Strain Fitness in Shewanella amazonensis SB2B around Sama_2316

Experiment: Tween 20

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntflgE and flgD are separated by 33 nucleotidesflgD and flgC are separated by 26 nucleotidesflgC and flgB are separated by 2 nucleotidesflgB and Sama_2319 are separated by 141 nucleotides Sama_2315: flgE - flagellar hook protein FlgE (RefSeq), at 2,780,894 to 2,782,255 flgE Sama_2316: flgD - flagellar basal body rod modification protein (RefSeq), at 2,782,289 to 2,783,017 flgD Sama_2317: flgC - flagellar basal body rod protein FlgC (RefSeq), at 2,783,044 to 2,783,457 flgC Sama_2318: flgB - flagellar basal body rod protein FlgB (RefSeq), at 2,783,460 to 2,783,858 flgB Sama_2319: Sama_2319 - protein-glutamate O-methyltransferase (RefSeq), at 2,784,000 to 2,784,833 _2319 Position (kb) 2782 2783 2784Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 2781.298 kb on - strand, within flgEat 2781.298 kb on - strand, within flgEat 2781.398 kb on + strand, within flgEat 2781.419 kb on - strand, within flgEat 2781.419 kb on - strand, within flgEat 2781.419 kb on - strand, within flgEat 2781.420 kb on + strand, within flgEat 2781.420 kb on + strand, within flgEat 2781.420 kb on + strand, within flgEat 2781.420 kb on + strand, within flgEat 2781.421 kb on - strand, within flgEat 2781.421 kb on - strand, within flgEat 2781.421 kb on - strand, within flgEat 2781.421 kb on - strand, within flgEat 2781.421 kb on - strand, within flgEat 2781.421 kb on - strand, within flgEat 2781.421 kb on - strand, within flgEat 2781.421 kb on - strand, within flgEat 2781.421 kb on - strand, within flgEat 2781.421 kb on - strand, within flgEat 2781.424 kb on + strand, within flgEat 2781.425 kb on - strand, within flgEat 2781.425 kb on - strand, within flgEat 2781.425 kb on - strand, within flgEat 2781.425 kb on - strand, within flgEat 2781.425 kb on - strand, within flgEat 2781.525 kb on + strand, within flgEat 2781.525 kb on + strand, within flgEat 2781.526 kb on - strand, within flgEat 2781.526 kb on - strand, within flgEat 2781.530 kb on - strand, within flgEat 2781.573 kb on + strand, within flgEat 2781.573 kb on + strand, within flgEat 2781.574 kb on - strand, within flgEat 2781.574 kb on - strand, within flgEat 2781.574 kb on - strand, within flgEat 2781.574 kb on - strand, within flgEat 2781.574 kb on - strand, within flgEat 2781.576 kb on + strand, within flgEat 2781.577 kb on - strand, within flgEat 2781.577 kb on - strand, within flgEat 2781.604 kb on - strand, within flgEat 2781.604 kb on - strand, within flgEat 2781.624 kb on - strand, within flgEat 2781.624 kb on - strand, within flgEat 2781.630 kb on + strand, within flgEat 2781.630 kb on + strand, within flgEat 2781.630 kb on + strand, within flgEat 2781.631 kb on - strand, within flgEat 2781.631 kb on - strand, within flgEat 2781.631 kb on - strand, within flgEat 2781.631 kb on - strand, within flgEat 2781.631 kb on - strand, within flgEat 2781.631 kb on - strand, within flgEat 2781.666 kb on + strand, within flgEat 2781.667 kb on - strand, within flgEat 2781.667 kb on - strand, within flgEat 2781.667 kb on - strand, within flgEat 2781.683 kb on - strand, within flgEat 2781.683 kb on - strand, within flgEat 2781.848 kb on - strand, within flgEat 2781.848 kb on - strand, within flgEat 2781.909 kb on + strand, within flgEat 2781.909 kb on + strand, within flgEat 2781.910 kb on - strand, within flgEat 2781.910 kb on - strand, within flgEat 2781.910 kb on - strand, within flgEat 2781.916 kb on + strand, within flgEat 2781.923 kb on + strand, within flgEat 2781.923 kb on + strand, within flgEat 2781.924 kb on - strand, within flgEat 2781.928 kb on + strand, within flgEat 2781.929 kb on - strand, within flgEat 2781.929 kb on - strand, within flgEat 2781.929 kb on - strand, within flgEat 2781.954 kb on + strand, within flgEat 2782.080 kb on - strand, within flgEat 2782.116 kb on - strand, within flgEat 2782.128 kb on + strandat 2782.128 kb on + strandat 2782.129 kb on - strandat 2782.129 kb on - strandat 2782.255 kb on + strandat 2782.256 kb on - strandat 2782.256 kb on - strandat 2782.256 kb on - strandat 2782.273 kb on - strandat 2782.401 kb on + strand, within flgDat 2782.402 kb on - strand, within flgDat 2782.402 kb on - strand, within flgDat 2782.402 kb on - strand, within flgDat 2782.402 kb on - strand, within flgDat 2782.416 kb on - strand, within flgDat 2782.461 kb on + strand, within flgDat 2782.461 kb on + strand, within flgDat 2782.461 kb on + strand, within flgDat 2782.462 kb on - strand, within flgDat 2782.462 kb on - strand, within flgDat 2782.462 kb on - strand, within flgDat 2782.462 kb on - strand, within flgDat 2782.462 kb on - strand, within flgDat 2782.462 kb on - strand, within flgDat 2782.462 kb on - strand, within flgDat 2782.465 kb on + strand, within flgDat 2782.466 kb on - strand, within flgDat 2782.466 kb on - strand, within flgDat 2782.653 kb on - strand, within flgDat 2782.653 kb on - strand, within flgDat 2782.653 kb on - strand, within flgDat 2782.653 kb on - strand, within flgDat 2782.653 kb on - strand, within flgDat 2782.653 kb on - strand, within flgDat 2782.653 kb on - strand, within flgDat 2782.653 kb on - strand, within flgDat 2782.653 kb on - strand, within flgDat 2782.680 kb on - strand, within flgDat 2782.680 kb on - strand, within flgDat 2782.680 kb on - strand, within flgDat 2782.736 kb on - strand, within flgDat 2782.736 kb on - strand, within flgDat 2783.000 kb on - strandat 2783.006 kb on - strandat 2783.022 kb on + strandat 2783.023 kb on - strandat 2783.077 kb on - strandat 2783.077 kb on - strandat 2783.077 kb on - strandat 2783.178 kb on + strand, within flgCat 2783.217 kb on - strand, within flgCat 2783.235 kb on + strand, within flgCat 2783.236 kb on - strand, within flgCat 2783.248 kb on + strand, within flgCat 2783.248 kb on + strand, within flgCat 2783.249 kb on - strand, within flgCat 2783.334 kb on - strand, within flgCat 2783.446 kb on - strandat 2783.472 kb on - strandat 2783.474 kb on + strandat 2783.487 kb on - strandat 2783.547 kb on - strand, within flgBat 2783.547 kb on - strand, within flgBat 2783.582 kb on + strand, within flgBat 2783.582 kb on + strand, within flgBat 2783.583 kb on - strand, within flgBat 2783.583 kb on - strand, within flgBat 2783.583 kb on - strand, within flgBat 2783.745 kb on - strand, within flgBat 2783.745 kb on - strand, within flgBat 2783.747 kb on - strand, within flgBat 2783.747 kb on - strand, within flgBat 2783.928 kb on - strandat 2783.928 kb on - strandat 2783.928 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Tween 20
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2,781,298 - flgE Sama_2315 0.30 -2.4
2,781,298 - flgE Sama_2315 0.30 -1.9
2,781,398 + flgE Sama_2315 0.37 -2.7
2,781,419 - flgE Sama_2315 0.39 -2.4
2,781,419 - flgE Sama_2315 0.39 -0.2
2,781,419 - flgE Sama_2315 0.39 -0.6
2,781,420 + flgE Sama_2315 0.39 -2.8
2,781,420 + flgE Sama_2315 0.39 -0.7
2,781,420 + flgE Sama_2315 0.39 -2.2
2,781,420 + flgE Sama_2315 0.39 -2.4
2,781,421 - flgE Sama_2315 0.39 -1.8
2,781,421 - flgE Sama_2315 0.39 -2.3
2,781,421 - flgE Sama_2315 0.39 -3.1
2,781,421 - flgE Sama_2315 0.39 -1.0
2,781,421 - flgE Sama_2315 0.39 -2.1
2,781,421 - flgE Sama_2315 0.39 -2.5
2,781,421 - flgE Sama_2315 0.39 -2.5
2,781,421 - flgE Sama_2315 0.39 -2.9
2,781,421 - flgE Sama_2315 0.39 -1.9
2,781,421 - flgE Sama_2315 0.39 -2.8
2,781,424 + flgE Sama_2315 0.39 +0.7
2,781,425 - flgE Sama_2315 0.39 -0.8
2,781,425 - flgE Sama_2315 0.39 -1.6
2,781,425 - flgE Sama_2315 0.39 -1.8
2,781,425 - flgE Sama_2315 0.39 -2.2
2,781,425 - flgE Sama_2315 0.39 -2.0
2,781,525 + flgE Sama_2315 0.46 -0.6
2,781,525 + flgE Sama_2315 0.46 -2.4
2,781,526 - flgE Sama_2315 0.46 -2.0
2,781,526 - flgE Sama_2315 0.46 -1.8
2,781,530 - flgE Sama_2315 0.47 -1.5
2,781,573 + flgE Sama_2315 0.50 -3.4
2,781,573 + flgE Sama_2315 0.50 -1.6
2,781,574 - flgE Sama_2315 0.50 -2.3
2,781,574 - flgE Sama_2315 0.50 -2.2
2,781,574 - flgE Sama_2315 0.50 -0.6
2,781,574 - flgE Sama_2315 0.50 -1.2
2,781,574 - flgE Sama_2315 0.50 -1.2
2,781,576 + flgE Sama_2315 0.50 -1.2
2,781,577 - flgE Sama_2315 0.50 -1.2
2,781,577 - flgE Sama_2315 0.50 -1.9
2,781,604 - flgE Sama_2315 0.52 -3.2
2,781,604 - flgE Sama_2315 0.52 -1.4
2,781,624 - flgE Sama_2315 0.54 -1.2
2,781,624 - flgE Sama_2315 0.54 -2.7
2,781,630 + flgE Sama_2315 0.54 -3.7
2,781,630 + flgE Sama_2315 0.54 -0.8
2,781,630 + flgE Sama_2315 0.54 -3.1
2,781,631 - flgE Sama_2315 0.54 -1.7
2,781,631 - flgE Sama_2315 0.54 -2.1
2,781,631 - flgE Sama_2315 0.54 -2.7
2,781,631 - flgE Sama_2315 0.54 -1.6
2,781,631 - flgE Sama_2315 0.54 -3.1
2,781,631 - flgE Sama_2315 0.54 -0.2
2,781,666 + flgE Sama_2315 0.57 -0.7
2,781,667 - flgE Sama_2315 0.57 -4.2
2,781,667 - flgE Sama_2315 0.57 -2.3
2,781,667 - flgE Sama_2315 0.57 -3.2
2,781,683 - flgE Sama_2315 0.58 -1.9
2,781,683 - flgE Sama_2315 0.58 -2.4
2,781,848 - flgE Sama_2315 0.70 -2.6
2,781,848 - flgE Sama_2315 0.70 -0.3
2,781,909 + flgE Sama_2315 0.75 -2.6
2,781,909 + flgE Sama_2315 0.75 -0.6
2,781,910 - flgE Sama_2315 0.75 -1.1
2,781,910 - flgE Sama_2315 0.75 -1.2
2,781,910 - flgE Sama_2315 0.75 -1.4
2,781,916 + flgE Sama_2315 0.75 -2.6
2,781,923 + flgE Sama_2315 0.76 -2.6
2,781,923 + flgE Sama_2315 0.76 -2.5
2,781,924 - flgE Sama_2315 0.76 -2.0
2,781,928 + flgE Sama_2315 0.76 -1.4
2,781,929 - flgE Sama_2315 0.76 -2.3
2,781,929 - flgE Sama_2315 0.76 -3.3
2,781,929 - flgE Sama_2315 0.76 -3.2
2,781,954 + flgE Sama_2315 0.78 -2.7
2,782,080 - flgE Sama_2315 0.87 -2.3
2,782,116 - flgE Sama_2315 0.90 -2.7
2,782,128 + -1.0
2,782,128 + -1.6
2,782,129 - -2.6
2,782,129 - -2.6
2,782,255 + -1.6
2,782,256 - -2.2
2,782,256 - -0.8
2,782,256 - -0.9
2,782,273 - -1.2
2,782,401 + flgD Sama_2316 0.15 +0.4
2,782,402 - flgD Sama_2316 0.16 -3.4
2,782,402 - flgD Sama_2316 0.16 -2.3
2,782,402 - flgD Sama_2316 0.16 -1.8
2,782,402 - flgD Sama_2316 0.16 +1.4
2,782,416 - flgD Sama_2316 0.17 -2.8
2,782,461 + flgD Sama_2316 0.24 -0.0
2,782,461 + flgD Sama_2316 0.24 -2.2
2,782,461 + flgD Sama_2316 0.24 -2.1
2,782,462 - flgD Sama_2316 0.24 -1.7
2,782,462 - flgD Sama_2316 0.24 -1.2
2,782,462 - flgD Sama_2316 0.24 -1.6
2,782,462 - flgD Sama_2316 0.24 -3.5
2,782,462 - flgD Sama_2316 0.24 -3.3
2,782,462 - flgD Sama_2316 0.24 -1.5
2,782,462 - flgD Sama_2316 0.24 -2.0
2,782,465 + flgD Sama_2316 0.24 -1.2
2,782,466 - flgD Sama_2316 0.24 -2.9
2,782,466 - flgD Sama_2316 0.24 -2.8
2,782,653 - flgD Sama_2316 0.50 -2.6
2,782,653 - flgD Sama_2316 0.50 -3.3
2,782,653 - flgD Sama_2316 0.50 -2.8
2,782,653 - flgD Sama_2316 0.50 -1.8
2,782,653 - flgD Sama_2316 0.50 -3.2
2,782,653 - flgD Sama_2316 0.50 -3.3
2,782,653 - flgD Sama_2316 0.50 -1.5
2,782,653 - flgD Sama_2316 0.50 -1.8
2,782,653 - flgD Sama_2316 0.50 -2.4
2,782,680 - flgD Sama_2316 0.54 -2.4
2,782,680 - flgD Sama_2316 0.54 -2.2
2,782,680 - flgD Sama_2316 0.54 -2.6
2,782,736 - flgD Sama_2316 0.61 -1.6
2,782,736 - flgD Sama_2316 0.61 -1.9
2,783,000 - -0.2
2,783,006 - -1.2
2,783,022 + -2.1
2,783,023 - -1.2
2,783,077 - -2.8
2,783,077 - -1.9
2,783,077 - -0.9
2,783,178 + flgC Sama_2317 0.32 -1.9
2,783,217 - flgC Sama_2317 0.42 -1.8
2,783,235 + flgC Sama_2317 0.46 -2.7
2,783,236 - flgC Sama_2317 0.46 -1.9
2,783,248 + flgC Sama_2317 0.49 -2.7
2,783,248 + flgC Sama_2317 0.49 +0.4
2,783,249 - flgC Sama_2317 0.50 -2.9
2,783,334 - flgC Sama_2317 0.70 -2.4
2,783,446 - -1.2
2,783,472 - -1.9
2,783,474 + -1.2
2,783,487 - -1.4
2,783,547 - flgB Sama_2318 0.22 -1.9
2,783,547 - flgB Sama_2318 0.22 +0.1
2,783,582 + flgB Sama_2318 0.31 -0.6
2,783,582 + flgB Sama_2318 0.31 -0.8
2,783,583 - flgB Sama_2318 0.31 -3.2
2,783,583 - flgB Sama_2318 0.31 -2.4
2,783,583 - flgB Sama_2318 0.31 -1.2
2,783,745 - flgB Sama_2318 0.71 -2.3
2,783,745 - flgB Sama_2318 0.71 -0.9
2,783,747 - flgB Sama_2318 0.72 -0.6
2,783,747 - flgB Sama_2318 0.72 -1.8
2,783,928 - +0.3
2,783,928 - +0.7
2,783,928 - +1.0

Or see this region's nucleotide sequence