Experiment: m.b. A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c00990 and PGA1_c01000 are separated by 92 nucleotides PGA1_c01000 and PGA1_c01010 are separated by 40 nucleotides PGA1_c01010 and PGA1_c01020 are separated by 33 nucleotides PGA1_c01020 and PGA1_c01030 are separated by 99 nucleotides
PGA1_c00990: PGA1_c00990 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase FolK, at 93,706 to 94,275
_c00990
PGA1_c01000: PGA1_c01000 - DNA-directed RNA polymerase subunit omega, at 94,368 to 94,721
_c01000
PGA1_c01010: PGA1_c01010 - guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase SpoT, at 94,762 to 96,894
_c01010
PGA1_c01020: PGA1_c01020 - Uncharacterized protein conserved in bacteria, at 96,928 to 97,581
_c01020
PGA1_c01030: PGA1_c01030 - pyridoxine 5'-phosphate synthase PdxJ, at 97,681 to 98,442
_c01030
Position (kb)
94
95
96
97 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 94.269 kb on + strand at 94.269 kb on + strand at 94.269 kb on + strand at 94.277 kb on - strand at 94.277 kb on - strand at 94.300 kb on - strand at 94.632 kb on - strand, within PGA1_c01000 at 94.646 kb on + strand, within PGA1_c01000 at 94.718 kb on - strand at 94.723 kb on - strand at 94.786 kb on + strand at 94.794 kb on - strand at 94.794 kb on - strand at 94.815 kb on - strand at 94.815 kb on - strand at 94.889 kb on + strand at 94.893 kb on - strand at 94.903 kb on - strand at 94.940 kb on + strand at 94.964 kb on + strand at 95.023 kb on + strand, within PGA1_c01010 at 95.033 kb on - strand, within PGA1_c01010 at 95.033 kb on - strand, within PGA1_c01010 at 95.056 kb on + strand, within PGA1_c01010 at 95.127 kb on + strand, within PGA1_c01010 at 95.127 kb on + strand, within PGA1_c01010 at 95.135 kb on - strand, within PGA1_c01010 at 95.135 kb on - strand, within PGA1_c01010 at 95.147 kb on - strand, within PGA1_c01010 at 95.171 kb on - strand, within PGA1_c01010 at 95.178 kb on - strand, within PGA1_c01010 at 95.280 kb on - strand, within PGA1_c01010 at 95.328 kb on + strand, within PGA1_c01010 at 95.333 kb on + strand, within PGA1_c01010 at 95.333 kb on + strand, within PGA1_c01010 at 95.336 kb on - strand, within PGA1_c01010 at 95.341 kb on - strand, within PGA1_c01010 at 95.439 kb on - strand, within PGA1_c01010 at 95.520 kb on - strand, within PGA1_c01010 at 95.520 kb on - strand, within PGA1_c01010 at 95.579 kb on - strand, within PGA1_c01010 at 95.583 kb on - strand, within PGA1_c01010 at 95.612 kb on + strand, within PGA1_c01010 at 95.620 kb on - strand, within PGA1_c01010 at 95.634 kb on - strand, within PGA1_c01010 at 95.642 kb on + strand, within PGA1_c01010 at 95.650 kb on - strand, within PGA1_c01010 at 95.719 kb on - strand, within PGA1_c01010 at 95.727 kb on - strand, within PGA1_c01010 at 95.734 kb on + strand, within PGA1_c01010 at 95.734 kb on + strand, within PGA1_c01010 at 95.747 kb on + strand, within PGA1_c01010 at 95.772 kb on + strand, within PGA1_c01010 at 95.811 kb on - strand, within PGA1_c01010 at 95.854 kb on - strand, within PGA1_c01010 at 95.856 kb on + strand, within PGA1_c01010 at 95.856 kb on + strand, within PGA1_c01010 at 95.864 kb on - strand, within PGA1_c01010 at 95.907 kb on + strand, within PGA1_c01010 at 95.907 kb on + strand, within PGA1_c01010 at 95.915 kb on - strand, within PGA1_c01010 at 95.940 kb on + strand, within PGA1_c01010 at 95.950 kb on - strand, within PGA1_c01010 at 95.951 kb on - strand, within PGA1_c01010 at 95.961 kb on + strand, within PGA1_c01010 at 95.961 kb on + strand, within PGA1_c01010 at 95.961 kb on + strand, within PGA1_c01010 at 95.961 kb on + strand, within PGA1_c01010 at 95.969 kb on - strand, within PGA1_c01010 at 95.969 kb on - strand, within PGA1_c01010 at 95.969 kb on - strand, within PGA1_c01010 at 95.969 kb on - strand, within PGA1_c01010 at 95.969 kb on - strand, within PGA1_c01010 at 95.977 kb on - strand, within PGA1_c01010 at 95.991 kb on - strand, within PGA1_c01010 at 95.998 kb on + strand, within PGA1_c01010 at 95.998 kb on + strand, within PGA1_c01010 at 96.021 kb on - strand, within PGA1_c01010 at 96.031 kb on - strand, within PGA1_c01010 at 96.067 kb on + strand, within PGA1_c01010 at 96.075 kb on - strand, within PGA1_c01010 at 96.075 kb on - strand, within PGA1_c01010 at 96.131 kb on + strand, within PGA1_c01010 at 96.159 kb on - strand, within PGA1_c01010 at 96.262 kb on + strand, within PGA1_c01010 at 96.296 kb on + strand, within PGA1_c01010 at 96.298 kb on + strand, within PGA1_c01010 at 96.298 kb on + strand, within PGA1_c01010 at 96.298 kb on + strand, within PGA1_c01010 at 96.304 kb on - strand, within PGA1_c01010 at 96.304 kb on - strand, within PGA1_c01010 at 96.306 kb on - strand, within PGA1_c01010 at 96.380 kb on + strand, within PGA1_c01010 at 96.388 kb on - strand, within PGA1_c01010 at 96.388 kb on - strand, within PGA1_c01010 at 96.388 kb on - strand, within PGA1_c01010 at 96.412 kb on + strand, within PGA1_c01010 at 96.412 kb on - strand, within PGA1_c01010 at 96.430 kb on + strand, within PGA1_c01010 at 96.448 kb on + strand, within PGA1_c01010 at 96.456 kb on - strand, within PGA1_c01010 at 96.456 kb on - strand, within PGA1_c01010 at 96.515 kb on + strand, within PGA1_c01010 at 96.535 kb on - strand, within PGA1_c01010 at 96.535 kb on - strand, within PGA1_c01010 at 96.567 kb on + strand, within PGA1_c01010 at 96.567 kb on + strand, within PGA1_c01010 at 96.600 kb on + strand, within PGA1_c01010 at 96.618 kb on - strand, within PGA1_c01010 at 96.628 kb on + strand, within PGA1_c01010 at 96.635 kb on + strand, within PGA1_c01010 at 96.643 kb on - strand, within PGA1_c01010 at 96.673 kb on + strand, within PGA1_c01010 at 96.673 kb on + strand, within PGA1_c01010 at 96.673 kb on + strand, within PGA1_c01010 at 96.673 kb on + strand, within PGA1_c01010 at 96.673 kb on + strand, within PGA1_c01010 at 96.681 kb on - strand at 96.681 kb on - strand at 96.681 kb on - strand at 96.684 kb on + strand at 96.684 kb on + strand at 96.692 kb on - strand at 96.727 kb on - strand at 96.858 kb on - strand at 96.858 kb on - strand at 96.858 kb on - strand at 97.487 kb on + strand, within PGA1_c01020 at 97.559 kb on + strand at 97.575 kb on + strand at 97.621 kb on + strand at 97.621 kb on + strand at 97.629 kb on - strand at 97.631 kb on + strand at 97.642 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. A22 0.02 mg/ml remove 94,269 + +0.7 94,269 + +3.9 94,269 + +0.8 94,277 - -0.2 94,277 - -0.2 94,300 - -1.1 94,632 - PGA1_c01000 0.75 -1.0 94,646 + PGA1_c01000 0.79 -0.7 94,718 - -2.2 94,723 - -2.6 94,786 + -0.4 94,794 - -0.5 94,794 - -0.5 94,815 - -0.3 94,815 - +0.7 94,889 + -2.3 94,893 - -2.0 94,903 - +0.0 94,940 + +0.1 94,964 + -1.1 95,023 + PGA1_c01010 0.12 -1.0 95,033 - PGA1_c01010 0.13 -0.9 95,033 - PGA1_c01010 0.13 -1.4 95,056 + PGA1_c01010 0.14 -2.1 95,127 + PGA1_c01010 0.17 -1.2 95,127 + PGA1_c01010 0.17 +0.3 95,135 - PGA1_c01010 0.17 -0.1 95,135 - PGA1_c01010 0.17 -1.9 95,147 - PGA1_c01010 0.18 -0.7 95,171 - PGA1_c01010 0.19 -0.3 95,178 - PGA1_c01010 0.20 -0.7 95,280 - PGA1_c01010 0.24 -0.5 95,328 + PGA1_c01010 0.27 -1.2 95,333 + PGA1_c01010 0.27 -0.9 95,333 + PGA1_c01010 0.27 -0.3 95,336 - PGA1_c01010 0.27 +0.2 95,341 - PGA1_c01010 0.27 -1.3 95,439 - PGA1_c01010 0.32 -1.3 95,520 - PGA1_c01010 0.36 -0.5 95,520 - PGA1_c01010 0.36 -3.3 95,579 - PGA1_c01010 0.38 +0.5 95,583 - PGA1_c01010 0.38 -0.9 95,612 + PGA1_c01010 0.40 -1.9 95,620 - PGA1_c01010 0.40 -1.2 95,634 - PGA1_c01010 0.41 -0.1 95,642 + PGA1_c01010 0.41 +1.9 95,650 - PGA1_c01010 0.42 -0.3 95,719 - PGA1_c01010 0.45 -2.1 95,727 - PGA1_c01010 0.45 -0.4 95,734 + PGA1_c01010 0.46 +0.2 95,734 + PGA1_c01010 0.46 -1.4 95,747 + PGA1_c01010 0.46 -1.7 95,772 + PGA1_c01010 0.47 -2.7 95,811 - PGA1_c01010 0.49 +0.2 95,854 - PGA1_c01010 0.51 -0.3 95,856 + PGA1_c01010 0.51 -0.9 95,856 + PGA1_c01010 0.51 -1.2 95,864 - PGA1_c01010 0.52 +1.0 95,907 + PGA1_c01010 0.54 -0.7 95,907 + PGA1_c01010 0.54 -0.2 95,915 - PGA1_c01010 0.54 +0.1 95,940 + PGA1_c01010 0.55 -0.5 95,950 - PGA1_c01010 0.56 -0.3 95,951 - PGA1_c01010 0.56 -1.2 95,961 + PGA1_c01010 0.56 -0.4 95,961 + PGA1_c01010 0.56 -0.3 95,961 + PGA1_c01010 0.56 -0.6 95,961 + PGA1_c01010 0.56 -0.6 95,969 - PGA1_c01010 0.57 -1.3 95,969 - PGA1_c01010 0.57 -2.2 95,969 - PGA1_c01010 0.57 -0.6 95,969 - PGA1_c01010 0.57 -0.2 95,969 - PGA1_c01010 0.57 +2.1 95,977 - PGA1_c01010 0.57 -1.7 95,991 - PGA1_c01010 0.58 -0.0 95,998 + PGA1_c01010 0.58 +0.6 95,998 + PGA1_c01010 0.58 +0.0 96,021 - PGA1_c01010 0.59 -1.0 96,031 - PGA1_c01010 0.59 -1.9 96,067 + PGA1_c01010 0.61 +0.2 96,075 - PGA1_c01010 0.62 -2.6 96,075 - PGA1_c01010 0.62 -0.9 96,131 + PGA1_c01010 0.64 -1.0 96,159 - PGA1_c01010 0.65 -1.6 96,262 + PGA1_c01010 0.70 -0.2 96,296 + PGA1_c01010 0.72 -1.3 96,298 + PGA1_c01010 0.72 -0.5 96,298 + PGA1_c01010 0.72 -1.2 96,298 + PGA1_c01010 0.72 +0.7 96,304 - PGA1_c01010 0.72 -0.5 96,304 - PGA1_c01010 0.72 -0.0 96,306 - PGA1_c01010 0.72 -0.6 96,380 + PGA1_c01010 0.76 -0.6 96,388 - PGA1_c01010 0.76 -0.4 96,388 - PGA1_c01010 0.76 -0.1 96,388 - PGA1_c01010 0.76 +0.6 96,412 + PGA1_c01010 0.77 -0.8 96,412 - PGA1_c01010 0.77 -0.0 96,430 + PGA1_c01010 0.78 -2.6 96,448 + PGA1_c01010 0.79 -1.2 96,456 - PGA1_c01010 0.79 +0.2 96,456 - PGA1_c01010 0.79 +0.8 96,515 + PGA1_c01010 0.82 +0.5 96,535 - PGA1_c01010 0.83 -1.1 96,535 - PGA1_c01010 0.83 +0.5 96,567 + PGA1_c01010 0.85 +0.3 96,567 + PGA1_c01010 0.85 -0.9 96,600 + PGA1_c01010 0.86 -0.7 96,618 - PGA1_c01010 0.87 +0.7 96,628 + PGA1_c01010 0.87 +0.2 96,635 + PGA1_c01010 0.88 +0.3 96,643 - PGA1_c01010 0.88 -0.4 96,673 + PGA1_c01010 0.90 -0.7 96,673 + PGA1_c01010 0.90 +0.1 96,673 + PGA1_c01010 0.90 -1.6 96,673 + PGA1_c01010 0.90 -1.3 96,673 + PGA1_c01010 0.90 -0.5 96,681 - -0.4 96,681 - -0.0 96,681 - -1.3 96,684 + +1.3 96,684 + -1.1 96,692 - +0.0 96,727 - -0.8 96,858 - +1.7 96,858 - +0.1 96,858 - -1.2 97,487 + PGA1_c01020 0.85 +0.1 97,559 + -0.3 97,575 + +0.7 97,621 + -0.3 97,621 + +0.1 97,629 - -0.6 97,631 + -1.9 97,642 + -1.1
Or see this region's nucleotide sequence