Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0473 and Psest_0474 overlap by 8 nucleotides Psest_0474 and Psest_0475 are separated by 112 nucleotides
Psest_0473: Psest_0473 - Glycerol-3-phosphate dehydrogenase, at 521,763 to 522,938
_0473
Psest_0474: Psest_0474 - Membrane transporters of cations and cationic drugs, at 522,931 to 523,329
_0474
Psest_0475: Psest_0475 - 3-deoxy-D-manno-octulosonic-acid transferase, at 523,442 to 524,710
_0475
Position (kb) 522
523
524 Strain fitness (log2 ratio) -2
-1
0
1 at 522.952 kb on - strand at 522.973 kb on - strand, within Psest_0474 at 522.973 kb on - strand, within Psest_0474 at 522.973 kb on - strand, within Psest_0474 at 522.973 kb on - strand, within Psest_0474 at 522.973 kb on - strand, within Psest_0474 at 522.974 kb on + strand, within Psest_0474 at 522.974 kb on + strand, within Psest_0474 at 522.974 kb on + strand, within Psest_0474 at 522.974 kb on + strand, within Psest_0474 at 522.974 kb on + strand, within Psest_0474 at 522.975 kb on - strand, within Psest_0474 at 522.975 kb on - strand, within Psest_0474 at 522.975 kb on - strand, within Psest_0474 at 522.975 kb on - strand, within Psest_0474 at 522.975 kb on - strand, within Psest_0474 at 522.975 kb on - strand, within Psest_0474 at 522.975 kb on - strand, within Psest_0474 at 522.975 kb on - strand, within Psest_0474 at 523.151 kb on + strand, within Psest_0474 at 523.151 kb on + strand, within Psest_0474 at 523.313 kb on + strand at 523.313 kb on + strand at 523.314 kb on - strand at 523.345 kb on - strand at 523.457 kb on + strand at 523.457 kb on + strand at 523.457 kb on + strand at 523.457 kb on + strand at 523.457 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 522,952 - +0.3 522,973 - Psest_0474 0.11 -1.7 522,973 - Psest_0474 0.11 -1.9 522,973 - Psest_0474 0.11 -1.5 522,973 - Psest_0474 0.11 -0.3 522,973 - Psest_0474 0.11 -0.7 522,974 + Psest_0474 0.11 -0.3 522,974 + Psest_0474 0.11 -0.3 522,974 + Psest_0474 0.11 -0.3 522,974 + Psest_0474 0.11 -0.5 522,974 + Psest_0474 0.11 -1.3 522,975 - Psest_0474 0.11 -0.0 522,975 - Psest_0474 0.11 -1.1 522,975 - Psest_0474 0.11 -0.8 522,975 - Psest_0474 0.11 -0.7 522,975 - Psest_0474 0.11 -0.7 522,975 - Psest_0474 0.11 +0.1 522,975 - Psest_0474 0.11 +0.4 522,975 - Psest_0474 0.11 -0.4 523,151 + Psest_0474 0.55 +0.6 523,151 + Psest_0474 0.55 -1.2 523,313 + +0.3 523,313 + -2.1 523,314 - -1.2 523,345 - -1.2 523,457 + +0.4 523,457 + +0.5 523,457 + -0.1 523,457 + -0.1 523,457 + -0.7
Or see this region's nucleotide sequence