Experiment: m.b. A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c05840 and PGA1_c05850 are separated by 361 nucleotides PGA1_c05850 and PGA1_c05860 overlap by 4 nucleotides PGA1_c05860 and PGA1_c05870 are separated by 99 nucleotides
PGA1_c05840: PGA1_c05840 - Uncharacterized conserved small protein, at 598,729 to 598,947
_c05840
PGA1_c05850: PGA1_c05850 - crossover junction endodeoxyribonuclease RuvC, at 599,309 to 599,806
_c05850
PGA1_c05860: PGA1_c05860 - holliday junction ATP-dependent DNA helicase RuvA, at 599,803 to 600,492
_c05860
PGA1_c05870: PGA1_c05870 - holliday junction ATP-dependent DNA helicase RuvB, at 600,592 to 601,641
_c05870
Position (kb)
599
600
601 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 598.943 kb on + strand at 599.009 kb on - strand at 599.103 kb on + strand at 599.111 kb on - strand at 599.144 kb on - strand at 599.291 kb on + strand at 599.296 kb on + strand at 599.304 kb on - strand at 599.309 kb on - strand at 599.309 kb on - strand at 599.357 kb on - strand at 599.562 kb on + strand, within PGA1_c05850 at 599.562 kb on - strand, within PGA1_c05850 at 599.577 kb on + strand, within PGA1_c05850 at 599.759 kb on - strand at 599.784 kb on + strand at 599.812 kb on + strand at 599.894 kb on - strand, within PGA1_c05860 at 599.921 kb on + strand, within PGA1_c05860 at 599.941 kb on + strand, within PGA1_c05860 at 599.976 kb on - strand, within PGA1_c05860 at 600.197 kb on - strand, within PGA1_c05860 at 600.203 kb on - strand, within PGA1_c05860 at 600.461 kb on - strand at 600.515 kb on - strand at 600.518 kb on + strand at 600.556 kb on + strand at 600.726 kb on - strand, within PGA1_c05870 at 600.881 kb on - strand, within PGA1_c05870 at 600.891 kb on - strand, within PGA1_c05870 at 601.124 kb on - strand, within PGA1_c05870 at 601.351 kb on - strand, within PGA1_c05870 at 601.378 kb on + strand, within PGA1_c05870 at 601.461 kb on - strand, within PGA1_c05870
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. A22 0.02 mg/ml remove 598,943 + -1.7 599,009 - -0.4 599,103 + +0.2 599,111 - -1.6 599,144 - -0.4 599,291 + -1.4 599,296 + +0.9 599,304 - +0.6 599,309 - -1.0 599,309 - -0.2 599,357 - +3.1 599,562 + PGA1_c05850 0.51 +4.1 599,562 - PGA1_c05850 0.51 -1.0 599,577 + PGA1_c05850 0.54 -1.0 599,759 - -0.8 599,784 + +1.9 599,812 + -2.5 599,894 - PGA1_c05860 0.13 -1.4 599,921 + PGA1_c05860 0.17 -0.4 599,941 + PGA1_c05860 0.20 -0.4 599,976 - PGA1_c05860 0.25 -0.7 600,197 - PGA1_c05860 0.57 -0.4 600,203 - PGA1_c05860 0.58 -1.6 600,461 - -1.4 600,515 - -0.5 600,518 + +0.1 600,556 + +0.6 600,726 - PGA1_c05870 0.13 -1.0 600,881 - PGA1_c05870 0.28 +0.9 600,891 - PGA1_c05870 0.28 -0.2 601,124 - PGA1_c05870 0.51 -2.0 601,351 - PGA1_c05870 0.72 -1.5 601,378 + PGA1_c05870 0.75 -0.4 601,461 - PGA1_c05870 0.83 -0.2
Or see this region's nucleotide sequence