Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0888 and Psest_0889 are separated by 185 nucleotides Psest_0889 and Psest_0890 are separated by 43 nucleotides Psest_0890 and Psest_0891 are separated by 59 nucleotides Psest_0891 and Psest_0892 are separated by 60 nucleotides
Psest_0888: Psest_0888 - Uncharacterized membrane-anchored protein conserved in bacteria, at 964,958 to 966,293
_0888
Psest_0889: Psest_0889 - Lactoylglutathione lyase and related lyases, at 966,479 to 966,877
_0889
Psest_0890: Psest_0890 - S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase, at 966,921 to 968,030
_0890
Psest_0891: Psest_0891 - Uncharacterized protein conserved in bacteria, at 968,090 to 968,314
_0891
Psest_0892: Psest_0892 - Short-chain alcohol dehydrogenase of unknown specificity, at 968,375 to 969,154
_0892
Position (kb)
966
967
968
969 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 965.974 kb on + strand, within Psest_0888 at 965.974 kb on + strand, within Psest_0888 at 965.974 kb on + strand, within Psest_0888 at 965.974 kb on + strand, within Psest_0888 at 965.974 kb on + strand, within Psest_0888 at 965.974 kb on + strand, within Psest_0888 at 965.974 kb on + strand, within Psest_0888 at 965.974 kb on + strand, within Psest_0888 at 965.974 kb on + strand, within Psest_0888 at 965.975 kb on - strand, within Psest_0888 at 965.975 kb on - strand, within Psest_0888 at 965.975 kb on - strand, within Psest_0888 at 965.975 kb on - strand, within Psest_0888 at 965.975 kb on - strand, within Psest_0888 at 965.975 kb on - strand, within Psest_0888 at 965.975 kb on - strand, within Psest_0888 at 965.975 kb on - strand, within Psest_0888 at 965.975 kb on - strand, within Psest_0888 at 965.975 kb on - strand, within Psest_0888 at 965.975 kb on - strand, within Psest_0888 at 965.975 kb on - strand, within Psest_0888 at 965.975 kb on - strand, within Psest_0888 at 965.978 kb on + strand, within Psest_0888 at 965.978 kb on + strand, within Psest_0888 at 965.979 kb on - strand, within Psest_0888 at 965.979 kb on - strand, within Psest_0888 at 965.979 kb on - strand, within Psest_0888 at 965.979 kb on - strand, within Psest_0888 at 965.979 kb on - strand, within Psest_0888 at 965.979 kb on - strand, within Psest_0888 at 965.979 kb on - strand, within Psest_0888 at 965.979 kb on - strand, within Psest_0888 at 965.979 kb on - strand, within Psest_0888 at 966.081 kb on + strand, within Psest_0888 at 966.081 kb on + strand, within Psest_0888 at 966.081 kb on + strand, within Psest_0888 at 966.081 kb on + strand, within Psest_0888 at 966.082 kb on - strand, within Psest_0888 at 966.082 kb on - strand, within Psest_0888 at 966.144 kb on + strand, within Psest_0888 at 966.144 kb on + strand, within Psest_0888 at 966.144 kb on + strand, within Psest_0888 at 966.145 kb on - strand, within Psest_0888 at 966.145 kb on - strand, within Psest_0888 at 966.145 kb on - strand, within Psest_0888 at 966.145 kb on - strand, within Psest_0888 at 966.145 kb on - strand, within Psest_0888 at 966.145 kb on - strand, within Psest_0888 at 966.145 kb on - strand, within Psest_0888 at 966.146 kb on + strand, within Psest_0888 at 966.270 kb on - strand at 966.270 kb on - strand at 966.414 kb on - strand at 966.428 kb on - strand at 966.430 kb on - strand at 966.430 kb on - strand at 966.430 kb on - strand at 966.430 kb on - strand at 966.430 kb on - strand at 966.430 kb on - strand at 966.432 kb on + strand at 966.433 kb on - strand at 966.433 kb on - strand at 966.433 kb on - strand at 966.433 kb on - strand at 966.492 kb on + strand at 966.492 kb on + strand at 966.493 kb on - strand at 966.528 kb on + strand, within Psest_0889 at 966.529 kb on - strand, within Psest_0889 at 966.529 kb on - strand, within Psest_0889 at 966.529 kb on - strand, within Psest_0889 at 966.529 kb on - strand, within Psest_0889 at 966.537 kb on + strand, within Psest_0889 at 966.537 kb on + strand, within Psest_0889 at 966.537 kb on + strand, within Psest_0889 at 966.537 kb on + strand, within Psest_0889 at 966.537 kb on + strand, within Psest_0889 at 966.537 kb on + strand, within Psest_0889 at 966.538 kb on - strand, within Psest_0889 at 966.538 kb on - strand, within Psest_0889 at 966.731 kb on + strand, within Psest_0889 at 966.792 kb on + strand, within Psest_0889 at 966.792 kb on + strand, within Psest_0889 at 966.793 kb on - strand, within Psest_0889 at 966.793 kb on - strand, within Psest_0889 at 966.881 kb on + strand at 966.881 kb on + strand at 966.882 kb on - strand at 966.882 kb on - strand at 966.882 kb on - strand at 966.882 kb on - strand at 967.600 kb on + strand, within Psest_0890 at 967.601 kb on - strand, within Psest_0890 at 967.627 kb on + strand, within Psest_0890 at 967.627 kb on + strand, within Psest_0890 at 967.627 kb on + strand, within Psest_0890 at 967.627 kb on + strand, within Psest_0890 at 967.628 kb on - strand, within Psest_0890 at 967.628 kb on - strand, within Psest_0890 at 967.628 kb on - strand, within Psest_0890 at 967.628 kb on - strand, within Psest_0890 at 967.628 kb on - strand, within Psest_0890 at 967.633 kb on + strand, within Psest_0890 at 967.634 kb on - strand, within Psest_0890 at 967.634 kb on - strand, within Psest_0890 at 967.634 kb on - strand, within Psest_0890 at 967.634 kb on - strand, within Psest_0890 at 967.651 kb on + strand, within Psest_0890 at 967.651 kb on + strand, within Psest_0890 at 967.651 kb on + strand, within Psest_0890 at 967.651 kb on + strand, within Psest_0890 at 967.651 kb on + strand, within Psest_0890 at 967.652 kb on - strand, within Psest_0890 at 967.768 kb on + strand, within Psest_0890 at 967.769 kb on - strand, within Psest_0890 at 968.045 kb on + strand at 968.045 kb on + strand at 968.046 kb on - strand at 968.048 kb on + strand at 968.048 kb on + strand at 968.048 kb on + strand at 968.049 kb on - strand at 968.049 kb on - strand at 968.049 kb on - strand at 968.049 kb on - strand at 968.049 kb on - strand at 968.049 kb on - strand at 968.049 kb on - strand at 968.052 kb on + strand at 968.053 kb on - strand at 968.053 kb on - strand at 968.053 kb on - strand at 968.057 kb on - strand at 968.057 kb on - strand at 968.092 kb on - strand at 968.101 kb on - strand at 968.101 kb on - strand at 968.101 kb on - strand at 968.187 kb on + strand, within Psest_0891 at 968.187 kb on + strand, within Psest_0891 at 968.187 kb on + strand, within Psest_0891 at 968.187 kb on + strand, within Psest_0891 at 968.187 kb on + strand, within Psest_0891 at 968.187 kb on + strand, within Psest_0891 at 968.188 kb on - strand, within Psest_0891 at 968.188 kb on - strand, within Psest_0891 at 968.188 kb on - strand, within Psest_0891 at 968.188 kb on - strand, within Psest_0891 at 968.188 kb on - strand, within Psest_0891 at 968.188 kb on - strand, within Psest_0891 at 968.188 kb on - strand, within Psest_0891 at 968.188 kb on - strand, within Psest_0891 at 968.188 kb on - strand, within Psest_0891 at 968.335 kb on + strand at 968.377 kb on - strand at 968.413 kb on + strand at 968.490 kb on + strand, within Psest_0892 at 968.490 kb on + strand, within Psest_0892 at 968.490 kb on + strand, within Psest_0892 at 968.490 kb on + strand, within Psest_0892 at 968.490 kb on + strand, within Psest_0892 at 968.491 kb on - strand, within Psest_0892 at 968.491 kb on - strand, within Psest_0892 at 968.491 kb on - strand, within Psest_0892 at 968.491 kb on - strand, within Psest_0892 at 968.491 kb on - strand, within Psest_0892 at 968.491 kb on - strand, within Psest_0892 at 968.620 kb on + strand, within Psest_0892 at 968.621 kb on - strand, within Psest_0892 at 968.649 kb on + strand, within Psest_0892 at 968.758 kb on - strand, within Psest_0892 at 968.780 kb on - strand, within Psest_0892 at 968.858 kb on + strand, within Psest_0892 at 968.859 kb on - strand, within Psest_0892 at 968.859 kb on - strand, within Psest_0892 at 968.859 kb on - strand, within Psest_0892 at 968.859 kb on - strand, within Psest_0892 at 968.859 kb on - strand, within Psest_0892 at 968.859 kb on - strand, within Psest_0892 at 968.859 kb on - strand, within Psest_0892 at 968.859 kb on - strand, within Psest_0892 at 968.859 kb on - strand, within Psest_0892 at 968.859 kb on - strand, within Psest_0892 at 968.874 kb on + strand, within Psest_0892 at 968.874 kb on + strand, within Psest_0892 at 968.875 kb on - strand, within Psest_0892 at 968.923 kb on + strand, within Psest_0892 at 968.923 kb on + strand, within Psest_0892 at 968.923 kb on + strand, within Psest_0892 at 968.924 kb on - strand, within Psest_0892 at 968.924 kb on - strand, within Psest_0892 at 968.924 kb on - strand, within Psest_0892 at 968.992 kb on + strand, within Psest_0892 at 968.993 kb on - strand, within Psest_0892 at 969.018 kb on - strand, within Psest_0892
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 965,974 + Psest_0888 0.76 -0.5 965,974 + Psest_0888 0.76 +0.1 965,974 + Psest_0888 0.76 +0.1 965,974 + Psest_0888 0.76 -0.4 965,974 + Psest_0888 0.76 +0.3 965,974 + Psest_0888 0.76 +0.4 965,974 + Psest_0888 0.76 -0.8 965,974 + Psest_0888 0.76 +0.1 965,974 + Psest_0888 0.76 +0.1 965,975 - Psest_0888 0.76 -0.4 965,975 - Psest_0888 0.76 -1.6 965,975 - Psest_0888 0.76 -1.3 965,975 - Psest_0888 0.76 +0.1 965,975 - Psest_0888 0.76 +0.6 965,975 - Psest_0888 0.76 -1.1 965,975 - Psest_0888 0.76 +0.8 965,975 - Psest_0888 0.76 -1.6 965,975 - Psest_0888 0.76 +1.2 965,975 - Psest_0888 0.76 -0.1 965,975 - Psest_0888 0.76 +0.3 965,975 - Psest_0888 0.76 +0.5 965,975 - Psest_0888 0.76 +0.2 965,978 + Psest_0888 0.76 +0.7 965,978 + Psest_0888 0.76 +0.4 965,979 - Psest_0888 0.76 +0.1 965,979 - Psest_0888 0.76 -0.1 965,979 - Psest_0888 0.76 -1.6 965,979 - Psest_0888 0.76 +0.5 965,979 - Psest_0888 0.76 +0.6 965,979 - Psest_0888 0.76 -1.3 965,979 - Psest_0888 0.76 -0.3 965,979 - Psest_0888 0.76 -0.5 965,979 - Psest_0888 0.76 -0.6 966,081 + Psest_0888 0.84 +2.0 966,081 + Psest_0888 0.84 -0.4 966,081 + Psest_0888 0.84 -0.1 966,081 + Psest_0888 0.84 -0.6 966,082 - Psest_0888 0.84 +0.4 966,082 - Psest_0888 0.84 -1.3 966,144 + Psest_0888 0.89 -1.1 966,144 + Psest_0888 0.89 +0.5 966,144 + Psest_0888 0.89 -0.4 966,145 - Psest_0888 0.89 +0.1 966,145 - Psest_0888 0.89 -0.5 966,145 - Psest_0888 0.89 +0.2 966,145 - Psest_0888 0.89 -0.0 966,145 - Psest_0888 0.89 -1.1 966,145 - Psest_0888 0.89 +0.5 966,145 - Psest_0888 0.89 -0.1 966,146 + Psest_0888 0.89 -1.2 966,270 - -0.9 966,270 - +0.3 966,414 - +0.0 966,428 - +0.7 966,430 - +1.1 966,430 - +0.2 966,430 - +0.9 966,430 - -0.1 966,430 - +0.4 966,430 - +1.2 966,432 + +0.4 966,433 - +1.4 966,433 - +0.1 966,433 - +0.7 966,433 - +0.2 966,492 + -1.6 966,492 + +1.6 966,493 - +0.3 966,528 + Psest_0889 0.12 +0.8 966,529 - Psest_0889 0.13 +0.6 966,529 - Psest_0889 0.13 +2.2 966,529 - Psest_0889 0.13 -0.7 966,529 - Psest_0889 0.13 +0.6 966,537 + Psest_0889 0.15 -0.5 966,537 + Psest_0889 0.15 +0.9 966,537 + Psest_0889 0.15 +2.0 966,537 + Psest_0889 0.15 +0.1 966,537 + Psest_0889 0.15 -0.3 966,537 + Psest_0889 0.15 +0.3 966,538 - Psest_0889 0.15 +0.2 966,538 - Psest_0889 0.15 +0.0 966,731 + Psest_0889 0.63 +0.2 966,792 + Psest_0889 0.78 -0.8 966,792 + Psest_0889 0.78 -0.1 966,793 - Psest_0889 0.79 +1.6 966,793 - Psest_0889 0.79 +0.1 966,881 + -0.7 966,881 + -1.7 966,882 - -0.8 966,882 - -0.9 966,882 - -1.1 966,882 - -0.2 967,600 + Psest_0890 0.61 -0.8 967,601 - Psest_0890 0.61 +0.0 967,627 + Psest_0890 0.64 +0.2 967,627 + Psest_0890 0.64 +0.1 967,627 + Psest_0890 0.64 -0.0 967,627 + Psest_0890 0.64 -1.6 967,628 - Psest_0890 0.64 +0.7 967,628 - Psest_0890 0.64 +0.1 967,628 - Psest_0890 0.64 -0.6 967,628 - Psest_0890 0.64 -0.9 967,628 - Psest_0890 0.64 -0.9 967,633 + Psest_0890 0.64 -0.0 967,634 - Psest_0890 0.64 -2.9 967,634 - Psest_0890 0.64 -0.9 967,634 - Psest_0890 0.64 +0.0 967,634 - Psest_0890 0.64 -1.2 967,651 + Psest_0890 0.66 -0.6 967,651 + Psest_0890 0.66 -0.1 967,651 + Psest_0890 0.66 +0.0 967,651 + Psest_0890 0.66 +0.2 967,651 + Psest_0890 0.66 +1.2 967,652 - Psest_0890 0.66 +1.0 967,768 + Psest_0890 0.76 -0.5 967,769 - Psest_0890 0.76 -0.7 968,045 + -0.7 968,045 + +0.1 968,046 - -0.2 968,048 + -1.5 968,048 + +0.7 968,048 + -0.8 968,049 - +1.0 968,049 - +0.2 968,049 - +0.4 968,049 - +0.1 968,049 - -0.8 968,049 - +0.6 968,049 - +1.7 968,052 + +0.2 968,053 - -1.6 968,053 - +0.3 968,053 - -0.1 968,057 - +0.9 968,057 - -1.0 968,092 - +0.8 968,101 - -0.7 968,101 - +0.3 968,101 - +0.6 968,187 + Psest_0891 0.43 +0.3 968,187 + Psest_0891 0.43 +1.2 968,187 + Psest_0891 0.43 +1.2 968,187 + Psest_0891 0.43 +0.5 968,187 + Psest_0891 0.43 +0.5 968,187 + Psest_0891 0.43 +0.7 968,188 - Psest_0891 0.44 -0.1 968,188 - Psest_0891 0.44 +0.4 968,188 - Psest_0891 0.44 -0.1 968,188 - Psest_0891 0.44 +1.4 968,188 - Psest_0891 0.44 -0.3 968,188 - Psest_0891 0.44 +0.1 968,188 - Psest_0891 0.44 -0.1 968,188 - Psest_0891 0.44 -0.5 968,188 - Psest_0891 0.44 +0.6 968,335 + +1.3 968,377 - +0.4 968,413 + +2.2 968,490 + Psest_0892 0.15 +0.6 968,490 + Psest_0892 0.15 -1.3 968,490 + Psest_0892 0.15 -0.2 968,490 + Psest_0892 0.15 -0.0 968,490 + Psest_0892 0.15 +0.2 968,491 - Psest_0892 0.15 -0.6 968,491 - Psest_0892 0.15 +0.2 968,491 - Psest_0892 0.15 +0.4 968,491 - Psest_0892 0.15 -0.5 968,491 - Psest_0892 0.15 +0.1 968,491 - Psest_0892 0.15 -1.0 968,620 + Psest_0892 0.31 -1.1 968,621 - Psest_0892 0.32 -0.1 968,649 + Psest_0892 0.35 +0.5 968,758 - Psest_0892 0.49 +0.5 968,780 - Psest_0892 0.52 +0.4 968,858 + Psest_0892 0.62 -0.8 968,859 - Psest_0892 0.62 +0.4 968,859 - Psest_0892 0.62 -0.3 968,859 - Psest_0892 0.62 -0.4 968,859 - Psest_0892 0.62 -0.0 968,859 - Psest_0892 0.62 -2.2 968,859 - Psest_0892 0.62 -0.8 968,859 - Psest_0892 0.62 -0.6 968,859 - Psest_0892 0.62 -0.6 968,859 - Psest_0892 0.62 +0.7 968,859 - Psest_0892 0.62 +0.4 968,874 + Psest_0892 0.64 +0.5 968,874 + Psest_0892 0.64 +1.2 968,875 - Psest_0892 0.64 -0.3 968,923 + Psest_0892 0.70 +0.1 968,923 + Psest_0892 0.70 +0.0 968,923 + Psest_0892 0.70 +1.0 968,924 - Psest_0892 0.70 -0.5 968,924 - Psest_0892 0.70 -0.6 968,924 - Psest_0892 0.70 +0.4 968,992 + Psest_0892 0.79 -2.2 968,993 - Psest_0892 0.79 -0.3 969,018 - Psest_0892 0.82 +0.2
Or see this region's nucleotide sequence