Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0929 and Psest_0930 are separated by 28 nucleotides Psest_0930 and Psest_0931 are separated by 233 nucleotides Psest_0931 and Psest_0932 are separated by 45 nucleotides
Psest_0929: Psest_0929 - ABC-type proline/glycine betaine transport systems, periplasmic components, at 1,007,380 to 1,008,159
_0929
Psest_0930: Psest_0930 - Nitroreductase, at 1,008,188 to 1,008,841
_0930
Psest_0931: Psest_0931 - putative quinone oxidoreductase, YhdH/YhfP family, at 1,009,075 to 1,010,058
_0931
Psest_0932: Psest_0932 - Transcriptional regulator, at 1,010,104 to 1,010,736
_0932
Position (kb)
1009
1010
1011 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1008.167 kb on - strand at 1008.167 kb on - strand at 1008.219 kb on - strand at 1008.219 kb on - strand at 1008.343 kb on + strand, within Psest_0930 at 1008.343 kb on + strand, within Psest_0930 at 1008.344 kb on - strand, within Psest_0930 at 1008.344 kb on - strand, within Psest_0930 at 1008.385 kb on + strand, within Psest_0930 at 1008.385 kb on + strand, within Psest_0930 at 1008.386 kb on - strand, within Psest_0930 at 1008.395 kb on + strand, within Psest_0930 at 1008.395 kb on + strand, within Psest_0930 at 1008.395 kb on + strand, within Psest_0930 at 1008.395 kb on + strand, within Psest_0930 at 1008.395 kb on + strand, within Psest_0930 at 1008.396 kb on - strand, within Psest_0930 at 1008.396 kb on - strand, within Psest_0930 at 1008.396 kb on - strand, within Psest_0930 at 1008.396 kb on - strand, within Psest_0930 at 1008.483 kb on + strand, within Psest_0930 at 1008.483 kb on + strand, within Psest_0930 at 1008.483 kb on + strand, within Psest_0930 at 1008.484 kb on - strand, within Psest_0930 at 1008.484 kb on - strand, within Psest_0930 at 1008.484 kb on - strand, within Psest_0930 at 1008.557 kb on + strand, within Psest_0930 at 1008.561 kb on + strand, within Psest_0930 at 1008.561 kb on + strand, within Psest_0930 at 1008.561 kb on + strand, within Psest_0930 at 1008.562 kb on - strand, within Psest_0930 at 1008.562 kb on - strand, within Psest_0930 at 1008.562 kb on - strand, within Psest_0930 at 1008.562 kb on - strand, within Psest_0930 at 1008.617 kb on + strand, within Psest_0930 at 1008.617 kb on + strand, within Psest_0930 at 1008.617 kb on + strand, within Psest_0930 at 1008.617 kb on + strand, within Psest_0930 at 1008.617 kb on + strand, within Psest_0930 at 1008.617 kb on + strand, within Psest_0930 at 1008.617 kb on + strand, within Psest_0930 at 1008.617 kb on + strand, within Psest_0930 at 1008.617 kb on + strand, within Psest_0930 at 1008.618 kb on - strand, within Psest_0930 at 1008.618 kb on - strand, within Psest_0930 at 1008.618 kb on - strand, within Psest_0930 at 1008.618 kb on - strand, within Psest_0930 at 1008.618 kb on - strand, within Psest_0930 at 1008.618 kb on - strand, within Psest_0930 at 1008.618 kb on - strand, within Psest_0930 at 1008.618 kb on - strand, within Psest_0930 at 1008.618 kb on - strand, within Psest_0930 at 1008.736 kb on + strand, within Psest_0930 at 1008.835 kb on + strand at 1008.835 kb on + strand at 1008.835 kb on + strand at 1008.835 kb on + strand at 1008.835 kb on + strand at 1008.836 kb on - strand at 1008.836 kb on - strand at 1008.839 kb on + strand at 1008.857 kb on - strand at 1008.942 kb on + strand at 1008.994 kb on - strand at 1009.057 kb on + strand at 1009.059 kb on + strand at 1009.060 kb on - strand at 1009.090 kb on + strand at 1009.090 kb on + strand at 1009.090 kb on + strand at 1009.091 kb on - strand at 1009.091 kb on - strand at 1009.119 kb on + strand at 1009.119 kb on + strand at 1009.119 kb on + strand at 1009.120 kb on - strand at 1009.120 kb on - strand at 1009.120 kb on - strand at 1009.120 kb on - strand at 1009.120 kb on - strand at 1009.120 kb on - strand at 1009.123 kb on + strand at 1009.123 kb on + strand at 1009.124 kb on - strand at 1009.124 kb on - strand at 1009.183 kb on + strand, within Psest_0931 at 1009.238 kb on + strand, within Psest_0931 at 1009.238 kb on + strand, within Psest_0931 at 1009.238 kb on + strand, within Psest_0931 at 1009.238 kb on + strand, within Psest_0931 at 1009.239 kb on - strand, within Psest_0931 at 1009.239 kb on - strand, within Psest_0931 at 1009.239 kb on - strand, within Psest_0931 at 1009.239 kb on - strand, within Psest_0931 at 1009.273 kb on + strand, within Psest_0931 at 1009.273 kb on + strand, within Psest_0931 at 1009.273 kb on + strand, within Psest_0931 at 1009.273 kb on + strand, within Psest_0931 at 1009.273 kb on + strand, within Psest_0931 at 1009.273 kb on + strand, within Psest_0931 at 1009.274 kb on - strand, within Psest_0931 at 1009.274 kb on - strand, within Psest_0931 at 1009.274 kb on - strand, within Psest_0931 at 1009.274 kb on - strand, within Psest_0931 at 1009.274 kb on - strand, within Psest_0931 at 1009.274 kb on - strand, within Psest_0931 at 1009.274 kb on - strand, within Psest_0931 at 1009.327 kb on + strand, within Psest_0931 at 1009.327 kb on + strand, within Psest_0931 at 1009.372 kb on + strand, within Psest_0931 at 1009.372 kb on + strand, within Psest_0931 at 1009.372 kb on + strand, within Psest_0931 at 1009.372 kb on + strand, within Psest_0931 at 1009.372 kb on + strand, within Psest_0931 at 1009.373 kb on - strand, within Psest_0931 at 1009.373 kb on - strand, within Psest_0931 at 1009.378 kb on - strand, within Psest_0931 at 1009.387 kb on - strand, within Psest_0931 at 1009.387 kb on - strand, within Psest_0931 at 1009.387 kb on - strand, within Psest_0931 at 1009.476 kb on + strand, within Psest_0931 at 1009.499 kb on + strand, within Psest_0931 at 1009.500 kb on - strand, within Psest_0931 at 1009.606 kb on + strand, within Psest_0931 at 1009.669 kb on - strand, within Psest_0931 at 1009.669 kb on - strand, within Psest_0931 at 1009.669 kb on - strand, within Psest_0931 at 1009.685 kb on - strand, within Psest_0931 at 1009.787 kb on - strand, within Psest_0931 at 1009.802 kb on - strand, within Psest_0931 at 1009.826 kb on + strand, within Psest_0931 at 1009.827 kb on - strand, within Psest_0931 at 1009.918 kb on - strand, within Psest_0931 at 1009.934 kb on + strand, within Psest_0931 at 1009.935 kb on - strand, within Psest_0931 at 1009.935 kb on - strand, within Psest_0931 at 1009.935 kb on - strand, within Psest_0931 at 1009.935 kb on - strand, within Psest_0931 at 1009.935 kb on - strand, within Psest_0931 at 1009.935 kb on - strand, within Psest_0931 at 1009.949 kb on + strand, within Psest_0931 at 1009.949 kb on + strand, within Psest_0931 at 1009.950 kb on - strand, within Psest_0931 at 1009.950 kb on - strand, within Psest_0931 at 1009.950 kb on - strand, within Psest_0931 at 1010.006 kb on - strand at 1010.029 kb on - strand at 1010.029 kb on - strand at 1010.029 kb on - strand at 1010.040 kb on + strand at 1010.245 kb on + strand, within Psest_0932 at 1010.246 kb on - strand, within Psest_0932 at 1010.246 kb on - strand, within Psest_0932 at 1010.246 kb on - strand, within Psest_0932 at 1010.246 kb on - strand, within Psest_0932 at 1010.310 kb on + strand, within Psest_0932 at 1010.310 kb on + strand, within Psest_0932 at 1010.310 kb on + strand, within Psest_0932 at 1010.310 kb on + strand, within Psest_0932 at 1010.311 kb on - strand, within Psest_0932 at 1010.311 kb on - strand, within Psest_0932 at 1010.396 kb on + strand, within Psest_0932 at 1010.397 kb on - strand, within Psest_0932 at 1010.397 kb on - strand, within Psest_0932 at 1010.401 kb on - strand, within Psest_0932 at 1010.401 kb on - strand, within Psest_0932 at 1010.401 kb on - strand, within Psest_0932 at 1010.401 kb on - strand, within Psest_0932 at 1010.407 kb on - strand, within Psest_0932 at 1010.541 kb on + strand, within Psest_0932 at 1010.541 kb on + strand, within Psest_0932 at 1010.542 kb on - strand, within Psest_0932 at 1010.545 kb on - strand, within Psest_0932 at 1010.553 kb on - strand, within Psest_0932 at 1010.559 kb on - strand, within Psest_0932 at 1010.559 kb on - strand, within Psest_0932 at 1010.730 kb on + strand at 1010.731 kb on - strand at 1010.747 kb on + strand at 1010.818 kb on - strand at 1010.818 kb on - strand at 1010.818 kb on - strand at 1010.853 kb on + strand at 1010.861 kb on + strand at 1010.862 kb on - strand at 1010.881 kb on - strand at 1010.894 kb on - strand at 1010.986 kb on + strand at 1010.986 kb on + strand at 1010.986 kb on + strand at 1010.986 kb on + strand at 1010.987 kb on - strand at 1010.987 kb on - strand at 1010.999 kb on - strand at 1011.055 kb on + strand at 1011.055 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,008,167 - -0.3 1,008,167 - +0.3 1,008,219 - -0.1 1,008,219 - -0.9 1,008,343 + Psest_0930 0.24 +0.4 1,008,343 + Psest_0930 0.24 -2.2 1,008,344 - Psest_0930 0.24 -0.3 1,008,344 - Psest_0930 0.24 +0.1 1,008,385 + Psest_0930 0.30 +1.8 1,008,385 + Psest_0930 0.30 +0.7 1,008,386 - Psest_0930 0.30 +1.0 1,008,395 + Psest_0930 0.32 +1.1 1,008,395 + Psest_0930 0.32 +1.4 1,008,395 + Psest_0930 0.32 -2.3 1,008,395 + Psest_0930 0.32 -0.7 1,008,395 + Psest_0930 0.32 +1.7 1,008,396 - Psest_0930 0.32 +1.4 1,008,396 - Psest_0930 0.32 +0.4 1,008,396 - Psest_0930 0.32 +0.1 1,008,396 - Psest_0930 0.32 -0.7 1,008,483 + Psest_0930 0.45 +0.4 1,008,483 + Psest_0930 0.45 -0.4 1,008,483 + Psest_0930 0.45 -0.7 1,008,484 - Psest_0930 0.45 -2.1 1,008,484 - Psest_0930 0.45 +1.1 1,008,484 - Psest_0930 0.45 -1.4 1,008,557 + Psest_0930 0.56 -0.3 1,008,561 + Psest_0930 0.57 +1.5 1,008,561 + Psest_0930 0.57 -2.8 1,008,561 + Psest_0930 0.57 -1.5 1,008,562 - Psest_0930 0.57 -0.1 1,008,562 - Psest_0930 0.57 +0.4 1,008,562 - Psest_0930 0.57 +0.9 1,008,562 - Psest_0930 0.57 +2.0 1,008,617 + Psest_0930 0.66 +0.8 1,008,617 + Psest_0930 0.66 -0.3 1,008,617 + Psest_0930 0.66 -0.4 1,008,617 + Psest_0930 0.66 -0.4 1,008,617 + Psest_0930 0.66 -0.3 1,008,617 + Psest_0930 0.66 +0.6 1,008,617 + Psest_0930 0.66 -1.0 1,008,617 + Psest_0930 0.66 +0.1 1,008,617 + Psest_0930 0.66 +0.1 1,008,618 - Psest_0930 0.66 -1.7 1,008,618 - Psest_0930 0.66 +0.1 1,008,618 - Psest_0930 0.66 -1.3 1,008,618 - Psest_0930 0.66 -0.6 1,008,618 - Psest_0930 0.66 +0.3 1,008,618 - Psest_0930 0.66 +0.1 1,008,618 - Psest_0930 0.66 +0.7 1,008,618 - Psest_0930 0.66 -0.1 1,008,618 - Psest_0930 0.66 +0.8 1,008,736 + Psest_0930 0.84 -0.5 1,008,835 + +0.2 1,008,835 + +0.1 1,008,835 + +0.0 1,008,835 + -0.4 1,008,835 + -0.7 1,008,836 - +0.3 1,008,836 - -1.2 1,008,839 + +0.4 1,008,857 - -0.2 1,008,942 + -0.1 1,008,994 - +0.8 1,009,057 + -0.8 1,009,059 + +0.3 1,009,060 - -0.6 1,009,090 + +1.6 1,009,090 + -2.9 1,009,090 + -0.2 1,009,091 - -0.4 1,009,091 - -0.1 1,009,119 + -0.7 1,009,119 + -0.2 1,009,119 + +0.2 1,009,120 - -0.1 1,009,120 - +0.5 1,009,120 - +0.2 1,009,120 - -0.0 1,009,120 - +1.3 1,009,120 - -1.9 1,009,123 + -0.5 1,009,123 + -0.6 1,009,124 - -0.4 1,009,124 - +0.8 1,009,183 + Psest_0931 0.11 +0.5 1,009,238 + Psest_0931 0.17 -0.3 1,009,238 + Psest_0931 0.17 +0.1 1,009,238 + Psest_0931 0.17 -0.7 1,009,238 + Psest_0931 0.17 +0.7 1,009,239 - Psest_0931 0.17 +1.2 1,009,239 - Psest_0931 0.17 -0.6 1,009,239 - Psest_0931 0.17 +0.7 1,009,239 - Psest_0931 0.17 -0.3 1,009,273 + Psest_0931 0.20 -0.6 1,009,273 + Psest_0931 0.20 -1.9 1,009,273 + Psest_0931 0.20 -2.4 1,009,273 + Psest_0931 0.20 +0.2 1,009,273 + Psest_0931 0.20 -0.2 1,009,273 + Psest_0931 0.20 -0.1 1,009,274 - Psest_0931 0.20 +1.1 1,009,274 - Psest_0931 0.20 -0.9 1,009,274 - Psest_0931 0.20 -0.1 1,009,274 - Psest_0931 0.20 -0.7 1,009,274 - Psest_0931 0.20 +0.6 1,009,274 - Psest_0931 0.20 +0.7 1,009,274 - Psest_0931 0.20 -0.1 1,009,327 + Psest_0931 0.26 -0.3 1,009,327 + Psest_0931 0.26 -0.6 1,009,372 + Psest_0931 0.30 -0.5 1,009,372 + Psest_0931 0.30 +0.5 1,009,372 + Psest_0931 0.30 -0.7 1,009,372 + Psest_0931 0.30 -0.5 1,009,372 + Psest_0931 0.30 -0.2 1,009,373 - Psest_0931 0.30 -0.1 1,009,373 - Psest_0931 0.30 -1.0 1,009,378 - Psest_0931 0.31 +1.3 1,009,387 - Psest_0931 0.32 -1.4 1,009,387 - Psest_0931 0.32 -2.1 1,009,387 - Psest_0931 0.32 -1.1 1,009,476 + Psest_0931 0.41 -0.7 1,009,499 + Psest_0931 0.43 -0.3 1,009,500 - Psest_0931 0.43 +0.4 1,009,606 + Psest_0931 0.54 -3.0 1,009,669 - Psest_0931 0.60 -1.7 1,009,669 - Psest_0931 0.60 +1.0 1,009,669 - Psest_0931 0.60 -0.7 1,009,685 - Psest_0931 0.62 +0.3 1,009,787 - Psest_0931 0.72 -0.4 1,009,802 - Psest_0931 0.74 -0.0 1,009,826 + Psest_0931 0.76 +0.1 1,009,827 - Psest_0931 0.76 -1.7 1,009,918 - Psest_0931 0.86 -0.7 1,009,934 + Psest_0931 0.87 -0.4 1,009,935 - Psest_0931 0.87 +0.2 1,009,935 - Psest_0931 0.87 -2.2 1,009,935 - Psest_0931 0.87 -1.6 1,009,935 - Psest_0931 0.87 -0.7 1,009,935 - Psest_0931 0.87 -0.5 1,009,935 - Psest_0931 0.87 -0.2 1,009,949 + Psest_0931 0.89 -0.3 1,009,949 + Psest_0931 0.89 -0.4 1,009,950 - Psest_0931 0.89 -0.1 1,009,950 - Psest_0931 0.89 +1.2 1,009,950 - Psest_0931 0.89 +0.8 1,010,006 - -0.0 1,010,029 - -1.3 1,010,029 - -0.2 1,010,029 - -0.5 1,010,040 + +1.2 1,010,245 + Psest_0932 0.22 -0.2 1,010,246 - Psest_0932 0.22 -0.2 1,010,246 - Psest_0932 0.22 -1.7 1,010,246 - Psest_0932 0.22 -0.9 1,010,246 - Psest_0932 0.22 -0.9 1,010,310 + Psest_0932 0.33 +0.5 1,010,310 + Psest_0932 0.33 -0.4 1,010,310 + Psest_0932 0.33 +0.3 1,010,310 + Psest_0932 0.33 +0.2 1,010,311 - Psest_0932 0.33 -0.3 1,010,311 - Psest_0932 0.33 +0.2 1,010,396 + Psest_0932 0.46 +1.3 1,010,397 - Psest_0932 0.46 -0.5 1,010,397 - Psest_0932 0.46 +0.2 1,010,401 - Psest_0932 0.47 +0.5 1,010,401 - Psest_0932 0.47 -0.6 1,010,401 - Psest_0932 0.47 +0.4 1,010,401 - Psest_0932 0.47 +0.6 1,010,407 - Psest_0932 0.48 +0.7 1,010,541 + Psest_0932 0.69 -1.1 1,010,541 + Psest_0932 0.69 -0.1 1,010,542 - Psest_0932 0.69 -0.6 1,010,545 - Psest_0932 0.70 +0.3 1,010,553 - Psest_0932 0.71 -2.4 1,010,559 - Psest_0932 0.72 +1.3 1,010,559 - Psest_0932 0.72 -0.1 1,010,730 + +0.1 1,010,731 - -0.2 1,010,747 + -1.6 1,010,818 - -1.0 1,010,818 - +0.4 1,010,818 - +0.8 1,010,853 + -0.5 1,010,861 + -0.4 1,010,862 - +0.9 1,010,881 - -0.9 1,010,894 - +0.2 1,010,986 + -0.7 1,010,986 + +0.4 1,010,986 + -0.5 1,010,986 + +1.4 1,010,987 - +0.2 1,010,987 - +0.3 1,010,999 - -0.5 1,011,055 + +0.2 1,011,055 + -0.7
Or see this region's nucleotide sequence