195,475 - -0.0 195,486 - -0.9 195,530 + -0.3 195,556 + +0.8 195,556 + -2.6 195,562 + -0.2 195,562 + +1.1 195,564 - +0.7 195,570 - +0.8 195,617 + -2.0 195,672 + PGA1_c02120 0.18 -0.9 195,768 + PGA1_c02120 0.34 +0.5 195,768 + PGA1_c02120 0.34 +0.6 195,776 - PGA1_c02120 0.36 +1.8 195,776 - PGA1_c02120 0.36 +0.3 195,776 - PGA1_c02120 0.36 -0.7 195,778 + PGA1_c02120 0.36 -1.4 195,778 + PGA1_c02120 0.36 -0.5 195,786 - PGA1_c02120 0.38 +1.7 195,786 - PGA1_c02120 0.38 -0.7 195,788 + PGA1_c02120 0.38 -1.2 195,796 - PGA1_c02120 0.39 +2.1 195,821 + PGA1_c02120 0.44 +0.6 195,821 + PGA1_c02120 0.44 -0.2 195,821 + PGA1_c02120 0.44 -2.5 195,829 - PGA1_c02120 0.45 +0.6 195,829 - PGA1_c02120 0.45 +0.3 195,829 - PGA1_c02120 0.45 -0.2 195,857 - PGA1_c02120 0.50 -2.3 195,866 + PGA1_c02120 0.51 +0.4 195,891 + PGA1_c02120 0.56 -0.3 195,942 - PGA1_c02120 0.65 -0.3 195,971 - PGA1_c02120 0.70 +0.8 195,979 - PGA1_c02120 0.71 -1.5 196,060 + PGA1_c02120 0.85 +2.2 196,084 + PGA1_c02120 0.90 +1.3 196,100 + +0.4 196,108 - -0.4 196,118 - +0.1 196,127 + +0.1 196,147 + +2.4 196,198 - PGA1_c02130 0.18 -0.2 196,211 - PGA1_c02130 0.23 +1.4 196,360 + PGA1_c02130 0.77 -0.9 196,373 + PGA1_c02130 0.82 +1.2
Or see this region's nucleotide sequence