Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3008 and Psest_3009 overlap by 4 nucleotides Psest_3009 and Psest_3010 overlap by 8 nucleotides Psest_3010 and Psest_3011 overlap by 4 nucleotides
Psest_3008: Psest_3008 - cobyric acid synthase CobQ, at 3,196,118 to 3,197,575
_3008
Psest_3009: Psest_3009 - L-threonine-O-3-phosphate decarboxylase, at 3,197,572 to 3,198,561
_3009
Psest_3010: Psest_3010 - cobalamin biosynthesis protein CobD, at 3,198,554 to 3,199,465
_3010
Psest_3011: Psest_3011 - ABC-type Fe3+-hydroxamate transport system, periplasmic component, at 3,199,462 to 3,200,262
_3011
Position (kb)
3197
3198
3199 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3196.754 kb on + strand, within Psest_3008 at 3196.754 kb on + strand, within Psest_3008 at 3196.755 kb on - strand, within Psest_3008 at 3197.260 kb on - strand, within Psest_3008 at 3197.260 kb on - strand, within Psest_3008 at 3198.276 kb on - strand, within Psest_3009 at 3198.467 kb on + strand at 3198.518 kb on + strand at 3198.561 kb on + strand at 3198.561 kb on + strand at 3198.562 kb on - strand at 3198.562 kb on - strand at 3198.562 kb on - strand at 3198.700 kb on - strand, within Psest_3010 at 3198.700 kb on - strand, within Psest_3010 at 3198.837 kb on + strand, within Psest_3010 at 3198.837 kb on + strand, within Psest_3010 at 3198.837 kb on + strand, within Psest_3010 at 3198.837 kb on + strand, within Psest_3010 at 3198.837 kb on + strand, within Psest_3010 at 3198.837 kb on + strand, within Psest_3010 at 3198.837 kb on + strand, within Psest_3010 at 3198.837 kb on + strand, within Psest_3010 at 3198.837 kb on + strand, within Psest_3010 at 3198.837 kb on + strand, within Psest_3010 at 3198.837 kb on + strand, within Psest_3010 at 3198.837 kb on + strand, within Psest_3010 at 3198.837 kb on + strand, within Psest_3010 at 3198.838 kb on - strand, within Psest_3010 at 3198.867 kb on + strand, within Psest_3010 at 3198.867 kb on + strand, within Psest_3010 at 3198.868 kb on - strand, within Psest_3010 at 3198.953 kb on - strand, within Psest_3010 at 3198.997 kb on + strand, within Psest_3010 at 3199.115 kb on + strand, within Psest_3010 at 3199.116 kb on - strand, within Psest_3010 at 3199.116 kb on - strand, within Psest_3010 at 3199.209 kb on + strand, within Psest_3010 at 3199.257 kb on - strand, within Psest_3010 at 3199.510 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 3,196,754 + Psest_3008 0.44 +0.2 3,196,754 + Psest_3008 0.44 -1.4 3,196,755 - Psest_3008 0.44 +0.9 3,197,260 - Psest_3008 0.78 -0.5 3,197,260 - Psest_3008 0.78 -0.2 3,198,276 - Psest_3009 0.71 -2.3 3,198,467 + -0.8 3,198,518 + +0.2 3,198,561 + +0.7 3,198,561 + +0.4 3,198,562 - +2.3 3,198,562 - -0.3 3,198,562 - +0.8 3,198,700 - Psest_3010 0.16 -1.2 3,198,700 - Psest_3010 0.16 +0.6 3,198,837 + Psest_3010 0.31 -0.6 3,198,837 + Psest_3010 0.31 +0.5 3,198,837 + Psest_3010 0.31 +1.2 3,198,837 + Psest_3010 0.31 +0.0 3,198,837 + Psest_3010 0.31 +1.4 3,198,837 + Psest_3010 0.31 -2.4 3,198,837 + Psest_3010 0.31 -1.1 3,198,837 + Psest_3010 0.31 +0.5 3,198,837 + Psest_3010 0.31 +1.6 3,198,837 + Psest_3010 0.31 +0.9 3,198,837 + Psest_3010 0.31 -0.4 3,198,837 + Psest_3010 0.31 -0.4 3,198,837 + Psest_3010 0.31 -1.8 3,198,838 - Psest_3010 0.31 +0.1 3,198,867 + Psest_3010 0.34 +0.6 3,198,867 + Psest_3010 0.34 -0.5 3,198,868 - Psest_3010 0.34 -0.7 3,198,953 - Psest_3010 0.44 -0.0 3,198,997 + Psest_3010 0.49 +0.6 3,199,115 + Psest_3010 0.62 +0.1 3,199,116 - Psest_3010 0.62 -0.0 3,199,116 - Psest_3010 0.62 +0.7 3,199,209 + Psest_3010 0.72 +1.4 3,199,257 - Psest_3010 0.77 +1.3 3,199,510 + -0.1
Or see this region's nucleotide sequence