Experiment: Tween 20
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_1647 and Sama_1648 overlap by 4 nucleotides Sama_1648 and Sama_1649 are separated by 89 nucleotides Sama_1649 and Sama_1650 are separated by 71 nucleotides
Sama_1647: Sama_1647 - succinyl-diaminopimelate desuccinylase (RefSeq), at 2,012,345 to 2,013,493
_1647
Sama_1648: Sama_1648 - hypothetical protein (RefSeq), at 2,013,490 to 2,013,834
_1648
Sama_1649: Sama_1649 - glucose-specific PTS system component (RefSeq), at 2,013,924 to 2,014,433
_1649
Sama_1650: Sama_1650 - phosphotransferase system, enzyme I (RefSeq), at 2,014,505 to 2,016,208
_1650
Position (kb)
2013
2014 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2013.513 kb on - strand at 2013.513 kb on - strand at 2013.537 kb on - strand, within Sama_1648 at 2013.620 kb on - strand, within Sama_1648 at 2013.886 kb on - strand at 2013.886 kb on - strand at 2013.939 kb on - strand at 2013.988 kb on + strand, within Sama_1649 at 2014.196 kb on + strand, within Sama_1649 at 2014.230 kb on + strand, within Sama_1649 at 2014.231 kb on - strand, within Sama_1649 at 2014.454 kb on - strand at 2014.490 kb on - strand at 2014.507 kb on - strand at 2014.510 kb on + strand at 2014.510 kb on + strand at 2014.511 kb on - strand at 2014.511 kb on - strand at 2014.512 kb on + strand at 2014.513 kb on - strand at 2014.516 kb on + strand at 2014.517 kb on - strand at 2014.521 kb on - strand at 2014.521 kb on - strand at 2014.521 kb on - strand at 2014.523 kb on - strand at 2014.523 kb on - strand at 2014.523 kb on - strand at 2014.523 kb on - strand at 2014.670 kb on + strand at 2014.670 kb on + strand at 2014.671 kb on - strand at 2014.719 kb on - strand, within Sama_1650 at 2014.763 kb on + strand, within Sama_1650
Per-strain Table
Position Strand Gene LocusTag Fraction Tween 20 remove 2,013,513 - +0.1 2,013,513 - +0.6 2,013,537 - Sama_1648 0.14 -0.2 2,013,620 - Sama_1648 0.38 -1.1 2,013,886 - -0.6 2,013,886 - +0.4 2,013,939 - -0.6 2,013,988 + Sama_1649 0.13 +1.1 2,014,196 + Sama_1649 0.53 +1.5 2,014,230 + Sama_1649 0.60 +1.1 2,014,231 - Sama_1649 0.60 +0.5 2,014,454 - -0.0 2,014,490 - -0.8 2,014,507 - -0.9 2,014,510 + +0.4 2,014,510 + +1.3 2,014,511 - -0.2 2,014,511 - -1.3 2,014,512 + +1.1 2,014,513 - -0.2 2,014,516 + +0.8 2,014,517 - -0.3 2,014,521 - -0.4 2,014,521 - -0.3 2,014,521 - -0.6 2,014,523 - -2.5 2,014,523 - -1.3 2,014,523 - -2.9 2,014,523 - -0.3 2,014,670 + -2.5 2,014,670 + -1.3 2,014,671 - -1.9 2,014,719 - Sama_1650 0.13 -1.4 2,014,763 + Sama_1650 0.15 -1.9
Or see this region's nucleotide sequence