Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3125 and Psest_3126 are separated by 74 nucleotides Psest_3126 and Psest_3127 are separated by 112 nucleotides Psest_3127 and Psest_3128 are separated by 101 nucleotides
Psest_3125: Psest_3125 - threonine synthase, at 3,301,457 to 3,302,866
_3125
Psest_3126: Psest_3126 - Homoserine dehydrogenase, at 3,302,941 to 3,304,245
_3126
Psest_3127: Psest_3127 - Uncharacterized conserved protein, at 3,304,358 to 3,304,846
_3127
Psest_3128: Psest_3128 - Protein-disulfide isomerase, at 3,304,948 to 3,305,673
_3128
Position (kb)
3302
3303
3304
3305 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3302.943 kb on - strand at 3302.943 kb on - strand at 3302.943 kb on - strand at 3304.326 kb on - strand at 3304.486 kb on + strand, within Psest_3127 at 3304.487 kb on - strand, within Psest_3127 at 3304.487 kb on - strand, within Psest_3127 at 3304.487 kb on - strand, within Psest_3127 at 3304.487 kb on - strand, within Psest_3127 at 3304.487 kb on - strand, within Psest_3127 at 3304.487 kb on - strand, within Psest_3127 at 3304.555 kb on + strand, within Psest_3127 at 3304.556 kb on - strand, within Psest_3127 at 3304.556 kb on - strand, within Psest_3127 at 3304.660 kb on - strand, within Psest_3127 at 3304.748 kb on - strand, within Psest_3127 at 3304.764 kb on + strand, within Psest_3127 at 3304.764 kb on + strand, within Psest_3127 at 3304.765 kb on - strand, within Psest_3127 at 3304.765 kb on - strand, within Psest_3127 at 3304.884 kb on + strand at 3304.884 kb on + strand at 3304.884 kb on + strand at 3304.884 kb on + strand at 3304.884 kb on + strand at 3304.884 kb on + strand at 3304.885 kb on - strand at 3304.885 kb on - strand at 3304.885 kb on - strand at 3304.885 kb on - strand at 3304.885 kb on - strand at 3304.885 kb on - strand at 3304.885 kb on - strand at 3304.885 kb on - strand at 3304.917 kb on - strand at 3304.917 kb on - strand at 3305.165 kb on - strand, within Psest_3128 at 3305.165 kb on - strand, within Psest_3128 at 3305.165 kb on - strand, within Psest_3128 at 3305.218 kb on + strand, within Psest_3128 at 3305.218 kb on + strand, within Psest_3128 at 3305.218 kb on + strand, within Psest_3128 at 3305.219 kb on - strand, within Psest_3128 at 3305.219 kb on - strand, within Psest_3128 at 3305.219 kb on - strand, within Psest_3128 at 3305.219 kb on - strand, within Psest_3128 at 3305.223 kb on - strand, within Psest_3128
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 3,302,943 - +0.8 3,302,943 - -0.0 3,302,943 - -1.1 3,304,326 - +0.2 3,304,486 + Psest_3127 0.26 +0.3 3,304,487 - Psest_3127 0.26 +0.9 3,304,487 - Psest_3127 0.26 +1.8 3,304,487 - Psest_3127 0.26 -1.1 3,304,487 - Psest_3127 0.26 -0.7 3,304,487 - Psest_3127 0.26 -0.3 3,304,487 - Psest_3127 0.26 +0.3 3,304,555 + Psest_3127 0.40 -0.2 3,304,556 - Psest_3127 0.40 -0.3 3,304,556 - Psest_3127 0.40 -2.1 3,304,660 - Psest_3127 0.62 +0.1 3,304,748 - Psest_3127 0.80 +0.6 3,304,764 + Psest_3127 0.83 +0.6 3,304,764 + Psest_3127 0.83 -0.5 3,304,765 - Psest_3127 0.83 -0.4 3,304,765 - Psest_3127 0.83 +0.3 3,304,884 + -0.2 3,304,884 + +0.7 3,304,884 + +0.5 3,304,884 + +0.3 3,304,884 + -0.6 3,304,884 + +0.6 3,304,885 - +0.0 3,304,885 - -0.2 3,304,885 - -1.1 3,304,885 - +0.3 3,304,885 - -0.3 3,304,885 - +0.0 3,304,885 - +0.1 3,304,885 - +1.3 3,304,917 - -0.1 3,304,917 - -0.0 3,305,165 - Psest_3128 0.30 +1.7 3,305,165 - Psest_3128 0.30 -0.1 3,305,165 - Psest_3128 0.30 -2.6 3,305,218 + Psest_3128 0.37 -0.8 3,305,218 + Psest_3128 0.37 +0.3 3,305,218 + Psest_3128 0.37 +0.5 3,305,219 - Psest_3128 0.37 +0.0 3,305,219 - Psest_3128 0.37 +0.1 3,305,219 - Psest_3128 0.37 -1.0 3,305,219 - Psest_3128 0.37 -0.2 3,305,223 - Psest_3128 0.38 -2.4
Or see this region's nucleotide sequence