Experiment: Tween 20
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_2146 and Sama_2147 are separated by 44 nucleotides Sama_2147 and Sama_2148 are separated by 104 nucleotides Sama_2148 and Sama_2149 are separated by 12 nucleotides
Sama_2146: Sama_2146 - amidophosphoribosyltransferase (RefSeq), at 2,592,502 to 2,594,016
_2146
Sama_2147: Sama_2147 - colicin V production protein (RefSeq), at 2,594,061 to 2,594,549
_2147
Sama_2148: Sama_2148 - sporulation related (RefSeq), at 2,594,654 to 2,595,325
_2148
Sama_2149: Sama_2149 - FolC bifunctional protein (RefSeq), at 2,595,338 to 2,596,603
_2149
Position (kb)
2594
2595
2596 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2593.964 kb on + strand at 2593.964 kb on + strand at 2593.965 kb on - strand at 2593.965 kb on - strand at 2593.965 kb on - strand at 2593.965 kb on - strand at 2593.965 kb on - strand at 2593.965 kb on - strand at 2593.965 kb on - strand at 2593.965 kb on - strand at 2593.965 kb on - strand at 2593.996 kb on + strand at 2594.008 kb on - strand at 2594.035 kb on - strand at 2594.064 kb on + strand at 2594.290 kb on - strand, within Sama_2147 at 2594.306 kb on - strand, within Sama_2147 at 2594.338 kb on - strand, within Sama_2147 at 2594.349 kb on - strand, within Sama_2147 at 2594.349 kb on - strand, within Sama_2147 at 2594.417 kb on - strand, within Sama_2147 at 2594.499 kb on - strand, within Sama_2147 at 2594.534 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Tween 20 remove 2,593,964 + -4.4 2,593,964 + -2.9 2,593,965 - -3.5 2,593,965 - -3.3 2,593,965 - -2.4 2,593,965 - -3.1 2,593,965 - -1.7 2,593,965 - -1.3 2,593,965 - -1.9 2,593,965 - -1.9 2,593,965 - -1.2 2,593,996 + -2.3 2,594,008 - -1.3 2,594,035 - +0.5 2,594,064 + -0.5 2,594,290 - Sama_2147 0.47 -0.0 2,594,306 - Sama_2147 0.50 +0.5 2,594,338 - Sama_2147 0.57 -0.6 2,594,349 - Sama_2147 0.59 -0.5 2,594,349 - Sama_2147 0.59 +0.8 2,594,417 - Sama_2147 0.73 -0.2 2,594,499 - Sama_2147 0.90 -0.6 2,594,534 - -1.5
Or see this region's nucleotide sequence