Experiment: m.b. A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c00030 and PGA1_c00040 overlap by 4 nucleotides PGA1_c00040 and PGA1_c00050 are separated by 14 nucleotides PGA1_c00050 and PGA1_c00060 are separated by 100 nucleotides PGA1_c00060 and PGA1_c00070 are separated by 85 nucleotides
PGA1_c00030: PGA1_c00030 - DNA replication and repair protein RecF, at 3,057 to 4,154
_c00030
PGA1_c00040: PGA1_c00040 - putative LysE type translocator, at 4,151 to 4,771
_c00040
PGA1_c00050: PGA1_c00050 - putative transcriptional regulator, tetR family, at 4,786 to 5,385
_c00050
PGA1_c00060: PGA1_c00060 - putative NAD(P)H dehydrogenase (quinone), at 5,486 to 6,079
_c00060
PGA1_c00070: PGA1_c00070 - DNA gyrase subunit GyrB, at 6,165 to 8,582
_c00070
Position (kb) 4
5
6 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 3.799 kb on + strand, within PGA1_c00030 at 3.807 kb on - strand, within PGA1_c00030 at 3.807 kb on - strand, within PGA1_c00030 at 3.831 kb on - strand, within PGA1_c00030 at 3.848 kb on + strand, within PGA1_c00030 at 3.848 kb on + strand, within PGA1_c00030 at 3.897 kb on + strand, within PGA1_c00030 at 4.009 kb on - strand, within PGA1_c00030 at 4.035 kb on - strand, within PGA1_c00030 at 4.046 kb on + strand at 4.066 kb on + strand at 4.066 kb on + strand at 4.066 kb on + strand at 4.066 kb on + strand at 4.074 kb on - strand at 4.074 kb on - strand at 4.074 kb on - strand at 4.098 kb on + strand at 4.103 kb on + strand at 4.111 kb on + strand at 4.114 kb on - strand at 4.132 kb on + strand at 4.195 kb on - strand at 4.212 kb on - strand at 4.235 kb on - strand, within PGA1_c00040 at 4.290 kb on - strand, within PGA1_c00040 at 4.369 kb on + strand, within PGA1_c00040 at 4.402 kb on - strand, within PGA1_c00040 at 4.480 kb on + strand, within PGA1_c00040 at 4.522 kb on - strand, within PGA1_c00040 at 4.580 kb on + strand, within PGA1_c00040 at 4.580 kb on + strand, within PGA1_c00040 at 4.581 kb on - strand, within PGA1_c00040 at 4.656 kb on - strand, within PGA1_c00040 at 4.656 kb on - strand, within PGA1_c00040 at 4.666 kb on - strand, within PGA1_c00040 at 4.671 kb on - strand, within PGA1_c00040 at 4.777 kb on + strand at 4.780 kb on - strand at 4.780 kb on - strand at 4.785 kb on + strand at 4.825 kb on + strand at 4.858 kb on + strand, within PGA1_c00050 at 4.873 kb on + strand, within PGA1_c00050 at 4.913 kb on + strand, within PGA1_c00050 at 4.921 kb on - strand, within PGA1_c00050 at 4.921 kb on - strand, within PGA1_c00050 at 4.950 kb on + strand, within PGA1_c00050 at 4.971 kb on + strand, within PGA1_c00050 at 4.979 kb on - strand, within PGA1_c00050 at 5.031 kb on - strand, within PGA1_c00050 at 5.057 kb on - strand, within PGA1_c00050 at 5.068 kb on - strand, within PGA1_c00050 at 5.103 kb on + strand, within PGA1_c00050 at 5.148 kb on - strand, within PGA1_c00050 at 5.152 kb on + strand, within PGA1_c00050 at 5.304 kb on - strand, within PGA1_c00050 at 5.304 kb on - strand, within PGA1_c00050 at 5.367 kb on + strand at 5.367 kb on + strand at 5.373 kb on - strand at 5.373 kb on - strand at 5.375 kb on - strand at 5.409 kb on - strand at 5.472 kb on - strand at 5.480 kb on + strand at 5.482 kb on + strand at 5.490 kb on - strand at 5.490 kb on - strand at 5.525 kb on + strand at 5.578 kb on + strand, within PGA1_c00060 at 5.588 kb on + strand, within PGA1_c00060 at 5.594 kb on - strand, within PGA1_c00060 at 5.594 kb on - strand, within PGA1_c00060 at 5.601 kb on + strand, within PGA1_c00060 at 5.601 kb on + strand, within PGA1_c00060 at 5.707 kb on - strand, within PGA1_c00060 at 5.707 kb on - strand, within PGA1_c00060 at 5.741 kb on - strand, within PGA1_c00060 at 5.743 kb on - strand, within PGA1_c00060 at 5.764 kb on + strand, within PGA1_c00060 at 5.772 kb on - strand, within PGA1_c00060 at 5.876 kb on + strand, within PGA1_c00060 at 5.883 kb on + strand, within PGA1_c00060 at 5.883 kb on + strand, within PGA1_c00060 at 5.892 kb on + strand, within PGA1_c00060 at 5.894 kb on + strand, within PGA1_c00060 at 5.894 kb on + strand, within PGA1_c00060 at 5.904 kb on + strand, within PGA1_c00060 at 5.914 kb on + strand, within PGA1_c00060 at 5.949 kb on + strand, within PGA1_c00060 at 5.978 kb on - strand, within PGA1_c00060 at 5.994 kb on + strand, within PGA1_c00060 at 6.029 kb on + strand at 6.043 kb on + strand at 6.050 kb on + strand at 6.051 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. A22 0.02 mg/ml remove 3,799 + PGA1_c00030 0.68 -0.6 3,807 - PGA1_c00030 0.68 -0.4 3,807 - PGA1_c00030 0.68 -0.7 3,831 - PGA1_c00030 0.70 -1.1 3,848 + PGA1_c00030 0.72 -0.9 3,848 + PGA1_c00030 0.72 +1.1 3,897 + PGA1_c00030 0.77 +0.4 4,009 - PGA1_c00030 0.87 -1.3 4,035 - PGA1_c00030 0.89 -1.6 4,046 + +1.1 4,066 + +0.5 4,066 + +0.4 4,066 + -2.4 4,066 + -1.2 4,074 - +0.2 4,074 - -0.7 4,074 - -0.0 4,098 + -0.4 4,103 + -0.1 4,111 + +0.8 4,114 - -1.8 4,132 + -0.3 4,195 - -0.1 4,212 - +1.0 4,235 - PGA1_c00040 0.14 -0.9 4,290 - PGA1_c00040 0.22 -0.7 4,369 + PGA1_c00040 0.35 -1.0 4,402 - PGA1_c00040 0.40 +0.1 4,480 + PGA1_c00040 0.53 +0.1 4,522 - PGA1_c00040 0.60 -1.0 4,580 + PGA1_c00040 0.69 +0.2 4,580 + PGA1_c00040 0.69 -0.0 4,581 - PGA1_c00040 0.69 -0.1 4,656 - PGA1_c00040 0.81 -1.0 4,656 - PGA1_c00040 0.81 +0.1 4,666 - PGA1_c00040 0.83 -1.7 4,671 - PGA1_c00040 0.84 +1.6 4,777 + -0.7 4,780 - -1.4 4,780 - -0.3 4,785 + +0.1 4,825 + -0.9 4,858 + PGA1_c00050 0.12 -1.0 4,873 + PGA1_c00050 0.14 -1.2 4,913 + PGA1_c00050 0.21 +0.3 4,921 - PGA1_c00050 0.23 -2.9 4,921 - PGA1_c00050 0.23 -1.9 4,950 + PGA1_c00050 0.27 +0.4 4,971 + PGA1_c00050 0.31 -0.7 4,979 - PGA1_c00050 0.32 -1.4 5,031 - PGA1_c00050 0.41 -0.4 5,057 - PGA1_c00050 0.45 -0.2 5,068 - PGA1_c00050 0.47 -0.3 5,103 + PGA1_c00050 0.53 -3.0 5,148 - PGA1_c00050 0.60 +0.1 5,152 + PGA1_c00050 0.61 +0.5 5,304 - PGA1_c00050 0.86 +0.5 5,304 - PGA1_c00050 0.86 +2.1 5,367 + +1.5 5,367 + -0.2 5,373 - -0.6 5,373 - -0.6 5,375 - -2.1 5,409 - -0.1 5,472 - +0.8 5,480 + -0.0 5,482 + -2.2 5,490 - -0.3 5,490 - +0.6 5,525 + -0.3 5,578 + PGA1_c00060 0.15 -0.1 5,588 + PGA1_c00060 0.17 +1.7 5,594 - PGA1_c00060 0.18 +1.1 5,594 - PGA1_c00060 0.18 -0.3 5,601 + PGA1_c00060 0.19 +0.4 5,601 + PGA1_c00060 0.19 -0.2 5,707 - PGA1_c00060 0.37 +0.3 5,707 - PGA1_c00060 0.37 -0.5 5,741 - PGA1_c00060 0.43 -2.7 5,743 - PGA1_c00060 0.43 +1.3 5,764 + PGA1_c00060 0.47 +0.9 5,772 - PGA1_c00060 0.48 -0.3 5,876 + PGA1_c00060 0.66 -0.6 5,883 + PGA1_c00060 0.67 +0.3 5,883 + PGA1_c00060 0.67 -0.4 5,892 + PGA1_c00060 0.68 -0.0 5,894 + PGA1_c00060 0.69 -0.3 5,894 + PGA1_c00060 0.69 +1.6 5,904 + PGA1_c00060 0.70 +0.1 5,914 + PGA1_c00060 0.72 -0.2 5,949 + PGA1_c00060 0.78 -0.6 5,978 - PGA1_c00060 0.83 -0.3 5,994 + PGA1_c00060 0.86 -0.4 6,029 + +0.2 6,043 + -0.5 6,050 + +1.1 6,051 - +1.6
Or see this region's nucleotide sequence